Abstract:
:The single-stranded RNA (ssRNA) bacteriophages are among the simplest known viruses with small genomes and exceptionally high mutation rates. The number of ssRNA phage isolates has remained very low, but recent metagenomic studies have uncovered an immense variety of distinct uncultured ssRNA phages. The coat proteins (CPs) in these genomes are particularly diverse, with notable variation in length and often no recognizable similarity to previously known viruses. We recombinantly expressed metagenome-derived ssRNA phage CPs to produce virus-like particles and determined the three-dimensional structure of 22 previously uncharacterized ssRNA phage capsids covering nine distinct CP types. The structures revealed substantial deviations from the previously known ssRNA phage CP fold, uncovered an unusual prolate particle shape, and revealed a previously unseen dsRNA binding mode. These data expand our knowledge of the evolution of viral structural proteins and are of relevance for applications such as ssRNA phage-based vaccine design.
journal_name
Sci Advjournal_title
Science advancesauthors
Rūmnieks J,Liekniņa I,Kalniņš G,Šišovs M,Akopjana I,Bogans J,Tārs Kdoi
10.1126/sciadv.abc0023subject
Has Abstractpub_date
2020-09-02 00:00:00issue
36issn
2375-2548pii
6/36/eabc0023journal_volume
6pub_type
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