Characterization of Properties and Transglycosylation Abilities of Recombinant α-Galactosidase from Cold-Adapted Marine Bacterium Pseudoalteromonas KMM 701 and Its C494N and D451A Mutants.


:A novel wild-type recombinant cold-active α-d-galactosidase (α-PsGal) from the cold-adapted marine bacterium Pseudoalteromonas sp. KMM 701, and its mutants D451A and C494N, were studied in terms of their structural, physicochemical, and catalytic properties. Homology models of the three-dimensional α-PsGal structure, its active center, and complexes with D-galactose were constructed for identification of functionally important amino acid residues in the active site of the enzyme, using the crystal structure of the α-galactosidase from Lactobacillus acidophilus as a template. The circular dichroism spectra of the wild α-PsGal and mutant C494N were approximately identical. The C494N mutation decreased the efficiency of retaining the affinity of the enzyme to standard p-nitrophenyl-α-galactopiranoside (pNP-α-Gal). Thin-layer chromatography, matrix-assisted laser desorption/ionization mass spectrometry, and nuclear magnetic resonance spectroscopy methods were used to identify transglycosylation products in reaction mixtures. α-PsGal possessed a narrow acceptor specificity. Fructose, xylose, fucose, and glucose were inactive as acceptors in the transglycosylation reaction. α-PsGal synthesized -α(1→6)- and -α(1→4)-linked galactobiosides from melibiose as well as -α(1→6)- and -α(1→3)-linked p-nitrophenyl-digalactosides (Gal₂-pNP) from pNP-α-Gal. The D451A mutation in the active center completely inactivated the enzyme. However, the substitution of C494N discontinued the Gal-α(1→3)-Gal-pNP synthesis and increased the Gal-α(1→4)-Gal yield compared to Gal-α(1→6)-Gal-pNP.


Mar Drugs


Marine drugs


Bakunina I,Slepchenko L,Anastyuk S,Isakov V,Likhatskaya G,Kim N,Tekutyeva L,Son O,Balabanova L




Has Abstract


2018-09-24 00:00:00










  • The deep-sea natural products, biogenic polyphosphate (Bio-PolyP) and biogenic silica (Bio-Silica), as biomimetic scaffolds for bone tissue engineering: fabrication of a morphogenetically-active polymer.

    abstract::Bone defects in human, caused by fractures/nonunions or trauma, gain increasing impact and have become a medical challenge in the present-day aging population. Frequently, those fractures require surgical intervention which ideally relies on autografts or suboptimally on allografts. Therefore, it is pressing and likew...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Wang X,Schröder HC,Feng Q,Draenert F,Müller WE

    更新日期:2013-03-08 00:00:00

  • Understanding nanocalcification: a role suggested for crystal ghosts.

    abstract::The present survey deals with the initial stage of the calcification process in bone and other hard tissues, with special reference to the organic-inorganic relationship and the transformation that the early inorganic particles undergo as the process moves towards completion. Electron microscope studies clearly exclud...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Bonucci E

    更新日期:2014-07-23 00:00:00

  • Marine Natural Products from the Yucatan Peninsula.

    abstract::Mexico is one of the three areas of the world with the greatest terrestrial and cultural biological diversity. The diversity of Mexican medicinal flora has been studied for a long time and several bioactive compounds have been isolated. The investigation of marine resources, and particularly the potential of Mexican m...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Pech-Puch D,Pérez-Povedano M,Lenis-Rojas OA,Rodríguez J,Jiménez C

    更新日期:2020-01-16 00:00:00

  • Sphaeroane and neodolabellane diterpenes from the red alga Sphaerococcus coronopifolius.

    abstract::Investigation of minor metabolites in the extracts of the red alga Sphaerococcus coronopifolius collected from the rocky coasts of Corfu Island in the Ionian Sea yielded two new diterpene alcohols, sphaerollanes I, and II (1, 2) featuring neodolabellane skeletons, and the new sphaeroane diterpene alcohol 16-hydroxy-9S...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Smyrniotopoulos V,Vagias C,Roussis V

    更新日期:2009-05-19 00:00:00

  • Development of pedigree classification using microsatellite and mitochondrial markers for Giant grouper broodstock (Epinephelus lanceolatus) management in Taiwan.

    abstract::Most giant groupers in the market are derived from inbred stock. Inbreeding can cause trait depression, compromising the animals' fitness and disease resistance, obligating farmers to apply increased amounts of drugs. In order to solve this problem, a pedigree classification method is needed. Here, microsatellite and ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Kuo HC,Hsu HH,Chua CS,Wang TY,Chen YM,Chen TY

    更新日期:2014-04-30 00:00:00

  • Spirulina maxima Derived Pectin Nanoparticles Enhance the Immunomodulation, Stress Tolerance, and Wound Healing in Zebrafish.

    abstract::In this study, Spirulina maxima derived pectin nanoparticles (SmPNPs) were synthesized and multiple biological effects were investigated using in vitro and in vivo models. SmPNPs were not toxic to Raw 264.7 cells and zebrafish embryos up to 1 mg/mL and 200 µg/mL, respectively. SmPNPs upregulated Il 10, Cat, Sod 2, Def...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Rajapaksha DC,Edirisinghe SL,Nikapitiya C,Dananjaya S,Kwun HJ,Kim CH,Oh C,Kang DH,De Zoysa M

    更新日期:2020-11-07 00:00:00

  • A Low Molecular Weight Protein from the Sea Anemone Anemonia viridis with an Anti-Angiogenic Activity.

    abstract::Sea anemones are a remarkable source of active principles due to a decentralized venom system. New blood vessel growth or angiogenesis is a very promising target against cancer, but the few available antiangiogenic compounds have limited efficacy. In this study, a protein fraction, purified from tentacles of Anemonia ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Loret EP,Luis J,Nuccio C,Villard C,Mansuelle P,Lebrun R,Villard PH

    更新日期:2018-04-19 00:00:00

  • Ciguatoxins Evoke Potent CGRP Release by Activation of Voltage-Gated Sodium Channel Subtypes NaV1.9, NaV1.7 and NaV1.1.

    abstract::Ciguatoxins (CTXs) are marine toxins that cause ciguatera fish poisoning, a debilitating disease dominated by sensory and neurological disturbances that include cold allodynia and various painful symptoms as well as long-lasting pruritus. Although CTXs are known as the most potent mammalian sodium channel activator to...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Touska F,Sattler S,Malsch P,Lewis RJ,Reeh PW,Zimmermann K

    更新日期:2017-08-30 00:00:00

  • Anticancer Activity of Fascaplysin against Lung Cancer Cell and Small Cell Lung Cancer Circulating Tumor Cell Lines.

    abstract::Lung cancer is a leading cause of tumor-associated mortality. Fascaplysin, a bis-indole of a marine sponge, exhibit broad anticancer activity as specific CDK4 inhibitor among several other mechanisms, and is investigated as a drug to overcome chemoresistance after the failure of targeted agents or immunotherapy. The c...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Rath B,Hochmair M,Plangger A,Hamilton G

    更新日期:2018-10-14 00:00:00

  • The antihyperlipidemic mechanism of high sulfate content ulvan in rats.

    abstract::Numerous studies have suggested that hyperlipidemia is closely linked to cardiovascular disease. The aim of this study was to investigate the possible antihyperlipidemia mechanism of HU (high sulfate content of ulvan) in high-cholesterol fed rats. Wistar rats were made hyperlipidemic by feeding with a high-cholesterol...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Qi H,Sheng J

    更新日期:2015-05-29 00:00:00

  • Marine Bacteria, A Source for Alginolytic Enzyme to Disrupt Pseudomonas aeruginosa Biofilms.

    abstract::Pseudomonas aeruginosa biofilms are typically associated with the chronic lung infection of cystic fibrosis (CF) patients and represent a major challenge for treatment. This opportunistic bacterial pathogen secretes alginate, a polysaccharide that is one of the main components of its biofilm. Targeting this major biof...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Daboor SM,Raudonis R,Cohen A,Rohde JR,Cheng Z

    更新日期:2019-05-24 00:00:00

  • Bioactive cembrane-based diterpenoids from the soft coral Sinularia triangular.

    abstract::Chemical examination of the Taiwanese soft coral Sinularia triangular led to the isolation of five cembrane-based diterpenoids 1-5, including two new metabolites, triangulenes A (1) and B (2). The structures of the new metabolites were determined on the basis of extensive spectroscopic analysis, particularly mass spec...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Su JH,Wen ZH

    更新日期:2011-01-01 00:00:00

  • Production, characterization and biocompatibility of marine collagen matrices from an alternative and sustainable source: the sea urchin Paracentrotus lividus.

    abstract::Collagen has become a key-molecule in cell culture studies and in the tissue engineering field. Industrially, the principal sources of collagen are calf skin and bones which, however, could be associated to risks of serious disease transmission. In fact, collagen derived from alternative and riskless sources is requir...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Benedetto CD,Barbaglio A,Martinello T,Alongi V,Fassini D,Cullorà E,Patruno M,Bonasoro F,Barbosa MA,Carnevali MD,Sugni M

    更新日期:2014-09-24 00:00:00

  • Gliotoxin Inhibits Proliferation and Induces Apoptosis in Colorectal Cancer Cells.

    abstract::The discovery of new bioactive compounds from marine natural sources is very important in pharmacological research. Here we developed a Wnt responsive luciferase reporter assay to screen small molecule inhibitors of cancer associated constitutive Wnt signaling pathway. We identified that gliotoxin (GTX) and some of it...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Chen J,Wang C,Lan W,Huang C,Lin M,Wang Z,Liang W,Iwamoto A,Yang X,Liu H

    更新日期:2015-10-02 00:00:00

  • Ubiquitin-Proteasome Modulating Dolabellanes and Secosteroids from Soft Coral Clavularia flava.

    abstract::We performed a high-content screening (HCS) assay aiming to discover bioactive molecules with proteasome inhibitory activity. By structural elucidation, we identified six compounds purified from soft coral Clavularia flava, which potentiates proteasome inhibition. Chemical structure elucidation revealed they are dolab...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Chiu CY,Ling XH,Wang SK,Duh CY

    更新日期:2020-01-03 00:00:00

  • Ochracenoids A and B, guaiazulene-based analogues from gorgonian Anthogorgia ochracea collected from the South China Sea.

    abstract::Two new guaiazulene-based analogues, ochracenoids A (1) and B (2), along with four known analogues (3-6), were isolated from the gorgonian Anthogorgia ochracea collected from the South China Sea. The planar structures of the new compounds were elucidated by comprehensive spectroscopic data. The absolute configuration ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Zheng JJ,Shao CL,Chen M,Gan LS,Fang YC,Wang XH,Wang CY

    更新日期:2014-03-14 00:00:00

  • Alkaloids from the mangrove-derived actinomycete Jishengella endophytica 161111.

    abstract::A new alkaloid, 2-(furan-2-yl)-6-(2S,3S,4-trihydroxybutyl)pyrazine (1), along with 12 known compounds, 2-(furan-2-yl)-5-(2S,3S,4-trihydroxybutyl)pyrazine (2), (S)-4-isobutyl-3-oxo-3,4-dihydro-1H-pyrrolo[2,1-c][1,4]oxazine-6-carbaldehyde (3), (S)-4-isopropyl-3-oxo-3,4-dihydro-1H-pyrrolo[2,1-c][1,4]oxazine-6-carbaldehyd...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Wang P,Kong F,Wei J,Wang Y,Wang W,Hong K,Zhu W

    更新日期:2014-01-21 00:00:00

  • Viridicatol Isolated from Deep-Sea Penicillium Griseofulvum Alleviates Anaphylaxis and Repairs the Intestinal Barrier in Mice by Suppressing Mast Cell Activation.

    abstract::Viridicatol is a quinoline alkaloid isolated from the deep-sea-derived fungus Penicillium griseofulvum. The structure of viridicatol was unambiguously established by X-ray diffraction analysis. In this study, a mouse model of ovalbumin-induced food allergy and the rat basophil leukemia (RBL)-2H3 cell model were establ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Shu Z,Liu Q,Xing C,Zhang Y,Zhou Y,Zhang J,Liu H,Cao M,Yang X,Liu G

    更新日期:2020-10-16 00:00:00

  • Targets and effects of yessotoxin, okadaic acid and palytoxin: a differential review.

    abstract::In this review, we focus on processes, organs and systems targeted by the marine toxins yessotoxin (YTX), okadaic acid (OA) and palytoxin (PTX). The effects of YTX and their basis are analyzed from data collected in the mollusc Mytilus galloprovincialis, the annelid Enchytraeus crypticus, Swiss CD1 mice and invertebra...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Franchini A,Malagoli D,Ottaviani E

    更新日期:2010-03-16 00:00:00

  • New Eunicellin-Type Diterpenes from the Panamanian Octocoral Briareum Asbestinum.

    abstract::Gorgonian octocorals are considered a prolific source of secondary metabolites with a wide range of biological activities, including anti-inflammatory activity. In particular, the genus Briareum is known for producing a wealth of diterpenes with complex chemical structures. The chemical study of the methanolic extract...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Gutiérrez M,Santamaría R,Gómez-Reyes JF,Guzmán HM,Ávila-Román J,Motilva V,Talero E

    更新日期:2020-01-27 00:00:00

  • Isolation of a new natural product and cytotoxic and antimicrobial activities of extracts from fungi of Indonesian marine habitats.

    abstract::In the search for bioactive compounds, 11 fungal strains were isolated from Indonesian marine habitats. Ethyl acetate extracts of their culture broth were tested for cytotoxic activity against a urinary bladder carcinoma cell line and for antifungal and antibacterial activities against fish and human pathogenic bacter...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Tarman K,Lindequist U,Wende K,Porzel A,Arnold N,Wessjohann LA

    更新日期:2011-02-25 00:00:00

  • Bacterial Long-Chain Polyunsaturated Fatty Acids: Their Biosynthetic Genes, Functions, and Practical Use.

    abstract::The nutritional and pharmaceutical values of long-chain polyunsaturated fatty acids (LC-PUFAs) such as arachidonic, eicosapentaenoic and docosahexaenoic acids have been well recognized. These LC-PUFAs are physiologically important compounds in bacteria and eukaryotes. Although little is known about the biosynthetic me...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Yoshida K,Hashimoto M,Hori R,Adachi T,Okuyama H,Orikasa Y,Nagamine T,Shimizu S,Ueno A,Morita N

    更新日期:2016-05-12 00:00:00

  • Functional Characterization and Evolutionary Analysis of Glycine-Betaine Biosynthesis Pathway in Red Seaweed Pyropia yezoensis.

    abstract::The red seaweed Pyropia yezoensis is an ideal research model for dissecting the molecular mechanisms underlying its robust acclimation to abiotic stresses in intertidal zones. Glycine betaine (GB) was an important osmolyte in maintaining osmotic balance and stabilizing the quaternary structure of complex proteins unde...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Mao Y,Chen N,Cao M,Chen R,Guan X,Wang D

    更新日期:2019-01-21 00:00:00

  • Pretrichodermamides D-F from a Marine Algicolous Fungus Penicillium sp. KMM 4672.

    abstract::Three new epidithiodiketopiperazines pretrichodermamides D-F (1-3), together with the known N-methylpretrichodermamide B (4) and pretrichodermamide С (5), were isolated from the lipophilic extract of the marine algae-derived fungus Penicillium sp. KMM 4672. The structures of compounds 1-5 were determined based on spec...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Yurchenko AN,Smetanina OF,Ivanets EV,Kalinovsky AI,Khudyakova YV,Kirichuk NN,Popov RS,Bokemeyer C,von Amsberg G,Chingizova EA,Afiyatullov SSh,Dyshlovoy SA

    更新日期:2016-06-27 00:00:00

  • Reclamation of Marine Chitinous Materials for the Production of α-Glucosidase Inhibitors via Microbial Conversion.

    abstract::Six kinds of chitinous materials have been used as sole carbon/nitrogen (C/N) sources for producing α-glucosidase inhibitors (aGI) by Paenibacillus sp. TKU042. The aGI productivity was found to be highest in the culture supernatants using demineralized crab shell powder (deCSP) and demineralized shrimp shell powder (d...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Nguyen VB,Wang SL

    更新日期:2017-11-07 00:00:00

  • Combination of trabectedin and gemcitabine for advanced soft tissue sarcomas: results of a phase I dose escalating trial of the German Interdisciplinary Sarcoma Group (GISG).

    abstract:BACKGROUND:Evaluation of the potential efficacy and safety of combination therapies for advanced soft tissue sarcomas (STS) has increased substantially after approval of trabectedin and pazopanib. Trabectedin's introduction in Europe in 2007 depended mainly on its activity in so-called L-sarcomas (liposarcoma and leiom...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Kasper B,Reichardt P,Pink D,Sommer M,Mathew M,Rauch G,Hohenberger P

    更新日期:2015-01-13 00:00:00

  • Cultivation of Sponge-Associated Bacteria from Agelas sventres and Xestospongia muta Collected from Different Depths.

    abstract::Sponge-associated bacteria have been mostly cultured from shallow water (≤30 m) sponges, whereas only few studies targeted specimens from below 30 m. This study assessed the cultivability of bacteria from two marine sponges Xestospongia muta and Agelas sventres collected from shallow (<30 m), upper mesophotic (30-60 m...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Indraningrat AAG,Micheller S,Runderkamp M,Sauerland I,Becking LE,Smidt H,Sipkema D

    更新日期:2019-10-11 00:00:00

  • Astaxanthin Ameliorates Lipopolysaccharide-Induced Neuroinflammation, Oxidative Stress and Memory Dysfunction through Inactivation of the Signal Transducer and Activator of Transcription 3 Pathway.

    abstract::Astaxanthin (AXT), a xanthophyll carotenoid compound, has potent antioxidant, anti-inflammatory and neuroprotective properties. Neuroinflammation and oxidative stress are significant in the pathogenesis and development of Alzheimer's disease (AD). Here, we studied whether AXT could alleviate neuroinflammation, oxidati...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Han JH,Lee YS,Im JH,Ham YW,Lee HP,Han SB,Hong JT

    更新日期:2019-02-18 00:00:00

  • Biotransfer of β-N-methylamino-L-alanine (BMAA) in a eutrophicated freshwater lake.

    abstract::β-N-Methylamino-L-alanine (BMAA), a neurotoxic non-protein amino acid, plays a significant role as an environmental risk factor in neurodegenerative diseases, such as amyotrophic lateral sclerosis. BMAA producers occur globally, colonizing almost all habitats and represent species from distinct phytoplanktonic groups,...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Lage S,Annadotter H,Rasmussen U,Rydberg S

    更新日期:2015-03-02 00:00:00

  • Recently confirmed apoptosis-inducing lead compounds isolated from marine sponge of potential relevance in cancer treatment.

    abstract::Despite intense efforts to develop non-cytotoxic anticancer treatments, effective agents are still not available. Therefore, novel apoptosis-inducing drug leads that may be developed into effective targeted cancer therapies are of interest to the cancer research community. Targeted cancer therapies affect specific abe...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Essack M,Bajic VB,Archer JA

    更新日期:2011-01-01 00:00:00