Abstract：
BACKGROUND:It is essential that cytotoxic T-lymphocytes (CTLs) recognize tumor-associated antigens (TAAs) and exhibit antitumor effects in immunological responses for tumor rejection. However, only a few cases with oral squamous cell carcinoma (OSCC) have been studied for these antitumor mechanisms. We established a cancer cell line and autologous CTL pair for identifying TAAs in OSCC, despite difficulties in establishing such a pair. MATERIALS AND METHODS:A cell line (OTM) from a primary lesion and a CTL line (TcOTM) were derived from OSCC in a 64-year-old female patient. CTL clones were generated by repetitive limiting dilutions. Accurate characterization was performed by in vitro analysis. RESULTS:The TcOTM clone showed specific cytotoxic activity against OTM cells in an human leukocyte antigen (HLA)-A24-restricted manner. Furthermore, it exerted cytotoxicity against the allogenic HLA-A24 cell lines. CONCLUSION:These data indicated that the TcOTM clone recognized a TAA presented by HLA-A24 cells. This unique method will allow for identification of unknown TAAs for OSCC in the future.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Okamoto JY,Miyazaki A,Kobayashi JI,Miyamoto S,Sasaki T,Kanaseki T,Hirohashi Y,Torigoe T,Hiratsuka Hdoi
10.21873/anticanres.11898subject
Has Abstractpub_date
2017-09-01 00:00:00pages
4889-4897issue
9eissn
0250-7005issn
1791-7530pii
37/9/4889journal_volume
37pub_type
杂志文章abstract：BACKGROUND:Combination chemotherapy of irinotecan and amrubicin for advanced non-small cell lung cancer (NSCLC) has not been fully evaluated. To determine the maximum-tolerated dose (MTD), a phase I study in patients with advanced NSCLC was conducted. MATERIALS AND METHODS:Patients with stage IIIB/IV NSCLC were enroll...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2005-05-01 00:00:00
abstract：:We have previously found that in one of two human melanoma cell lines, potent increase in the polyamine catabolizing enzyme, spermidine/spermine N1-acetyltransferase (SSAT), correlate with growth sensitivity to the spermine analog, N1, N12-bis(ethyl) spermine (BESPM). Herein, we examine the generality of this SSAT res...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-07-01 00:00:00
abstract：:Some non-differentiation-induction benzo[a]phenothiazines and mutagenic benz[c]acridines more potently inhibited the mitogen-induced blast transformation of human-peripheral blood lymphocytes than differentiation-induction and non-mutagenic counterparts and phenothiazines. Differential absorption spectrophotometry rev...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：BACKGROUND/AIM:To evaluate the effectiveness of enzalutamide in Italian patients with hormone-refractory metastatic castration-resistant prostate cancer, progressing after chemotherapy with docetaxel plus prednisone. PATIENTS AND METHODS:A total of 60 patients were enrolled. Reduction in serum prostate-specific antige...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：10.21873/anticanres.11472
更新日期：2017-03-01 00:00:00
abstract：:The in vivo investigation of onco/suppressor gene effects may provide new information on chemical-environmental carcinogenesis. We previously described the elevation of onco/suppressor gene expression due to CHOP and COPP chemotherapeutical protocols in a CBA/Ca mouse model. Below we describe the results of the onco/s...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:The most active fraction (Fr. VI) of antitumor and antiviral pine cone extract was subjected to chemical structure analysis. IR, UV, and ESR spectroscopies, in addition to chromatographic analysis on cellulose-TLC plates, reveal that the major component of Fr. VI includes structures belonging to the lignin family. A c...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1989-11-01 00:00:00
abstract：BACKGROUND:Acquired resistance to platinum-based chemotherapy (Pt-chemo) is a major problem for improving the prognosis for patients with advanced epithelial ovarian cancer (EOC). However, the molecular mechanism of acquired resistance to Pt-chemo is not well understood. MATERIALS AND METHODS:hMLH1 promoter methylatio...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-05-01 00:00:00
abstract：BACKGROUND:The p53 tumor suppressor gene is altered in up to 70% of colorectal cancers. MATERIALS AND METHODS:We infected the colorectal cancer cell lines SW620 and KM12L4, in which p53 is mutated, with the replication-defective adenovirus Ad5/CMV/p53 to evaluate the effects of adenovirus-mediated wild-type p53 gene t...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：BACKGROUND/AIM:We evaluated the usefulness of reverse transcription-polymerase chain reaction (RT-PCR) for detecting anaplastic lymphoma kinase (ALK) translocations using cytology samples from lung cancer patients. MATERIALS AND METHODS:We analyzed ALK translocations by RT-PCR in cytology samples from lung cancer pati...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11696
更新日期：2017-06-01 00:00:00
abstract：:We studied a consecutive series of 54 cases of lower lip squamous cell carcinoma (LLSCC) in order to identify any variables which might predict the development of lymph node metastases. The cases were divided into 38 tumors without metastases (group I) and 16 tumors with lymph node metastases (group II). The following...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：OBJECTIVE:Laboratory rats can develop benign or malignant tumors (TM) spontaneously or following various carcinogenic processes, e.g. irradiation. The effects of irradiation vary according to the irradiation field (RF), the dosage and the strain of rat. Radiation-induced malignant TM in rats are predominantly sarcomas....
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：BACKGROUND:The potential of MTp53 knockout by oligodesoxyribonucleotide phosphothioates (ODNs) to affect proliferation, apoptosis and chemosensitivity in undifferentiated thyroid cancer (UTC) cells with a recessive MTp53 mutation was evaluated. MATERIALS AND METHODS:Transient transfections with ODNs complementary to p...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-03-01 00:00:00
abstract：:Two different techniques [reduction of a tetrazolium derivative (MTT) and 3HTdR uptake assays] were compared in order to evaluate the cytotoxic effects of different chemotherapeutic agents in vitro. The cytotoxicities of Melphalan, hexamethylmelamine and seven derivatives, and daunorubicin were measured on P388D1 mous...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:Glutathione (GSH) concentration is high in most tumour cells and this may be an important factor in resistance to chemotherapy. Previous in-vitro and animal experiments have shown a differential response of tumour versus normal cells to various cysteine delivery systems. More specifically, an in-vitro assay showed tha...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：BACKGROUND:Disseminated tumor cells (DTCs) in bone marrow (BM) occur in 30-40% of primary breast cancer patients. An impaired bone microenvironment may lead to reduced bone density and osteoporosis affecting the BM as a homing site for DTCs. The bone mineral density (BMD) and its correlation to DTC in BM was evaluated....
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-12-01 00:00:00
abstract：:Between 1975 and 1982, 202 previously untreated patients with multiple myeloma (MM) and clinical evidence of disease progression received intensive chemotherapy every 3-5 weeks to induce remission. Treatment regimen included intermittent courses of a combination of cycle non-specific drugs (melphalan, cyclophosphamide...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：BACKGROUND:While efficient surgical treatment is the key to prolonged survival of patients with colorectal cancer, post-surgical follow-up is important for the early detection of relapsing disease or of disease progression. Current dispensarization, typically based on imaging CT, PET, MR, is frequently supported by the...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-05-01 00:00:00
abstract：:The retinoblastoma suppressor (Rb)-associated protein 46 (RbAp46) is a nuclear protein of 46 kDa and contains four repeats that end with Trp-Asp (WD) residues. In this study, we reveal that the RbAp46 protein level upon SUMO-1 expression was increased. The increasing level of RbAp46 protein by SUMO-1 was not regulated...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-11-01 00:00:00
abstract：BACKGROUND:Cervical cancer prevention is performed by taking Pap smears. The correct execution of the smear is crucial as an inadequate smear might result in underdiagnosis. The second means of cervical cancer prevention is visual inspection of the cervix uteri with acetic acid, while often both methods are combined. W...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：:Chloroform (CHCl3) is an established rodent carcinogen and a prevalent contaminant of chlorine-disinfected drinking water. Thus in the United States CHCl3, along with other trihalomethanes, is regulated not to exceed 100 ppb in potable water. Recently, several studies have shown that CHCl3 also has anti-cancer propert...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-03-01 00:00:00
abstract：:The effects of the 18 carbon chain length series of fatty acids on the viability of a 3T6 derived mouse fibroblast cell line were tested. An overall concentration dependent cytotoxic effect was demonstrated with the saturated fatty acid, and cis- and trans- mono- and dienoic fatty acids; however, within a narrow range...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:The p53 gene product suppresses both tumor cell growth and the cellular transformation process promoted by oncogenes. Although several genes are known to be positively regulated by p53, it is unclear how many gene expression events are involved in p53-mediated growth arrest and apoptosis. Two-dimensional gel electroph...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-11-01 00:00:00
abstract：:The generation of MHC-unrestricted cytotoxicity of highly enriched human CD3+ T cells (95-99%) by treatment with IL-2 and/or anti-CD3 antibodies was studied. T cells obtained by positive immunomagnetic sorting (anti-CD3) developed comparable specific cytotoxicities against K562 and Daudi cells when cultured with IL-2 ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：AIM:To determine the effectiveness of postoperative concurrent daily low-dose cisplatin-based chemoradiation (CCRT) in patients with high-risk cervical cancer. PATIENTS AND METHODS:Patients with stage IB, IIA, or IIB cervical cancer who were initially treated with radical hysterectomy and pelvic lymphadenectomy, and w...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-06-01 00:00:00
abstract：:Norcantharidin[3], the demethylated product of cantharidin[1] has been used for the treatment of hepatoma, carcinomas of esophagus and gastric cardia, leukopenia and hepatitis. Since the enzyme xanthine oxidase is involved in the diseases mentioned above, and the reactive oxygen species produced by the enzyme induces ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
abstract：BACKGROUND/AIM:Neuroblastoma is a pediatric solid tumor refractory to eradication by chemotherapy. To determine whether Aloe vera (AV), a pontial anticancer reagent, could be useful in neuroblastoma therapy, we investigated the anti-proliferative effects of an AV protein extract. MATERIALS AND METHODS:Human neuroblast...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-08-01 00:00:00
abstract：:Ten patients with advanced renal cell carcinoma were given combined chemoendocrine treatment with tegafur and tamoxifen. Patients were orally administered 800 mg/body tegafur and 20 mg/body tamoxifen daily. One patient was assessed as CR, three as PR, two as NC and four as PD. One out of two patients with estrogen rec...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:This study was designed to determine whether circulating cancer cells in patients with gastric cancer can be identified by a RT-PCR method and to assess the clinical significance of the hematogenic dissemination of cancer cell in gastric cancer patients. A total of 41 patients with gastric cancer were enrolled in this...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：:35 patients suffering from malignant stage III/IV glioma were enrolled into a prospectively randomized clinical trial. All patients were provided with standard oncologic treatment (neurosurgery, radiation, basic clinical care according to protocol and indication) and randomly divided into a) treatment group: receiving...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1996-11-01 00:00:00
abstract：:cis-Diamminedichloroplatinum(II) (CDDP) is the most active anticancer agent. It has been reported that intracellular accumulation of CDDP is an important step as a determinant for resistance to CDDP, which may be modulated by Na+, K(+)-ATPase activity. In this study, the significance of membrane Na+, K(+)-ATPase activ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
