Glycoproteins and glycolipids of rat small intestinal microvillus and basolateral membranes.

Abstract:

:Glycoprotein and glycolipid constituents were examined in purified microvillus and basolateral membranes isolated from rat small intestinal epithelial cells. SDS-polyacrylamide gel electrophoresis showed that the molecular weights of most of the major proteins from microvillus membranes were over 100 kD, whereas the majority of those from basolateral membranes tended to have lower molecular weights. Glycoprotein profiles were also examined using three labeling methods, and in each case marked differences were observed between microvillus and basolateral membranes. In both membranes, lectins with a specificity toward N-linked sugar chains bound to the majority of the glycoproteins, in contrast to those lectins which preferentially bind to O-linked sugar chains. Glycolipids were labeled in vivo and isolated from both membrane fractions. Some differences were observed in the fucolipids and neutral glycolipids suggesting a more complex pattern in microvillus membranes. These results indicate that there are differences in the glycoprotein and glycolipid compositions of microvillus and basolateral membranes that may reflect the functional polarity of intestinal epithelial cells.

journal_name

Dig Dis Sci

authors

Morita A,Siddiqui B,Erickson RH,Kim YS

doi

10.1007/BF01536338

subject

Has Abstract

pub_date

1989-04-01 00:00:00

pages

596-605

issue

4

eissn

0163-2116

issn

1573-2568

journal_volume

34

pub_type

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