Abstract:
:We previously found that human amniotic mesenchymal stem cells (hAMSCs) in coculture with CF immortalised airway epithelial cells (CFBE41o- line, CFBE) on Transwell® filters acquired an epithelial phenotype and led to the expression of a mature and functional CFTR protein. In order to explore the role of gap junction- (GJ-) mediated intercellular communication (GJIC) in this rescue, cocultures (hAMSC : CFBE, 1 : 5 ratio) were studied for the formation of GJIC, before and after silencing connexin 43 (Cx43), a major component of GJs. Functional GJs in cocultures were inhibited when the expression of the Cx43 protein was downregulated. Transfection of cocultures with siRNA against Cx43 resulted in the absence of specific CFTR signal on the apical membrane and reduction in the mature form of CFTR (band C), and in parallel, the CFTR-dependent chloride channel activity was significantly decreased. Cx43 downregulation determined also a decrease in transepithelial resistance and an increase in paracellular permeability as compared with control cocultures, implying that GJIC may regulate CFTR expression and function that in turn modulate airway epithelium tightness. These results indicate that GJIC is involved in the correction of CFTR chloride channel activity upon the acquisition of an epithelial phenotype by hAMSCs in coculture with CF cells.
journal_name
Stem Cells Intjournal_title
Stem cells internationalauthors
Carbone A,Zefferino R,Beccia E,Casavola V,Castellani S,Di Gioia S,Giannone V,Seia M,Angiolillo A,Colombo C,Favia M,Conese Mdoi
10.1155/2018/1203717subject
Has Abstractpub_date
2018-01-11 00:00:00pages
1203717eissn
1687-966Xissn
1687-9678journal_volume
2018pub_type
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journal_title:Stem cells international
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abstract::[This corrects the article DOI: 10.1155/2017/3978595.]. ...
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