Abstract：
BACKGROUND:Recent data indicate the implication of an IGF/IGFBP/protease system in the development of ectopic implants of endometrium (endometriosis) in the pelvic cavity. MATERIAL AND METHODS:We characterized mitogens and protease(s) against IGFBP-3 in the peritoneal fluid of women with or without endometriosis using preparative isoelectric focusing (IEC) Proteineous fractions were tested for a) mitogenic activity on NIH/3T3 cells and KLE endometrial-like cells, b) proteinolytic activity against radiolabeled IGFBP-3, and c) protein analysis using SDS-PAGE. RESULTS:A significant increase in the DNA content of cultured cell lines was found in PF fractions having slightly acidic or alkaline pH and containing mainly proteins with molecular weights between 50 and 25 kD. These fractions were also found to cleave IGFBP-3 into 25, 18 and 14 kD fragments. Both activities were present in the PF of subjects with or without endometriosis. CONCLUSION:The preparative isoelectric focusing system would be an advantageous approach to investigate the possible implication of the PF proteases with IGFBP-3 limited proteolysis generating PF mitogen(s) which possibly participate in the pathophysiology of endometriosis.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Koutsilieris M,Lavergne E,Lemay Asubject
Has Abstractpub_date
1997-03-01 00:00:00pages
1239-44issue
2Beissn
0250-7005issn
1791-7530journal_volume
17pub_type
杂志文章abstract：:Metastasis is a complex process which results in the growth of tumor cells at sites distant from the primary neoplasm. It is likely that many of the large number of biological changes associated with metastatic ability are controlled through alterations in the expression of a relatively small number of key genes usual...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1990-09-01 00:00:00
abstract：UNLABELLED:Since Rho-mediated signaling can regulate liver cancer cell proliferation, amino acid sequence changes of its downstream targets, actins, might alter the properties of cell growth. Here, we investigated the function of a novel class of actins, named κ-actins, in hepatocellular carcinoma (HCC). MATERIALS AND...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-06-01 00:00:00
abstract：:Adrenocortical carcinoma (ACC) is a rare neoplasm with a poor prognosis. Prognostic factors are needed to identify patients who should be treated aggressively and those for which a less aggressive approach is warranted. As a result of advances within the field of immunohistochemistry, investigations of Ki-67, PCNA, IG...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
abstract：:Two monoclonal antibodies, KM227 (IgM) and KM432 (IgG1), were established by immunizing mice with the crude membrane fraction of lung squamous cell carcinoma and the high-molecular-weight antigen in pleural perfusion from patients with lung adenocarcinoma, respectively. They were found to recognize the carbohydrate ep...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-05-01 00:00:00
abstract：:The polysaccharide produced by the red microalga Porphyridium sp. was highly inhibitory for cell transformation of mouse fibroblast cells by an MSV-124 virus stock. This inhibition was most effective if the polysaccharide was added 2 h before or at the time of infection. The finding that the inhibition of cell transfo...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-05-01 00:00:00
abstract：BACKGROUND:For eradicating portal venous tumor extension and intrahepatic metastasis in hepatocellular carcinoma (HCC), anatomical resection is, in theory, preferable. PATIENTS AND METHODS:We carried-out a retrospective cohort study in 110 patients who underwent curative hepatic resection (anatomical resection; n=20, ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-08-01 00:00:00
abstract：BACKGROUND/AIM:Elderly patients with HPV+ oropharyngeal cancer (OPC) represent an understudied cohort of the HPV epidemic. We aimed to investigate the clinical presentation, treatment tolerability and outcomes in patient's ≥65 years old with HPV+ OPC. PATIENTS AND METHODS:We identified all patients aged 65 and older t...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11520
更新日期：2017-04-01 00:00:00
abstract：PURPOSE:The aim of this study was to determine the relationship between survival and value of molecular markers in the primary tumour in a group of patients with irresectable rectal cancer, treated with preoperative chemo-radiotherapy. MATERIALS AND METHODS:Immunohistochemistry for p53, p21, bcl-2 and Ki-67 was perfor...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：BACKGROUND/AIM:Patients with metastatic head-and-neck cancer require individual therapies facilitated by prognostic tools. A tool to estimate the risk of recurrent or new cerebral metastases following whole-brain irradiation (WBI) is presented. PATIENTS AND METHODS:Age, gender, performance status, cancer site, number ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12714
更新日期：2018-07-01 00:00:00
abstract：AIM:The aim of the present study was to investigate the impact of cyclin E expression on cancer-specific survival, as well as on conventional clinocopathological and prognostic factors in endometrial endometrioid adenocarcinoma. MATERIALS AND METHODS:The study consisted of 211 patients surgically treated for endometri...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-06-01 00:00:00
abstract：BACKGROUND:Pemetrexed and erlotinib represent different agents commonly used for the second-line treatment of patients with advanced-stage non-small cell lung cancer (NSCLC). PATIENTS AND METHODS:We analyzed data of 137 patients with advanced-stage non-squamous NSCLC treated with pemetrexed or erlotinib in the second ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-01-01 00:00:00
abstract：BACKGROUND:Resistance to multiple chemotherapeutic agents results from a variety of factors including a lack of apoptosis in tumor cells. To induce apoptosis in two types of tumor cells, RPMI-8226 and MG-63, a combination of chemotherapeutic agents and retroviral transduction of TRAIL was employed. MATERIALS AND METHO...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-07-01 00:00:00
abstract：BACKGROUND:Several types of human papilloma virus (HPV) have been associated with increased risk for epithelial malignancies. In light of a recently available vaccine that protects against persistent infection by certain HPV types (6, 11, 16, 18) and resulting neoplasias, the prevalence of HPV types was investigated in...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-07-01 00:00:00
abstract：BACKGROUND:Merkel cell carcinoma (MCC) is an aggressive KIT-positive cutaneous tumor. KIT mutations are considered to play a key role in the pathogenesis of various neoplasms, but have not been found so far in MCC. The aim of the present study was therefore to investigate the presence of KIT mutations in MCC. MATERIAL...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-03-01 00:00:00
abstract：BACKGROUND/AIM:ANRIL is a long noncoding RNA located on INK4 locus, which encodes p15 and p16 that cause G1 phase arrest in the cell cycle. ANRIL positively regulates proliferation of several kinds of cancer cells such as lung and gastric cancers. This study, examined the effect of ANRIL in head and neck squamous cell ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13564
更新日期：2019-08-01 00:00:00
abstract：BACKGROUND/AIM:The optimal treatment of liver metastases from gastric cancer (LMGC) remains uncertain. We retrospectively compared surgical treatment with chemotherapy alone and identified prognostic determinants. PATIENTS AND METHODS:We reviewed the records of 50 consecutive patients with LMGC: 25 patients with gastr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-02-01 00:00:00
abstract：:Four sorts of tumor proliferation-related markers were studied in 50 papillary carcinomas of the thyroid, and the results were correlated with the histologic subtypes of papillary thyroid carcinoma, which have been shown to be associated with the prognosis of the disease. The mitotic count analyzed by light microscopy...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Several studies have reported that high F18-fluorodeoxyglucose (FDG) uptake is predictive of poor prognosis and aggressive features in patients with breast cancer. While these studies evaluated the prognostic value for cases with high FDG uptake, they did not elucidate the meaning of FDG negativity in primary breast c...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-06-01 00:00:00
abstract：BACKGROUND:The antiemetic effects of serotonin receptor antagonists during chemoradiotherapy for solid tumors have never been reported. We have developed hyperthermo-chemo-radiotherapy (HCR) for esophageal cancer. However, with this treatment, the more potent the chemotherapy was, the more frequently emesis was experie...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：BACKGROUND:Nuclear morphometry can be expected to improve the distinction between benign and malignant lesions. PATIENTS AND METHODS:Forty fine-needle aspiration biopsy (FNAB) samples fixed in 50% ethanol were collected at the African Oncology Institute, Sabratha, Libya, during the period 2004-2007. All diagnoses repo...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-11-01 00:00:00
abstract：BACKGROUND:Small cell neuroendocrine cervical carcinoma (SCNCC) is a rare tumor that comprises 1-3% of cervical tumors. SCNCC exhibits clinical and biological characteristics of both cervical neoplasm (such as local aggressiveness and involvement of papillomavirus) and neuroendocrine small cell cancer of any site (such...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-02-01 00:00:00
abstract：:Basal level of asparagine synthetase mRNA in BALB3T3 cells was elevated when the cells were shifted from medium containing a high concentration (3.3 mM) of asparagine to one lacking asparagine. We then studied whether the expression of asparagine synthetase mRNA is also mediated through other asparagine-independent si...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：AIM:To analyze how patients experience Stereotactic guided vacuum-assisted breast biopsy (VABB) both physically and mentally. PATIENTS AND METHODS:Two hundred and eleven consecutive women underwent VABB using one of two different biopsy devices (ATEC® and Mammotome®). Patients were queried using a questionnaire. RESU...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-07-01 00:00:00
abstract：:During intercellular induction of apoptosis, transformed fibroblasts are eliminated through the action of neighbouring nontransformed cells. TGF-beta thereby plays three distinct and central roles. a) TGF-beta released by transformed cells or added exogenously to the assay system triggers nontransformed cells to relea...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1999-01-01 00:00:00
abstract：BACKGROUND/AIM:5-Aminolevulinic acid (5-ALA), a precursor of protoporphyrin IX (PpIX), is now used for photodynamic therapy (PDT) of pre-cancers of the skin and photodynamic diagnosis (PDD) of brain tumors. Sonodynamic therapy (SDT) of cancers with ultrasound has been studied using 5-ALA as a sonosensitizer. In this ar...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-07-01 00:00:00
abstract：BACKGROUND:The results of chemotherapy in patients with advanced, hormone-refractory prostate cancer (HRPC) have been disappointing. Mitoxantrone has been used in the past for palliation, but it does not prolong survival. It was recently demonstrated that docetaxel is able to improve median survival as compared to mito...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：:The effect of ionic strength on aclarubicin - DNA complexes was studied in comparison with its effect on daunorubicin - or doxorubicin - DNA complexes, using the spectrophotometric method. The hypochromic shift of aclarubicin, induced by its binding to native DNA, decreased to a lesser extent by the addition of Na+ th...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1988-05-01 00:00:00
abstract：:Ten patients with neuroblastoma were treated with a standard pulsed administration schedule of cis-platinum and etoposide (VP16-213). Two patients are experiencing good partial responses lasting over 8 months. One other patient had a partial response. Toxicity was mild and limited to high frequency hearing loss, tempo...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1986-11-01 00:00:00
abstract：BACKGROUND:Recently, it has become obvious that the HMGB1 protein can act as a proinflammatory and proangiogenic mediator when actively secreted by macrophages or passively released from necrotic cells playing an important role in the pathogenesis of several diseases including cancer. MATERIALS AND METHODS:The absolut...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-12-01 00:00:00
abstract：AIM:The aim of the present study was to evaluate the correlation between WT1 expression levels and clinical features, to investigate the prognostic value of WT1 expression and to use lentiviral constructs to examine whether overexpression of WT1 affects cell proliferation and invasion in ovarian cancer patients. MATER...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-05-01 00:00:00
