Changes in the TNF-alpha/IL-10 ratio in hyperglycemia-associated pregnancies.

Abstract:

AIMS:TNF-α is a diabetogenic cytokine associated with adverse outcomes during pregnancy that can be counterbalanced by IL-10. We have investigated IL-10 and TNF-α balance at maternal and placental levels in hyperglycemia-associated pregnancies. METHODS:One hundred and ninety-two pregnant women participated, which included normoglycemic women (ND) and women with mild gestational hyperglycemia (MGH), gestational diabetes mellitus (GDM) and type 2 diabetes mellitus (DM2). Maternal plasma and placental tissue IL-10 and TNF-α levels were measured by ELISA and placental TNF-α was also immunolocalized. RESULTS:Maternal plasma TNF-α levels were highest in GDM (p=0.0190), whereas TNF-α levels were highest in placental tissues in DM2 (p=0.0095). Immunohistochemistry also showed strong reactivity with anti-TNF-α antibody in the villous structures in the DM2 group. Conversely, IL-10 levels were lowest in maternal plasma of the DM2 group (p=0.0228). The TNF-α/IL-10 ratio in maternal plasma progressively increased with the severity of hyperglycemia (p<0.0001), being highest in placenta of the DM2 group (p=0.0150). In both, plasma and placenta, TNF-α/IL-10 ratio were correlated with mean maternal glycemia and HbA1c levels. CONCLUSIONS:Alterations of placenta and serum TNF-α/IL-10 balance with predominance of TNF-α were correlated with the severity of hyperglycemia during gestation. This association may offer insight into the pathogenesis of gestational hyperglycemia and associated pregnancy outcomes.

authors

Moreli JB,Corrêa-Silva S,Damasceno DC,Sinzato YK,Lorenzon-Ojea AR,Borbely AU,Rudge MV,Bevilacqua E,Calderon IM

doi

10.1016/j.diabres.2015.01.005

subject

Has Abstract

pub_date

2015-03-01 00:00:00

pages

362-9

issue

3

eissn

0168-8227

issn

1872-8227

pii

S0168-8227(15)00013-3

journal_volume

107

pub_type

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