Abstract:
:Previously described methods of phenotyping red cells sensitised with IgG using the indirect antiglobulin test required the dissociation of the coating protein. Based on an entirely different principle, Fab fragments of anti-human IgG (Fab anti-IgG) were used to block the antiglobulin binding sites on cell-bound IgG molecules, removing the necessity to dissociate them from the red cell. Fab anti-IgG was found to be effective in blocking interfering IgG on in vivo and in vitro IgG-sensitised red cells, permitting successful red cell phenotyping. Strongly IgG-sensitised samples which could not be fully neutralised by chloroquine diphosphate (CDP) or blocked with Fab anti-IgG alone could usually be phenotyped using a combination of both these methods. This new procedure may be of use in immunohaematology laboratories.
journal_name
Vox Sangjournal_title
Vox sanguinisauthors
Henry SM,Baird SJ,Woodfield DGdoi
10.1111/j.1423-0410.1987.tb04939.xsubject
Has Abstractpub_date
1987-01-01 00:00:00pages
147-50issue
3eissn
0042-9007issn
1423-0410journal_volume
53pub_type
杂志文章相关文献
VOX SANGUINIS文献大全abstract::Radioimmunoassays were prepared using two anti-A and one anti-B reagents. The specificity of the procedures was assessed with 13 artificial antigens. The amounts of A and B natural antigens in saliva of ABH secretors of known Lewis phenotype were measured with these assays. The results confirmed that the average amoun...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::Following transfusion, red cells labeled with nonradioactive chromium (52Cr) can be detected in the circulation using atomic absorption spectrophotometry. Both in rhesus monkeys and in human subjects, the survival of red cells labeled with 52Cr was found to be insignificantly different from that of cells labeled with ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1990.tb02091.x
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Leucocyte-derived cytokines accumulate in stored whole blood. Pre-storage leucocyte depletion has reduced cytokine levels and, consequently, febrile non-haemolytic transfusion reactions. As leucocyte filtration and component separation can be performed until 24 h after donation, we hypothesize...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2008.01132.x
更新日期:2009-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The International Haemovigilance Network collects aggregate data on complications of blood donation from member haemovigilance systems (HVS). We analysed the data collected in 2006-2016 in order to learn from it and consider future improvements. MATERIALS AND METHODS:National HVS entered annu...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.13048
更新日期:2020-12-05 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Our post-thaw cell recovery rates differed substantially in interlaboratory comparisons of identical samples, potentially due to different temperatures during cell staining. MATERIALS AND METHODS:Viable CD34(+) cells and leucocyte (WBC) subtypes were quantified by multiparameter single-platfo...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12398
更新日期:2016-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Despite long being a mainstay in describing platelet activation via degranulation, interlaboratory variation remains an issue in measurement of membrane CD62P by flow cytometry. Our objective was to identify actions that may minimize this variation. MATERIALS AND METHODS:Sixteen laboratories ...
journal_title:Vox sanguinis
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1111/vox.12023
更新日期:2013-07-01 00:00:00
abstract::OCTAPLAS is a cell-free standardized blood group specific human coagulation active plasma for transfusion. The viral safety is mainly based on the treatment with solvent/detergent. SD is known to irreversibly inactivate the lipid enveloped viruses including HIV 1 + 2, HBV and HCV and has been recommended both by Europ...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:10.1111/j.1423-0410.1998.tb05474.x
更新日期:1998-01-01 00:00:00
abstract::Present platelet storage media often designated platelet additive solutions (PAS) basically contain acetate, citrate and phosphate and recently also potassium and magnesium. However, there seems to be an increasing interest in developing PASs that can be used also after further reduction of residual plasma content bel...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:10.1111/vox.12172
更新日期:2014-10-01 00:00:00
abstract::We simulated the use of massive volumes of crystalloid fluids as a treatment of acute plasma loss in a standardized experimental model and studied the factors determining the retention or excretion of the resulting acute hypoproteinemic fluid overload, its distribution within the body, and its treatment with concentra...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1977.tb04481.x
更新日期:1977-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Red-blood-cell (RBC) transfusion is associated with lung injury, which is further exacerbated by mechanical ventilation. Manufacturing methods of blood products differ globally and may play a role in the induction of pulmonary cell activation through alteration of the immunomodulatory property...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12911
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The risk of transfusion-transmitted human T-lymphotropic virus type 1 infection (TT-HTLV-1) after prestorage leucocyte reduction (LR) remains unknown, as the proviral load in the blood component that would cause TT-HTLV-1 is undetermined. On the basis of the distribution of HTLV-1 proviral loa...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.12263
更新日期:2015-08-01 00:00:00
abstract::Sera from 2,829 participants in a population health survey were tested for the presence of a heterophilic antibody, detectable by immunofluorescence. The antibody was found in 9.8% of the sera from women and in 12.4% of the sera from men. The age-specific prevalence of the antibody was relatively constant in women but...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1980.tb02973.x
更新日期:1980-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:A 2-year-old female with neuroblastoma needed In(b-), E- red blood cells (RBCs). No units were available at the blood centre (BC) nor in the rare donor programme member's inventories. BC's Immunohematology Reference Laboratory (IRL) and its marketing department concentrated on recruiting and t...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/vox.13053
更新日期:2020-12-21 00:00:00
abstract::To determine what effect different preparative protocols and different platelet storage bags have on in vitro release, platelet concentrates were prepared according to two different protocols (group I: 2,200 g for 3.5 min followed by 4,900 g for 5 min; group II: 1,000 g for 9 min and 3,000 g for 20 min). The storage b...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:To demonstrate the application of leukocyte removal filters made of a new type of filter material - superfine glass fiber - for depleting leukocytes in SAGM red cell suspensions and preventing nonhemolytic transfusion reactions. MATERIALS AND METHODS:The extent of leukocyte depletion and red ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1159/000031015
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:White blood cell (WBC) fragments in platelet concentrates (PCs) may induce allo-immunization in the recipient. MATERIALS AND METHODS:As the level of WBC fragments can differ between PCs produced using different methods, we compared PCs prepared by using the buffy-coat method (BC-PCs) in plasm...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2005.00634.x
更新日期:2005-05-01 00:00:00
abstract::The formation of mono(ethylhexyl)phthalate (MEHP) from di(ethylhexyl)phthalate in human plasma stored in bags of polyvinylchloride has been studied. Substantial amounts were formed and in ten bags from 4 to 56microgram/ml were found. After 2 weeks at room temperature the concentration of MEHP had increased to values b...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1978.tb02903.x
更新日期:1978-07-01 00:00:00
abstract::A non-blow-molded LE-2 polyolefin (PO) container was developed to store single-donor apheresis platelet concentrates (PCs) processed by the Haemonetics Plasma Collecting System (PCS) at 22 degrees C for over 1 day. We molded PO containers with heat-sealing of light-weight polymer alloy films with a thickness of 0.25 m...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1989.tb02022.x
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:In accordance with Food and Drug Administration (FDA) regulations, platelets can be stored in the liquid state at 22 degrees C for only 5 days. Platelets frozen with 6% dimethylsulphoxide (DMSO) can be stored at -80 degrees C for 2 years, and platelets frozen with 5% DMSO can be stored at -150...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2005.00592.x
更新日期:2005-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:This study was undertaken to provide denominator data relating to the timing and location of transfusion, to support interpretation of reports of incorrect blood component transfused (IBCT) events to the UK Serious Hazards of Transfusion (SHOT) scheme. MATERIALS AND METHODS:The study was carr...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2007.00952.x
更新日期:2007-10-01 00:00:00
abstract::Some effector functions of various immunoglobulin (IgG) preparations have been compared. Tests for opsonic activity were performed in vitro with human peripheral blood leukocytes and in vivo with mice. The augmentation effects on luminol-dependent chemiluminescence were investigated with human leukocytes. The compleme...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1986.tb01968.x
更新日期:1986-01-01 00:00:00
abstract::An alternative to blood transfusion, based on oxygen-carrying solutions, has been sought for over a century. The present 'first-generation' haemoglobin-products were based on observations that crosslinking with, for example, glutaraldehyde, overcame subunit dissociation and renal toxicity. Experience with these soluti...
journal_title:Vox sanguinis
pub_type: 杂志文章,评审
doi:10.1111/j.1423-0410.2006.00789.x
更新日期:2006-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The ultimate goal was to generate an industrial-scale process suitable to produce a high-yield, safe and stable immunoglobulin G (IgG) preparation for intravenous administration, which is ready to use for customer convenience. This new liquid 10% IgG preparation (IGIV 10%) was compared to Gamm...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2006.00846.x
更新日期:2007-01-01 00:00:00
abstract::A panel of 50 blood group antibodies covering a range of blood group antigens has been tested in the presence of 0.25% beta-propiolactone as a possible means of reducing infectivity of high-risk HTLV III/HBsAg samples. 11/50 (22%) antibodies could not be detected by the indirect antiglobulin test, and 6/40 (15%) were ...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1986.tb00207.x
更新日期:1986-01-01 00:00:00
abstract:OBJECTIVES:We have developed and optimised a new flow-cytometric method for the measurement of the Fc function of intravenous immunoglobulin (IVIg) preparations, which is important in predicting the effector function of immunoglobulin (Ig) in such preparations. MATERIALS AND METHODS:Ig was bound to a monocytic cell li...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1159/000031178
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:We identified some fatal cases where massively bleeding patients received inadequate transfusion therapy. The aim of this study was to review and evaluate the transfusion practice in acutely multitransfused patients. MATERIALS AND METHODS:Patients receiving > 10 units of red blood cells (RBC)...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2005.00668.x
更新日期:2005-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Pr1,2,3, PrM, Sa and Sia-l1, -b1, -lb1 are sialic acid (NeuNAc)-dependent antigens recognized by human cold agglutinins. Pr and Sa antigens are the O-glycans of glycophorins containing alpha 2,3NeuNAc (to galactose) and/or alpha 2,6NeuNAc (to galactosamine). The antigens of the Sia-l1, -b1, -l...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Platelet concentrates were prepared in plastic packs of polyvinyl chloride with tri(2-ethylhexyl) trimellitate as plasticizer. They were stored, with gentle shaking, at room temperature for periods up to 7 days before labelling with isotope and reinfusing. In vivo survival studies, platelet counts, pH and electron mic...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1982.tb00016.x
更新日期:1982-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Transfusion of fresh-frozen plasma is still a pillar in emergency medicine for using to prevent dilutional coagulopathy or disseminated intravascular coagulation after severe blood loss, but thawing procedures can delay its availability. On the other hand, the wastage of plasma, once thawed an...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.2004.00552.x
更新日期:2004-10-01 00:00:00
abstract::There is almost general agreement that removal of leukocytes from blood components reduces the incidence of HLA-antibody formation and refractoriness to random platelet transfusions. Recently filters have become available, which are able to reduce leukocyte contamination in platelet suspensions with acceptable platele...
journal_title:Vox sanguinis
pub_type: 杂志文章
doi:10.1111/j.1423-0410.1992.tb01214.x
更新日期:1992-01-01 00:00:00