Abstract:
:In order to improve the knowledge of prolactin receptors (PRL-R) in human breast tumors, we studied PRL-R modulation by lactogenic and steroid hormones in the PRL-R rich human breast cancer cell-line, T-47 D. The PRL-R were assayed on a preparation of cell total membranes. We demonstrated an abnormal homologous in vitro regulation of PRL-R. Concentrations of human growth hormone (hGH) greater than 500 ng/ml were required to cause a decrease in PRL-R, with a maximal down-regulation at 2000 ng/ml and for 48 hours. Human placental lactogen (hPL) induced a decrease in PRL-R at concentrations greater than 500 ng/ml but later than hGH; ovine prolactin (oPRL) had no effect on PRL-R. Moreover, we also demonstrated that progestins specifically modulated the expression of PRL-R in T-47D cells: Org 2058, a synthetic progestin induced a statistically significant increase in PRL-R after a twenty-four hour incubation period: this effect was already observed at 10(-9) M and was maximal for 10(-6) and 10(-5) M (186% +/- 3.5% (+/- SEM) for total PRL-R). At 10(-6) M, the stimulation occurred early at three hours and was maximal at twenty-four hours. Conversely estradiol (10(-9) to 10(-6) M), cortisol (10(-9) to 10(-6) M), dexamethasone (10(-9) to 10(-5) M) and RU 486 (10(-9) to 10(-5) M), a progestin and glucocorticoid antagonist, had no effect on PRL-R levels. The Org 2058 PRL-R stimulation was abolished in the presence of RU 486. The abnormal PRL-R down-regulation in the human breast cancer cell-line, T-47D, may contribute a growth advantage to these malignant cells over normal tissues. The progestin PRL-R dependence suggests that high levels of PRL-R may reflect a functional progesterone receptor (Pg-R) and a highly hormone-dependent-phenotype of the tumor. These results support a potential role of PRL in the etiology of breast tumors and may have important implications in the management of human breast cancer.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Leroy-Martin B,Peyrat JPsubject
Has Abstractpub_date
1989-05-01 00:00:00pages
631-6issue
3eissn
0250-7005issn
1791-7530journal_volume
9pub_type
杂志文章abstract:BACKGROUND:A recent study suggested that the p53Arg (at residue 72) homozygous genotype could be a potential genetic risk factor for cervical cancer among white women. To confirm this result we examined the proportion of p53 genotypes in a larger number of patients with cervical cancer and in patients with squamous int...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::We examined 11 patients (9 male and 2 female, age 41 +/- 13) presenting astrocytomas of different malignancy grade (WHO) with single voxel (PRESS) and double voxel (STABLE) spectroscopy techniques. The content of choline in the tumors was determined as a function of their grade of malignity and a significant correlati...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
abstract::Despite progress in chemotherapeutic agents, non-small cell lung cancers (NSCLC) still have a poor survival rate. Thus, development of new therapeutic strategies, specifically against cancer cells is still required. For this purpose, we treated the non-small cell lung cancer cell line NSCLC-N6 with the natural product...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-09-01 00:00:00
abstract::Due to the high recurrence rates of ovarian carcinoma, the treatment of recurrent disease is currently one of the most challenging topics in the clinical setting. Ovarian cancer patients who do not respond to initial chemotherapy or who relapse after achieving a response are generally incurable. Treatment goals after ...
journal_title:Anticancer research
pub_type: 杂志文章,多中心研究
doi:
更新日期:2009-07-01 00:00:00
abstract:AIM:To compare clinical outcome in patients with Child-Pugh C hepatocellular carcinoma (HCC) treated with non-transplant therapies and those treated with best supportive care. PATIENTS AND METHODS:A total of 182 patients with HCC with Child-Pugh C cirrhosis were analyzed. Patients were classified into two groups: pati...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-06-01 00:00:00
abstract::The purpose of this study was to determine whether the degree of anchorage-independent growth of rodent or human cells in increasing concentrations of agarose correlated with successful transfection of the cells with an activated c-Ha-ras oncogene and tumorigenicity in nude mice. NIH 3T3 cells, C3H 10T1/2 fibroblasts,...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:Altered retinoblastoma gene expression has been found in various types of cancers including non-small cell lung cancer (NSCLC) and some reports have shown it also to be associated with a poor clinical prognosis in NSCLC. MATERIALS AND METHODS:We evaluated the expression of Rb protein by immunohistochemistry...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2001-05-01 00:00:00
abstract::Metallothioneins (MT) are low molecular weight cysteine-rich proteins, present in a wide variety of eukaryotes. Although their physiological function is not entirely understood, recently it was found that in vitro human MTs (hMTs) expression prevents apoptosis. In the present study, the apoptosis preventing effect of ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract:BACKGROUND:Docetaxel is a very active drug against breast cancer, but at the standard dose causes severe myelosuppression. In order to reduce the toxicity while maintaining the activity, weekly docetaxel administration was tested. PATIENTS AND METHODS:We enrolled 30 patients with metastatic breast cancer, who had been...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:2003-03-01 00:00:00
abstract:BACKGROUND:DNA repair capacity may be an important factor in determining both individual susceptibility to cancer and the response to cancer therapy. The aim of this work was to compare DNA damage and the repair process in cells originating from healthy donors and cancer patients. MATERIALS AND METHODS:Using the micro...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract:BACKGROUND/AIM:In patients with soft tissue sarcoma (STS), distinguishing small metastatic pulmonary nodules from benign ones remains difficult. This study aimed to determine the accuracy of 18F-fluorodeoxyglucose positron emission tomography/computed tomography (FDG-PET/CT) in detecting pulmonary metastasis and identi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12638
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND:Chemotherapy for collecting duct carcinoma (CDC) has demonstrated only limited efficacy in the advanced setting. The present study evaluated the activity of targeted therapies in metastatic CDC. PATIENTS AND METHODS:We evaluated a cohort of 384 consecutive patients with metastatic renal cell carcinoma (mRCC...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-02-01 00:00:00
abstract:BACKGROUND:Calpains (CAPN) are intracellular, non-lysosomal cytoplasmic cysteine endopeptidases and they are expressed ubiquitously. Their endogenous specific inhibitor is calpastatin. When calcium is present, calpastatin and calpain attach to each other, inhibiting the protease. The calpain system plays an important r...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-07-01 00:00:00
abstract:BACKGROUND:The effects of resveratrol (a natural polyphenol) on angiogenesis in rat gliomas were investigated by color Doppler ultrasound (CDUS). The correlation among the tumor growth rate, macroscopic angiogenesis measured by CDUS and microscopic angiogenesis was assessed by immunohistochemical staining. MATERIALS A...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-03-01 00:00:00
abstract:BACKGROUND:We herein report a comparison of the radiolabels 177Lu and 125I bound to the monoclonal antibody HH1 that targets the CD37 antigen expressed on non-Hodgkin B-cell lymphomas. MATERIALS AND METHODS:Mixtures of 177Lu and 125I-labeled HH1 antibody were co-injected into nude mice carrying Ramos xenografts and th...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-07-01 00:00:00
abstract::Urachal cancer is a rare malignancy and the standard treatment is surgical resection. The prognosis of recurrent and metastatic urachal cancer is extremely poor because there is no established chemotherapy regimen. Here, the response of one patient with recurrent urachal cancer to combination chemotherapy of gemcitabi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-06-01 00:00:00
abstract::The overexpression of p53, c-erbB-2 and p21ras gene products was evaluated immunohistochemically in ovarian carcinomas, borderline, and benign neoplasms. All studies were performed on cytospin preparations of cyst and/or ascitic fluid cells and mutual relations between oncoproteins were analysed. p53 and c-erbB-2 immu...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
abstract::The major purpose of this study was to define if the immunosuppressive effect of a transforming growth factor-beta (TGF-beta)-producing autologous tumor vaccine can be abrogated and rendered immunogenic by suppressing its TGF-beta secretion with antisense strategy. In this study, using a TGF-beta antisense gene modifi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::High-performance liquid chromatography revealed that sodium 5,6-benzylidene-L-ascorbate (SBA), dissolved in distilled water, was gradually decomposed into ascorbic acid and benzaldehyde. Among these three compounds, ascorbic acid showed the most potent cytotoxic activity. The cytotoxic activity of each compound was si...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract:BACKGROUND/AIM:Insulin-like growth factor II mRNA-binding protein 3 (IMP3) plays an important role in the adhesion, invasion, and metastasis of tumor cells. Although emerging evidence suggests that IMP3 promotes tumor progression in several malignancies, the expression of IMP3 and its prognostic implication in adenocar...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13683
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND/AIM:The objective of this phase I study was to determine the maximum-tolerated dose (MTD) and recommended dose (RD) of combination therapy with weekly nanoparticle albumin-bound paclitaxel (nab-paclitaxel) and cyclophosphamide (CPA) in metastatic breast cancer (MBC) patients. PATIENTS AND METHODS:Five patie...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13910
更新日期:2019-12-01 00:00:00
abstract::Adult lymphoblastic lymphoma (LBL) can be of T-cell or B-cell lineage. However, the clinical significance of immunophenotypes is largely unknown. We conducted a retrospective study to compare T-cell LBL with its B-cell counterpart. Between 1983 and 1995, 50 adult patients were diagnosed as LBL at National Taiwan Unive...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1997-05-01 00:00:00
abstract::We carried out a systematic review of new drugs active in non-small cell lung carcinoma (NSCLC). Fifty five phase II and III trials were reviewed (vinorelbine (19 trials), paclitaxel (15), gemcitabine (11), docetaxel (6), topotecan (2) or irinotecan (2)). The first four ones could be considered as active drugs when gi...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:1999-09-01 00:00:00
abstract:BACKGROUND:Multidrug resistance (MDR) is one of the major obstacles limiting the efficacy of cancer chemotherapy. Through screening a series of natural products, we have previously identified six naturally occurring bisbenzylisoquinoline alkaloids (BBIs) that possess potent activity to reverse P-glycoprotein (gp)-media...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2005-05-01 00:00:00
abstract::Glioblastomas are the most frequent and most malignant astrocytic gliomas. The epidermal growth factor receptor (EGFR) gene is the most frequently amplified and overexpressed in glioblastomas. The expression of bcl-2 and Bax in EGFR-antisense transfected and un-transfected glioblastoma cell line, U87E and U87V was stu...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract:BACKGROUND:Cyclin D1 plays an important role in regulating the progression of cells through the G1-phase of the cell cycle. The aim of the study was to investigate the expression of cyclin D1 and Ki-67 in squamous cell carcinomas (SCC) and in some premalignant lesions of the penis and to correlate it with clinicopathol...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2007-07-01 00:00:00
abstract:AIM:To evaluate the efficacy of prophylactic radiotherapy at intervention sites in patients with malignant pleural mesothelioma (MPM). PATIENTS AND METHODS:From 05/2010 to 12/2014, 53 patients with histologically confirmed MPM were treated in order to prevent interventional site metastases. Irradiation was carried out...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND:DeltaNp63 is an isoform of the p53 homolog p63, which lacks an amino-terminal transactivation domain. The aim of this study was to detect the deltaNp63 expression in the squamous carcinoma component of adenosquamous carcinoma and evaluate its usefulness as a specific squamous carcinoma marker. PATIENTS AND ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-11-01 00:00:00
abstract::The expression of the protooncogene encoded proteins (c-erbB1, c-erb B2, c-myc, c-fos) and the suppressor gene product p53 was analyzed in 81 human squamous cell carcinomas of the lung and correlated with clinical parameters of the patients (patient survival, presence of metastases and tumor stage) and with biological...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract:AIM:To assess whether prognostic factors in male (MBC) and female (FBC) breast cancer have similar impact on survival. PATIENTS AND METHODS:Charts for men and women diagnosed with breast cancer referred to the London Regional Cancer Program (LRCP) were reviewed. Patients with distant metastatic diseases were excluded....
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2013-05-01 00:00:00