Abstract:
:Megakaryocyte polyploidization responds to platelet demand and results from the lack of cytoplasmic separation while the nucleus keeps dividing. In normal telophase, the plane of the actin constriction ring is determined by the tubulin spindle. In order to investigate the role of tubulin in the megakaryocyte polyploidization, two cell lines with megakaryocyte properties (DAMI and HEL) were incubated for 4 days in the presence or absence of colchicine (10 ng/ml), an inhibitor of the tubulin spindle. As compared to control conditions, cell cultured in the presence of colchicine reveal an augmentation of cell size, the apparition of multilobed nuclei and an increase in the cytoplasm basophilia, suggesting a megakaryocyte morphology. Furthermore, when cells are cultured in the presence of colchicine, diameters measured by morphometry augment from 17.4 microns +/- 1.7 to 34.5 microns +/- 2.0 and from 27.3 microns +/- 0.3 to 40.2 microns +/- 0.6 for DAMI and HEL cell lines, respectively (p < 0.05 by t-test). After four days of culture in the presence of colchicine, cells undergo arrest proliferation. Ploidy measured by flow cytometry, shows that control cells predominantly diploid (2N) become polyploid with the appearance of 8N, 16N and 32N cells after addition of colchicine. Moreover, the endomitotic index ¿mean of (log2 DNA content expressed in N)-l¿ increases significantly from 0.5 +/- 0.1 to 1.2 +/- 0.1 and from 0.6 +/- 0.1 to 1.4 +/- 0.0 after treatment with colchicine for the DAMI and HEL cell lines, respectively. To identify the nature of the molecules involved in this phenomenon, both forms of actin (monomeric, G- and polymerized, F-) were evaluated by a DNase I inhibition assay. G-actin contents in pg per 10(6) cells are 13.0 pg +/- 2.8 (m +/- SEM) and 1.0 pg +/- 0.1 for unstimulated DAMI and HEL cells. F-actin contents per 10(6) cells are 5.8 pg +/- 1.5 and 0.1 pg +/- 0.0 for DAMI and HEL cells. The addition of colchicine for four days of culture significantly increased the G-actin content (251% and 475% of controls) and F-actin content (170% and 619% of controls) for DAMI and HEL cell lines, respectively. In contrast, the G/F-actin ratio was not affected by colchicine. DAMI cells from each ploidy class were then sorted on an ELITE Coulter and assayed for actin content. While total actin, G-actin and F-actin per cell were augmented in polyploid cells cultured with colchicine, there was a reduction in G-, F- and total actin contents per diploid equivalent when cells become polyploid. In conclusion, these data suggest that inhibition of the tubulin spindle by colchicine induces polyploidization of megakaryocytes by a reduction of both forms of actin, possibly by preventing the actin constriction ring in the telophase.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Baatout S,Chatelain B,Staquet P,Symann M,Chatelain Csubject
Has Abstractpub_date
1998-05-01 00:00:00pages
1553-61issue
3Aeissn
0250-7005issn
1791-7530journal_volume
18pub_type
杂志文章abstract::UGP (Urinary gonadotropin peptide) also know as urinary gonadotropin fragment (UGF) or the beta-core of hCG (c beta hCG), was identified as a peptide with a molecular weight of 10.5 kD, having the same amino acid sequence as the core section of the beta-subunit of human chorionic gonadotropin. UGP has been found in no...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::D-Penicillamine, a structural analog of cysteine, has the ability to chelate metal ions and reacts with cysteine. We have shown earlier that D-Penicillamin is a potential inhibitor of tat-mediated transactivation of HIV-1-LTR (14) and possesses anti-HIV-1 activity (23). Following this approach, we evaluated the anti-t...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::A pharmacologically relevant modelling was attempted for screening of new molecules potentially active against non-small-cell lung cancers (NSCLCs). It was in this way that we investigated the in vivo chemosensitivity firstly of three human NSCLCs grafted onto nude mice, secondly of their in vitro primocultures, and t...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract:BACKGROUND/AIM:Only 30-50% of patients with sentinel lymph node (SLN) metastases present with further axillary lymph node metastases. Therefore, up to 70% of patients with positive SLN are overtreated by axillary dissection (AD) and may suffer from complications such as sensory disturbances or lymphedema. According to ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12694
更新日期:2018-07-01 00:00:00
abstract::Sentinel lymph node biopsy (SLNB) is currently the suggested axillary staging procedure in patients with early-stage breast cancer (BC) and usually requires intraoperative frozen-section (FS) examination of the removed nodes. However, other techniques, such as touch imprint cytology (IC), real-time reverse transcripta...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-08-01 00:00:00
abstract::The effects of an anti-CD3 mAb on induction of non-MHC restricted cytolysis was investigated. Peripheral blood mononuclear cells (PBMC) from normal donors (29) and cancer patients (18) were cultured in 100 U/ml of interleukin-2 (rIL-2) with and without anti-CD3 mAb (OKT3, 10 ng/ml) for the first 48 hours of incubation...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::In this study, we investigated the cytotoxicities of flavone (F01), 3-hydroxyflavone (F02), 6- hydroxyflavone (F03), 7-hydroxyflavone (F04), 3,6-dihydroxyflavone (F05), 5,7-dihydroxyflavone (F06) and 5,6,7-trihydroxyflavone (F07) to human cancer cells including P- glycoprotein (Pgp)-expressing HCT15 cells and its mult...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::Chloroethyl-nitrosourea is a potent chemotherapeutic agent for brain tumors. However, acquired resistance to this drug has become a serious problem for the treatment of patients. Previously, we established an animal model resistant to nitrosourea (Anticancer Res 19: 5313-5318, 1999). In this study, we evaluate the eff...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract::Tenascin is a multifunctional extracellular matrix glycoprotein generally expressed in epithelial tumors at sites of epithelial tumor-mesenchymal tissue interface. Studies so far have indicated that mesenchymal cells are a major source of tenascin in tumor tissues. The present study evaluated biopsies and surgically r...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract:AIM:To investigate the discordance in the programmed cell death-ligand 1 (PD-L1) expression between primary and metastatic tumors and analyze the association between the discordance and the clinical factors in non-small cell lung cancer (NSCLC) patients. PATIENTS AND METHODS:Twenty-one NSCLC patients who underwent sur...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11813
更新日期:2017-08-01 00:00:00
abstract::The LAI reactivity of a colon organ specific neoantigen (OSN) was recovered from the urine of advanced colon cancer patients. In this study three physico-chemical steps were employed; precipitation by 80% ammonium sulfate, ion exchange, and molecular sieve chromatography. Each isolate was tested for activity and speci...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1986-07-01 00:00:00
abstract::Vesnarinone (3,4-dihydro-6-[4-(3,4-dimethoxybenzoyl)-1-piperazinyl]-2(1H)- quinolinone), a quinolinone derivative, is an orally active inotropic agent used in Japan for the treatment of chronic heart failure. Recently, it has been reported that vesnarinone induces differentiation and apoptosis in certain types of leuk...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Malignant gliomas are often treated with cisplatin (cis-diamminedichloroplatinum(II), CDDP) and radiation but results remain unsatisfactory. To investigate whether CDDP induces radioresistance in glioma, T98G human glioblastoma cells were pretreated 5 times with 10(-6)M CDDP for 24 hours and then the sensitivity of wi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
abstract::Chk1 (checkpoint kinase 1) is a serine-threonine kinase that is critical for G2/M arrest in response to DNA damage. Chk1 phosphorylates Cdc25C at serine-216, a major regulatory site, in response to DNA damage. Furthermore, Chk1 also phosphorylates Cdc25A on serine 123 which accelerates its degradation through the ubiq...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2004-11-01 00:00:00
abstract::Flow cytometric DNA analysis was performed in 67 tubular, tubulovilous and villous rectal adenomas with different degree of dysplasia. One of the adenomas contained a focus of early invasive carcinoma. All but one of the 67 adenomas had near diploid DNA histograms; the exception was a tubular adenoma with severe dyspl...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
abstract::We have previously reported that lignin carbohydrate complex and E. coli lipopolysaccharide (LPS), but not their precursors or (-)-epigallocatechin-3-gallate (EGCG), enhanced the nitric oxide (NO), citrulline and asparagines production by the mouse-macrophage-like cell line Raw264.7. Here the EGCG inhibition of NO pro...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2008-05-01 00:00:00
abstract:AIM:The purpose of this study was to identify patient-, facility-, disease-, and treatment-specific characteristics that increase mortality in patients with upper limb osteosarcoma. PATIENTS AND METHODS:The National Cancer Data Base (NCDB) was queried for bone cancer. With Cox regression, the demographic, facility, tu...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13693
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND/AIM:4H-1-Benzopyran-4-ones (chromones) have provided backbone structure for the chemical synthesis of potent anticancer drugs. In this study, the cytotoxicity of fifteen 2-(N-cyclicamino)chromone derivatives was investigated and subjected to quantitative structure-activity relationship (QSAR) analysis. MATE...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12674
更新日期:2018-07-01 00:00:00
abstract::Five functionalized benz(c)acridines - 5,6-dimethylbenz(c)acridine; 5,6,7-trimethylbenz(c)acridine; 7-chloro-5,6-dimethylbenz(c)acridine; 7-amino-5,6-dimethylbenz(c)acridine; 7-oxo-5,6-dimethylbenz(c)-acridine and 5,6-dimethylbenz(a)phenazine - were tested for mutagenic activity in the Ames Salmonella typhimurium assa...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract::Whether serum testosterone (T) can become an adjunct test able to validate the PSA-weighted risk of prostate cancer (PR.CA) in the "grey" diagnostic area (PSA =3.0 to <10.0 ng/ml) was investigated. Seven hundred and eighteen men participated in a prostate screening program using the cutoff PSA value of > or =3.0 ng/ml...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract::Cancer patients suffer from cancer-induced bone pain, hypercalcemia, and reduced quality of life caused by pathological fractures. Many of these complications related to cancer can be treated, or at least controlled, using new anticancer agents. Recently, two agents used initially to treat osteoporosis demonstrated di...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2013-08-01 00:00:00
abstract::The oxazaphosphorines cyclophosphamide (CP) and ifosfamide (IF) are alkylating agents that require bioactivation via cytochrome (CYP) P450 isoenzymes including CYP2C9 enzymes. The present study investigated CYP2C9 in regard to its allelic variants in 23 tumor samples (10 breast tumors, 1 breast tumor cell line, 5 brai...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Overexpression of p53-protein appears to be a common event in primary breast cancer. It has been proposed that the presence of elevated levels of this protein may be an independent prognostic factor and may be important for the ability of a tumor to metastasize. This study was performed to evaluate the influence of im...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::The metabolic transformations of labeled pyridoxine by hepatoma cells were studied. Buffalo rats were fed ad libitum a commercially prepared pyridoxine sufficient diet for 20 days at which time they were inoculated intramuscularly with hepatoma 7777 cells in both hind leg muscles. After tumor development, 50 microCi [...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::Calcitriol is a potent antiproliferative agent against various tumour cells in vitro. Here, the results of a study on vitamin D compounds (calcitriol's analogues PRI-1906 and PRI-2191) as potential agents in combined antitumour therapy in vitro are presented. Applying antiproliferative SRB and MTT assays, the growth i...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract:BACKGROUND:The process of tumour invasion and subsequent metastasis represents the most lethal aspect of cancer and is responsible for the majority of deaths among cancer patients. Our objective was to identify invasion-specific genes in a series of isogenic breast cancer cell lines of differing invasive potential usin...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2007-05-01 00:00:00
abstract::The objective of this study was to discover whether the peptides LRMK and LRMK-Ava linked to the N-terminus of peptides HER-2 (774-788) and HER-2 (776-788), respectively, help differentiation of E75-TCR(+)CD8(+) cells. Activation was quantified in terms of proliferation of E75-TCR(+)CD8(+) cells expressing high, mediu...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-07-01 00:00:00
abstract::The clinical features of 10 cases with the adjunct of a literature review of primary neuroendocrine carcinoma of the skin (Merkel cell tumor) are reported. This cancer arises in the dermis and subcutaneous tissue of elderly individuals. Natural history is characterized by local recurrences (40%), regional lymph-nodes ...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Melanoma cells express the nuclear vitamin D receptor (VDR), indicating that malignant melanoma represents a promising target for treatment with 1,25-dihydroxyvitamin D3 (1,25(OH)(2)D(3)) or its analogs. We previously showed that some melanoma cell lines are resistant to the antiproliferative effects of 1,25...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
abstract::The dimers bis-EUG, bis-MMP, bis-BHA, bis-BMP and bis-DBP were synthesized from the monomers 4-allyl-2-methoxyphenol (EUG), 2-methoxy-4-methylphenol (MMP), 2-t-butyl-4-methoxyphenol (BHA), 2-t-butyl-4-methylphenol (BMP) and 2,4-di-t-butylphenol (DBP), respectively. The stoichiometric factors (n; number of free radical...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2004-09-01 00:00:00