Abstract:
:Breast cancer is the second leading cause of cancer-associated mortality in females in the USA. Hsa-miR-599 was demonstrated to function as a tumour suppressor during cancer progression. However, the function and mechanism of the hsa-miR-599 in human breast cancer remain elusive. Thus, the aim of the present study was to investigate the potential role of hsa-miR-599 in breast cancer biology. The expression levels of hsa-miR-599 in 40 pairs of surgical specimens and human breast cancer cell lines were detected using quantitative polymerase chain reaction analysis. The overexpression of hsa-miR-599 was established by transfecting mimics into the MCF-7 and MDA-MB-231 cell lines. Cell counting kit-8, colony formation and transwell assays were used to investigate the potential function of hsa-miR-599 in MCF-7 and MDA-MB-231 cell lines. Luciferase assays combined with western blot analysis was performed to validate the regulation of a putative target of hsa-miR-599. The results demonstrated that hsa-miR-599 was downregulated in the breast cancer tissues and breast cancer cell lines. Overexpression of hsa-miR-599 was revealed to inhibit the viability and proliferation of cell in vitro and tumour growth in vivo. The results of the luciferase assay indicate that bromodomain containing 4 (BRD4) is a direct target of hsa-miR-599. Furthermore, the xenograft mouse model demonstrated that overexpressed hsa-miR-599 reduced BRD4 expression. These results suggest that hsa-miR-599 serves as an oncosuppressive microRNA that impairs breast cancer tumorigenesis and progression by directly targeting BRD4. Furthermore, increased BRD4 expression partially reversed the suppressive effect of hsa-miR-599. In conclusion, the results of the present study demonstrated that hsa-miR-599 suppressed breast cancer progression by downregulating BRD4. The overexpression of hsa-miR-599 may be considered as a novel therapeutic target for the treatment of patients with breast cancer.
journal_name
Oncol Lettjournal_title
Oncology lettersauthors
Wang Y,Sui Y,Zhu Q,Sui Xdoi
10.3892/ol.2017.6651subject
Has Abstractpub_date
2017-09-01 00:00:00pages
3455-3462issue
3eissn
1792-1074issn
1792-1082pii
OL-0-0-6651journal_volume
14pub_type
杂志文章相关文献
Oncology Letters文献大全abstract::Value of MRI diffusion-weighted imaging (MRI DWI) combined with PET/CT in the diagnosis and staging of stomach cancer (SC) was investigated. A retrospective analysis was performed on 160 patients with SC diagnosed by pathological biopsy in The Affiliated Yantai Yuhuangding Hospital of Qingdao University from March 201...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10286
更新日期:2019-07-01 00:00:00
abstract::The aim of the present study was to investigate the association between the expression of chemokine receptors CCR7 and CXCR4 and vascular endothelial growth factor (VEGF)-C and the lymph node metastasis of pancreatic ductal adenocarcinoma (PDAC). The mRNA transcription levels of CCR7, CXCR4 and VEGF-C were measured in...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1261
更新日期:2013-05-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is one of the leading digestive malignancies, with a high metastasis and recurrence. The development and rapid progression of HCC involves numerous complex molecular and cellular events. Therefore, developing effective methods for the prevention and treatment of HCC requires an improved ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6369
更新日期:2017-08-01 00:00:00
abstract::The aim of the current study was to investigate the effects of resveratrol (Res) on vascular endothelial growth factor (VEGF) expression and cell proliferation in the human osteosarcoma cell line U20S. U20S cells were treated with Res at various concentrations (0, 10, 20 and 40 μmol/l) for various times (24, 48 and 72...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.824
更新日期:2012-10-01 00:00:00
abstract::Esophageal cancer is one of the most common types of malignant tumors located within the digestive system, with >50% of esophageal cancer cases worldwide occurring in China. Recent studies have demonstrated that long non-coding RNAs (lncRNAs) are frequently dysregulated in cancer; however, few lncRNAs have been charac...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.7968
更新日期:2018-04-01 00:00:00
abstract::Corosolic acid (CRA), a pentacyclic triterpene isolated from medicinal herbs, has been reported to exhibit anticancer properties in several cancers. However, the anticancer activity of CRA in osteosarcoma cells is still unclear. In the present study, the inhibitory effect of CRA in osteosarcoma MG-63 cells was investi...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.5185
更新日期:2016-11-01 00:00:00
abstract::Sclerosing stromal tumor (SST) of the ovary, which was first described by Chalvardjian and Scully in 1973, is a rare ovarian neoplasm, occurring predominantly in young women. The most common clinical symptom in patients with SST is menstrual irregularities. Microscopically, the tumor is characterized by the presence o...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4441
更新日期:2016-06-01 00:00:00
abstract::Mosquito coils, which are commonly used as residential insecticides in Asia, contain different concentrations of octachlorodipropyl ether (S-2) as a synergist or an active ingredient. As bis(chloromethyl) ether (BCME) is an extremely potent lung carcinogen that can be produced by the thermolytic degradation of S-2, co...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.2922
更新日期:2015-04-01 00:00:00
abstract::It is estimated that breast cancer will be the second leading cause of cancer-associated mortality in women in 2018. Previous research has demonstrated that the atypical protein kinase C-ζ (PKC-ζ) is a component of numerous dysregulated pathways in breast cancer, including cellular proliferation, survival, and cell cy...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9792
更新日期:2019-02-01 00:00:00
abstract::The ubiquitin-proteasome pathway is an important protein degradation regulatory system in cells. This pathway is also a reversible process that is strictly regulated, and the regulation of deubiquitinating enzymes (DUBs) represents an important facet of the process. Ovarian tumor-associated proteases domain-containing...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10883
更新日期:2019-11-01 00:00:00
abstract::Primary penile lymphoma presenting with priapism as the initial symptom is extremely rare. In total, <10 cases have been previously reported. The diagnosis can be difficult and patients often develop metastasis. The current study reports the case of a 48-year-old male, who presented with a one-month history of painles...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2488
更新日期:2014-11-01 00:00:00
abstract::Radiation enterocolitis is a severe adverse event that occurs after radiotherapy for malignant abdominal tumors. In this study, the therapeutic effects of ETS-GS, a novel vitamin E derivative with antioxidative and anti-inflammatory abilities, were examined in a rat model of radiation enterocolitis. The radiation ente...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1581
更新日期:2013-11-01 00:00:00
abstract::Brain-specific metastasis is one of the primary causes of recurrence following complete resection of non-small cell lung cancer (NSCLC) and the underlying mechanism remains unclear. The present study was designed to investigate the correlation between C-X-C chemokine receptor type 4 (CXCR4) expression and brain-specif...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.1979
更新日期:2014-06-01 00:00:00
abstract::Salidroside has a wide range of pharmacological activities, including antitumor, anti-inflammatory, analgesic, antibacterial, antiviral and anti-fertility abilities. In the present study, the effects of salidroside on the viability and apoptosis of bladder cancer cells, and the potential underlying mechanisms, were ex...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8982
更新日期:2018-09-01 00:00:00
abstract::2',4'-dihydroxy-6'-methoxychalcone (cardamonin) is a natural compound with anti-proliferative effects on several cancer types including nasopharyngeal carcinoma. The effects of cardamonin on melanoma cells are unknown. The present study investigated the anti-proliferative effect of cardamonin on human melanoma cell li...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.11242
更新日期:2020-02-01 00:00:00
abstract::Microglia, once assimilated to peripheral macrophages, in gliomas has long been discussed and currently it is hypothesized to play a pro-tumor role in tumor progression. Uncertain between M1 and M2 polarization, it exchanges signals with glioma cells to create an immunosuppressive microenvironment and stimulates cell ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7386
更新日期:2018-01-01 00:00:00
abstract::Although there is evidence that galectin-9 is a critical factor in health and disease, the upstream regulatory microRNA (miRNA or miR) of the protein remains poorly defined. miR-455-5p is characterized as a tumor-associated miRNA in cancer research. However, the actual role of miR-455-5p with respect to inhibiting or ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.5608
更新日期:2017-03-01 00:00:00
abstract::MicroRNAs (miRNAs) are small non-coding RNAs of approximately 22 base pairs that regulate the expression of genes by targeting messenger RNA with complementarity with the miRNA base sequence. Regulation of gene expression by miRNAs is crucial in cellular development and differentiation, and recent studies suggest a re...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2010.173
更新日期:2010-11-01 00:00:00
abstract::It has been demonstrated that numerous types of metastatic cancer overexpress vacuolar-type H+ (V)-ATPases. It may be possible to inhibit the growth and metastasis of human cancer cells by inhibiting V-ATPases. It was previously reported that diphyllin, a novel V-ATPase inhibitor, can inhibit the migration and invasio...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6917
更新日期:2017-11-01 00:00:00
abstract::For osteoblastoma, with its predilection for the spinal column and appendicular skeleton, the skull is an unusual site, and paranasal sinus involvement is very rare. Herein, we report on a case in which the disease was located within the sphenoid bone. To the best of our knowledge, this is the 4th reported case of ost...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol_00000065
更新日期:2010-03-01 00:00:00
abstract::Cerebroma, a nervous system tumor located in the cranial cavity, seriously affects health and may even become life-threatening. The aim of the study was to investigate and discuss the expression levels of cluster of differentiation 44 (CD44) and Golgi protein 73 (GP73) in different types of cerebroma tissues and to st...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.7904
更新日期:2018-04-01 00:00:00
abstract::Breast cancer is a common malignant tumor among females, with triple-negative breast cancer being an important type accounting for 15-20% of all breast cancer cases. Triple-negative breast cancer is one of the most aggressive types of cancer without standard adjuvant chemotherapy. Ganoderic acid A (GA-A) is one of the...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9475
更新日期:2018-11-01 00:00:00
abstract::In the present study, we investigated the correlation between the presence of occult neoplastic cells (ONCs) in lymph node sinuses and recurrence/metastasis of stage II/III gastric cancer in 164 patients who underwent radical curative resection. We calculated the five-year relapse-free survival rate (5Y-RFS) and five-...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1660
更新日期:2014-01-01 00:00:00
abstract::The aim of the present study was to assess the expression of microRNA-146a (miR-146a) in human lung adenocarcinoma cells, its effect on cellular behaviors, and the underlying molecular mechanisms. Reverse transcription-quantitative PCR (RT-qPCR) was used to measure miR-146a expression in the human normal lung epitheli...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.11474
更新日期:2020-06-01 00:00:00
abstract::Chronic myelogenous leukemia (CML) is a common hematological malignancy. Some patients progressing to the blast phase develop chemotherapeutic drug resistance. In the authors' previous study, it was found that the mammalian target of rapamycin (mTOR) pathway was activated in CML and that rapamycin inhibited the prolif...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10589
更新日期:2019-09-01 00:00:00
abstract::Colorectal cancer (CRC) is the third leading cause of cancer-associated mortalities. Long non-coding RNAs (lncRNAs) have been identified as key regulators in the occurrence and development of CRC. The lncRNA urothelial cancer associated 1 (UCA1) has been demonstrated to promote the development of numerous different ty...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10737
更新日期:2019-10-01 00:00:00
abstract::Evaluation of carcinogenic substances from the environment is a challenge for scientists. Recently, a novel approach based on 10 key characteristics of human carcinogens classified by the International Agency for Research on Cancer (IARC) has emerged. Carcinogenesis depends on different mechanisms and factors, includi...
journal_title:Oncology letters
pub_type: 杂志文章,评审
doi:10.3892/ol.2020.11566
更新日期:2020-07-01 00:00:00
abstract::Long non-coding RNA (lncRNA) has been implicated in various types of human cancer. However, the role of lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in gastric cancer remains unclear. In the present study, MALAT1 expression was significantly upregulated in gastric tumors compared with adjacen...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10253
更新日期:2019-06-01 00:00:00
abstract::Carbon dioxide (CO2) laser ablation in combination with photodynamic therapy (PDT) has previously been successfully used to treat superficial basal cell carcinoma (BCC). However, the efficacy of this treatment modality is limited in the treatment of deeper lesions and the more aggressive subtypes of BCC. In order to i...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1504
更新日期:2013-10-01 00:00:00
abstract::Exophthalmos, myxedema and osteoarthropathy (EMO) comprise the triad known as EMO syndrome, which is rarely observed in patients with autoimmune thyroid disease. The present study reports the case of a patient with EMO, including the response of this rare combination to radiotherapy. A 48-year-old patient with EMO syn...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.2990
更新日期:2015-05-01 00:00:00