Abstract：
:The in vitro antiproliferative or stimulatory activity of an aqueous mistletoe extract (AME) with a defined content of bioactive mistletoe lectin (ML) was investigated in 6 human tumor cell lines, including two melanomas and leiomyosarcomas, each of which had previously been reported to show evidence of growth stimulation if treated with low concentrations of isolated ML. The effects of AME were compared to that of the standard cytotoxic agent adriamycin (ADR) using the well established propidium iodide and sulforhodamin B proliferation assays. The AME concentrations used ranged from 0.5 pg to 5 ng (0.82 fMol-85 pM) bioactive ML/ml in melanoma (HT-144, SK-MEL-28) and leiomyosarcoma (SK-MLS-1, S-UT-1B) cell lines and from 0.1-100 ng ML/ml (1.7 pM-1.7 nM) in MCF-7 breast cancer and SW620 colon carcinoma cell lines, respectively. The influence of AME on cell growth was determined at various time-points from 24 hours to 6 days of exposure. We found a time- and cell line-dependent inhibition of tumor cell growth, but no reproducible stimulation of tumor cell proliferation. Inhibitory concentrations 50% (IC50) for e.g. the SK-MEL-28 melanoma cell line, decreased from 4.1 ng ML/ml at 24 hours to 0.16 ng ML/ml at 72 hours and 0.18 ng ML/ml at 5 days. Our data clearly demonstrate that, by applying scientifically valid methods and procedures, the standardized AME did not stimulate tumor cell proliferation but showed time- and concentration-dependent antiproliferative effects.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Burger AM,Mengs U,Kelter G,Schüler JB,Fiebig HHsubject
Has Abstractpub_date
2003-09-01 00:00:00pages
3801-6issue
5Aeissn
0250-7005issn
1791-7530journal_volume
23pub_type
杂志文章abstract：:Radiation therapy is essential for local tumor control for many types of cancer histologies. Technological advancements in recent years have allowed for precise irradiation of target tissues while minimizing the dose to non-target tissues. To enhance radiation damage to cancer cells and further limit the radiation eff...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2015-05-01 00:00:00
abstract：AIM:The purpose of the present study was to identify variables associated with high risk of failure in patients with locally advanced squamous cell carcinoma of the oral cavity (SCC-OC). PATIENTS AND METHODS:This retrospective study included 191 patients with stage III-IVb SCC-OC treated with post-operative radiothera...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-02-01 00:00:00
abstract：AIM:To evaluate the tolerability and efficacy of oxaliplatin-based chemotherapy (OBC) in patients ≥ 75 years old with metastatic colorectal cancer (CRC). PATIENTS AND METHODS:We reviewed the medical records of 126 patients with unresectable stage IV CRC in terms of OBC administered as first-line chemotherapy whenever ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-10-01 00:00:00
abstract：:A new cell line, designated CC2/CUHK2, was established from a squamous carcinoma of the uterine cervix from a Chinese patient. The cell line grew well without interruption for over 12 months and over 90 passages. The doubling time of CC2/CUHK2 was 72 hours at passage 57. When seeded at a density of 1.5 x 10(3) cells p...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：:The effects of two gallium (Ga) compounds, Ga chloride (GaCl3) and tris(8-quinolinolato)Ga (III) on the viability of A549 human malignant lung adenocarcinoma cells were investigated. The results demonstrated that both drugs reduced the viability of A549 cells but to different extents. The inhibitory effects of tris(8-...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：BACKGROUND:To evaluate the outcome and discover predictive factors for patients with stage IVA cervical cancer treated with definitive radiation therapy. PATIENTS AND METHODS:We retrospectively reviewed 34 patients with stage IVA cervical cancer who received definitive radiation therapy between 1992 and 2009. RESULTS...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
abstract：:Evidence exists that estrogen metabolism has a role in the pathogenesis of recurrent respiratory papillomatosis (RRP). This disease has a papillomavirus etiology and is characterized by recurrent benign tumors with a significant propensity to become malignant. We have measured the systemic transformation of estrogen u...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：:Adenomas of the stomach are histologically classified into tubular and villous adenomas, mixed (tubulovillous) and papillary phenotypes being also recognized. They are localized in the antrum and the body. In a previous work we reported a case of serrated adenoma of the cardia having, at the time of diagnosis, an inva...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-05-01 00:00:00
abstract：:We have studied the effects of PAI-1 on the formation of metastasis using human fibrosarcoma cells and DNA transfection. A clone (1-3C), which shows low constitutive expression of PAI-1 and low metastatic potential to the lung, was selected from human fibrosarcoma cell line HT-1080. Newly-derived clones transfected wi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND:The prognostic significance of mutant p53 protein, p-glycoprotein (P-gp) and glutathione S-transferase-pi (GST-pi) in patients with unresectable non-small cell lung cancer (NSCLC) has not been established. MATERIALS AND METHODS:We performed immunohistochemical staining of p53, P-gp and GST-pi using transbro...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：UNLABELLED:The aim this study was to assess the efficacy of cisplatin-epirubicin-vinorelbine, as primary chemotherapy, in reducing the tumour burden in T2-3 N0-2 breast carcinomas. Breast conservative surgery (BCS) rate, clinical and pathological complete response (pCR), toxicity and 5-year disease-free survival (DFS) ...
journal_title：Anticancer research
pub_type： 杂志文章,多中心研究
doi：
更新日期：2007-07-01 00:00:00
abstract：:An in vitro pilot study was conducted of the possible utility of combining low doses of cytosine arabinoside (araC) and GM-CSF to stimulate leukemic cell differentiation. Cytosine arabinoside induced macrophage differentiation in some cultures but only when the drug produced substantial cytostatic effects. GM-CSF, whe...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
abstract：:Three sublines have been derived from the parental line Mv1Lu by transfection with normal and mutated Ha-ras, and myc oncogenes, and subsequent cloning. All the oncogenes have increased the growth rate of the cell in vitro, increased their plating efficiency in monolayer and suspension, and reduced their serum depende...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:To determine the changes in biological features of the prostate during the course of local recurrence of prostate cancer after endocrine therapy, histologic grade, proliferating activity and apoptotic indices were examined in prostate specimens obtained before treatment and at recurrence. A total of 16 patients, who h...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：BACKGROUND:Trastuzumab is used for the adjuvant (postoperative) treatment of (HER2)-positive early breast cancer. The duration of treatment is set at one year. The goal of our study was to examine the effective duration of trastuzumab treatment in routine clinical practice. PATIENTS AND METHODS:We performed a retrospe...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-10-01 00:00:00
abstract：:We report a 64-year-old woman with a 9-cm liver tumor in the left lateral section. The patient had neither hepatitis B or C virus infection, nor cirrhosis. Carbohydrate antigen 19-9 (CA 19-9) level was 1,889 U/ml. We also suspected bulky hilar lymph node metastasis, and a left lateral sectionectomy without lymph node ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13327
更新日期：2019-04-01 00:00:00
abstract：AIM:To elucidate the behavior of 1 phosphatidylinositol 4,5 bisphosphate (PIP2) 5 phosphatase (PIP2 5-Pase, EC 3.1.3.36) and 1 phosphatidylinositol 4 phosphate (PIP) 4-phosphatase (PIP 4-Pase) in cancer cells, the steady state activities of PIP2 5-Pase and PIP 4-Pase were determined in the particulate fractions of rat ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-11-01 00:00:00
abstract：BACKGROUND:The major side-effects of bevacizumab in glioma treatment are venous thromboembolic events (VTE). We retrospectively evaluated factors potentially predictive of thromboembolic events. PATIENTS AND METHODS:Bevacizumab, alone or in combination with chemotherapy was used as salvage therapy for recurrence in ma...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-05-01 00:00:00
abstract：BACKGROUND/AIM:To evaluate the effectiveness of enzalutamide in Italian patients with hormone-refractory metastatic castration-resistant prostate cancer, progressing after chemotherapy with docetaxel plus prednisone. PATIENTS AND METHODS:A total of 60 patients were enrolled. Reduction in serum prostate-specific antige...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：10.21873/anticanres.11472
更新日期：2017-03-01 00:00:00
abstract：:We investigated the effects of an exogenous cdk inhibitor, butyrolactone-I, on cell growth inhibition, apoptosis induction, and the regulation of apoptosis in pancreatic cancer cells with mutated p53. Cell growth was dose-dependently inhibited by Butyrolactone-I in PANC-1 and AsPC-1 cells. Phosphorylation of pRb and C...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：BACKGROUND:Copper transporters (CTR) also regulate the cellular transport of platinum drugs, but their role in platinum resistance of ovarian cancer has not been elucidated. MATERIALS AND METHODS:CTR expression in ovarian cancer tissues resected from patients treated by platinum-based chemotherapy was evaluated immuno...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
abstract：BACKGROUND:The efficacy and toxicity of adjuvant chemo-immunotherapy using dendritic cells and activated killer cells are not clear in post-surgical primary lung cancer patients. PATIENTS AND METHODS:Pathologically diagnosed N2 lung cancer patients were selected for postsurgical adjuvant chemo-immunotherapy. The activ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-03-01 00:00:00
abstract：BACKGROUND:Basal cell carcinomas (BCCs) are the most common cutaneous tumors, accounting for approximately 70% of all malignant skin tumors. A morphometric analysis was performed to evaluate whether morphometric parameters of the nuclei of BCC tumor cells may serve as predictors for biological behavior like recurrence ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：:In vivo investigations on oncogene action may provide new findings on the toxicology of potential carcinogens. In this study we investigated the early effects of different cytostatic protocols on early oncogene expression in CBA/Ca mice. Most of the examined protocols showed detectable early changes, especially those ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:Elevated levels of hypoxia inducible factor-1 (HIF1) are linked to tumor metastasis, angiogenesis, poor patient prognosis and response to chemotherapy. HIF1α is a master regulator of the hypoxic response, including in cancer cells, through transcriptional activation of several target genes. Previously, we identified c...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-07-01 00:00:00
abstract：BACKGROUND:During the progression from low-grade dysplastic nodule (DN) to progressed hepatocellular carcinoma (HCC), intranodular portal tracts gradually disappear, while unpaired arteries develop increasingly. Contrast-enhanced ultrasound (CEUS) is highly accurate in depicting intranodular vascularity. This study eva...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-11-01 00:00:00
abstract：:Pro- and anti-apoptotic factors and intracellular signaling pathways are targets for therapeutic development of anticancer agents. Carboxyamido-triazole (CAI) is an inhibitor of transmembrane calcium influx and intracellular calcium-requiring signal transduction pathways. The present study investigates the effects of ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-09-01 00:00:00
abstract：:Various lignified materials, including pine cone extract and a commercial lignin, stimulated the morphological change (spreading) of mouse peritoneal macrophages, and their functional maturation as judged by increase in NBT-reducing activity. When these materials were treated with NaClO2 to decompose the phenolic stru...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
abstract：BACKGROUND/AIM:The toxicity of the proteasome inhibitor MG132 was tested alone and combined either with the topoisomerase I inhibitor topotecan or the topoisomerase II inhibitor etoposide against a panel of 18 cell lines representing six pediatric tumor types. MATERIALS AND METHODS:MG132, topotecan, etoposide and thei...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12684
更新日期：2018-07-01 00:00:00
abstract：BACKGROUND:A cross-sectional study was performed on a cohort of colorectal cancer (CRC) patients to reveal any influence of age, gender, and subsite on grades of malignancy. PATIENTS AND METHODS:Data from histopathological grading according to WHO criteria were pooled into groups of low-grade (well and moderately diff...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-09-01 00:00:00
