当前位置： SCI文献检索 > BRAIN RESEARCH期刊下所有文献 > p53 inhibition provides a pivotal protective effect against ischemia-reperfusion injury in vitro via mTOR signaling.

p53 inhibition provides a pivotal protective effect against ischemia-reperfusion injury in vitro via mTOR signaling.

Abstract：

:Tumor suppressor p53 has recently been reported to have numerous functions independent of tumorigenesis, including neuronal survival during ischemia. The mammalian target of rapamycin (mTOR) signaling pathway plays a central role in the regulation of metabolism, cell growth, development, and cell survival. Our recent work has demonstrated the neuroprotective effects of the mTOR pathway. Considering that p53 is also an important regulator of mTOR, to further clarify the role of p53 and the mTOR signaling pathway in neuronal ischemic-reperfusion injury, we used mouse primary mixed cultured neurons with an oxygen glucose deprivation (OGD) model to mimic an ischemic-reperfusion injury in vitro. A lentiviral system was also used to inhibit or overexpress p53 to determine whether p53 alteration affects OGD and reperfusion injury. Our results show that activated p53 was induced and it suppressed mTOR expression in primary mixed cultured neurons after OGD and reperfusion. Inhibiting p53, using either a chemical inhibitor or lentiviral-mediated shRNA, exhibited neuroprotective effects in primary cultured neurons against OGD and reperfusion injury through the upregulation of mTOR activity. Such protective effects could be reversed by rapamycin, an mTOR inhibitor. Conversely, p53 overexpression tended to exacerbate the detrimental effects of OGD injury by downregulating mTOR activity. These results suggest that p53 inhibition has a pivotal protective effect against an in vitro ischemia-reperfusion injury via mTOR signaling and provides a potential and promising therapeutic target for stroke treatment.

journal_name

Brain Res

journal_title

Brain research

authors

Li X,Gu S,Ling Y,Shen C,Cao X,Xie R

doi

10.1016/j.brainres.2015.02.009

subject

Has Abstract

pub_date

2015-04-24 00:00:00

pages

31-8

eissn

0006-8993

issn

1872-6240

pii

S0006-8993(15)00093-1

journal_volume

1605

pub_type

杂志文章

相关文献

BRAIN RESEARCH文献大全
  • Re-epithelialization of the rabbit cornea is regulated by opioid growth factor.

    abstract：:An endogenous opioid peptide, [Met5]-enkephalin, termed opioid growth factor (OGF) is a tonically active, autocrine-produced inhibitory molecule related to developing, neoplastic, renewing and healing tissues. The present investigation was designed to examine the role of OGF on corneal epithelial wound closure in the ...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/s0006-8993(98)00610-6

    authors： Zagon IS,Sassani JW,McLaughlin PJ

    更新日期：1998-08-24 00:00:00

  • Dorsomedial hindbrain participation in glucoprivic feeding response to 2DG but not 2DG-induced hyperglycemia or activation of the HPA axis.

    abstract：:2-Deoxy-d-glucose (2DG) is a glucose analogue that inhibits intracellular utilization of glucose and produces a characteristic behavioral response known as glucoprivic feeding. The area postrema (AP) is a caudal hindbrain structure shown previously to be involved in 2DG-induced glucoprivic feeding. In addition, periph...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/s0006-8993(98)00528-9

    authors： Edmonds BK,Edwards GL

    更新日期：1998-08-10 00:00:00

  • Changes in presynaptic protein NACP/alpha-synuclein in an ischemic gerbil hippocampus.

    abstract：:We observed temporal changes in NACP (precursor protein of non-Abeta component of Alzheimer's disease amyloid), a presynaptic protein a.k. a. alpha-synuclein, in the hippocampus after 5 min ischemia. Intense NACP immunoreactivity was seen transiently around cerebral blood vessels in the CA1 subfield on day 4, and NACP...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/s0006-8993(98)00033-x

    authors： Ishimaru H,Uéda K,Takahashi A,Maruyama Y

    更新日期：1998-03-30 00:00:00

  • Neuronal mechanism underlying dystonia induced by bicuculline injection into the putamen of the cat.

    abstract：:Microinjection of bicuculline (Bic) into the unilateral putamen (Put) of the cat resulted in periodic occurrence of contralateral movement of the head (dystonia). Corresponding to this dystonic movement, repetitive spikes of the field potential occurring in the Put of the injected side and strong inhibition of the ext...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(95)00190-2

    authors： Yamada H,Fujimoto K,Yoshida M

    更新日期：1995-04-24 00:00:00

  • Discordant changes between immunoreactive ACTH and beta-endorphin in rat brain and pituitary during early development.

    abstract：UNLABELLED:Discordant changes in brain concentrations of immunoreactive (IR-) adrenocorticotropic hormone (ACTH) or beta-endorphin, peptides derived from pro-opiomelanocortin (POMC), have been reported during the latter part of development and with subsequent aging. These changes were believed to be due to age-related ...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(86)90847-4

    authors： Kapcala LP

    更新日期：1986-02-05 00:00:00

  • Inhibition of dehydration induced drinking in rats by reduction of CSF Na concentration.

    abstract：:Male rats were dehydrated for 22 h and then given 4 h intracerebroventricular (i.c.v.) infusions which commenced 2 h prior to the beginning of a 2-h fluid access period. I.c.v. infusion of iso-osmotic 0.27 M mannitol-CSF more than halved the amount of water normally drunk by dehydrated rats during the fluid access per...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(87)91437-5

    authors： Osborne PG,Denton DA,Weisinger RS

    更新日期：1987-05-26 00:00:00

  • A photothrombotic ring stroke model in rats with or without late spontaneous reperfusion in the region at risk.

    abstract：:This study aimed at developing a dual setup of the photothrombotic ring stroke model with or without late spontaneous reperfusion in the region at risk and to explore the morphological consequences. The exposed crania of adult male Wistar rats were subjected to a ring-shaped laser-irradiation beam (o.d. 5.0 mm, 0.35 m...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/s0006-8993(99)02152-6

    authors： Hu X,Brännström T,Gu W,Wester P

    更新日期：1999-12-04 00:00:00

  • Auditory repetition enhancement at short interstimulus intervals for frequency-modulated tones.

    abstract：:Frequency-modulated (FM) sweeps are important components of most natural sounds. To examine the processing of these stimuli we applied a two-tone paradigm. Repeated stimulus presentation usually leads to reduced neuronal responses. However, in a former study repetition enhancements which have been observed when FM ton...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/j.brainres.2011.07.009

    authors： Heinemann LV,Kaiser J,Altmann CF

    更新日期：2011-09-09 00:00:00

  • Effects of sensory deafferentation on glucose metabolism of muscles during locomotion.

    abstract：:We measured the uptake of a radiolabeled analogue of glucose ([3H]FDG) into muscles during treadmill walking so as to determine whether sensory deafferentation of a limb affects the muscles' metabolic response to the exercise. The muscles on the deafferented side took up less tracer than those in the intact side with ...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(85)91526-4

    authors： Eidelberg E,Miller AL

    更新日期：1985-02-18 00:00:00

  • Intracerebroventricular 2-DG causes feeding in the absence of other signs of glucoprivation.

    abstract：:Intracerebroventricular (ICV) infusions of 2-deoxy-D-glucose (2-DG) elicited hyperglycemia and increased feeding at short latencies. If access to food was prevented for 6 h post-2-DG infusion, when blood glucose levels were normal and brain 2-DG concentrations were virtually zero, the rats still ate significantly more...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：

    authors： Engeset RM,Ritter RC

    更新日期：1980-11-24 00:00:00

  • Water maze training in aged rats: effects on brain metabolic capacity and behavior.

    abstract：:The effects of Morris water maze training on brain metabolism and behavior were compared between aged (20-22 months) and young (2-4 months) Fischer 344 male rats. Each group had yoked controls, which swam the same amount of time as the trained rats but without the platform. This was followed after 9 days by quantitati...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/s0006-8993(02)02545-3

    authors： Villarreal JS,Gonzalez-Lima F,Berndt J,Barea-Rodriguez EJ

    更新日期：2002-06-07 00:00:00

  • Thyrotropin-releasing hormone (TRH) blocks glucagon-induced hyperglycemia in mice: dissociation of the antihyperglycemic and pituitary actions of TRH.

    abstract：:Thyrotropin-releasing hormone (TRH) prevents the development of drug- or stress-induced hyperglycemia in mice through action at central sites. To study the role of the pituitary in mediating this action, we have evaluated the effect of TRH analogs lacking hypophysiotropic activity, acid-TRH and DN 1417, in blocking gl...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(88)90135-7

    authors： Amir S

    更新日期：1988-07-05 00:00:00

  • Removing zinc from synaptic vesicles does not impair spatial learning, memory, or sensorimotor functions in the mouse.

    abstract：:Zinc-enriched (ZEN) neurons are distributed widely throughout the brain and spinal cord. Synaptic vesicle zinc in these neurons is thought to function as a neuromodulator upon its release into the synaptic cleft. Consistent with this possibility, zinc or zinc chelators can alter spatial learning, working memory, and n...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/s0006-8993(00)03220-0

    authors： Cole TB,Martyanova A,Palmiter RD

    更新日期：2001-02-09 00:00:00

  • Vagotomy does not alter circannual body weight cycles in the hibernator Citellus lateralis.

    abstract：:To evaluate a hypothesized role of the vagus in the circannual metabolic cycle of hibernators, measures of annual weight gain, weight loss and cycle period were taken for the ground squirrel, Citellus lateralis, maintained in constant environmental conditions before and after subdiaphragmatic vagotomy. The surgery did...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(86)90996-0

    authors： Powley TL,Fox EA

    更新日期：1986-01-29 00:00:00

  • Neuromuscular patterns of stereotypic hindlimb behaviors in the first two postnatal months. I. Stepping in normal kittens.

    abstract：:Neuromuscular patterns associated with the development of hindlimb stepping behaviors were studied from birth to postnatal day 60 in normal kittens. Hindlimb muscles were chronically implanted with EMG electrodes at birth to characterize interlimb coordination and intralimb synergies during development of overground a...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0165-3806(88)90084-3

    authors： Bradley NS,Smith JL

    更新日期：1988-01-01 00:00:00

  • Effects of concurrent manipulations of cholinergic and noradrenergic function on learning and retention in mice.

    abstract：:Interactions between the neuromodulators acetylcholine and norepinephrine (NE) have been reported in both developmental neural plasticity and learning and memory. In a test of the generality of this phenomenon, we assessed the amnestic effects of the muscarinic antagonist scopolamine in normal and NE-depleted mice. Pr...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(89)91391-7

    authors： Decker MW,McGaugh JL

    更新日期：1989-01-16 00:00:00

  • Developmental ethanol exposure alters the morphology of mouse prefrontal neurons in a layer-specific manner.

    abstract：:Chronic developmental exposure to ethanol can lead to a wide variety of teratogenic effects, which in humans are known as fetal alcohol spectrum disorders (FASD). Individuals affected by FASD may exhibit persistent impairments to cognitive functions such as learning, memory, and attention, which are highly dependent o...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/j.brainres.2017.10.005

    authors： Louth EL,Luctkar HD,Heney KA,Bailey CDC

    更新日期：2018-01-01 00:00:00

  • Long-lasting effects of naltrexone, an opioid receptor antagonist, on cell proliferation in developing rat forebrain.

    abstract：:Several studies have demonstrated in the past that endogenous opioid peptides and opioid receptors may be involved as mediators of brain tissue growth and function in the neonate. Applying histological and autoradiographic methods, we have examined the effect of the mu-receptor-specific antagonist, naltrexone, on the ...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(89)90515-5

    authors： Schmahl W,Funk R,Miaskowski U,Plendl J

    更新日期：1989-05-08 00:00:00

  • The striatopallidal and striatonigral projections: two distinct fiber systems in primate.

    abstract：:The use of the fluorescence retrograde double-labeling method has revealed that striatal neurons projecting to the globus pallidus in the squirrel monkey are mainly confined to the putamen whereas those projecting to the substantia nigra occur mostly in the caudate nucleus. Only about 10% of the striatal neurons were ...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(84)91224-1

    authors： Parent A,Bouchard C,Smith Y

    更新日期：1984-06-15 00:00:00

  • Oxygen-glucose deprivation (OGD) and interleukin-1 (IL-1) differentially modulate cathepsin B/L mediated generation of neuroprotective perlecan LG3 by neurons.

    abstract：:Brain extracellular matrix (ECM) is highly degraded after cerebral ischemia. The perlecan c-terminal fragment LG3 is generated at increased levels by proteolytic processing as long as 3 days after ischemia. It has previously been shown that oxygen-glucose deprivation (OGD), reperfusion and interleukin-1 α (IL-1α) stim...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/j.brainres.2011.12.027

    authors： Saini MG,Bix GJ

    更新日期：2012-02-15 00:00:00

  • Telmisartan suppresses cerebral injury in a murine model of transient focal ischemia.

    abstract：:The beneficial effects of angiotensin II type 1 (AT1) receptor blockers (ARB) in cerebrovascular disease have been shown in clinical trials. However, the effects of ARBs vary based on their unique pharmacologic properties. In this study, we focused on telmisartan, a fat-soluble ARB with selective peroxisome proliferat...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/j.brainres.2010.03.101

    authors： Kasahara Y,Taguchi A,Uno H,Nakano A,Nakagomi T,Hirose H,Stern DM,Matsuyama T

    更新日期：2010-06-22 00:00:00

  • Loss of active avoidance of responding after lateral hypothalamic injections of 6-hydroxydopamine.

    abstract：:After injections of 6-hydroxydopamine (6-OHDA) along the medial forebrain bundle in the lateral hypothalamus, rats failed to acquire a one-way active avoidance response or failed to perform a previously acquired active avoidance response. Such rats, however, acquired a passive avoidance response and a conditioned tast...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(75)90659-9

    authors： Smith GP,Levin BE,Ervin GN

    更新日期：1975-05-09 00:00:00

  • Laminar origins of spinal projection neurons to the periaqueductal gray of the rat.

    abstract：:Attempts were made to determine the cells of origin of the spinal projections to the PAG using retrograde axoplasmic transport techniques of HRP and fluorescent markers. Following injections limited to the lateroventral subnucleus of the PAG, labels were transported to the contralateral laminae I, III, V, VII and X pr...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(83)91127-7

    authors： Liu RP

    更新日期：1983-03-28 00:00:00

  • Event-related potential evidence of accessing gender stereotypes to aid source monitoring.

    abstract：:Source memory for the speaker's voice (male or female) was investigated when semantic knowledge (gender stereotypes) could and could not inform the episodic source judgment while event-related potentials (ERPs) were recorded. Source accuracy was greater and response times were faster when stereotypes could predict the...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/j.brainres.2012.11.013

    authors： Leynes PA,Crawford JT,Radebaugh AM,Taranto E

    更新日期：2013-01-23 00:00:00

  • Failure of deferoxamine, an iron chelator, to improve outcome after collagenase-induced intracerebral hemorrhage in rats.

    abstract：:Intracerebral hemorrhage (ICH) is a devastating stroke with no clinically proven treatment. Deferoxamine (DFX), an iron chelator, is a promising therapy that lessens edema, mitigates peri-hematoma cell death, and improves behavioral recovery after whole-blood-induced ICH in rodents. In this model, blood is directly in...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/j.brainres.2009.10.058

    authors： Warkentin LM,Auriat AM,Wowk S,Colbourne F

    更新日期：2010-01-14 00:00:00

  • Differential distribution of cellular forms of beta-amyloid precursor protein in murine glial cell cultures.

    abstract：:The production and localization of cell-associated forms of beta-amyloid precursor protein (APP) of Alzheimer's disease was investigated in primary cultures of mouse glial cells. In both oligodendrocytes and astrocytes, immunofluorescence staining with an antibody against the carboxy terminus of APP revealed an intens...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(92)90898-j

    authors： Mizuguchi M,Ikeda K,Kim SU

    更新日期：1992-07-03 00:00:00

  • Locus ceruleus lesions block pulsatile LH release in ovariectomized rats.

    abstract：:Luteinizing hormone (LH) secretion during the reproductive cycle and in ovariectomized (OVX) rats is pulsatile and this pattern of secretion is determined by intermittent discharges of LH-releasing hormone (LHRH) into the hypophysial portal vessels. LHRH secretion is probably controlled by prior pulsatile norepinephri...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/s0006-8993(99)01548-6

    authors： Anselmo-Franci JA,Rocha-Barros VM,Franci CR,McCann SM

    更新日期：1999-06-26 00:00:00

  • Cellular substrates for interactions between dynorphin terminals and dopamine dendrites in rat ventral tegmental area and substantia nigra.

    abstract：:Dynorphin and other kappa opioid agonists are thought to elicit aversive actions and changes in motor activity through direct or indirect modulation of dopamine neurons in ventral tegmental area (VTA) and substantia nigra (SN), respectively. We comparatively examined the immunoperoxidase localization of anti-dynorphin...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(93)90693-h

    authors： Pickel VM,Chan J,Sesack SR

    更新日期：1993-02-05 00:00:00

  • Localization of GAD- and GABA-like immunoreactivity in ground squirrel retina: retrograde labeling demonstrates GAD-positive ganglion cells.

    abstract：:Glutamic acid decarboxylase (GAD)- and gamma-aminobutyric acid (GABA)-like immunoreactivity was examined in the retina of the 13-lined ground squirrel (Spermophilus tridecemlineatus). Labeling was observed in the inner nuclear layer (INL), inner plexiform layer (IPL) and ganglion cell layer (GCL). The immunoreactive c...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/0006-8993(91)91346-3

    authors： Lugo-García N,Blanco RE

    更新日期：1991-11-08 00:00:00

  • Generalization of seizures parallels the formation of "dark" neurons in the hippocampus and pontine reticular formation after focal-cortical application of 4-aminopyridine (4-AP) in the rat.

    abstract：:Distribution and time course of the occurrence of "dark" neurons were compared with the EEG activity and behavior of rats during 4-aminopyridine (4-AP) induced epileptic seizures. A crystal of the K(+) channel blocker 4-AP (0.5 mg/kg) was placed onto the exposed parieto-occipital cortex of Halothane-anesthetized rats ...

    journal_title：Brain research

    pub_type： 杂志文章

    doi：10.1016/j.brainres.2008.06.044

    authors： Baracskay P,Szepesi Z,Orbán G,Juhász G,Czurkó A

    更新日期：2008-09-04 00:00:00