Abstract:
BACKGROUND:Umbilical cord blood (UCB) processing with hydroxyethyl starch (HES) is the most common protocol in the cord blood banks. The quality of UCB volume reduction was guaranteed by minimum manipulation of cord blood samples in the closed system. This study aimed to analyze and compare cell recovery and viability of UCB processed using the Sepax automated system in the presence and absence of HES. STUDY DESIGN AND METHODS:Thirty UCB bags with a total nucleated cell (TNC) count of more than 2.5 × 109 were divided in two bags with equal volume. HES solution was added to one bag and another was intact. Both bags were processed with the Sepax. To determine cell recovery, viability, and potential of colony-forming cells (CFCs), preprocessing, postprocessing, and thawing samples were analyzed. RESULTS:The mean TNC recovery after processing and after thaw was significantly better with the HES method (p < 0.01 for the postprocessing step and p < 0.05 for the postthaw step). There were no significant differences to mononucleated cells (MNCs) and CD34+ cell recovery between the two methods after processing and after thaw. TNC and MNC viability was significantly higher without HES after processing and after thaw (p < 0.01). The results of the CFC assay were similar for both methods after processing and after thaw. CONCLUSION:These results showed that processing of UCB using the Sepax system with the without-HES protocol due to the lower manipulation of samples could be used as an eligible protocol to reduce the volume of UCB.
journal_name
Transfusionjournal_title
Transfusionauthors
Souri M,Nikougoftar Zarif M,Rasouli M,Golzadeh K,Nakhlestani Hagh M,Ezzati N,Atarodi Kdoi
10.1111/trf.14290subject
Has Abstractpub_date
2017-11-01 00:00:00pages
2758-2766issue
11eissn
0041-1132issn
1537-2995journal_volume
57pub_type
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