Abstract:
:High mobility group box 1 (HMGB1) is an abundant non-histone nuclear protein that functions as a structural protein of chromatin, regulating genome replication and recombination, mRNA transcription and DNA repair. HMGB1 has been implicated in the tumorigenesis of various cancer types, and the upregulation of HMGB1 has been demonstrated in glioma cells. However, the association between HMGB1 and the mitotic chromosomes in glioma remains uncharacterized. In the present study, the sub-cellular localization of HMGB1 in glioma tissues and cells was investigated. In addition, enhanced green fluorescent protein (EGFP)-tagging of the human HMGB1 protein and chromosome spreading were used to investigate the combination of HMGB1 with mitotic chromosomes. The results of the current study indicated that HMGB1 was localized to the nucleus and the cytoplasm, and it was determined to combine with the condensed chromosomes of proliferating cells in paraformaldehyde (PFA)-fixed glioma tissues. However, HMGB1 was also associated with interphase (but not mitotic chromosomes) when fixed with chilled methanol and 5% (v/v) acetic acid or PFA in vitro. Data from live cell imaging and chromosome spreading indicated the association of HMGB1 with mitotic chromosomes in glioma cells. The present results suggest that HMGB1 combines with mitotic chromosomes in glioma cells, and that the use of fixatives may result in the dissociation of the HMGB1-DNA interaction. Therefore, in live specimens and chromosome spreads, EGFP fusion proteins may represent an accurate indicator for the determination of the correct localization and interaction of HMGB1 in glioma cells.
journal_name
Oncol Lettjournal_title
Oncology lettersauthors
Jia L,Song H,Fan W,Song Y,Wang G,Li X,He Y,Yao Adoi
10.3892/ol.2019.11170subject
Has Abstractpub_date
2020-01-01 00:00:00pages
745-752issue
1eissn
1792-1074issn
1792-1082pii
OL-0-0-11170journal_volume
19pub_type
杂志文章相关文献
Oncology Letters文献大全abstract::Based on our previous analysis of neutral glycolipids in the human endometrium, the present authors already reported that the concentrations of glucosylceramide, lactosylceramide and globotriaosylceramide (Gb3Cer), in which both fatty acids and sphingosines in the ceramides are hydroxylated, exhibit a marked increase ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.5410
更新日期:2017-01-01 00:00:00
abstract::The detection of epidermal growth factor receptor (EGFR) mutations is necessary for the selection of suitable patients with non-small cell lung cancer (NSCLC) for treatment with EGFR tyrosine kinase inhibitors. Cytology specimens are known to be suitable for EGFR mutation detection, although tissue specimens should be...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7464
更新日期:2018-02-01 00:00:00
abstract::The aim of the present study was to investigate the optimal strategy and dosimetric measurement of thoracic radiotherapy based on three-dimensional (3D) modeling of mediastinal lymph nodes (MLNs). A 3D model of MLNs was constructed from a Chinese Visible Human female dataset. Image registration and fusion between reco...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8084
更新日期:2018-04-01 00:00:00
abstract::Emerging evidence has revealed that mitochondrial DNA (mtDNA) is encapsulated in plasma extracellular vesicles (EVs). However, the characteristics of mtDNA in EVs from patients with cancer remain largely unexplored, which greatly limits its clinical application. Whole genome and capture-based sequencing found that EV ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.11831
更新日期:2020-09-01 00:00:00
abstract::Cancer is a histologically and genetically heterogeneous population of tumor cells that exhibits distinct molecular profiles determined by epigenetic alterations. P-element-induced wimpy testis (PIWI) proteins in complex with PIWI-interacting RNA (piRNA) have been previously demonstrated to be involved in epigenetic r...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.5932
更新日期:2017-05-01 00:00:00
abstract::Solitary fibrous tumors (SFTs) represent a rare type of soft tissue tumor. Extrathoracic SFTs (ESFTs) in the soft tissues of the abdominopelvic cavity are extremely rare. Between January 2002 and January 2013, 10 patients were identified with abdominopelvic SFTs at the Northern Jiangsu People's Hospital. The clinicopa...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.1872
更新日期:2014-04-01 00:00:00
abstract::Peritoneal metastasis is an important prognostic factor for pancreatic cancer. The present study evaluated the possibility of diagnosing peritoneal metastasis by a photodynamic diagnosis using 5-aminolevulinic acid (5-ALA-PDD). In vitro, protoporphyrin IX (PpIX) accumulation was examined in the AsPC-1-GFP cell line fo...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8732
更新日期:2018-07-01 00:00:00
abstract::Liver kinase B1 (LKB1) encodes a serine/threonine kinase and functions as a tumor suppressor. LKB1 loss-of-function somatic mutations are frequently observed in sporadic types of cancer, particularly in lung cancer. Ectopic LKB1 induces growth arrest by upregulating p21/cyclin dependent kinase inhibitor 1A (WAF1) in L...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8741
更新日期:2018-07-01 00:00:00
abstract::Malignant mesothelioma in the mediastinum is rare and the majority of known cases have been reported as 'localized mesothelioma'. The present study reports a case of an upper mediastinal tumor, which was diagnosed through thoracoscopic surgery and surgical biopsies of the mass. A computed tomography scan revealed a gi...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1333
更新日期:2013-07-01 00:00:00
abstract::Local adjuvant treatment of giant cell tumours (GCTs) of the bone with phenol has led to a significant reduction in recurrence rates. In the current study, the optimal phenol concentration and duration of intralesional exposure were evaluated. Specimens of GCTs were exposed to various concentrations of phenol solution...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1244
更新日期:2013-05-01 00:00:00
abstract::Ovarian cancer (OVAC) remains the most lethal gynecological malignancy; it is ranked fifth among the most common types of cancer that affect women worldwide. Several aspects of the disease, including molecular pathogenesis, epidemiology, histological subtypes, poor prognosis at early stages due to the absence of speci...
journal_title:Oncology letters
pub_type: 杂志文章,评审
doi:10.3892/ol.2019.10587
更新日期:2019-09-01 00:00:00
abstract::MicroRNAs are proposed to serve vital functions in the regulation of tumor progression and invasion. However, the expression levels of miR-203 in non-small cell lung cancer (NSCLC) and its clinical significance remain unknown. In the present study, the association between B-cell-specific moloney murine leukemia virus ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3080
更新日期:2015-06-01 00:00:00
abstract::[This corrects the article DOI: 10.3892/ol.2017.6254.]. ...
journal_title:Oncology letters
pub_type: 杂志文章,已发布勘误
doi:10.3892/ol.2017.6794
更新日期:2017-10-01 00:00:00
abstract::The expression levels of cyclooxygenase (COX)-2 and the prostaglandin E2 (PGE2) content have been associated with poor prognosis in patients with colorectal cancer (CRC). There is a strong correlation between COX-2 expression and PGE2 production in tissues from CRC patients, suggesting an important role for COX-2 on t...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.5571
更新日期:2017-03-01 00:00:00
abstract::The novel synthesis of dibenzofluorene has been achieved by a cyclodehydration method. Several derivatives have also been prepared using this hydrocarbon. These compounds have been tested against a number of cancer cell lines in vitro, and useful selectivity has been observed. ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol_00000055
更新日期:2010-03-01 00:00:00
abstract::Pneumocystis jiroveci pneumonia (PCP) has long been recognized as a cause of mortality in immuno-compromised populations, including those with advanced lung cancer. Although Pneumocystis colonization has only recently been described due to the development of more sensitive molecular techniques, including polymerase ch...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.1052
更新日期:2013-02-01 00:00:00
abstract::Telomeres are located at the ends of chromosomes and protect them from degradation. Suppressing the activity of telomerase, a telomere-synthesizing enzyme, and maintaining short telomeres is a protective mechanism against cancer in humans. In most human somatic cells, the expression of telomerase reverse transcriptase...
journal_title:Oncology letters
pub_type: 杂志文章,评审
doi:10.3892/ol.2020.11659
更新日期:2020-08-01 00:00:00
abstract::The present study reports the clinical data of two patients with renal pelvis carcinoma and one patient with renal carcinoma who developed port-site metastasis following retroperitoneal laparoscopic surgery. The current study aimed to identify the cause and prognosis of the occurrence of port-site metastasis subsequen...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4541
更新日期:2016-06-01 00:00:00
abstract::The Na+/K+-ATPase α subunit is highly expressed in malignant cells. Ouabain, a cardioactive glycoside, binds to the Na+/K+-ATPase α subunit and inhibits the activity of Na+/K+-ATPase. In the present study, the effect of ouabain on the migration of A549 cells was analyzed using the wound healing and transwell chamber m...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1406
更新日期:2013-08-01 00:00:00
abstract::Overexpression of adenosine triphosphate-binding cassette (ABC) transport protein is emerging as a critical contributor to anticancer drug resistance. The eukaryotic translation initiation factor (eIF) 4F complex, the key modulator of mRNA translation, is regulated by the phosphoinositide 3-kinase-AKT-mammalian target...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6049
更新日期:2017-06-01 00:00:00
abstract::Inhibition of nuclear factor-κB (NF-κB) results in antitumor activity in leukemia cells, and may be a potential therapeutic strategy for the treatment of leukemia. However, a significant limitation of NF-κB inhibition in the treatment of leukemia is the low efficiency of this technique. NF-κB inhibitor treatment induc...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3786
更新日期:2015-12-01 00:00:00
abstract::We compared the clinical effects and prognosis of patients receiving lymph node dissection after surgical removal of the thyroid tissues and those not receiving it after the removal. A total of 80 patients diagnosed with differentiated thyroid carcinoma (DTC) by our hospital from March 2012 to March 2014 were successi...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.5450
更新日期:2017-01-01 00:00:00
abstract::Previous studies have shown that the activation of the β2 adrenergic receptor (ADRB2) can stimulate several signaling pathways that promote tumor growth and metastasis. β-adrenergic antagonism may have a beneficial role in cancer treatment; however, little is known about the effect of ADRB2 inhibition on the growth of...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8847
更新日期:2018-08-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is generally considered one of the most common gastrointestinal malignant tumors, characterized by high invasiveness and metastatic rate, as well as insidious onset. A relationship between carcinogenicity and aberrant microRNA-139-5p (miR-139-5p) expression has been identified in multipl...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.11031
更新日期:2019-12-01 00:00:00
abstract::Melanotic neuroectodermal tumor of infancy (MNTI) is a rare, fast-growing, benign tumor originating from the neural crest. The tumor most often occurs during the first year of life. The predilection site of MNTI is the anterior maxilla, whereas lesions of the mandible are uncommon and account for only 6% of all cases....
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2665
更新日期:2015-01-01 00:00:00
abstract::The present study investigated the correlations of the Tspan-1 gene expression with the clinical characteristics and survival prognoses of patients with advanced gastric cancer. A total of 150 patients with advanced gastric cancer were enrolled in the present study, of whom 84 were at stage II and 66 were at stage III...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6529
更新日期:2017-09-01 00:00:00
abstract::The present study aimed to measure the expression of WNT1 in ameloblastoma (AB). Immunohistochemistry was used to observe changes in WNT1 expression in 80 AB samples, 10 keratocystic odontogenic tumor (KCOT) samples and 10 normal oral mucosa (NOM) samples. Western blotting and reverse transcription-quantitative polyme...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8820
更新日期:2018-08-01 00:00:00
abstract::Fluoro-edenite is a natural mineral species initially isolated in Biancavilla, Sicily. The fibres are similar in size and morphology to certain amphibolic asbestos fibres, the inhalation of which may cause chronic inflammation and cancer. Occupational asbestos exposure is known to be associated with pleural and lung d...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.2978
更新日期:2015-05-01 00:00:00
abstract::The present study aimed to investigate potential recurrence-risk biomarkers based on significant pathways for Luminal A breast cancer through gene expression profile analysis. Initially, the gene expression profiles of Luminal A breast cancer patients were downloaded from The Cancer Genome Atlas database. The differen...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.7940
更新日期:2018-04-01 00:00:00
abstract::This study investigated energy metabolism and its association with inflammatory cytokines and appetite- regulating hormones in patients with gastrointestinal cancer. Subjects were inpatients scheduled to undergo therapeutic intervention for diagnosed gastrointestinal cancer. Nutritional status on admission was assesse...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.11662
更新日期:2020-08-01 00:00:00