Abstract:
BACKGROUND:While efficient surgical treatment is the key to prolonged survival of patients with colorectal cancer, post-surgical follow-up is important for the early detection of relapsing disease or of disease progression. Current dispensarization, typically based on imaging CT, PET, MR, is frequently supported by the observation of tumour markers (CEA, CA19-9). Due to their limited sensitivity and selectivity, better tools for monitoring of the disease are desirable. Tumour cell-free DNA (cfDNA) has been recently demonstrated as a new promising molecular marker for observation and early detection of disease progression. PATIENTS AND METHODS:We present results of post-surgical monitoring tumour cfDNA in the cases of seven patients suffering from advanced forms of CRC. We applied a mutation-based approach in which the total cfDNA was screened for a specific somatic mutation present in the primary tumour. We screened a panel of the most frequent somatic mutations covering the genes APC, KRAS, TP53, PIK3CA and BRAF. All patients were tested positive for tumour cfDNA prior to surgery. cfDNA was then evaluated within a week after surgery and subsequently in monthly intervals. RESULTS:We present typical cases of colorectal cancer patients who underwent surgical treatment at different levels of radicality with or without adjuvant chemo/biotherapy. The tumour cfDNA status was found to be always closely correlated with the actual clinical status of the patient. CONCLUSION:The cfDNA appears to be a viable tool for the monitoring of the clinical progression of CRC in patients with cfDNA positivity prior to surgery.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Levy M,Benesova L,Lipska L,Belsanova B,Minarikova P,Veprekova G,Zavoral M,Minarik Msubject
Has Abstractpub_date
2012-05-01 00:00:00pages
1621-6issue
5eissn
0250-7005issn
1791-7530pii
32/5/1621journal_volume
32pub_type
杂志文章abstract::Serially propagated fibroblast clones were treated with 25-mM methionine and a thioguanine resistance assay was performed at each passage to measure mutagenesis at the HGPRT locus. The spontaneous frequency of thioguanine resistant mutants before treatment was less than 5 X 10(-6) in four clones and 22 X 10(-6) in one...
journal_title:Anticancer research
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abstract::Low concentrations of Adriamycin (ADM) do not inhibit the oxygen consumption of rat liver mitochondria because of the inability to cross the outer membrane. The involvement of this membrane as a permeability barrier is demonstrated by the results with exogenous ferrocytochrome c. ADM does not affect the basal rate of ...
journal_title:Anticancer research
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journal_title:Anticancer research
pub_type: 杂志文章
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更新日期:2006-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2005-07-01 00:00:00
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doi:
更新日期:2013-08-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
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doi:
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:2008-03-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
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更新日期:2010-10-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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journal_title:Anticancer research
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