Abstract:
:Anterior cruciate ligament injuries are common in humans, though cellular components of the knee have little regenerative or proliferation potential. This study investigated the differentiation of human amnion-derived mesenchymal stem cells (hAMSCs) into human anterior cruciate ligament fibroblasts (hACLFs) in vitro through induction with bFGF and TGF-β1 with coculture systems. Groups A and B comprised hAMSCs at the 3rd passage cultured with and without bFGF and TGF-β1, respectively; Groups C and D consisted of hAMSCs and hACLFs in monolayer coculture with and without bFGF and TGF-β1, respectively; Groups E and F were composed of hAMSCs and hACLFs in Transwell coculture with and without bFGF and TGF-β1, respectively. Cell morphology and proliferation were recorded. Protein expression and relative mRNA expression were evaluated in each group. Cell proliferation was significantly higher in the induced groups than in the noninduced groups. Protein expression increased over time with the highest expression observed in Group E. mRNA levels were significantly higher in Group E than in other groups. This study is the first to demonstrate the use of the Transwell coculture system for this purpose, and hAMSCs were successfully differentiated into hACLFs. Thus, hAMSCs may be a superior choice for hACLF differentiation via Transwell coculture.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Li Y,Liu Z,Jin Y,Zhu X,Wang S,Yang J,Ren Y,Fu Q,Xiong H,Zou G,Liu Ydoi
10.1155/2017/7360354subject
Has Abstractpub_date
2017-01-01 00:00:00pages
7360354eissn
2314-6133issn
2314-6141journal_volume
2017pub_type
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/1507979
更新日期:2018-09-27 00:00:00
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doi:10.1155/2016/8748023
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doi:10.1155/2020/8718097
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doi:10.1155/2013/637424
更新日期:2013-01-01 00:00:00
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doi:10.1155/2020/8506365
更新日期:2020-08-17 00:00:00
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doi:10.1155/2013/283856
更新日期:2013-01-01 00:00:00
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doi:10.1155/2019/4093214
更新日期:2019-12-14 00:00:00
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doi:10.1155/2016/5184595
更新日期:2016-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2014/204532
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doi:10.1155/2016/6492183
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pub_type: 临床试验,杂志文章
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pub_type: 杂志文章
doi:10.1155/2013/201475
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pub_type: 杂志文章,评审
doi:10.1155/2014/403120
更新日期:2014-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/2759035
更新日期:2017-01-01 00:00:00