Abstract:
AIM:Ischemia/reperfusion (I/R) injury is the major cause of neurological deficit following stroke. Our previous study showed neuroprotective effects of hispidulin against cerebral ischemia reperfusion injury (IRI). In this study, we further examined the involvement of pyroptosis in this neuroprotective function. MATERIALS AND METHODS:IRI was simulated in a rat model by middle cerebral artery occlusion (MCAO) surgery, and the animals were treated with different doses of hispidulin. The neurological function of the rats was evaluated by the neural function defect score (NFDS), balance beam test and limb placement test. The infarct volume and brain water content were measured 72 h following IRI. Neuronal cell survival and pyroptosis in the ischemic cortex were respectively detected by Nissl staining and TUNEL assay. The relative expression of pyroptosis markers was determined by qRT-PCR, Western blotting and ELISA as appropriate. IRI was simulated in vitro in primary cerebral astrocytes using the OGD/R procedure. AMPKα was blocked genetically or pharmacologically using siRNA and compound C respectively. CCK-8 and LDH release assays were performed using suitable kits. RESULTS:Hispidulin improved the neurological symptoms of the rats after IRI, in addition to decreasing the infarct size and brain edema. Mechanistically, hispidulin exerted its neuroprotective effects in vivo and in vitro by suppressing NLRP3-mediated pyroptosis by modulating the AMPK/GSK3β signaling pathway. CONCLUSION:Hispidulin is a neuroprotective agent with clinical potential against IR-induced neurological injury.
journal_name
Life Scijournal_title
Life sciencesauthors
An P,Xie J,Qiu S,Liu Y,Wang J,Xiu X,Li L,Tang Mdoi
10.1016/j.lfs.2019.116599subject
Has Abstractpub_date
2019-09-01 00:00:00pages
116599eissn
0024-3205issn
1879-0631pii
S0024-3205(19)30525-9journal_volume
232pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::Calcium ionophores, such as the A23187, cause endothelium-dependent relaxation of arterial strips with intact endothelium, whereas the effect of the ionophore should result from the combination of a relaxation caused by the endothelium-dependent factors and of a contraction of the smooth muscles. In addition, the appl...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00646-9
更新日期:2000-06-23 00:00:00
abstract::Thymic hormones have immunomodulatory effects on T cells and hence have been used clinically to restore the immunity of immunodeficient patients as well as to enhance the cellular immunity of cancer patients. Prothymosin alpha, which is a member of the thymic hormone family, has recently been suggested to act as a nuc...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)00882-5
更新日期:1997-01-01 00:00:00
abstract::The effect of maternal glucocorticoid depletion upon the fetal development of the organ of Zuckerkandl (OZ) in rats was determined. Maternal hypophysectomy at 13d8h gestation resulted in a fifty percent decrease in plasma corticosterone levels at 18d8h when compared to both sham operated and unoperated controls. No di...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90609-6
更新日期:1987-09-14 00:00:00
abstract::The accumulation of lysophosphoglycerides has been implicated as an important biochemical factor for cardiac arrhythmias. Recently, we demonstrated that lysophosphatidylcholine caused cardiac arrhythmias in the isolated hamster heart. In this study, the arrhythmogenic nature of various lysophosphoglycerides with respe...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90806-8
更新日期:1983-03-21 00:00:00
abstract::The effects of the novel anticonvulsant felbamate, which binds to the 5-7 dichlorokynurenic binding sites, were tested towards the CA1 epileptiform activity induced in rat hippocampal slices by kainic acid. The effects of the kynurenic acid derivatives 7-chlorokynurenic acid and 5-7 dichlorokynurenic acid and of the N...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(96)00251-2
更新日期:1996-05-24 00:00:00
abstract::The effect of intravenous administration of human epidermal growth factor on the splanchnic blood flows was examined in anesthetized dogs, using an ultrasonic transit-time volume flow meter. Human epidermal growth factor (0.1, 0.5 and 1 microgram/kg) significantly increased blood flows in the portal vein (36.9 +/- 7.4...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90170-v
更新日期:1990-01-01 00:00:00
abstract::Two analogues of [Nle28,31]-CCK26-33 containing an N-terminal acetyl or N-terminal hydroxysulfonyl moiety were prepared and characterized. Both analogues were equipotent to native CCK26-33 in four bioassays, demonstrating that N-terminal sulfation of CCK26-33 analogues is compatible with full biological activity. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90158-x
更新日期:1986-11-03 00:00:00
abstract::High salt intake is involved in the genesis of hypertension and vascular changes in salt-sensitive patients. Although many mechanisms have been proposed, the underlying mechanisms of these alterations in healthy rats are not completely elucidated. The aim of this study was to investigate if male Wistar rats fed a high...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.09.028
更新日期:2006-04-04 00:00:00
abstract::Studies were performed to compare in vitro and in vivo effects of morphine on the phagocytic function of murine peritoneal macrophages. Macrophage monolayers were incubated with Candida albicans for 30 min in the absence of autologous serum. Morphine added in vitro was found to decrease both the phagocytic activity (p...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90122-j
更新日期:1993-01-01 00:00:00
abstract::To assess the possible role of altered central serotonin function in psychiatric disorders, investigators have utilized pharmacological challenge testing with an amino acid mixture to decrease blood tryptophan concentration and, indirectly, brain serotonin levels. The aim of this pilot study was to assess the effectiv...
journal_title:Life sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0024-3205(95)00103-4
更新日期:1995-03-17 00:00:00
abstract::There is now some evidence that i) the availability of plasma tryptophan, the precursor of serotonin, is significantly lower in pregnant women at the end of term and the first few days after delivery than in nonpregnant women; and ii) both pregnancy and the early puerperium are accompanied by activation of the inflamm...
journal_title:Life sciences
pub_type: 临床试验,杂志文章
doi:10.1016/s0024-3205(02)01853-2
更新日期:2002-09-06 00:00:00
abstract::Dihydroergosine (50 and 100 mg/kg) enhanced the incidence of bicuculline (3 mg/kg)-induced convulsions in female rats, while 100 mg/kg of dihydroergosine given to female mice made 45% convulsive dose of bicuculline (2.5 mg/kg) to be subconvulsive. The same dose of dihydroergosine enhanced in mice the latency of bicucu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90608-t
更新日期:1990-01-01 00:00:00
abstract::After their discovery, the two known cannabinoid receptors, CB(1) and CB(2), have been the focus of research into the cellular signalling mechanisms of cannabinoids. The initial assessment, mainly derived from expression studies, was that cannabinoids, via G(i/o) proteins, negatively modulate cyclic AMP levels, and ac...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2005.05.055
更新日期:2006-01-02 00:00:00
abstract::This study examined the role of marijuana smoking in the pathogenesis of human lung cancer by measuring DNA damage in alveolar macrophages (AM). The alkaline unwinding method was used to determine DNA single-strand breaks in AM lavaged from non-smokers [NS] and smokers of marijuana [MS], tobacco [TS] or cocaine [CS], ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00208-n
更新日期:1995-01-01 00:00:00
abstract::The effects of isolation and water scheduling on mitogen induced lymphocyte proliferation were investigated. Isolated rats were animals which had been raised in group-housed conditions and then transferred to individual cages with ad lib access to water for a 1 or 2 week period. Water scheduled rats were maintained in...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90472-9
更新日期:1988-01-01 00:00:00
abstract::Calcium spirulan (Ca-SP) is a novel sulfated polysaccharide isolated from a blue-green alga Spirulina platensis. Ca-SP inhibits thrombin by activation of heparin cofactor II. Therefore, it could serve as an origin of anti-atherogenic medicines. Since maintenance of vascular endothelial cell monolayers is important for...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01555-7
更新日期:2002-03-08 00:00:00
abstract::Recent studies have indicated that Ginsenoside Rb1, one of the major components of ginseng root, may play an important role in protecting cells from damage. Here, we investigated the neuroprotective activity of Rb1 after hypoxic injury in young rats. About 50% animals were dead by exposing hypoxic condition three time...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.10.011
更新日期:2005-01-14 00:00:00
abstract:BACKGROUND:Cisplatin resistance has been found to contribute to the failure of ovarian carcinoma treatment. Oridonin is a natural en-kaurane tetracyclic diterpenoid compound discovered in Rabdosia rubescene (Henmsl.) Hara. Herein, we tested whether oridonin could exert chemo-sensitization activity on cisplatin-resistan...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.116709
更新日期:2019-09-15 00:00:00
abstract:AIMS:Fcγ receptor I (FcγRI/CD64) that is restrictedly expressed on monocytes and macrophages, acts as the single high-affinity receptor of immunoglobulin G (IgG) in human. The expression of FcγRI is positively correlated with immune inflammation. The primary aim of this study was to explore the effects of FcγRI express...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.06.015
更新日期:2018-08-15 00:00:00
abstract:AIMS:Clostridium difficile is an important pathogen in nosocomial infections. Although C. difficile toxins are considered to be major virulence factors, pathogenesis of C. difficile associated diseases remains to be determined. In this study, we investigated whether C. difficile flagellin is involved in the pathogenesi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.11.017
更新日期:2013-02-27 00:00:00
abstract::Dietary guidelines published in the past two decades have acknowledged the beneficial effects of myricetin, an important and common type of herbal flavonoid, against several human diseases such as inflammation, cardiovascular pathologies, and cancer. An increasing number of studies have shown the beneficial effects of...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2015.10.004
更新日期:2015-12-01 00:00:00
abstract::Years of researches have demonstrated that the imbalance of Th17 and Tregs contribute to the thyroid autoimmunity and the severity of autoimmune thyroid disease (AITD). The underlying mechanism comprises inherent genetic predisposition, abnormality of Th17 and Treg related biological molecules, and gut microbiota diso...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2017.11.026
更新日期:2018-01-01 00:00:00
abstract::The progeny of rats born and breastfed by mothers receiving dexamethasone (DEX) during pregnancy exhibits permanent reduction in body weight and adiposity but the precise mechanisms related to this programming are not fully understood. In order to clarify this issue, the present study investigated key aspects of lipop...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118765
更新日期:2021-01-15 00:00:00
abstract::To characterize the pharmacokinetics of protein-free granisetron in blood and brain we implanted microdialysis probes into the jugular vein and cerebral frontal cortex of the rat. Granisetron (3 or 6 mg/kg, i.v., n=6) was then administered, and microdialysates from blood and brain were collected from both sites and as...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00143-5
更新日期:1999-01-01 00:00:00
abstract::Malic enzyme activity in the soluble fraction of the neonatal brain of hypothyroid rats was observed to be lowered as compared to that of the control animals (p less than 0.01). Administration of triiodothyronine to the neonates of control animals resulted in significant enhancement (p less than 0.001) in the activity...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90428-0
更新日期:1987-12-28 00:00:00
abstract:AIMS:The present work evaluated the anti-inflammatory/antioxidant activity of a well characterized extract from Citrus bergamia Risso and Poiteau (CBE), containing neoeriocitrin, naringin, neohesperidin and other flavonoids, on human NCTC 2544 keratinocytes treated with interferon-gamma (IFN-γ) and histamine (H). MAIN...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.04.043
更新日期:2012-06-27 00:00:00
abstract::Although several reports imply that anticoagulants and preservatives contain zinc, the quantity of zinc in heparin, if any, has not been documented. Zinc concentration was determined by flame atomic absorption spectroscopy in varying dilutions of multiple commercially obtained samples of purified sodium heparin N = 15...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90348-x
更新日期:1983-12-26 00:00:00
abstract::The initial stage of atherosclerotic plaque formation involves oxidation of the phosphatidyl-choline moiety of low density lipoprotein (LDL) and subsequent uptake by macrophages. Ongoing uptake in developing plaque also may involve oxidized LDL and would require an oxidizing environment in plaque lipids. Atherosclerot...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02351-8
更新日期:1996-01-01 00:00:00
abstract::Obesity leads to various changes in the body. Among them, the existing inflammatory process may lead to an increase in the production of reactive oxygen species (ROS) and cause oxidative stress. Oxidative stress, in turn, can trigger mitochondrial changes, which is called mitochondrial dysfunction. Moreover, excess nu...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2017.11.019
更新日期:2018-01-01 00:00:00
abstract::A phencyclidine (PCP) receptor binding site has been solubilized in an active ligand-binding state from rat cerebral cortical membranes with sodium deoxycholate. Optimal receptor solubilization occurs at a detergent/protein ratio of 0.5 (w/w); for 5 mg protein/ml solubilized with 0.25% sodium deoxycholate, about 60% o...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90567-5
更新日期:1989-01-01 00:00:00