Abstract:
:Cytotoxic efficacy of anticancer drugs has been widely studied with monolayer-cultured cancer cells. However, the drug efficacy under two-dimensional (2D) culture conditions usually differs from that of three-dimensional (3D) culture conditions. In the present study, an in vitro tumor tissue model was constructed by sandwiching a cancer cell monolayer (cancer cell sheet) between two collagen layers as a biomimicking tumor tissue model, and the in vitro cytotoxic efficacy of doxorubicin was investigated. In the biomimicking tumor tissue model, hypoxic areas were observed, and the secretion of vascular endothelial growth factor increased time-dependently. Additionally, cell growth inside the model was significantly slower than that of the 2D culture system. The in vitro cytotoxicity of doxorubicin with the 3D system decreased compared to the 2D system, suggesting that the surrounding collagen layer acted as a barrier much like cancer stroma does. Consequently, this study successfully demonstrated that the cancer cell sheet in collagen sandwich configuration provides a useful in vitro tumor tissue model.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Iwase Y,Nakayama M,Yamato M,Okano Tsubject
Has Abstractpub_date
2015-12-01 00:00:00pages
6481-6issue
12eissn
0250-7005issn
1791-7530pii
35/12/6481journal_volume
35pub_type
杂志文章abstract::Glucose consumption and tritiated thymidine incorporation were monitored for 54 days in histocultured human superficial transitional cell carcinoma of the bladder, indicating that long-term histoculture of human transitional cell carcinoma of the bladder is feasible. The glucose-consumption rate was relatively similar...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
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更新日期:2008-07-01 00:00:00
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更新日期:2004-05-01 00:00:00
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更新日期:1998-03-01 00:00:00
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更新日期:2009-06-01 00:00:00
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更新日期:2017-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1983-03-01 00:00:00
abstract::The distribution of receptors for estradiol (ER), progesterone (PgR) and androgens (DHTR) in 104 primary and 49 metastatic breast tumours from Indian women is herein being reported for the first time. When compared to reports in the literature, a lower percentage (47%) of ER (+) tumours and a higher percentage (49%) o...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-07-01 00:00:00
abstract::Multidrug resistance (MDR) is a major clinical obstacle in the treatment of several cancers including hematological malignancies and solid tumors. The ATP-binding cassette transporter B1 (ABCB1) gene and its product, P-glycoprotein (P-gp), is one molecule that is involved in drug resistance. Here we report on the effe...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-10-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1988-03-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12419
更新日期:2018-03-01 00:00:00
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journal_title:Anticancer research
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doi:
更新日期:2015-01-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-08-01 00:00:00
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journal_title:Anticancer research
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doi:
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journal_title:Anticancer research
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更新日期:2020-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
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journal_title:Anticancer research
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doi:
更新日期:2006-03-01 00:00:00
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journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:2007-07-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2004-03-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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