Chemo-enzymatic synthesis of imidazolium-tagged sialyllactosamine probes.

Abstract:

:Two novel α-linked sialyltrisaccharide imidazolium-type probes (ITags) based on the structures of biologically relevant 6'-sialyllactosamine and 3'-sialyllactosamine were efficiently and stereoselectively prepared using a chemo-enzymatic approach. The apparent kinetic parameters for the enzyme catalyzed transformations with α-2,3-sialyltransferase (α-2,3-ST) and α-2,6-sialyltransferase (α-2,6-ST) were measured by LC-MS using the ionic probes. This strategy demonstrates the suitability of the ITags to probe glycosyltransferase activity and their versatility in the preparation of sialylated epitopes for glycobiology research.

journal_name

Bioorg Med Chem Lett

authors

Sittel I,Galan MC

doi

10.1016/j.bmcl.2015.07.049

subject

Has Abstract

pub_date

2015-10-01 00:00:00

pages

4329-32

issue

19

eissn

0960-894X

issn

1464-3405

pii

S0960-894X(15)00753-2

journal_volume

25

pub_type

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