Abstract：
BACKGROUND/AIM:We evaluated the usefulness of reverse transcription-polymerase chain reaction (RT-PCR) for detecting anaplastic lymphoma kinase (ALK) translocations using cytology samples from lung cancer patients. MATERIALS AND METHODS:We analyzed ALK translocations by RT-PCR in cytology samples from lung cancer patients diagnosed at the Nippon Medical School Hospital between 2013 and 2015. Immunochemistry (IHC) and break-apart fluorescence in situ hybridization (FISH) were also performed on available tissue samples. RESULTS:A total of 155 cytology samples were analyzed in our study. We obtained 115 (68%) samples from bronchial lavage. We were able to determine 153 (99%) results by RT-PCR with 4 (3%) positive samples. The four samples positive by RT-PCR were also positive by IHC and FISH performed on the tissue samples collected simultaneously. CONCLUSION:RT-PCR is a suitable method for detecting ALK translocations using cytology samples from patients with primary lung cancer, especially when tissue samples are not available.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Nakamichi S,Seike M,Miyanaga A,Chiba M,Matsuda K,Kobayashi K,Takahashi A,Takeuchi S,Minegishi Y,Kubota K,Gemma Adoi
10.21873/anticanres.11696subject
Has Abstractpub_date
2017-06-01 00:00:00pages
3295-3299issue
6eissn
0250-7005issn
1791-7530pii
37/6/3295journal_volume
37pub_type
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journal_title：Anticancer research
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doi：
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journal_title：Anticancer research
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