Abstract:
:In the past decades, advances in microscopy have made it possible to study the dynamics of individual biomolecules in vitro and resolve intramolecular kinetics that would otherwise be hidden in ensemble averages. More recently, single-molecule methods have been used to image, localize, and track individually labeled macromolecules in the cytoplasm of living cells, allowing investigations of intermolecular kinetics under physiologically relevant conditions. In this review, we illuminate the particular advantages of single-molecule techniques when studying kinetics in living cells and discuss solutions to specific challenges associated with these methods.
journal_name
Annu Rev Biochemjournal_title
Annual review of biochemistryauthors
Elf J,Barkefors Idoi
10.1146/annurev-biochem-013118-110801subject
Has Abstractpub_date
2019-06-20 00:00:00pages
635-659eissn
0066-4154issn
1545-4509journal_volume
88pub_type
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