Abstract:
:Osteoclasts are important target cells for osteoporosis treatment. Recently, a real-time cell analysis (RTCA) system was developed to observe cell morphology and adhesion; however, the use of RTCA to study osteoclastogenesis has not been reported. Here, we investigated whether osteoclast formation could be monitored in real-time using RTCA. The cell index determined via electrical impedance using RTCA, and the number of osteoclasts exhibited a significant positive correlation. RTCA was useful for determining the effect of (-)-epigallocatechin-3-gallate on the inhibition of bone resorption. We established a new method of measuring osteoclast formation in real-time using RTCA.
journal_name
J Pharmacol Scijournal_title
Journal of pharmacological sciencesauthors
Emori H,Iwai S,Ryu K,Amano H,Sambe T,Kobayashi T,Oguchi T,Ohura K,Oguchi Kdoi
10.1016/j.jphs.2015.05.002subject
Has Abstractpub_date
2015-06-01 00:00:00pages
87-91issue
2eissn
1347-8613issn
1347-8648pii
S1347-8613(15)00090-0journal_volume
128pub_type
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