Abstract：
:Thirty-two Enterobacter cloacae strains that are less susceptible to cefepime than to ceftazidime were collected. This unique phenotype of 8 strains was confirmed using the agar dilution method. OXA1, OXA10, OXA31 and OXA35 were detected in 3, 2, 3, and 2 strains, respectively, whereas all strains were negative for PSE-1 genes. OXA genes were also identified in the plasmid DNA of 5 strains, but only 2 strains were positive in a conjugation experiment. The acrA, acrB and tolC genes were identified in 4, 4 and 6 strains, respectively. Decreased expression of the acrA mRNA and overexpression of the acrB and tolC mRNAs were observed using real-time RT-PCR. Most of the bacteria (n=7) stably expressed the marA gene, which is a regulatory gene in the AcrAB-TolC multidrug efflux system, whereas all strains were negative for ramA. The acrA, acrB, tolC, acrR and marA genes were similar to the genes in reference strains in GenBank, with nucleotide homologies of 96%, 98%, 98%, 98% and 100%, respectively. In conclusion, the mechanism of resistance of Enterobacter cloacae with less susceptibility to cefepime than to ceftazidime is associated with the overexpression of AcrAB-TolC and the production of OXA1, XA10, OXA31 and OXA35.
journal_name
Ann Clin Lab Scijournal_title
Annals of clinical and laboratory scienceauthors
Liu L,Yu J,Tang M,Liu Jsubject
Has Abstractpub_date
2018-05-01 00:00:00pages
355-362issue
3eissn
0091-7370issn
1550-8080pii
48/3/355journal_volume
48pub_type
杂志文章abstract：:We describe a case of relapsed Plasmodium falciparum malaria detected by rapid diagnostic testing (RDT) 19 days before microscopic- or PCR-positive conversion. Three malaria tests gave discrepant results; the malaria smear and polymerase chain reaction (PCR) tests were both negative, while malaria RDT with a commercia...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2010-04-01 00:00:00
abstract：:Qualitative urinary screening procedures were converted to quantitative methods for urinary cystine and homocystine based on the reactions between these amino acids and cyanide-nitroprusside reagents. Cystine and homocystine are quantified by the measurement of absorbances at 521 and 524 nm, respectively. Cyanide-nitr...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
abstract：:In vitro determination of allergen-specific IgE (sIgE) represents an important aid in the diagnosis and treatment of allergy. Improvements in laboratory methodology--the development of second, and now third generation assays for sIgE--have brought about major advances in speed, convenience, performance, and in the sta...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:We examined the results of 1.3 million drug tests performed on patients being monitored for compliance with pain medications and substance abuse rehabilitation to determine if the 2016 CDC prescribing guidelines had any impact on opiate benzodiazepine use. We observed that the combination of the opiate drugs morphine,...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2020-05-01 00:00:00
abstract：OBJECTIVE:This study aims to develop a strategy for distinguishing Yersinia pestis of the Xilingele Plateau ecotype from other biovar of Yersinia pestis using insertion sequences. METHODS:Genome computational analysis was used to discover the positions of insertion sequences unique to Yersinia pestis 91001. PCR was us...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2019-09-01 00:00:00
abstract：BACKGROUND:Long-term use of hormones and immunosuppressive agents is necessary for treating polymyositis (PM)/dermatomyositis (DM) but may cause liver damage. At what point do the costs of treatment outweigh the benefits, and how will the breakeven point be determined? METHODS:Serum muscle enzyme changes in 93 PM/DM p...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2016-05-01 00:00:00
abstract：:Increased red cell lipid peroxidation (LP) occurs in the presence of ionized iron, as well as with various heme moieties and other transition metal ions. In stored erythrocytes, the degree of LP, as determined by plasma malondialdehyde measurements, can be significantly reduced by the addition of various metal chelati...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：:Absorbed lead continuously adds to the lead deposited in the vascularized circumpulpal dentin of the teeth. Thus, this dental tissue is expected to contain a lead concentration which reflects the integrated lead exposure during the time from completion of tooth formation to tooth extraction or shedding. A method has b...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1984-07-01 00:00:00
abstract：:Histologic sections of thyroid glands resected from 12 patients with Hashimoto's thyroiditis have been studied in which areas were present showing clear nuclear changes such as those seen in papillary carcinoma. The patients' ages ranged from 28 to 78 years (mean = 57.3); 11 were women and one was a man. The lesions p...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:We report the case of a 45 year old man who came to Emergency Room of Polyclinic for sudden onset of localized ecchymosis and widespread hematomas. He was subjected to blood count and first level investigations to assess coagulation. Based on the results, second level investigations were performed. Endoscopy of the ga...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2014-07-01 00:00:00
abstract：:We present an adult autopsy case of primary hyperoxaluria type 1. Diagnosis was established with skin biopsy and subsequent genetic analysis one month prior to death. At autopsy, calcium oxalate crystals refringent to polarized light were found systemically. Interestingly, however, calcium oxalate crystals were not id...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2013-07-01 00:00:00
abstract：:The present study reported a rare case with thymic carcinoid as the first manifestation of multiple endocrine neoplasia type 1 (MEN1) syndrome, which presented with gene mutations of the MEN1 and glucokinase regulatory protein (GCKR). In this report, a 40-year-old male was diagnosed as MEN1 syndrome with thymic carcin...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2020-11-01 00:00:00
abstract：:The determination of the T and B subpopulation percentages and lymphocyte blastogenic response functions are useful tools to evaluate and monitor disorders associated with immunodeficiencies, lymphoproliferations, malignancies, infections, inflammations, and autoimmune diseases. Comparative studies of cryopreserved ly...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：:A case of multiple myeloma producing IgD-M component with light chains of lambda type is reported. It involved the skeleton and was complicated by compression of the spinal cord and massive extension to the pleura. The urine contained lambda Bence Jones protein. Under the electron microscope, the neoplastic plasma cel...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1978-05-01 00:00:00
abstract：:In the past, necrotizing vasculitis has been considered to be one of the dominant intrarenal vascular abnormalities in systemic lupus erythematosus (SLE). To test the validity of this statement, 70 consecutive renal biopsies from patients with SLE were reviewed. Light microscopy (LM) and immunofluorescence (IF) studie...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
abstract：:A high performance liquid chromatography has been adapted to the measurement of putrescine, spermidine and spermine in plasma and erythrocytes. Detection was by fluorimetry using o-phthalaldehyde which formed fluorophores with the polyamines. Sensitivity of the method was better than 10 pmol of each polyamine, and rec...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
abstract：:Recently, the current authors reported the presence in normotensive male and female urines of reproducibly measurable levels of naturally occurring substances in partially purified extracts of urine with inhibitory activity like glycyrrhetic acid (GA) towards both 11 beta-hydroxysteroid dehydrogenase (11 beta-OHSD) an...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:This paper is a summary of studies over the past few years that pertain to animal neurotoxins. These toxins are found throughout the animal kingdom. Homologies exist in the structures of these poisons within classes and point to conservation of active sites throughout evolution. In the case of the peptides, invariant ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1975-09-01 00:00:00
abstract：:Although it is widely accepted that patients with immune thrombocytopenia produce platelet antibodies, the demonstration of such antibodies has been difficult and time consuming. A simple and quick enzyme linked immunoassay for platelet antibodies is presented. The platelet associated IgG is coupled with alkaline phos...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
abstract：:The Department of Laboratory Medicine at Asan Medical Center provides a special one-stop service to allow both doctors and outpatients to receive routine outpatient test results in a timely manner within 1 hr. We developed a new Laboratory Information System (LIS)-based monitoring system that records the laboratory tu...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2009-04-01 00:00:00
abstract：:The concentrations of progesterone, cortisol, estradiol, and transcortin binding capacity (TBC) were measured in plasma samples of women during normal pregnancy. Between 10 weeks and 20 weeks gestation, the mean of TBC increased linearly, and the mean increase in TBC for a given estradiol increment was constant until ...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1987-03-01 00:00:00
abstract：:Prospective HLA-DR matching has been shown to enhance renal graft survival. It has also been demonstrated that the major stimuli in the one-way mixed lymphocyte culture reaction (MLR) are the DR rather than DQ class II histocompatibility antigens. A two antigen DR match between the potential recipient and donor is usu...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1987-09-01 00:00:00
abstract：:The histopathologic findings in lung tissue are reported from five cases of Philadelphia Legionnaire's Disease and the results are compared to pneumonias caused by other microbial and chemical agents. Histopathology of lung tissue was similar in all cases, despite the fact that death occurred between the fourth and 14...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1979-09-01 00:00:00
abstract：:BCR/ABL1 transcripts, the molecular hallmarks of chronic myeloid leukemia (CML), have been detected in peripheral blood from healthy individuals. Although CML is a sporadic disease, familial occurrence has been reported. This raises the question of whether there is a hereditary factor related to the etiology of CML. O...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2019-11-01 00:00:00
abstract：:At the cellular level, mutations in deoxyribonucleic acid (DNA) can lead to synthesis of altered proteins which are unable to sustain specific cell functions, eventually leading to its death. Veritably apoptosis, or programmed cell death, is a device to eliminate heavily mutated cells. Cell membranes with altered prot...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章,评审
doi：
更新日期：1996-01-01 00:00:00
abstract：:The aim of the study was to determine if amniotic fluid cells of rats can be used to provide evidence of genotoxicity. In order to do that micronuclei formation was induced in rats during pregnancy after treatment with cyclophosphamide (CP), at different CP doses. On gestational day 19, we collected the amniotic fluid...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2018-03-01 00:00:00
abstract：:Point-of-care testing (POCT) has been used for illicit substance screening in several settings, primarily in law enforcement and drug treatment centers. In this study, we evaluate the use of this screening approach in the emergency department (ED) of a tertiary-care, urban medical center. Aliquots of urine specimens w...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2002-10-01 00:00:00
abstract：:Spindle cell variant of cutaneous B-cell lymphoma is a rare entity which poses a diagnostic difficulty. The differential diagnosis of cutaneous spindle cell lesions is broad, and often includes sarcoma, carcinoma, and melanoma. Lymphoma with spindle cell morphology is frequently only considered after exclusion of more...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2016-04-01 00:00:00
abstract：:Alterations in ion transport have been implicated in the pathogenesis of several disease states. Methods of studying ion transport, however, are rather tedious, time consuming and not well defined in terms of kinetics and reproducibility. This paper describes a rapid, simple method of examining ion transport in intact...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：1981-03-01 00:00:00
abstract：:We report 2 patients who presented with vasomotor symptoms and severe thrombocytopenia following rattlesnake bites. These symptoms persisted in spite of treatment with antivenin and transfusion of multiple doses of platelets. Thrombocytopenia is a common occurrence in moderate to severe crotaline envenomation. Algorit...
journal_title：Annals of clinical and laboratory science
pub_type： 杂志文章
doi：
更新日期：2004-10-01 00:00:00
