Abstract:
BACKGROUND:It is hypothesised that hypermethylation of the CpG island in the promoter region in several tumour suppressor genes contributes one of the mechanisms for silencing or down-regulating the expression of the tumour suppressor genes. One of the putative tumour suppressor genes, the E-cadherin gene, has shown down-regulation in nasopharyngeal carcinoma (NPC). The aim of this study was to explore the methylation profiles of the E-cadherin promoter region in NPC patients. MATERIALS AND METHODS:Fifteen NPC paraffin-embedded tissue sections were included in the study accompanied by two cell lines, MCF7 and MDA435, as controls. The technique of bisulfite genomic sequencing was used to determine the methylation patterns of the 29 CpG dinucleotides along the E-cadherin promoter region. DNAs were extracted from the samples, and then were subject to bisulfite conversion. Nested polymerase chain reactions were performed on the modified DNAs, the sequences of which were determined by direct sequencing. RESULTS:A pilot study ascertained the correlation between reduced E-cadherin expression and hypermethylation of the E-cadherin promoter region from the E-cadherin (+) MCF7 and the E-cadherin (-) MDA435 cell lines. The experimental study revealed that, among 10 NPC cases in which DNA could be successfully extracted, 6 (60%) displayed methylation patterns at two or more CpG sites (range: 2-11, 6.8-37.9%). Five of them (83.3%) were comprised of at least 2 transcription enhancing factor sites. Two of the 4 cases (50%) with methylated 4th CpG site (HpaII site) and 3 of the 4 cases (75%) with methylated 11th CpG site (GC region) had grades 3 or 4 level of methylation. CONCLUSION:The data indicated that hypermethylation of the E-cadherin promoter region occurred in a large proportion of NPC patients, and frequent methylation of specific CpG sites associated with transcription enhancing elements suggested that this phenomenon may contribute part of the mechanisms for silencing or down-regulating E-cadherin gene expression in NPC.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Kao RH,Huang LC,Hsu YHsubject
Has Abstractpub_date
2002-11-01 00:00:00pages
4109-13issue
6Ceissn
0250-7005issn
1791-7530journal_volume
22pub_type
杂志文章abstract::This randomized study was designed to compare the efficacy and toxicity of MMV chemotherapy (mitomycin C(MMC), methotrexate (MTX) and vincristine(VCR)), MMVM (MMV+medroxyprogesterone acetate(MPA)) and MMVP (MMV+prednisolone(P)), and to evaluate potential additional effects of MPA or P to the chemotherapy in doxorubici...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1995-09-01 00:00:00
abstract::Smokeless tobacco consumption is causally associated with oral cavity cancers; however, extensive cytogenetic studies have not been done. In the present study, individuals consuming dry snuff or tobacco with lime have been studied for frequency of micronucleated cells (MNC) in exfoliated buccal mucosa and chromosome a...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
abstract:BACKGROUND/AIM:A prospective study was designed to investigate the effects of anesthesia, particularly that of the one-lung ventilation procedure (OLV), on the expression of hypoxia-inducible factor 1alpha (HIF1alpha) in patients with lung carcinomas and pneumothorax. MATERIALS AND METHODS:The immunohistochemical expr...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-04-01 00:00:00
abstract:BACKGROUND:DNA repair is a crucial phenomenon that maintains the chromosome integrity of genome which are continuously damaged by endogenous and exogenous alkylating agents. If the damaged DNA is not repaired, it may lead to mutation, chromosomal aberration, aging and cancer. N-methylpurine-DNA glycosylase (MPG), a ubi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2000-09-01 00:00:00
abstract::Exposure of human promyelocytic leukemic HL-60 cells to millimolar concentration of sodium ascorbate induced apoptotic cell death. The extent of apoptosis induction was a positive function of temperature at the time of exposure. The incorporation of [1-14C] ascorbic acid into the cytosolic fraction of HL-60 cells was ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
abstract:BACKGROUND/AIM:Despite an expected prognostic disadvantage for upper tract versus lower tract metastatic urothelial carcinomas (UTUC/LTUC), only few studies have been conducted to elucidate potential differences in chemotherapy treatment. PATIENTS AND METHODS:A post-hoc subgroup analysis of a non-interventional study ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12098
更新日期:2017-11-01 00:00:00
abstract:BACKGROUND:This study aimed to elucidate the prognostic value of vimentin expression in patients with intrahepatic cholangiocarcinoma (ICC). PATIENTS AND METHODS:A retrospective analysis of 21 patients who underwent resection for ICC was conducted. Vimentin expression was positive when single tumour cells or cell clus...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-06-01 00:00:00
abstract::This report describes a precise molecular analysis of a rare case of Philadelphia chromosome (Ph) positive acute myeloid leukemia (AML) (FAB classification M2). Phenotypic markers were positive for cells of the myeloid lineage, but negative for B cell and T cell lineage. The leukemic cells carried a Philadelphia chrom...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND/AIM:The aim of the present study was to determine the efficacy of trabectedin combined with FOLFIRI (irinotecan, leucovorin and 5-fluorouracil) on a colorectal cancer (CRC) patient-derived orthotopic xenograft (iPDOX) mouse model. MATERIALS AND METHODS:A CRC tumor from a patient previously established in nu...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13860
更新日期:2019-12-01 00:00:00
abstract::The relationship between production of Colony-Stimulating Factor (CSF) and metastasis has been investigated in the TS/A murine model. CSF production was determined in TS/A cell variants isolated through serial in vivo selection of lung metastatic nodules induced by intravenous or subcutaneous injection of tumor cells ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1987-07-01 00:00:00
abstract:BACKGROUND:Tyrosine kinase inhibitors (TKIs) directed against epidermal growth factor receptor (EGFR) are important in the treatment of non-small cell lung cancer (NSCLC), especially those harboring EGFR mutations. But little is known regarding the clinical value of serum tumor marker levels measured prior to treatment...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12018
更新日期:2017-10-01 00:00:00
abstract::Independently of tumour and treatment modulation, the host immune response status plays an important role in the clinical outcome of patients with cancer. The influence of single nucleotide polymorphisms (SNPs) and adjuvant radiotherapy (RT) on the systemic immune response status of patients with breast cancer was inv...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13240
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVE:The authors report their experience in patients with adjuvant systemic 2-interferon with the aim of defining the effectiveness, side-effects, indications and limitations of this treatment. MATERIALS AND METHODS:From January 1989 to December 1996, 123 patients with genital, anorectal and perineal HPV lesions ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2002-03-01 00:00:00
abstract:BACKGROUND/AIM:Nodal staging is used in colorectal cancer (CRC) to determine which patients should receive adjuvant chemotherapy. The aim of this study was to evaluate the role of indocyanine green fluorescence imaging (ICG-FI) in sentinel lymph node (SLN) detection compared to the standard technique. MATERIALS AND ME...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11048
更新日期:2016-09-01 00:00:00
abstract:BACKGROUND/AIM:Neuroendocrine neoplasms (NEN) of the female genital tract account for 2% of gynecological cancers. The aim of this study was to share our experience of 11 primary neuroendocrine neoplasms of the ovary. PATIENTS AND METHODS:All patients who presented and/or were treated at our Institution with histologi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2016-03-01 00:00:00
abstract:AIM:The aim of the study was to identify novel biomarkers that are vital for improving management of patients with gastric cancer (GC). MATERIALS AND METHODS:An RNA-sequencing analysis was conducted using gastric tissue from patients with metastatic GC. In vitro cell functions were evaluated by siRNA-mediated knockdow...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13820
更新日期:2019-11-01 00:00:00
abstract:BACKGROUND:Previous reports indicate that the p53 yeast functional assay is a highly sensitive method of detection of p53 mutations in clinical specimens. Our earlier report (1) showed a 35.4% p53 mutation frequency in Hong Kong colorectal carcinoma (CRC) patients, when conventional molecular screening techniques were ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:Randomized controlled trials had demonstrated local therapy, such as radiotherapy, can improve outcomes of patients with lung cancer with oligometastatic disease (OMD). However, the definition of OMD is not uniform and the European Society for Radiotherapy and Oncology (ESTRO) and European Organisation for R...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.14609
更新日期:2020-10-01 00:00:00
abstract::The purpose of this study was to elucidate the behavior of signal transduction activity in rat and human carcinoma cells. Signal transduction activity was measured by the steady-state activity of the three enzymes involved in the conversion of 1-phosphatidylinositol (PI) to IP3, PI 4-kinase, PI 4-phosphate 5-kinase, a...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract::The polysaccharide produced by the red microalga Porphyridium sp. was highly inhibitory for cell transformation of mouse fibroblast cells by an MSV-124 virus stock. This inhibition was most effective if the polysaccharide was added 2 h before or at the time of infection. The finding that the inhibition of cell transfo...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-05-01 00:00:00
abstract:BACKGROUND:Using three commercially available, unmodified PSA assays, 917 sera of 355 patients after radical prostatectomy underwent native and lyophilisation-concentrated PSA-detection to evaluate the benefit of serum concentration as a tool to increase sensitivity for earlier detection of recurrent prostate cancer af...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::The metastatic involvement of the spleen in epithelial ovarian cancer is rare and usually reflects late disseminated disease. Isolated parenchymal metastasis in the spleen is an extremely unusual event, in fact in most cases in medical literature, the spleen was involved as part of diffuse peritoneal carcinomatosis. W...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2002-11-01 00:00:00
abstract:BACKGROUND:The knowledge of direct effects of β-glucans on tumor cells is limited. This study evaluated the impact of a soluble yeast-derived beta-(1-3),(1-6)-d-glucan, containing a fraction of aggregated sugar polymers, on viability, proliferation and expression of CD86 of the human B-cell lymphoma cell lines Daudi an...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-12-01 00:00:00
abstract:BACKGROUND/AIM:Ouabain, a plant-derived product/substance with Na+/K+-ATPase inhibiting properties, has been shown to exert anti-cancer activity on human cancer cells. This is the first study to investigate the effect of ouabain on apoptotic cell death of human osteosarcoma-derived U-2 OS cells. MATERIALS AND METHODS:...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12205
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Natural killer (NK) cells are a third population of lymphocytes that can kill certain tumor cells. This killing is regulated by signals received through activating and inhibitory receptors. 2B4 (CD244), a member of the CD2 subset of Immunoglobulin superfamily, was identified as an activating receptor on NK c...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract::Metastatic pancreatic malignant melanoma is considered to be a highly aggressive neoplasm, and only few metastasectomies for lesions originating from the skin or the ocular region have been reported. We report a case of resection of pancreatic metastasis of malignant melanoma originating from the nasal cavity. An isol...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2013-02-01 00:00:00
abstract::The accuracy of Tc-99m MIBI scintimammography depends on the uptake in cancer cells being greater than surrounding normal tissue. The aim of this study was to determine which were the dominant factors affecting uptake of Tc-99m MIBI in breast cancer cells in vivo. The tumour to background ratio (TBR) was measured in 7...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1997-05-01 00:00:00
abstract::The p53 gene product suppresses both tumor cell growth and the cellular transformation process promoted by oncogenes. Although several genes are known to be positively regulated by p53, it is unclear how many gene expression events are involved in p53-mediated growth arrest and apoptosis. Two-dimensional gel electroph...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
abstract::P-glycoprotein has been identified in mast cells stabilized in culture as well as in rat peritoneal mast cells, and is primarily concentrated on the granular membrane. This study aimed to define the role of this protein in the transport and accumulation of doxorubicin in mast cell granules and in its histamine releasi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract:BACKGROUND:ProPSA has been suggested for the detection of preferentially aggressive prostate cancer (PCa). We report on the use of proPSA and free PSA to enhance preoperative staging and grading. PATIENTS AND METHODS:Serum samples from 376 PCa patients within the PSA range 1-25 microg/l who underwent radical prostatec...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:2007-07-01 00:00:00