Abstract：
:Organoid techniques provide unique platforms to model brain development and neurological disorders. Whereas several methods for recapitulating corticogenesis have been described, a system modeling human medial ganglionic eminence (MGE) development, a critical ventral brain domain producing cortical interneurons and related lineages, has been lacking until recently. Here, we describe the generation of MGE and cortex-specific organoids from human pluripotent stem cells that recapitulate the development of MGE and cortex domains, respectively. Population and single-cell RNA sequencing (RNA-seq) profiling combined with bulk assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) analyses revealed transcriptional and chromatin accessibility dynamics and lineage relationships during MGE and cortical organoid development. Furthermore, MGE and cortical organoids generated physiologically functional neurons and neuronal networks. Finally, fusing region-specific organoids followed by live imaging enabled analysis of human interneuron migration and integration. Together, our study provides a platform for generating domain-specific brain organoids and modeling human interneuron migration and offers deeper insight into molecular dynamics during human brain development.
journal_name
Cell Stem Celljournal_title
Cell stem cellauthors
Xiang Y,Tanaka Y,Patterson B,Kang YJ,Govindaiah G,Roselaar N,Cakir B,Kim KY,Lombroso AP,Hwang SM,Zhong M,Stanley EG,Elefanty AG,Naegele JR,Lee SH,Weissman SM,Park IHdoi
10.1016/j.stem.2017.07.007subject
Has Abstractpub_date
2017-09-07 00:00:00pages
383-398.e7issue
3eissn
1934-5909issn
1875-9777pii
S1934-5909(17)30286-2journal_volume
21pub_type
杂志文章相关文献
Cell Stem Cell文献大全abstract：:As a response against serious infection, there is often a need to produce more leukocytes to defend the organism against the infectious agent. In this issue of Cell Stem Cell, Goodell and colleagues (Feng et al., 2008) find a link between regulators of host defense and hematopoietic stem cells. ...
journal_title：Cell stem cell
pub_type： 评论,社论
doi：10.1016/j.stem.2007.12.002
更新日期：2008-01-10 00:00:00
abstract：:Centralized banking of human embryonic stem (hES) cells is an endeavor that can benefit individual research efforts and enhance international collaboration but is complicated by the fact that the science is rapidly evolving in an environment of heterogeneous laws, guidelines, and ethical standards. Written from the va...
journal_title：Cell stem cell
pub_type： 杂志文章,评审
doi：10.1016/j.stem.2008.03.018
更新日期：2008-04-10 00:00:00
abstract：:Cells of adult mammals can be converted (reprogrammed) to new cells. In one approach, adult cells are converted to pluripotent stem cells, followed by differentiation to regenerate new cell types. Alternatively, adult cells may be directly converted into other mature cells or progenitors. We discuss and compare these ...
journal_title：Cell stem cell
pub_type： 杂志文章,评审
doi：10.1016/j.stem.2008.09.015
更新日期：2008-10-09 00:00:00
abstract：:The generation of human embryonic stem cell banking networks has ensured that well-characterized and quality controlled stem cell lines are broadly accessible to researchers worldwide. Here, we provide recommendations for engaging these established networks in efforts to build similar resources for the distribution an...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2013.09.007
更新日期：2013-10-03 00:00:00
abstract：:The suspected link between infection by Zika virus (ZIKV), a re-emerging flavivirus, and microcephaly is an urgent global health concern. The direct target cells of ZIKV in the developing human fetus are not clear. Here we show that a strain of the ZIKV, MR766, serially passaged in monkey and mosquito cells efficientl...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2016.02.016
更新日期：2016-05-05 00:00:00
abstract：:Dysfunction and loss of retinal pigment epithelium (RPE) leads to degeneration of photoreceptors in age-related macular degeneration and subtypes of retinitis pigmentosa. Human embryonic stem cells (hESCs) may serve as an unlimited source of RPE cells for transplantation in these blinding conditions. Here we show the ...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2009.07.002
更新日期：2009-10-02 00:00:00
abstract：:The lineage restriction of prospectively isolated hematopoietic progenitors has been traditionally assessed by bulk in vitro culture and transplantation of large number of cells in vivo. These methods, however, cannot distinguish between homogenous multipotent or heterogeneous lineage-restricted populations. Using clo...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2010.02.013
更新日期：2010-04-02 00:00:00
abstract：:In this issue of Cell Stem Cell, Hooper et al. (2009) use a combination of immunohistochemistry and flow cytometry to characterize the bone marrow vasculature both before and after injury. The authors demonstrate that recovery of normal hematopoiesis after myelosuppressive insult is dependent upon endothelial VEGFR2. ...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2009.02.005
更新日期：2009-03-06 00:00:00
abstract：:Understanding the fetal hepatic niche is essential for optimizing the generation of functional hepatocyte-like cells (hepatic cells) from human embryonic stem cells (hESCs). Here, we show that KDR (VEGFR2/FLK-1), previously assumed to be mostly restricted to mesodermal lineages, marks a hESC-derived hepatic progenitor...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2013.04.026
更新日期：2013-06-06 00:00:00
abstract：:Cardiac stem/progenitor cells hold great potential for regenerative therapies; however, the mechanisms regulating their expansion and differentiation remain insufficiently defined. Here we show that Ldb1 is a central regulator of genome organization in cardiac progenitor cells, which is crucial for cardiac lineage dif...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2015.08.007
更新日期：2015-09-03 00:00:00
abstract：:Chronic myeloid leukemia (CML) originates in a hematopoietic stem cell (HSC) transformed by the breakpoint cluster region (BCR)-abelson (ABL) oncogene and is effectively treated with tyrosine kinase inhibitors (TKIs). TKIs do not eliminate disease-propagating leukemic stem cells (LSCs), suggesting a deeper understandi...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2019.02.018
更新日期：2019-05-02 00:00:00
abstract：:Cellular barcoding offers a powerful approach to characterize the growth and differentiation activity of large numbers of cotransplanted stem cells. Here, we describe a lentiviral genomic-barcoding and analysis strategy and its use to compare the clonal outputs of transplants of purified mouse and human basal mammary ...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2013.12.011
更新日期：2014-02-06 00:00:00
abstract：:In response to muscle injury, satellite cells activate the p38α/β MAPK pathway to exit quiescence, then proliferate, repair skeletal muscle, and self-renew, replenishing the quiescent satellite cell pool. Although satellite cells are capable of asymmetric division, the mechanisms regulating satellite cell self-renewal...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2012.05.025
更新日期：2012-10-05 00:00:00
abstract：:Embryonic stem cells (ESCs) are an attractive source for tissue regeneration and repair therapies because they can be differentiated into virtually any cell type in the adult body. However, for this approach to succeed, the transplanted ESCs must survive long enough to generate a therapeutic benefit. A major obstacle ...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2011.01.012
更新日期：2011-03-04 00:00:00
abstract：:Fetal gene therapy using safe and effective viral vectors no longer remains a distant prospect. Recently in Nature Medicine, Massaro et al. (2018) demonstrated that prenatal intracranial injection of a viral vector results in improved neurologic function, raising the intriguing possibility that in utero gene therapy m...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2018.08.010
更新日期：2018-09-06 00:00:00
abstract：:Direct cellular reprogramming provides a powerful platform to study cell plasticity and dissect mechanisms underlying cell fate determination. Here, we report a single-cell transcriptomic study of human cardiac (hiCM) reprogramming that utilizes an analysis pipeline incorporating current data normalization methods, mu...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2019.05.020
更新日期：2019-07-03 00:00:00
abstract：:Two recent papers in Cell Stem Cell by Soliman et al. (2020) and Scott et al. (2019) explore the contributions of mesenchymal progenitor cells to fibrosis in heart and skeletal muscle. They find tissue-dependent roles for a subpopulation of mesenchymal progenitors expressing Hic1 with varied differentiation capacities...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2019.12.010
更新日期：2020-02-06 00:00:00
abstract：:While gene expression dynamics have been extensively cataloged during hematopoietic differentiation in the adult, less is known about transcriptome diversity of human hematopoietic stem cells (HSCs) during development. To characterize transcriptional and post-transcriptional changes in HSCs during development, we leve...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2018.03.012
更新日期：2018-04-05 00:00:00
abstract：:Leukemia stem cells (LSCs) are resistant to current therapies used to treat chronic myeloid leukemia (CML). Abraham et al. (2016) have identified a molecular network critical for CML LSC survival and propose that simultaneously targeting two of their major transcriptional regulators, p53 and c-Myc, may facilitate thei...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2016.06.005
更新日期：2016-07-07 00:00:00
abstract：:Emerging evidence strongly suggests that stem cells and their differentiated progeny display distinct metabolic profiles, but how metabolic changes are coupled with organogenesis has remained unclear. Homem et al. (2014) now reveal a hormone-dependent pathway that couples metabolic changes with stem cell differentiati...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2014.08.006
更新日期：2014-09-04 00:00:00
abstract：:Cerebral organoids represent a new model in which to study human brain development outside the human body. Recently in Nature Biotechnology, Lancaster et al. (2017) tackled the lack of reproducibility, tissue homogeneity, and complexity of this system by bioengineering organoids to establish the next generation of hum...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2017.07.001
更新日期：2017-08-03 00:00:00
abstract：:Animal cloning can be achieved through somatic cell nuclear transfer (SCNT), although the live birth rate is relatively low. Recent studies have identified H3K9me3 in donor cells and abnormal Xist activation as epigenetic barriers that impede SCNT. Here we overcome these barriers using a combination of Xist knockout d...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2018.06.008
更新日期：2018-09-06 00:00:00
abstract：:Several cell populations have been reported to possess intestinal stem cell (ISC) activity during homeostasis and injury-induced regeneration. Here, we explored inter-relationships between putative mouse ISC populations by comparative RNA-sequencing (RNA-seq). The transcriptomes of multiple cycling ISC populations clo...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2017.06.014
更新日期：2017-07-06 00:00:00
abstract：:Cell engineering has brought us tantalizingly close to the goal of deriving patient-specific hematopoietic stem cells (HSCs). While directed differentiation and transcription factor-mediated conversion strategies have generated progenitor cells with multilineage potential, to date, therapy-grade engineered HSCs remain...
journal_title：Cell stem cell
pub_type： 杂志文章,评审
doi：10.1016/j.stem.2016.05.016
更新日期：2016-06-02 00:00:00
abstract：:N6-methyladenosine (m6A), the most abundant internal modification in mRNA, has been implicated in tumorigenesis. As an m6A demethylase, ALKBH5 has been shown to promote the development of breast cancer and brain tumors. However, in acute myeloid leukemia (AML), ALKBH5 was reported to be frequently deleted, implying a ...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2020.04.009
更新日期：2020-07-02 00:00:00
abstract：:Reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) can be achieved by viral-mediated transduction of defined transcription factors. Moving toward the eventual goal of clinical application, it is necessary to overcome limitations such as low reprogramming efficiency and genomic alterations due to ...
journal_title：Cell stem cell
pub_type： 杂志文章,评审
doi：10.1016/j.stem.2009.03.005
更新日期：2009-04-03 00:00:00
abstract：:In this issue of Cell Stem Cell, Wang et al. (2014) describe a signaling axis present in bone marrow stromal cells that suppresses inflammation and myeloid expansions. Loss of endothelial Notch signaling leads to deregulation of miR-155 expression, activation of NF-kB, and increased proinflammatory cytokine production...
journal_title：Cell stem cell
pub_type： 评论,杂志文章
doi：10.1016/j.stem.2014.06.010
更新日期：2014-07-03 00:00:00
abstract：:In injured tissues, regeneration is often associated with cell fate plasticity, in that cells deviate from their normal lineage paths. It is becoming increasingly clear that this plasticity often creates alternative strategies to restore damaged or lost cells. Alternatively, cell fate plasticity is also part and parce...
journal_title：Cell stem cell
pub_type： 杂志文章,评审
doi：10.1016/j.stem.2018.05.009
更新日期：2018-09-06 00:00:00
abstract：:Cancer stem cells (CSCs) are tumor cells that have the principal properties of self-renewal, clonal tumor initiation capacity, and clonal long-term repopulation potential. CSCs reside in niches, which are anatomically distinct regions within the tumor microenvironment. These niches maintain the principle properties of...
journal_title：Cell stem cell
pub_type： 杂志文章,评审
doi：10.1016/j.stem.2015.02.015
更新日期：2015-03-05 00:00:00
abstract：:Pluripotent stem cells (PSCs) could provide a powerful system to model development of the human esophagus, whose distinct tissue organization compared to rodent esophagus suggests that developmental mechanisms may not be conserved between species. We therefore established an efficient protocol for generating esophagea...
journal_title：Cell stem cell
pub_type： 杂志文章
doi：10.1016/j.stem.2018.08.009
更新日期：2018-10-04 00:00:00
