Abstract：
:A novel wild-type recombinant cold-active α-d-galactosidase (α-PsGal) from the cold-adapted marine bacterium Pseudoalteromonas sp. KMM 701, and its mutants D451A and C494N, were studied in terms of their structural, physicochemical, and catalytic properties. Homology models of the three-dimensional α-PsGal structure, its active center, and complexes with D-galactose were constructed for identification of functionally important amino acid residues in the active site of the enzyme, using the crystal structure of the α-galactosidase from Lactobacillus acidophilus as a template. The circular dichroism spectra of the wild α-PsGal and mutant C494N were approximately identical. The C494N mutation decreased the efficiency of retaining the affinity of the enzyme to standard p-nitrophenyl-α-galactopiranoside (pNP-α-Gal). Thin-layer chromatography, matrix-assisted laser desorption/ionization mass spectrometry, and nuclear magnetic resonance spectroscopy methods were used to identify transglycosylation products in reaction mixtures. α-PsGal possessed a narrow acceptor specificity. Fructose, xylose, fucose, and glucose were inactive as acceptors in the transglycosylation reaction. α-PsGal synthesized -α(1→6)- and -α(1→4)-linked galactobiosides from melibiose as well as -α(1→6)- and -α(1→3)-linked p-nitrophenyl-digalactosides (Gal₂-pNP) from pNP-α-Gal. The D451A mutation in the active center completely inactivated the enzyme. However, the substitution of C494N discontinued the Gal-α(1→3)-Gal-pNP synthesis and increased the Gal-α(1→4)-Gal yield compared to Gal-α(1→6)-Gal-pNP.
journal_name
Mar Drugsjournal_title
Marine drugsauthors
Bakunina I,Slepchenko L,Anastyuk S,Isakov V,Likhatskaya G,Kim N,Tekutyeva L,Son O,Balabanova Ldoi
10.3390/md16100349subject
Has Abstractpub_date
2018-09-24 00:00:00issue
10issn
1660-3397pii
md16100349journal_volume
16pub_type
杂志文章相关文献
Marine Drugs文献大全abstract：:In recent years, noteworthy research has been performed around lipids from microalgae. Among lipids, glycolipids (GLs) are quite abundant in microalgae and are considered an important source of fatty acids (FAs). GLs are rich in 16- and 18-carbon saturated and unsaturated fatty acids and often contain polyunsaturated ...
journal_title：Marine drugs
pub_type： 杂志文章,评审
doi：10.3390/md14050101
更新日期：2016-05-19 00:00:00
abstract：:Eighteen analogues of the marine cytotoxic linear peptide tasiamide were designed, synthesized and screened for their inhibitory activities against the growth of human nasopharyngeal carcinoma (KB) and human non-small cell lung tumor (A549) cell lines. The results indicated that minor modifications of the C-terminus w...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md12042308
更新日期：2014-04-22 00:00:00
abstract：:Hepatocellular carcinoma (HCC) is a malignant tumor that can cause systemic invasion; however, the exact etiology and molecular mechanism are unknown. Astaxanthin (ASX), a powerful antioxidant, has efficient anti-oxidant, anti-inflammatory, and other activities, and has great research prospects in cancer therapy. We s...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md13106064
更新日期：2015-09-24 00:00:00
abstract：:Nostosins A and B were isolated from a hydrophilic extract of Nostoc sp. strain from Iran, which exhibits excellent tryps inhibitory activity. Nostosin A was the most potent natural tripeptide aldehyde as trypsin inhibitor up to now. Both R- and S-2-hydroxy-4-(4-hydroxy-phenyl) butanoic acid (Hhpba) were prepared and ...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md15030058
更新日期：2017-02-27 00:00:00
abstract：:One of the most distinctive and defining features of coleoid cephalopods-squid, cuttlefish and octopus-is their inking behavior. Their ink, which is blackened by melanin, but also contains other constituents, has been used by humans in various ways for millennia. This review summarizes our current knowledge of cephalo...
journal_title：Marine drugs
pub_type： 杂志文章,评审
doi：10.3390/md12052700
更新日期：2014-05-12 00:00:00
abstract：:Four new sesquiterpene lactones (3, 4, 6 and 7) and three known compounds, purpuride (1), berkedrimane B (2) and purpuride B (5), were isolated from the marine fungus, Talaromyces minioluteus (Penicillium minioluteum). New compounds were drimane sesquiterpenes conjugated with N-acetyl-l-valine, and their structures we...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md13063567
更新日期：2015-06-05 00:00:00
abstract：:The red seaweed Pyropia yezoensis is an ideal research model for dissecting the molecular mechanisms underlying its robust acclimation to abiotic stresses in intertidal zones. Glycine betaine (GB) was an important osmolyte in maintaining osmotic balance and stabilizing the quaternary structure of complex proteins unde...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md17010070
更新日期：2019-01-21 00:00:00
abstract：:Chitosan is sensitive to environmental pH values due to its electric property. This study investigates whether the pH-responsive chitosan assay can provide a simple method to evaluate the aggressive behavior of cancer cells with cell detachment ratio. The epithelial-mesenchymal transition (EMT) is induced with transfo...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md17120659
更新日期：2019-11-23 00:00:00
abstract：:Sea cucumbers belonging to echinoderm are traditionally used as tonic food in China and other Asian countries. They produce abundant biologically active triterpene glycosides. More than 300 triterpene glycosides have been isolated and characterized from various species of sea cucumbers, which are classified as holosta...
journal_title：Marine drugs
pub_type： 杂志文章,评审
doi：10.3390/md15100317
更新日期：2017-10-17 00:00:00
abstract：:Pectenotoxin-2 (PTX-2), which was first identified as a cytotoxic entity in marine sponges, has been reported to display significant cytotoxicity to human cancer cells where it inhibits mitotic separation and cytokinesis through the depolymerization of actin filaments. In the late stage of endoreduplication, the effec...
journal_title：Marine drugs
pub_type： 杂志文章,评审
doi：10.3390/md9112176
更新日期：2011-01-01 00:00:00
abstract：:Bis(2,3-dibromo-4,5-dihydroxybenzyl) ether (BDDE) is a bromophenol isolated from marine algae. Previous reports have shown that BDDE possesses cytotoxic and antibacterial activity. In the present study, we demonstrate that BDDE displays broad-spectrum antifungal activities, especially on Botrytis cinerea. BDDE inhibit...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md12073838
更新日期：2014-06-27 00:00:00
abstract：:Gliotoxin, a secondary metabolite produced by marine fungus Aspergillus sp., possesses various biological activities including anticancer activity. However, the mechanism underlying gliotoxin-induced cytotoxicity on human cervical cancer (Hela) and human chondrosarcoma (SW1353) cells remains unclear. In this study, we...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md12010069
更新日期：2013-12-24 00:00:00
abstract：:Bone defects in human, caused by fractures/nonunions or trauma, gain increasing impact and have become a medical challenge in the present-day aging population. Frequently, those fractures require surgical intervention which ideally relies on autografts or suboptimally on allografts. Therefore, it is pressing and likew...
journal_title：Marine drugs
pub_type： 杂志文章,评审
doi：10.3390/md11030718
更新日期：2013-03-08 00:00:00
abstract：:The red seaweeds belonging to the genus Laurencia are well known as halogenated secondary metabolites producers, mainly terpenoids and acetogennins. Several of these chemicals exhibit important ecological roles and biotechnological applications. However, knowledge regarding the genes involved in the biosynthesis of th...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md13020879
更新日期：2015-02-10 00:00:00
abstract：:Astaxanthin (AXT), a xanthophyll carotenoid compound, has potent antioxidant, anti-inflammatory and neuroprotective properties. Neuroinflammation and oxidative stress are significant in the pathogenesis and development of Alzheimer's disease (AD). Here, we studied whether AXT could alleviate neuroinflammation, oxidati...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md17020123
更新日期：2019-02-18 00:00:00
abstract：:Over the past decades, a number of novel compounds, which are produced in the marine environment, have been found to exhibit the anticancer effects. This review focuses on molecular targets of marine-derived anticancer candidates in clinical and preclinical studies. They are kinases, transcription factors, histone dea...
journal_title：Marine drugs
pub_type： 杂志文章,评审
doi：10.3390/md16050175
更新日期：2018-05-22 00:00:00
abstract：:Pinnatoxins (PnTXs) are emerging neurotoxins that were discovered about 30 years ago. They are solely produced by the marine dinoflagellate Vulcanodinium rugosum, and may be transferred into the food chain, as they have been found in various marine invertebrates, including bivalves. No human intoxication has been repo...
journal_title：Marine drugs
pub_type： 杂志文章,评审
doi：10.3390/md17070425
更新日期：2019-07-20 00:00:00
abstract：:Marine sponges belonging to the phylum Porifera (Metazoa), evolutionarily the oldest animals are the single best source of marine natural products. The present review presents a comprehensive overview of the source, taxonomy, country of origin or geographical position, chemical class, and biological activity of sponge...
journal_title：Marine drugs
pub_type： 杂志文章,评审
doi：10.3390/md12084539
更新日期：2014-08-19 00:00:00
abstract：:Notably, α-conotoxins with carboxy-terminal (C-terminal) amidation are inhibitors of the pentameric nicotinic acetylcholine receptors (nAChRs), which are therapeutic targets for neurological diseases and disorders. The (α3)2(β2)3 nAChR subunit arrangement comprises a pair of α3(+)β2(-) and β2(+)α3(-) interfaces, and a...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md18070349
更新日期：2020-07-03 00:00:00
abstract：:Fish consumption is a potential route of human exposure to the hepatotoxic microcystins, especially in lakes and reservoirs that routinely experience significant toxic Microcystis blooms. Understanding the rates of uptake and elimination for microcystins as well as the transfer efficiency into tissues of consumers are...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md9122553
更新日期：2011-12-01 00:00:00
abstract：:Drug-resistant Staphylococcus aureus is a continuing public health concern, both in the hospital and community settings. Antibacterial compounds that possess novel structural scaffolds and are effective against multiple S. aureus strains, including current drug-resistant ones, are needed. Previously, we have described...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md10051103
更新日期：2012-05-01 00:00:00
abstract：:Prostate cancer (PCa), the most common malignancy in men, is a major cause of cancer deaths. A better understanding of the mechanisms that drive tumor initiation and progression may identify actionable targets to improve treatment of this patient group. As a dietary carotenoid, astaxanthin has been demonstrated to exe...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md15030066
更新日期：2017-03-08 00:00:00
abstract：:Water-soluble sulfated polysaccharides isolated from two red algae Sphaerococcus coronopifolius (Gigartinales, Sphaerococcaceae) and Boergeseniella thuyoides (Ceramiales, Rhodomelaceae) collected on the coast of Morocco inhibited in vitro replication of the Human Immunodeficiency Virus (HIV) at 12.5 μg/mL. In addition...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md9071187
更新日期：2011-01-01 00:00:00
abstract：:The marine nemertean Cephalothrix simula originates from the Pacific Ocean but in recent years has been discovered in northern Europe. The species has been associated with high levels of the marine neurotoxin Tetrodotoxin, traditionally associated with Pufferfish Poisoning. This study reports the first discovery of tw...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md16110452
更新日期：2018-11-16 00:00:00
abstract：:Sponges are important sources of bioactive secondary metabolites. These compounds are frequently synthesized by bacterial symbionts, which may be recruited from the surrounding seawater or transferred to the sponge progeny by the parent. In this study, we investigated the bacterial communities associated with the spon...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md15040095
更新日期：2017-03-25 00:00:00
abstract：:The antiproliferative activity of 52 volatile compounds released from bacteria was investigated in agar diffusion assays against medically important microorganisms and mouse fibroblasts. Furthermore, the activity of these compounds to interfere with the quorum-sensing-systems was tested with two different reporter str...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md8122976
更新日期：2010-12-22 00:00:00
abstract：:Two new indole-diterpenoids (1 and 2) and a new isocoumarin (3), along with the known β-aflatrem (4), paspalinine (5), leporin B (6), α-cyclopiazonic acid (7), iso-α-cyclopiazonic acid (8), ditryptophenaline (9), aflatoxin B1 (10), 7-O-acetylkojic acid (11) and kojic acid (12), were isolated from the fermentation brot...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md12073970
更新日期：2014-06-30 00:00:00
abstract：:Conotoxins in the venom of cone snails (Conus spp.) are a mixture of active peptides that work as blockers, agonists, antagonists, or inactivators of various ion channels. Recently we reported a high-throughput method to identify 215 conotoxin transcripts from the Chinese tubular cone snail, C. betulinus. Here, based ...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md15070228
更新日期：2017-07-18 00:00:00
abstract：:The democratization of sequencing technologies fostered a leap in our knowledge of the diversity of marine phytoplanktonic microalgae, revealing many previously unknown species and lineages. The evolutionary history of the diversification of microalgae can be inferred from the analysis of their genome sequences. Howev...
journal_title：Marine drugs
pub_type： 杂志文章
doi：10.3390/md18020078
更新日期：2020-01-25 00:00:00
abstract：:The authors wish to correct Figure 1 in this paper [1] to be as follows:[...]. ...
journal_title：Marine drugs
pub_type： 杂志文章,已发布勘误
doi：10.3390/md15090292
更新日期：2017-09-20 00:00:00
