Abstract：
BACKGROUND:A critical step in the delivery of nanomedicines to tumour cells is transporting these particles through the extracellular matrix. Tumour-specific anticancer agents, such as encapsulated drugs, proteins, and genes, show low uptake in tumour tissue. It is not clear whether the collagen network or the glycosaminoglycan gel plays the most important role in limiting the interstitial transport of macromolecules. Therefore, we measured the effect of the collagen- and hyaluronan-degrading enzymes, collagenase and hyaluronidase, on interstitial diffusion. MATERIALS AND METHODS:Human osteosarcomas were grown as multicellular spheroids and xenografts in dorsal skinfold window chambers. Diffusion of fluorescein isothiocyanate (FITC)-dextran molecules was measured by fluorescence recovery after photobleaching based on two-photon scanning laser excitation. RESULTS:Collagenase, hyaluronidase, and relaxin increased the diffusion coefficient of the 2-MDa FITC-dextrans in the spheroids, but 150-kDa FITC-dextran diffusion was not affected by the enzymatic treatment. In tumour tissue in vivo, collagenase and hyaluronidase increased the diffusion of the 150-kDa FITC-dextrans. In xenografts, anomalous diffusion occurred, whereas only free diffusion was seen in spheroids. CONCLUSION:The results indicate that the collagen network has a greater impact on the interstitial diffusion of macromolecules in tumour tissue than the hyaluronan gel.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Eikenes L,Tufto I,Schnell EA,Bjørkøy A,De Lange Davies Csubject
Has Abstractpub_date
2010-02-01 00:00:00pages
359-68issue
2eissn
0250-7005issn
1791-7530pii
30/2/359journal_volume
30pub_type
杂志文章abstract：BACKGROUND/AIM:Intrapelvic bleeding after extended pelvic surgery is fatal, but few reports have addressed this serious situation. The objective of the present study was to evaluate the short-term clinical outcome of this complication. PATIENTS AND METHODS:This study was a retrospective medical chart review of 130 pat...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2014-06-01 00:00:00
abstract：:We investigated the effects of an exogenous cdk inhibitor, butyrolactone-I, on cell growth inhibition, apoptosis induction, and the regulation of apoptosis in pancreatic cancer cells with mutated p53. Cell growth was dose-dependently inhibited by Butyrolactone-I in PANC-1 and AsPC-1 cells. Phosphorylation of pRb and C...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11276
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-09-01 00:00:00
abstract：:The magnetic resonance (MR) findings in malignant melanoma of soft tissues, also called clear cell sarcoma of tendons and aponeuroses, have been described as a focal abnormality with a specific MR pattern of increased signal intensity (relative to normal muscle) on T1 weighted sequences and variably decreased signal i...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2000-09-01 00:00:00
abstract：BACKGROUND:C-reactive protein (CRP) is the prototypical biomarker of inflammation. Genetic variations within the CRP gene have been shown to be associated with alterations of CRP expression and prognosis in cancer patients. The purpose of this study was to evaluate the association between four polymorphisms of the CRP ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-06-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:Variants of the Bomirski family of hamster melanomas whose proliferative rates differ inversely with the genetically determined degree of melanogenesis were probed for two proteins critical in melanogenesis: tyrosinase and catalase-B (gp 75). The parental black tumor Ma contained both proteins in abundance. The amelan...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
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doi：
更新日期：2014-10-01 00:00:00
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更新日期：2019-09-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：BACKGROUND:Human papillomavirus (HPV) is a risk factor for tonsillar squamous cell carcinoma (TSCC) and the presence of HPV is correlated to a better clinical outcome. To find additional biomarkers that, together with HPV, predict clinical outcome, the aim of the present study was to evaluate epidermal growth factor re...
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doi：
更新日期：2013-04-01 00:00:00
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doi：
更新日期：2006-01-01 00:00:00
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pub_type： 杂志文章
doi：
更新日期：2010-06-01 00:00:00
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pub_type： 杂志文章
doi：
更新日期：2015-05-01 00:00:00
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pub_type： 杂志文章
doi：10.21873/anticanres.13198
更新日期：2019-02-01 00:00:00
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doi：
更新日期：2015-04-01 00:00:00
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doi：
更新日期：2005-03-01 00:00:00
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doi：
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doi：
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journal_title：Anticancer research
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doi：
更新日期：1996-09-01 00:00:00
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doi：
更新日期：2004-07-01 00:00:00
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doi：
更新日期：2009-10-01 00:00:00
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更新日期：1998-11-01 00:00:00
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更新日期：2013-02-01 00:00:00
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doi：
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