Abstract：
BACKGROUND:Malignant melanoma is a life-threatening disease with a poor prognosis due to its capacity for rapid metastasis and resistance to radio- and chemo-therapy. A pharmacological compound, CP-31398, was recently found to be able to stabilize wild-type p53 and rescue mutant p53 to enhance its transcriptional activity and suppress tumor growth in mice. Since many chemotherapeutic drugs induce p53-dependent apoptosis, we sought to investigate if CP-31398 would enhance chemosensitivity in human melanoma cells by stabilizing p53. MATERIALS AND METHODS:Human melanoma cell lines carrying either wild-type or mutant p53 were treated with CP-31398 and anticancer drugs, and the rate of cell survival or apoptosis was determined by sulforhodamine B, propidium iodide staining and flow cytometry. The p53 protein levels were determined by Western blotting. RESULTS:A non-toxic dose of CP-31398 elevated p53 protein levels in a wild-type (MMRU) and a mutant (Sk-mel-110) p53 melanoma cell line, but did not enhance cell death induced by camptothecin. Furthermore, pretreatment with CP-31398 did not enhance camptothecin-induced apoptosis or elevate p53 protein induction. In addition, pretreatment of MMRU with CP-31398 did not enhance cell death induced by cisplatin or vincristine. CONCLUSION:CP-31398 does not enhance chemosensitivity in human melanoma cells.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Luu Y,Li Gsubject
Has Abstractpub_date
2003-01-01 00:00:00pages
99-105issue
1Aeissn
0250-7005issn
1791-7530journal_volume
23pub_type
杂志文章abstract：BACKGROUND:The pS2 protein is involved in the maintenance of the integrity of the gastrointestinal tract. In breast cancer pS2 can be demonstrated in at least half of the tumors and probably reflects the functional status of ER. Several features make it likely that pS2 is involved in growth regulation. PATIENTS AND ME...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：BACKGROUND:It is essential that cytotoxic T-lymphocytes (CTLs) recognize tumor-associated antigens (TAAs) and exhibit antitumor effects in immunological responses for tumor rejection. However, only a few cases with oral squamous cell carcinoma (OSCC) have been studied for these antitumor mechanisms. We established a ca...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11898
更新日期：2017-09-01 00:00:00
abstract：:Immunohistochemical expression of p53 protein was studied in FNA specimens of 20 breast ductal carcinomas, 20 fibroadenomas and 20 atypical ductal hyperplasia of the breast. Nine cases of breast carcinomas (45%), five fibroadenomas (25%) and four atypical ductal hyperplasia (20%) were found to be p53-immuno-positive. ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：BACKGROUND:The aim of this study was to analyse the Epstein-Barr virus (EBV) latency by detecting the EBV-associated latent small nuclear RNAs (EBER), in a group of biopsies from Spanish patients with diagnosed nasopharyngeal carcinoma (NPC). MATERIALS AND METHODS:NPC paraffin samples with the presence of EBV demonstr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：BACKGROUND/AIM:To clarify whether renal dysfunction affects the incidence of adverse events associated with oxaliplatin, the present study was designed to investigate the relationship between creatinine clearance (Ccr) and the incidence of oxaliplatin-related adverse events. PATIENTS AND METHODS:A total of 287 CRC pat...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13953
更新日期：2020-01-01 00:00:00
abstract：:Brain tumors were induced in inbred Fischer rats (F344) by administration of methylnitrosourea in the drinking water. One of the induced tumors, a pleomorphic glioma (78-219), was established in vitro and propagated as 78FR-G-219 permanent cell line. The tumorigenicity of the established line was investigated by intra...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:Caspase-3 is a key executioner cysteine protease involved in programmed cell death or apoptosis. A ribozyme to human caspase-3 was designed, tested by in vitro cleavage, and transfected into a drug-resistant variant (DLKP-A5F) of a human lung carcinoma cell line (DLKP). By both stable and transient transfection, this ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：BACKGROUND:The correlation between telomerase activity and antitumor effects was investigated in cell lines of human gastric (MKN-28, MKN-45, and MKN-74) and breast (T-47D, MCF-7, ZR75-1) cancers to evaluate the possibility of utilizing this enzyme to predict tumor response to chemotherapy. MATERIALS AND METHODS:After...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：BACKGROUND:This study evaluated the therapeutic efficacy of combining vascular disrupting agents with antiangiogenic agents. MATERIALS AND METHODS:Human clear cell renal carcinoma (Caki-1) tumors were established in nude mice. Treatments consisted of Avastin (2 mg/kg) administered twice a week; CA4P (100 mg/kg) or OXi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-07-01 00:00:00
abstract：:A high-throughput 32D(L858R/T790M) cell-based assay to identify inhibitors of the L858R/T790M mutant epidermal growth factor receptor (EGFR) pathway was established. After screening, ten hits from among 60,000 compounds in our in-house compound library were initially identified. In the secondary assays, one hit, 1-[2-...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:The most widely-known anti-tumor drugs often induce marked immunosuppression which can give rise to one or more sepses. Anti-infection measures immediately applied can sometimes prove largely ineffective or even useless, the patient dying not as a result of the spread of the tumour but as a direct consequence of oppor...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：BACKGROUND:Tumor budding and a proficient mismatch repair (pMMR) status are considered adverse prognostic factors in colorectal cancer (CRC). The aim of this pilot study was to assess tumor budding in primary CRC with pMMR versus that with deficient mismatch repair (dMMR). MATERIALS AND METHODS:Tumor budding was retro...
journal_title：Anticancer research
pub_type： 杂志文章,随机对照试验
doi：10.21873/anticanres.12778
更新日期：2018-08-01 00:00:00
abstract：:Allelic loss at specific chromosomal loci was demonstrated in carcinoma of the cervix (CaCx) obtained from Hong Kong Chinese by restriction fragment length polymorphism analysis. Altogether 12 patients with CaCx, 7 with cervical intraepithelial neoplasia (CIN) and 7 with normal cervical tissues were studied. Polymorph...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：:An increase in the 20q13 copy number has been reported in a wide range of solid tumors including breast cancers. However, the biological significance of this 20q13 gain has not yet been defined. We examined the 20q13 gain in breast cancer cells by dual-color FISH using two different DNA probes specific for the region ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：BACKGROUND/AIM:New anticancer drugs are usually tested on cancer cells in culture in a standard medium. We stimulated immune polynuclear cells by lipopolysaccharides to obtain an enriched medium (EM) containing inflammatory cytokines more closely reflecting the tumor microenvironment and tested a rhenium-diselenium (Re...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14146
更新日期：2020-04-01 00:00:00
abstract：:The presence of steroid receptors in breast cancer is usually considered as a good indicator for responsiveness to antiestrogen treatment. However, not all estrogen-receptor (ER) positive tumors respond to tamoxifen, probably owing to the natural heterogeneity of breast cancer, which after tamoxifen treatment can resu...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
abstract：BACKGROUND:The Notch inhibitors, γ-secretase inhibitors (GSIs), are promising candidates for molecular targeted therapy against leukemia. However, they show only limited effectiveness. We thought that the efficacy of GSIs might be improved by their combination with Hedgehog inhibitors and Wnt inhibitors because these s...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-03-01 00:00:00
abstract：BACKGROUND:Kita-Kyushu lung cancer antigen-1 (KK-LC-1) is a cancer/testis antigen and predominant target for cancer immunotherapy. Its detection is only established based on gene expression. In this study, we established a monoclonal antibody against KK-LC-1 to detect its protein expression in formalin-fixed samples. ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12937
更新日期：2018-10-01 00:00:00
abstract：BACKGROUND:In the present study, we examined the relationships between microsatellite status and Ki-67 labeling index, a proliferative marker, in colorectal cancers in order to clarify the common biological profiles of familial and sporadic cases of MSI (microsatellite inability)-positive tumors. PATIENTS AND METHODS:...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：BACKGROUND:Pure or metaplastic squamous cell carcinoma (SCC) of the breast is a rare entity with an unclear pathogeny and aggressive clinical behaviour. An attempt was made to characterize its differential immunohistochemical and biological profile. PATIENTS AND METHODS:Twenty-seven cases of SCC (pure or not) of the b...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：BACKGROUND:Immune cells (lymphocytes and macrophages) provide the microenvironment for immune surveillance of metastatic prostate cancer (PCa) cells in pelvic lymph nodes. We have hypothesized that degeneration and/or apoptosis of metastatic PCa cells in pelvic lymph nodes can distinguish between aggressive and non-agg...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-09-01 00:00:00
abstract：BACKGROUND:To clarify the pathogenicity of multiple human papilloma virus (HPV) infection, we applied SSCP (single-strand DNA conformation polymorphism) analysis for cervical neoplastic lesions. MATERIALS AND METHODS:Two hundred and sixty-six cervical swab specimens from normal cervix (n=64), cervical dysplasia (n=95)...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-05-01 00:00:00
abstract：:Lung cancer is the leading cause of cancer-related death worldwide, with approximately 1.2 million deaths annually. The standard-of-care in patients with advanced disease is platinum-based doublet chemotherapy. Recent advances in the understanding of biological mechanisms of tumor growth have allowed for identificatio...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2014-04-01 00:00:00
abstract：AIM:To evaluate the efficacy and toxicities of cisplatin and daily oral etoposide in patients with recurrent cervical cancer. PATIENTS AND METHODS:Treatment was initiated with oral etoposide 25 mg/day for 21 consecutive days, with intravenous cisplatin at 50 mg/m², on day 1, every 4 weeks, then the etoposide dose was ...
journal_title：Anticancer research
pub_type： 杂志文章,多中心研究
doi：
更新日期：2011-09-01 00:00:00
abstract：:A 60-year-old male patient with advanced gastric adenocarcinoma was treated by subtotal gastrectomy with dissection of the regional lymph nodes. Microscopic examination of the tumour revealed tubular adenocarcinoma which invaded the muscularis propria. There was no evidence of metastatic carcinoma in the dissected lym...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：:Germ line point mutations in the RET proto-oncogene have been implicated in four inherited disorders: multiple endocrine neoplasia 2A (MEN 2A) and 2B (MEN 2B); familial medullary thyroid carcinoma (FMTC); and Hirschprung's disease, a congenital lack of enteric plexus neurons. Oncogenically activated RET has also been ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：BACKGROUND:Nucleotide-binding domain and leucine-rich repeat-containing family, pyrin domain-containing 7 (NLRP7) (pyrin-containing apoptotic protease activating factor-1-like protein 3; PYPAF3, NACHT domain-, leucine-rich repeat, and pyrin domain-containing 7; NALP7) has been thought to contribute to innate immunity a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-07-01 00:00:00
abstract：:Since the discovery of cisplatin about 40 years ago, the design of innovative metal-based anticancer drugs is a growing area of research. Transition metal coordination complexes offer potential advantages over the more common organic-based drugs, including a wide range of coordination number and geometries, accessible...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2014-01-01 00:00:00
abstract：AIM:To analyze how patients experience Stereotactic guided vacuum-assisted breast biopsy (VABB) both physically and mentally. PATIENTS AND METHODS:Two hundred and eleven consecutive women underwent VABB using one of two different biopsy devices (ATEC® and Mammotome®). Patients were queried using a questionnaire. RESU...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-07-01 00:00:00
abstract：:Concomitant neoplasm and abdominal aortic aneurysm (AAA) is not a rare clinical association and can pose problems if antiblastic treatment is required. The literature shows lack of consensus owing to the fact that AAA can modify the liquid load and the hemodynamic setting so that chemotherapy toxicity profile becomes ...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1999-03-01 00:00:00
