Abstract:
:Although several chemical carcinogenes have been shown to induce malignant transformation in human cells in culture, the molecular events involved in conversion of normal cells to malignant cells remain unknown at present. Normal human cells seem to be resistant to transformation by a single oncogene unless these cells have been immortalized by chemical carcinogens or DNA tumor virus genes. In some human cell systems, malignant phenotype is expressed after activation of protooncogenes probably through the mechanism of point mutations. Our results represent the first induction of KRAS gene rearrangement in cells of patients with familiar polyposis coli (FPC) treated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and 12-0-tetradecanoyl-phorbol-13-acetate (TPA). Whether or not KRAS rearrangement is a prerequisite for transformation in these cells is unknown. The contrast between the response of the FPC cells following treatment with these chemicals compared with that of normal fibroblasts suggests that KRAS gene rearrangement in FPC cells may be considered as a genetic marker for the disease. Studies of transformation with chemicals and oncogenes extended to nonhuman primate--marmoset cells have shown that two viral oncogenes, v-src and v-sis, converted marmoset cells to altered morphology, anchorage independence and immortality in cell culture. The transformed cells, however, lacked tumorigenicity in allogeneic and xenogeneic hosts. In v-src transformed cells additional changes associated with mutations of transforming virus and chromosome rearrangements were necessary for induction of tumorigenicity in allogeneic marmosets. Transformation of marmoset cells by chemical carcinogens depended on the type of target cells. Skin fibroblasts treated with MNNG acquired transformed morphology, anchorage independence and random chromosome aberrations. Kidney cells treated with the same carcinogen remained untransformed, but attained the same characteristics of transformation when exposed to TPA. Neither skin nor kidney transformed cells showed true tumorigenic potential in nude mice.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Khoobyarian N,Marczynska Bsubject
Has Abstractpub_date
1989-09-01 00:00:00pages
1367-76issue
5eissn
0250-7005issn
1791-7530journal_volume
9pub_type
杂志文章,评审abstract:AIM:To identify tissue biomarkers that are predictive of the therapeutic effect of sunitinib in treatment of metastatic clear cell renal cell carcinoma (mCRCC). MATERIALS AND METHODS:Our study included 39 patients with mCRCC treated with sunitinib. Patients were stratified into two groups based on their response to su...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:The altered expression of multidrug resistance 1 (MDR1) contributes to various types of carcinogenesis and their disease progression. Hypermethylation of CpG islands located in the promoter regions is involved in gene silencing at the transcriptional level. The methylation status of MDR1 gene promoter was in...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::We have developed a chemosensitivity assay of human tumors growing on collagen-sponge-gel-supported histoculture. This assay is thus termed the Histoculture Drug Response Assay (HDRA). In the HDRA, the end points of [3H]thymidine incorporation measured by histological autoradiography and tetrazolium dye reduction were...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract::The in vitro antiproliferative or stimulatory activity of an aqueous mistletoe extract (AME) with a defined content of bioactive mistletoe lectin (ML) was investigated in 6 human tumor cell lines, including two melanomas and leiomyosarcomas, each of which had previously been reported to show evidence of growth stimula...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract:BACKGROUND:Malignant melanoma is a life-threatening disease with a poor prognosis due to its capacity for rapid metastasis and resistance to radio- and chemo-therapy. A pharmacological compound, CP-31398, was recently found to be able to stabilize wild-type p53 and rescue mutant p53 to enhance its transcriptional activ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:The mitochondrial protein translocase of inner mitochondrial membrane 17 homolog A (TIMM17A) has been identified as a biomarker of breast cancer. The present study aimed to investigate the biological role of TIMM17A in human breast cancer cells. MATERIALS AND METHODS:Anti-TIMM17A transgenes were stably tran...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:The determination of dihydropyrimidine dehydrogenase (DPD) deficiency is important in avoiding severe 5-fluorouracil (FU) toxicity. The dihydrouracil (UH2)-uracil (Ura) ratio (UH2/Ura) in plasma might be an important indicator of the risk of 5-FU catabolic deficiency. In order to clarify this possibility, th...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-09-01 00:00:00
abstract:BACKGROUND/AIM:Oxysterol plays important physiological roles in diverse biological processes including apoptosis. However, the mechanisms underlying oxysterol-induced apoptosis remain unknown. 25-hydroxycholesterol (25-HC) is an oxysterol synthesized by cholesterol 25-hydroxylase from cholesterol during sterol metaboli...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.14009
更新日期:2020-02-01 00:00:00
abstract::Binding of 20-[3H]-phorbol 2, 13-dibutyrate [( 3H] PDB) to intact human K 562 cells was characterized. Specific binding of [3H] PDB to K 562 cells at 20 degrees C or 37 degrees C reached a maximum within 15-20 min. Maximal specific [3H] PDB binding to K 562 cells was followed by a decline (down regulation) of radioact...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
abstract:BACKGROUND:F-Box protein 45 (FBXO45) is reported to be associated with cancer aggressiveness. We investigated the relationship between FBXO45 and clinicopathological factors in gastric cancer (GC). MATERIALS AND METHODS:We used immunohistochemistry to investigate FBXO45 expression in 104 GC samples; the prognostic val...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11305
更新日期:2017-01-01 00:00:00
abstract::A new treatment is proposed for mice bearing a Krebs ascitic tumor targeting on both the malignant cells and the host cells having the "cancer functioning" (cancer cells) (Anticancer Res 9: 941-946 1989). It is shown that the treatment (a) injure specifically via 64Cu transmutation the DNA of the malignant cells and f...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1989-07-01 00:00:00
abstract:AIM:The aim of this study was to evaluate the ability of tissue polypeptide-specific antigen (TPS), carcinoembryonic antigen (CEA), and cancer antigen 15-3 (CA 15-3) to predict relapse in breast cancer patients, when the measurement of biomarkers is performed within 6 months after surgery. PATIENTS AND METHODS:Four hu...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12245
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Neutral endopeptidase 24.11 (NEP) is a cell-surface peptidase that inactivates a variety of neuropeptide substrates. In addition to catalytic activity, NEP can exert biological effects through protein-protein interactions. We previously reported that NEP directly associated with tyrosine-phosphorylated Lyn k...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract:BACKGROUND/AIM:Anaplastic thyroid cancer (ATC) is one of the most aggressive human malignancies, remaining generally incurable. Histone deacetylase (HDAC) seems to play a role in regulating transcription of genes involved in ATC, making HDAC inhibitors (HDACI) promising anticancer drugs for ATC. The purpose of this rev...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:10.21873/anticanres.13220
更新日期:2019-03-01 00:00:00
abstract:BACKGROUND:Basal cell carcinomas (BCCs) are the most common cutaneous tumors, accounting for approximately 70% of all malignant skin tumors. A morphometric analysis was performed to evaluate whether morphometric parameters of the nuclei of BCC tumor cells may serve as predictors for biological behavior like recurrence ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
abstract::The purpose of these experiments was to test whether a brief course of insulin-like growth factor-1 (IGF-1) injection (escalating doses) concomitant to irradiation ameliorates radiation-induced kidney dysfunction and lethal bowel toxicity in a mouse model of unilateral high-dose irradiation of the kidney and adjacent ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract:UNLABELLED:Malignant pleural mesothelioma is a rare tumour. A three-modal strategy, comprising of surgery, radiotherapy and chemotherapy has been shown to be essential for appropriate management. Current literature evidences the importance of radiation therapy in the adjuvant setting for local control of the disease, a...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-04-01 00:00:00
abstract::The retinoblastoma suppressor (Rb)-associated protein 46 (RbAp46) is a nuclear protein of 46 kDa and contains four repeats that end with Trp-Asp (WD) residues. In this study, we reveal that the RbAp46 protein level upon SUMO-1 expression was increased. The increasing level of RbAp46 protein by SUMO-1 was not regulated...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2008-11-01 00:00:00
abstract:BACKGROUND/AIM:The aim of this study is to find synergistic effect using FH535 and sorafenib by targeting the RAS/RAF/MAPK and WNT/β-catenin pathways. MATERIALS AND METHODS:3H-Thymidine incorporation assays were performed to address Huh7 and liver cancer stem cell (LCSC) inhibition using FH535 and sorafenib, alone and...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-04-01 00:00:00
abstract:BACKGROUND:Interleukin 32 (IL32) is an intracellular pluripotent cytokine produced by epithelial cells, monocytes, T-lymphocytes and natural killer cells and seems to be involved in the pathogenesis of cancer and inflammatory diseases. Our purpose was to assess the role of protein expression and genetic polymorphisms o...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12225
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:The cisplatin-pemetrexed and cisplatin-gemcitabine combinations are considered the standard treatment for malignant pleural mesothelioma. The purpose of this study was to examine the efficacy of gemcitabine plus docetaxel in the first-line setting, as this combination has not been investigated in mesotheliom...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-08-01 00:00:00
abstract:BACKGROUND:The association of TN expression, tumour grading and expression of commonly used histopathologic prognostic factors (p53 and estrogen receptor) was examined in 62 cases of invasive ductal carcinoma of the breast. MATERIAL AND METHODS:Following histological grading immunohistochemical reactions were undertak...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:While procarbazine with 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (PC) added to vincristine (PCV) was proven beneficial in the treatment of co-deleted anaplastic oligodendroglioma (AO), the question of whether PC alone is sufficient is important, as vincristine adds toxicity with uncertain benefit. This r...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-10-01 00:00:00
abstract::In the clinical setting, administration of high daily or bolus doses of vitamin D is often solely based on 25-hydroxyvitamin D [25(OH)D] testing. This review summarizes the evidence of the effect of vitamin D on cardiovascular disease (CVD). Meta-analyses of randomized controlled trials (RCTs) have demonstrated that C...
journal_title:Anticancer research
pub_type: 杂志文章,meta分析,评审
doi:10.21873/anticanres.13643
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND/AIM:KRAS and BRAF are two genes commonly mutated in colorectal cancer (CRC). Even though BRAF is a downstream target of KRAS in the MAPK signalling pathway, KRAS- and BRAF-mutated CRCs are found to display several different clinical and histopathological features. We investigated whether a differential expre...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12272
更新日期:2018-02-01 00:00:00
abstract::Tumor cell invasion was studied using the 9-day chick embryo skin (CES). The invasion process was broken down into 4 sequential events: adhesion, penetration, proliferation and colonization. Tumor cells used in this study included cells from various human and canine solid tumors, tumor cell lines from human and animal...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract:PURPOSE:To further evaluate lung cancer risk associated with rare Hras1 VNTR alleles and possible biological mechanisms. MATERIALS AND METHODS:The Hras1 VNTR was genotyped in 295 lung cancer patients and 500 healthy controls by PCR and high resolution electrophoresis. Microsatellite alterations were examined in 168 tu...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) is a food mutagen which has been found to be carcinogenic in the Fischer-344 rat. We have assessed the effects of a diet containing menhaden oil on IQ-DNA adduct formation in several organs of the male Fischer-344 rat. Young adult, male Fischer-344 rats were maintained on e...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract:BACKGROUND:Intradermal or oral administration of lipopolysaccharide derived from Pantoea agglomerans (IP-PA1) has shown prophylactic and antitumor effects without serious side-effects. While it is known that tumor necrosis factor (TNF)-alpha produced by activated macrophages plays an important role in the expression me...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-11-01 00:00:00
abstract:AIM:To report the outcome of radiation therapy (RT) +/- chemotherapy in HIV-seropositive patients with Head and Neck Squamous Cell Carcinoma (HNSCC). PATIENTS AND METHODS:This is the largest single-Institution retrospective study to date, consisting of 73 HIV patients with HNSCC treated from January 1997-2010. The med...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2013-12-01 00:00:00