Abstract：
:Substrate stiffness is essential for cell functions, but the mechanisms by which cell sense mechanical cues are still unclear. Here we show that the frequency and the amplitude of spontaneous Ca2+ oscillations were greater in chick cardiomyocytes cultured on the stiff substrates than that on the soft substrates. The spontaneous Ca2+ oscillations were increased on stiff substrates. However, an eliminated dependence of the Ca2+ oscillations on substrate stiffness was observed after applying blocker of the large-conductance Ca2+-activated K+ (BK) channels. In addition, the activity of BK channels in cardiomyocytes cultured on the stiff substrates was decreased. These results provide compelling evidences to show that BK channels are crucial in substrate stiffness-dependent regulation of the Ca2+ oscillation in cardiomyocytes.
journal_name
Life Scijournal_title
Life sciencesauthors
Liu S,Gao X,Wu X,Yu Y,Yu Z,Zhao S,Zhao Hdoi
10.1016/j.lfs.2019.116802subject
Has Abstractpub_date
2019-10-15 00:00:00pages
116802eissn
0024-3205issn
1879-0631pii
S0024-3205(19)30729-5journal_volume
235pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:To examine the physiological significance of plasma ghrelin in generating pulsatile growth hormone (GH) secretion in rats, plasma GH and ghrelin levels were determined in freely moving male rats. Plasma GH was pulsatilely secreted as reported previously. Plasma ghrelin levels were measured by both N-RIA recognizing th...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00147-4
更新日期：2003-04-18 00:00:00
abstract：:Dose-effect curves were obtained for five specific components of stereotyped behavior (locomotor hyperactivity, rearing, sniffing, grooming and oral activity) elicited by the nonselective dopamine agonists N-propylnorapomorphine (NPA; 0.001-4.5 mg/kg) and apomorphine (APO; 0.3-10.0 mg/kg), the selective D2 agonists LY...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90324-4
更新日期：1988-01-01 00:00:00
abstract：:Nitric oxide (NO) may be an intercellular modulator within the central nervous system. L-arginine, which results in NO synthesis, increased food intake in mice while the inhibitor of NO synthesis, L-NG-nitro arginine (L-NO Arg) inhibited food intake in food deprived mice. L-arginine, but not D-arginine, partially reve...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90102-h
更新日期：1991-01-01 00:00:00
abstract：:Thymol induced superoxide production (O2-) by blood leukocytes was examined in various primates including man. Leukocytes of chimpanzee and hamadryas baboon cells showed only 35% of the maximal O2- production rate obtained in human cells, and those of the Japanese monkey and orang-utan failed to respond. In contrast, ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90735-1
更新日期：1987-09-28 00:00:00
abstract：:We examined the direct effect of magnesium ion on aldosterone production by adrenal cells using collagenase-dispersed zona-glomerulosa cells in rats. The effects of magnesium on aldosterone production stimulated by angiotensin II or ACTH were also investigated. Both magnesium sulphate (MgSO4) and magnesium chloride (M...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90327-5
更新日期：1989-01-01 00:00:00
abstract：:The present study was performed to determine if the attenuated pressor response to vasopressin in conscious non-estrous female rats is due in part to an enhanced V2-like receptor vasodilator action. In male rats, infusion of vasopressin at a rate of 1 ng.min(-1).kg body weight-1 (wt) resulted in an increase in mean ar...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00351-7
更新日期：1996-01-01 00:00:00
abstract：:The aggregation of melanin-granules within fish pigment cells (melanophores) can be elicited either by electrical stimulation of intrinsic nerves or by the addition of adrenergic agonists. The pigment aggregation seems to be mediated by alpha-2-adrenoceptors. In this investigation we have used various agonists and ant...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90595-8
更新日期：1985-09-16 00:00:00
abstract：:Epinephrine (E) infusions raise blood pressure and there is an excess incidence of hypertension among males and blacks. However, reports of E levels by ethnicity, gender, and blood pressure status are inconsistent. Insensitive assays, variability in plasma E levels within individuals, and the small size of most studie...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00568-3
更新日期：2000-05-05 00:00:00
abstract：:Diesel particles form a large component of the fine particle fraction (PM10) in urban air in the UK. During pollution episodes small increases in PM10 have been linked to detrimental health effects. The comparative toxicological effects of diesel exhaust and other well-characterised particles (carbon black, amorphous ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00141-6
更新日期：1998-01-01 00:00:00
abstract：:The role of the autonomic nervous system in the modulation of the immune response to emotional stimuli, was established in rats subjected to the passive avoidance test. An increase in splenic primary antibody response directed against SRBC was found after exposure of rats to the passive avoidance apparatus (novelty). ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90085-6
更新日期：1990-01-01 00:00:00
abstract：:We determined whether two naturally occurring steroids, cortisol and 17beta-estradiol (E2), can rapidly modulate the activity of an important membrane protein, human erythrocyte (RBC) Na+,K+-ATPase, an enzyme that does not bind either hormone directly. We also determined the membrane binding locations for cortisol and...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00360-4
更新日期：1999-01-01 00:00:00
abstract：:According to the two-state model of G-protein-coupled receptor (GPCR) activation, GPCRs isomerize from an inactive (R) state to an active (R*) state. In the R* state, GPCRs activate G-proteins. Agonist-independent R/R* isomerization is referred to as constitutive activity and results in an increase in basal G-protein ...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/s0024-3205(03)00654-4
更新日期：2003-09-19 00:00:00
abstract：AIMS:There have been recent reports that reconsolidation-based interventions attenuate drug reward memories in rodents. The insular cortex (IC) is an essential part of neural circuits that underlie cue-drug memory reconsolidation. GABAergic interneurons in the IC are a potent control on network excitability and play an...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117655
更新日期：2020-08-01 00:00:00
abstract：:Physical exercise is known to increase oxygen consumption to compensate for enhanced ATP consumption and thus to induce oxidative stress in tissues. Our previous data indicate that training significantly increased the hydroxyl radical level in rat tissues after physical exercise, but reduced the basal level in aged ra...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00468-8
更新日期：1998-01-01 00:00:00
abstract：AIMS:β-Adrenoceptors (β-ADRs) mediating the relaxation of rat superior mesenteric arteries (SMAs) were pharmacologically identified, and the effects of chemical sympathetic denervation on β-ADR-mediated relaxation were examined. MAIN METHODS:The tension changes of endothelium-denuded SMAs were isometrically recorded a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.117155
更新日期：2020-01-15 00:00:00
abstract：:The relative affinities of nortriptyline (NT) and its 10-hydroxymetabolites to rat brain muscarinic acetylcholine receptors have been determined by competition with 3H-quinuclidinyl benzilate binding. It is shown that the major NT metabolite, E-10-OH-NT, has only 1/18 the affinity of NT for the muscarinic receptor. Si...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90395-3
更新日期：1984-09-24 00:00:00
abstract：:Karyopherin α2 (KPNA2, also known as importinα-1), a member of the nuclear transporter family, is involved in the nucleocytoplasmic transport pathway of a variety of tumor-associated proteins. Recent studies have found that KPNA2 is overexpressed in various cancers, which is associated with poor prognosis. In addition...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2019.117140
更新日期：2020-01-15 00:00:00
abstract：:There is no satisfactory assay procedure of PAF in human whole blood in terms of sensitivity, reproducibility and simplicity. This is due to coexisting lipids from plasma and cellular membranes which inhibit measurement of PAF in various assay procedures, including bioassay. In the present study, an attempt was made t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00701-2
更新日期：1994-01-01 00:00:00
abstract：:SR 142801 is the first potent and selective non-peptide antagonist of the tachykinin NK3 receptor. It inhibited [MePhe7]NKB binding to its receptor from various species, including humans. SR 142801 was a competitive antagonist of [MePhe7]NKB-mediated contractions of guinea-pig ileum and inhibited the acetylcholine rel...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00413-m
更新日期：1995-01-01 00:00:00
abstract：:Polyamines (spermidine, spermine and putrescine) inhibited the adenylate cyclase activity in a concentration dependent manner in human erythrocyte plasma membranes. Spermidine (Spd) exhibited more inhibitory effect than spermine (Spm) and putrescine (Put). On the contrary, the addition of amino acids (arginine, glutam...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90055-v
更新日期：1990-01-01 00:00:00
abstract：:Despite reductions in beta-adrenoreceptor (beta-AR)-mediated inotropic effects induced by sustained sympathetic activation in cardiac disease, whether these changes necessarily result in reductions in systolic function under resting conditions (baseline function) is not clear. Moreover, possible compensatory mechanism...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.04.047
更新日期：2005-12-12 00:00:00
abstract：:LH stimulation is mediated by its own receptor at the first step of the cascade, after which intracellular cAMP increases to stimulate the transcription of steroidogenic acute regulatory protein (StAR) in mouse MA-10 Leydig tumor cells. StAR mediates the rate-limiting step of steroidogenesis, which is the transfer of ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(03)00698-2
更新日期：2003-10-17 00:00:00
abstract：:Using a ligation method, rat rectal epithelium was exposed to 2% sodium salicylate, and light and electron microscopic methods were used to assay for: 1) permeability of the epithelium to a marker dye, trypan blue, and 2) damage expressed in terms of disruption of the epithelial surface. Rectal mucosa was exposed to s...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90656-2
更新日期：1983-09-12 00:00:00
abstract：:The participation of the medullary structures in the total respiratory effect of opiates was studied by restricting the access of the delta-agonist D-ala2-D-leu5-enkephalin and the mu-agonist D-ala2-Me-Phe4-Met (O) ol5-enkephalin, to the ventral medullary surface, and by comparing their responses with those induced af...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90115-1
更新日期：1982-11-15 00:00:00
abstract：:The human placental villus tissue contains opioid receptors and peptides. The opioid peptides extracted from the villus tissue were fractionated using reverse-phase high performance liquid chromatography and a radio-receptor assay. The presence of dynorphin 1-8 was corroborated by mass spectrometric production of (M +...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90099-3
更新日期：1987-05-25 00:00:00
abstract：:Histogranin is a naturally-occurring pentadecapeptide with a structure 80% homologous with that a fragment-(86-100) of histone H4. First isolated from bovine adrenal medulla, the peptide was also shown to be present in the pituitary, brain, adrenal glands, blood plasma, lungs and spleen. At the subcellular level, hist...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(95)00068-2
更新日期：1995-03-03 00:00:00
abstract：:Lewis rats were trained to self-stimulate the medial forebrain bundle (MFB) using a rate-frequency paradigm. They were then tested for the effects of the cannabinoid receptor agonist CP 55,940, the selective cannabinoid receptor antagonist SR 141716 and the dopamine D1 receptor antagonist SCH 23390. CP 55,940 (0, 10, ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)01366-2
更新日期：2001-11-21 00:00:00
abstract：:The mechanism of the generation and the quantitative analysis of singlet oxygen (1O2) formed by the exposure of a hematoporphyrin derivative (HpD) to light was re-evaluated by electron spin resonance (ESR) combined with 2,2,6,6,-tetramethyl-4-piperidine (TMPD). The change from TMPD to 2,2,6,6,-tetramethyl-4-piperidine...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00835-7
更新日期：1997-01-01 00:00:00
abstract：:In order to determine the effect of relaxin and estradiol on collagen and noncollagen synthesis by mammary gland fibroblasts, fibroblasts isolated from guinea pig mammary glands were grown on plastic or Cytodex-3 collagen coated microcarriers. On plastic, estradiol (600 pg/ml) increased the incorporation of tritiated ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90460-0
更新日期：1984-11-26 00:00:00
abstract：:Immunoreactive- (IR-) somatostatin (SRIF), neuropeptide Y (NPY) and corticotropin-releasing factor (CRF) contents were investigated in the brain of tremor rats with absence-like seizure and spontaneously epileptic rats (SER), which is a genetically defined double-mutant (zi/zi, tm/tm) obtained by mating zitter homozyg...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)00302-m
更新日期：1995-01-01 00:00:00
