Abstract:
BACKGROUND/AIM:Pyruvate kinase M2 (PKM2) is an enzyme that is predominantly overexpressed in various types of cancer. The role of PKM2 in liver fluke-associated cholangiocarcinoma (CCA) remains unclear. This study aimed to investigate the antitumor activity of shikonin, a PKM2 inhibitor, in CCA cells. MATERIALS AND METHODS:Immunohistochemistry and immunoblotting were used to determine PKM2 expression in CCA tissues and cells. Antiproliferative effects of shikonin were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, colony-formation and trypan blue exclusion assays. The anti-metastatic activity of shikonin was determined using the Boyden chamber assay. Mechanisms by which shikonin inhibited CCA progression were determined. RESULTS:PKM2 was overexpressed in CCA compared to normal bile duct epithelial cells. Shikonin significantly inhibited growth, and migration of CCA cells while inducing their death. A mechanistic study revealed that antitumor effects of shikonin in CCA cells depended on increased production of reactive oxygen species. CONCLUSION:Shikonin may be a novel therapeutic agent for patients with CCA.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Thonsri U,Seubwai W,Waraasawapati S,Wongkham S,Boonmars T,Cha'on U,Wongkham Cdoi
10.21873/anticanres.14515subject
Has Abstractpub_date
2020-09-01 00:00:00pages
5115-5124issue
9eissn
0250-7005issn
1791-7530pii
40/9/5115journal_volume
40pub_type
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journal_title:Anticancer research
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journal_title:Anticancer research
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journal_title:Anticancer research
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doi:
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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journal_title:Anticancer research
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doi:
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doi:
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journal_title:Anticancer research
pub_type: 杂志文章
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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journal_title:Anticancer research
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doi:
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journal_title:Anticancer research
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doi:
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journal_title:Anticancer research
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