Abstract:
:Cellular distribution of progestin receptor immunoreactivity (PR-IR) in paraffin-embedded rabbit oviduct tissue was determined using a monoclonal antibody and the indirect immunoperoxidase method. Ampullar and isthmic tissue from ovariectomized animals primed with 17 beta-estradiol or vehicle alone was examined. As expected, in the absence of estradiol, PR-IR was rarely observed in the ampulla. Estradiol treatment increased PR-IR in cell nuclei of the stroma and muscularis of the ampulla, but not of the epithelium. In contrast, PR-IR was seen in epithelial cell nuclei of the isthmus in the absence of estradiol. Surprisingly, while estradiol treatment increased PR-IR in cell nuclei of the stroma and muscularis of the isthmus, it decreased PR-IR in the epithelium. These results suggest for the first time a differential regulation of progestin receptors in the ampulla and isthmus of the oviduct. In particular, while estradiol treatment increased PR-IR in the stroma and muscularis and was without effect on PR-IR in the epithelium of the ampulla, it decreased PR-IR in the epithelium of the isthmus.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Hyde BA,Blaustein JD,Black DLdoi
10.1210/endo-125-3-1479subject
Has Abstractpub_date
1989-09-01 00:00:00pages
1479-83issue
3eissn
0013-7227issn
1945-7170journal_volume
125pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Atrial Natriuretic Factor (ANF) has been demonstrated within the central nervous system (CNS), where it has been implicated in the regulation of blood pressure, fluid and electrolyte balance. To explore the effect of acute plasma vol expansion on the activation of CNS ANF, changes in ANF levels of CSF drawn from the c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-5-2587
更新日期:1990-11-01 00:00:00
abstract::Recent research progress indicates a close link between ghrelin, a natural ligand of GH secretagogues receptor (GHS-R), and both the metabolic balance and body composition. To clarify the involvement of ghrelin and GHS-R in the process of adipogenesis, we measured the expression of GHS-R and peroxisome proliferator-ac...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220783
更新日期:2003-03-01 00:00:00
abstract::We have examined the effects of acute administration of the cannabinoid receptor type 1 (CB(1)) antagonist AM251 on the rat hypothalamic-pituitary-adrenal (HPA) axis with respect to both gender and time of day. Blood samples were collected from conscious male and female rats every 5 min using an automated blood sampli...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0101
更新日期:2010-08-01 00:00:00
abstract::The quantities of estrone and estradiol were determined by radioimmunoassay in maternal and fetal plasma of the rhesus monkey from day 59 to 163 of gestation. A two way analysis of variance of data classified according to fetal sex and 3 pooled gestational ages for each hormone and for mother or fetus (4 analyses) rev...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-2-425
更新日期:1975-08-01 00:00:00
abstract::Immunohistochemical (immunoperoxidase) studies were performed on 478 sections from 97 rat pituitary glands with rabbit antisera to unconjugated human synthetic calcitonin beta-endorphin, and/or ACTH-(17--39). Calcitonin-positive cells were present in a majority of the anterior lobes studied, whereas they were present ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-106-6-1966
更新日期:1980-06-01 00:00:00
abstract::To further characterize the effect of TSH administration on thyroid iodothyronine monodeiodinating activity, we have evaluated the in vitro conversion of T4 to T3 (outer ring deiodination) and T3 to 3,3'-diiodothyronine (T2; inner ring deiodination) by mouse thyroid, liver, and kidney homogenates, comparing tissues fr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-3-901
更新日期:1985-03-01 00:00:00
abstract::Whole saliva collected from rat, mouse, and human sources was found to contain high concentrations of transforming growth factor-alpha (TGF alpha) when analyzed by RIA. The concentrations of TGF alpha in unstimulated human saliva (age, 30-45 yr; n = 10; 1.5 +/- 3.1 nM) was reduced with age (age, 55-70 yr; n = 10; 0.4 ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.2.8299590
更新日期:1994-02-01 00:00:00
abstract::Wortmannin (5-100 nM), a specific phosphatidyinositol 3-kinase inhibitor, augmented 8 mM glucose-induced insulin secretion from control Sprague Dawley rat islets in a dose-dependent manner. This effect persisted after its removal from the perifusion medium; however, this augmenting effect was reduced by the calcium ch...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.9.7636
更新日期:2000-09-01 00:00:00
abstract::The conceptus and endometrium secrete large amounts of prostaglandin E₂ (PGE₂) into the porcine uterine lumen during the periimplantation period. We hypothesized that PGE₂ acts on conceptus/trophoblast cells through auto- and paracrine mechanisms. Real-time RT-PCR analysis revealed that PGE₂ receptor (PTGER)2 mRNA was...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2271
更新日期:2013-10-01 00:00:00
abstract::Since hypophysectomy and GH deficiency are associated with decreases in hypothalamic content and release of SRIF, it was of interest to determine whether these hormonal alterations also affect peripheral tissue levels of SRIF. Hypophysectomized (hypox) rats were studied at various times after surgery and compared with...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-6-2260
更新日期:1984-12-01 00:00:00
abstract::Although the pituitary-grafted rat is a classic model of chronic PRL excess, the presence of somatotropes in grafted pituitary tissue indicates a potential for GH secretion. The current study was designed to investigate GH-releasing hormone (GRH)-induced GH secretion and beta-adrenergic inhibition of GH release in ani...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-5-2273
更新日期:1989-11-01 00:00:00
abstract::Sertoli cell intracellular protein 1 (SCc1) and 2 (SCc2) are polypeptides found in rat Sertoli cell cultures incubated with either FSH or (Bu)2cAMP. They were first identified in [35S]methionine-labeled Sertoli cell lysates using two-dimensional gel electrophoresis. Here we extend these observations by showing that SC...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.5.1330490
更新日期:1992-11-01 00:00:00
abstract::Telomerase activity is essential for protection of cells against the telomere erosion that occurs with each round of cell replication, and thus appears to play a role in the indefinite replication potential of some, but not all, eukaryotic cells. In this regard, some tissues contain stem cells that have a long prolife...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.3.5839
更新日期:1998-03-01 00:00:00
abstract::Two surges of PRL designated as nocturnal (N) and diurnal (D) are observed during early pregnancy. The present study was undertaken to determine the involvement of serotonin (5-HT) in regulating these PRL surges. On day 7 of pregnancy intracarotid catheters were implanted, and blood samples were obtained at 0200 and 0...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-6-2875
更新日期:1989-12-01 00:00:00
abstract::Maintenance of cell polarity is essential for Sertoli cell and blood-testis barrier (BTB) function and spermatogenesis; however, the signaling mechanisms that regulate the integrity of the cytoskeleton and polarity of Sertoli cells are not fully understood. Here, we demonstrate that rapamycin-insensitive component of ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1217
更新日期:2015-11-01 00:00:00
abstract::Insulin-like growth factor-I (IGF-I), the principal IGF in adult rat serum, occurs complexed to specific binding proteins. After fractionation of serum on Sephadex G-200 at neutral pH, 62% of the immunoreactive IGF-I is recovered in the 150K region, 38% in the 40K region, and none is present as free 7.5K IGF-I. Adult ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-3-1540
更新日期:1989-09-01 00:00:00
abstract::The hypothalamic hormone, GnRH, is released and transported to the anterior pituitary in a pulsatile manner, where it binds to specific high-affinity receptors and regulates gonadotropin biosynthesis and secretion. The frequency of GnRH pulses changes under various physiological conditions, and varying GnRH pulse freq...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.3.4968
更新日期:1997-03-01 00:00:00
abstract::The distribution pattern of messenger RNAs (mRNAs) for insulin-like growth factor I (IGF-I), IGF-II, and the type 1 IGF receptor and the detection of IGF-binding sites in sections of ovine ovary were demonstrated using in situ hybridization and autoradiography. Ovaries were collected from 30 ewes at time points throug...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.12.7588270
更新日期:1995-12-01 00:00:00
abstract::The present study investigates the effect of different endocrine manipulations on the gamma-aminobutyric acid (GABA)-ergic system in the rat fallopian tube. Either hypophysectomy or ovariectomy induced a significant decrease of glutamic acid decarboxylase (GAD) activity and of GABA levels in in situ tubes. This effect...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-1-334
更新日期:1986-01-01 00:00:00
abstract::To determine the relative role of the androgenic and/or estrogenic components of the action of dehydroepiandrosterone (DHEA) on the histomorphology and structure of the rat mammary gland, ovariectomized (OVX) female animals received DHEA administered alone or in combination with the pure antiandrogen flutamide or the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.2.5762
更新日期:1998-02-01 00:00:00
abstract::Estrogen inhibits postmenopausal bone loss and decreases fracture risk. Unfortunately, estrogen replacement therapy has many undesirable side effects, the majority of which are due to stimulation of reproductive tissues. Tissue specific estrogen agonists provide a promising new alternative to natural estrogens for hor...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.5.5109
更新日期:1997-05-01 00:00:00
abstract::To elucidate the mechanism of humoral hypercalcemia elicited by human esophageal carcinoma cells (EC-GI), which constitutively produced interleukin-1 alpha (IL-1 alpha) and PTH-like factor, the effects of IL-1 alpha and PTH-related protein (PTH-rP) on bone resorption in vitro and on serum calcium concentrations in viv...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-5-2172
更新日期:1989-05-01 00:00:00
abstract::Insulin radioreceptor assays were developed using splenic leukocytes and peripheral erythrocytes from individual mice. Splenic leukocytes were prepared using an NH4Cl buffer which did not alter insulin binding, but gave much higher yields than density gradient methods. Mouse erythrocytes were isolated from heparinized...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-110-2-474
更新日期:1982-02-01 00:00:00
abstract::We are investigating the mechanisms by which aromatic hydrocarbons, such as 3-methylcholanthrene (MC), suppress hepatic cytochrome P450 2C11 (CYP2C11) gene expression. CYP2C11 is an enzyme expressed in the liver of male rats and is regulated by a pulsatile pattern of GH secretion. We have previously shown that MC atte...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220212
更新日期:2002-09-01 00:00:00
abstract::Three different experimental approaches were used to assess the regions on the beta-subunit of human FSH (hFSH beta) that may be altered or masked by its association with the alpha-subunit of hFSH (hFSH alpha) in the heterodimeric hFSH molecule. In a direct approach, we tested whether synthetic peptides corresponding ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-4-1797
更新日期:1991-04-01 00:00:00
abstract::Immune activation results in the activation of adrenal steroidogenesis and inhibition of gonadal steroidogenesis. Previous studies indicated that these effects were caused primarily by activation and suppression of the secretion of ACTH and LH, respectively. However, other evidence indicated a direct effect of the imm...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.11.7780
更新日期:2000-11-01 00:00:00
abstract::Hypothalamic-pituitary-thyroid (HPT) axis activity is important for energy homeostasis, and is modified by stress. Maternal separation (MS) alters the stress response and predisposes to metabolic disturbances in the adult. We therefore studied the effect of MS on adult HPT axis activity. Wistar male and female pups we...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1239
更新日期:2016-08-01 00:00:00
abstract::The largest subtype of breast cancer is characterized by the expression and activity of the estrogen receptor alpha (ERalpha/ER). Although several effective therapies have significantly improved survival, the adaptability of cancer cells means that patients frequently stop responding or develop resistance to endocrine...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa224
更新日期:2021-02-01 00:00:00
abstract::An isolated perfused rabbit ovary preparation was used to determine the effects of cyanoketone, a potent inhibitor of 3 beta-hydroxysteroid dehydrogenase, on ovulation, ovum maturation and fertilizability, and steroid production. In the first experiment, cyanoketone (10(-4) M) was added to the perfusate of one ovary. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-6-2555
更新日期:1987-06-01 00:00:00
abstract::Human osteoblast-like cells (HOB) produce vascular endothelial growth factor (VEGF), the steady state level of which is stimulated by 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. As osteoblasts and endothelial cells are proximally located in skeletal tissue, we investigated the anabolic effects of 1,25-(OH)2D3 and VEGF on...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.7.5275
更新日期:1997-07-01 00:00:00