Abstract：
BACKGROUND:To identify immunosuppressive elements present in ovarian cancer associated ascites. PATIENTS AND METHODS:Ascites and plasma were obtained from ovarian, primary peritoneal or fallopian tube cancer patients. Surface markers were identified by fluorescence-activated cell sorting (FACS). Cytokine and chemokine concentrations were measured with LINCOplex microarrays. Antigen-specific T-lymphocytes from ascites and plasma were expanded with artificial antigen-presenting cells (aAPC). Cell-mediated immune response was assessed with chromium release assays. RESULTS:Samples were collected from 37 patients with advanced ovarian cancer. FACS was performed on 27 ascites specimens. A low CD4/CD8 ratio (<1.6) was seen in 13 patient samples and associated with significantly improved overall survival (p=0.040). LINCOplex evaluation of 22 paired ascites and plasma samples demonstrated significantly elevated levels of IL-6, IL-8, IL-10, IL-15, IP-10, MCP-1, MIP-1beta and VEGF and significantly reduced levels of IL-2, IL-5, IL-7, IL-17, PDGF-BB, and RANTES in ascites compared to plasma (p<0.05). Autologous ovarian cancer cell lysis with T-lymphocytes from ascites was limited. Although aAPC stimulation resulted in effective expansion of antigen specific T-cells from peripheral lymphocytes (35-fold), only limited expansion was noted from ascites-derived lymphocytes (10-fold). CONCLUSION:Ovarian cancer-associated ascites may provide an immunosuppressive environment. A high CD4/CD8 ratio, which may indicate the presence of regulatory T-cells, is associated with poor outcome. Reduced IL-2 and elevated IL-6 and IL-10 levels favor a Th2 inhibitory immune response. This immunosuppressive climate may explain our observation of non responsiveness in ascites derived T-cells.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Giuntoli RL 2nd,Webb TJ,Zoso A,Rogers O,Diaz-Montes TP,Bristow RE,Oelke Msubject
Has Abstractpub_date
2009-08-01 00:00:00pages
2875-84issue
8eissn
0250-7005issn
1791-7530pii
29/8/2875journal_volume
29pub_type
杂志文章abstract：:The purpose of this analysis was to study the association between the quantity of free circulating DNA and clinical variables in 99 advanced non-small cell lung cancer patients (NSCLC). The quantification in the serum of the gene of the catalytic fraction of telomerase (hTERT) by RT-PCR was used as a reference of the ...
journal_title：Anticancer research
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journal_title：Anticancer research
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journal_title：Anticancer research
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journal_title：Anticancer research
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doi：
更新日期：2014-11-01 00:00:00
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更新日期：2011-04-01 00:00:00
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doi：
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更新日期：2007-03-01 00:00:00
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pub_type： 杂志文章
doi：
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doi：
更新日期：2013-09-01 00:00:00
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更新日期：2009-02-01 00:00:00
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更新日期：2015-12-01 00:00:00
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doi：
更新日期：1994-11-01 00:00:00
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更新日期：2016-08-01 00:00:00
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更新日期：2013-11-01 00:00:00
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pub_type： 临床试验,杂志文章
doi：
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