Abstract:
:Genetic, biochemical, and spectroscopic studies have established a new function for an intracellular protein, i.e., guiding and inserting a copper cofactor into the active site of a target enzyme. Studies of these new proteins have revealed a fundamental aspect of copper physiology, namely the vast overcapacity of the cytoplasm for copper sequestration. This finding framed the mechanistic, energetic, and structural aspects of intracellular copper trafficking proteins. One hallmark of the copper chaperones is the similarity of the protein fold between the chaperone and its target enzyme. The surface residues presented by each partner, however, are quite different, and some initial findings concerning the complementarity of these interfaces have led to mechanistic insights. The copper chaperones appear to lower the activation barrier for metal transfer into specific protein-binding sites. The manner in which they facilitate metal insertion appears to involve a docking of the metal donor and acceptor sites in close proximity to one another. Although the intimate mechanism is still open, it appears that a low activation barrier for metal transfer is achieved by a network of coordinate-covalent, electrostatic, and hydrogen bonding interactions in the vicinity of the metal-binding site itself.
journal_name
Annu Rev Biochemjournal_title
Annual review of biochemistryauthors
Huffman DL,O'Halloran TVdoi
10.1146/annurev.biochem.70.1.677subject
Has Abstractpub_date
2001-01-01 00:00:00pages
677-701eissn
0066-4154issn
1545-4509pii
70/1/677journal_volume
70pub_type
杂志文章,评审abstract::This review focuses on eukaryotic translesion synthesis (TLS) DNA polymerases, and the emphasis is on Saccharomyces cerevisiae and human Y-family polymerases (Pols) eta, iota, kappa, and Rev1, as well as on Polzeta, which is a member of the B-family polymerases. The fidelity, mismatch extension ability, and lesion byp...
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更新日期:1999-01-01 00:00:00
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更新日期:1992-01-01 00:00:00
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journal_title:Annual review of biochemistry
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更新日期:2020-06-20 00:00:00