Abstract：
BACKGROUND:To clarify the pathogenicity of multiple human papilloma virus (HPV) infection, we applied SSCP (single-strand DNA conformation polymorphism) analysis for cervical neoplastic lesions. MATERIALS AND METHODS:Two hundred and sixty-six cervical swab specimens from normal cervix (n=64), cervical dysplasia (n=95), carcinoma in situ (n=79) and cervical cancer (n=28), were studied by nested PCR-SSCP analysis using L1 consensus primers. RESULTS:In 95 samples of cervical dysplasia, HPV infection was detected in 98.9% (94 out of 95), multiple HPV infection was detected in 38.3% (36 out of 94). In 19 squamous cell carcinomas (SCC) and 9 adenocarcinomas, the detection rate of HPV infection was 84.2% (16 out of 19) and 55.6% (5 out of 9), respectively, and all HPV-positive cases showed infection of a single HPV, among which HPV 16 occupied 68.6% (11 out of 16) in SCC and HPV 18 occupied 100% (5 out of 5) in adenocarcinoma. CONCLUSION:Multiple HPV infections may be concerned with pathogenicity in cervical dysplasia; however, the single infection with only a few HPV types, such as type 16 in SCC and type 18 in adenocarcinoma, may play a role in cervical carcinogenesis.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Nakagawa H,Sugano K,Fujii T,Kubushiro K,Tsukazaki K,Nozawa Ssubject
Has Abstractpub_date
2002-05-01 00:00:00pages
1655-60issue
3eissn
0250-7005issn
1791-7530journal_volume
22pub_type
杂志文章abstract：:Recently, it has been reported that total clearance (CLtot) of vancomycin is significantly higher in patients with malignancies compared to those without malignancies. In the present study, to clarify the mechanism of this enhancement in malignancy, we adopted rat animal models, using chemical carcinogen-induced osteo...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：:The aim of this study was to determine a possible risk of malignancies in the haematopoietic tissues of rats treated with styrene, either by injection or by inhalation. Two experiments were carried out: in the first (acute treatment), 12 male rats were treated intraperitoneally with different doses of styrene (40 mg/K...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：BACKGROUND:Human DNA topoisomerase I (topo 1) is an essential nuclear enzyme involved in vital cellular processes and the sole target of antitumor drugs of the camptothecin (CPT) family. The CPT derivative topotecan (Tpt, Hycamtin) is currently used in clinic, its effectiveness varying considerably for different types ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-05-01 00:00:00
abstract：:Experiments were designed to compare the efficacy of recombinant IL-2 immunotherapy and IL-2 gene therapy of i.p. growing murine plasmacytoma X63-Ag8.653. The kinetics of peritoneal exudate mononuclear cells were monitored during the progression and gene therapy of the plasmacytoma, using cytofluorometric analysis and...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：:A pilot study to evaluate the predictive value of Indium-111-labelled somatostatin analogue [DTPA-D-Phe1]-octreotide scintigraphy (OctreoScan111) in the Octreotide treatment of hormone-refractory prostatic adenocarcinoma was initiated. Ten patients were investigated with OctreoScan111 with regard to disease extension ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：BACKGROUND:Several attempts have been made to determine whether interphase fluorescence in situ hybridization (I-FISH) on bone marrow or peripheral blood specimens is a good alternative to conventional cytogenetics (CC) in calculating the residual proportion of Philadelphia (Ph) chromosome-positive cells during treatme...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-07-01 00:00:00
abstract：BACKGROUND:Recent studies have demonstrated that heparin-binding epidermal growth factor-like growth factor (HB-EGF) plays a role in carcinogenesis and carcinoma progression. In this study we investigated the expression of HB-EGF in human colonic non-neoplastic and neoplastic tissues. MATERIALS AND METHODS:We performe...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
abstract：:We have established a practical system for generating antitumor effector lymphocytes using the tumor antigen peptide CEA and cultured dendritic cells (DCs), and have also characterized effector cells. DCs were induced from the adherent cell population of autologous peripheral blood mononuclear cells (PBMCs) obtained f...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
abstract：BACKGROUND/AIM:Orbital metastasis of systemic cancer is exceedingly rare. This is a case report of a patient treated for locally recurrent vulvar melanoma who later presented with unilateral proptosis and was found to have an isolated biopsy-proven extraocular muscle metastasis. PATIENTS AND METHODS:A 94-year-old fema...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：10.21873/anticanres.12658
更新日期：2018-06-01 00:00:00
abstract：:The aim of this study was to investigate the relationship between Technetium-99m (Tc-99m) tetrofosmin accumulation in tumors which may represent the expression of multidrug resistance (MDR)--mediated P-glycoprotein (Pgp) and multidrug resistance-associated protein (MRP) as well as response to chemotherapy in patients ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：:Tumor angiogenesis seems to be an important factor in determining therapeutic effectiveness and patient prognosis. An abundance of data on tumor angiogenesis is available and it clearly shows that most human solid tumors are heterogeneously vascularized and oxygenated and contain hypoxic regions. Such regions and area...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2001-11-01 00:00:00
abstract：BACKGROUND:Locally recurrent rate of advanced head and neck squamous cell carcinoma (HNSCC) still remains high and the treatment is controversial. PATIENTS AND METHODS:We retrospectively analyzed ninety-three patients with recurrent advanced oral cancer from 2009 to 2013. Sixty-four patients are in the docetaxel with ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-07-01 00:00:00
abstract：BACKGROUND:It has been suggested that prognosis in familial breast cancer could be influenced by the parent of origin, with a worse prognosis when inherited paternally. This study aimed to investigate the effect of the parent of origin on prognosis. PATIENTS AND METHODS:Index patients were divided into two study group...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11442
更新日期：2017-03-01 00:00:00
abstract：BACKGROUND/AIM:γδ T cells mediate cytotoxicity against prostate cancer (PCa) cells in vitro; however, the clinical efficacy of γδ T cell-targeted immunotherapy for recurrent and metastatic PCa is unsatisfactory. We hypothesized that the resistance of recurrent and metastatic PCa to γδ T cells is related to the presence...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14559
更新日期：2020-10-01 00:00:00
abstract：UNLABELLED:The aim of this study was to examine the interobserver variance in delineating the contour of unresectable pancreatic cancer for chemoradiotherapy. PATIENTS AND METHODS:CT images of two cases of unresectable pancreatic tumors (head and body cancer) were sent to eight radiation therapy facilities in a CD-ROM...
journal_title：Anticancer research
pub_type： 杂志文章,多中心研究
doi：
更新日期：2007-07-01 00:00:00
abstract：BACKGROUND/AIM:Interleukin-32 (IL32) has been newly-identified as a proinflammatory cytokine. In the present study, we aimed to clarify the clinical role of IL32-positive cells in esophageal squamous cell cancer (ESCC) and regulatory T-cell (Treg) infiltration in the stroma. A total of 179 patients with ESCC who underw...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2015-05-01 00:00:00
abstract：:In the present study the effects of the anticancer drug Doxorubicin (Dox) on necrosis development and cell lethality of multicellular DU-145 spheroids (MCS) were examined. Multicellular spheroids consist of a peripheral rim of proliferating cells, a inner shell of nonproliferating, quiescent cells and a central core o...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
abstract：:The tumour samples ot 23 patients (9 male, 14 female, aged 28-85) were randomly selected for the study. DNA was isolated from paraffin embedded tissue for quantitative dot-blot hybridization, in order to determine the amplification values for the c-myc and K-ras oncogenes. The clinical and histological parameters stud...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:We assessed the effect of granulocyte apheresis in patients exhibiting increased granulocyte-to-lymphocyte ratio in order to overcome granulocytosis occurring in the terminal stages of malignancies. 17 patients with post-operative recurrent metastatic tumors including 6 gastric, 3 colonic, 2 rectal, 1 esophageal and 5...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1995-05-01 00:00:00
abstract：BACKGROUND/AIM:4H-1-Benzopyran-4-ones (chromones) have provided backbone structure for the chemical synthesis of potent anticancer drugs. In this study, the cytotoxicity of fifteen 2-(N-cyclicamino)chromone derivatives was investigated and subjected to quantitative structure-activity relationship (QSAR) analysis. MATE...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12674
更新日期：2018-07-01 00:00:00
abstract：:Tumor-associated glycoprotein (TAG) 72 is a mucin-like protein of high molecular weight (220-400 kd). Elevated serum levels of TAG72 are preferentially observed in patients suffering from gastric cancer. Additionally the determination of TAG72 may be a helpful tool in the management of patients suffering from mucinous...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-11-01 00:00:00
abstract：:The human MAGE gene products are recognized by major histocompatibility complex-restricted cytotoxic T lymphocytes. We analyzed by RT-PCR the expression of MAGE-1, MAGE-2, MAGE-3 and HLA-A2 genes in 10 human gastric cancer cell lines and 46 human stomachs removed due to advanced gastric carcinomas. All the cell lines ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:The development of T-lymphocytes (T cells) constitute one of the basic and most vital processes in immunology. Conventional mathematical models, being based on the systems theory, fail to sufficiently distinguish the constituents of thymocytes and are thus of limited significance. On the basis of some well thought-out...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-05-01 00:00:00
abstract：:Novel treatment strategies for cancer that are based on a more detailed understanding over the tumor biology are based on the latest new technology and are expected to improve the current treatment outcome for patients with cancer. However, many of these strategies still have one common and critical problem, being the...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2014-08-01 00:00:00
abstract：:The experimental techniques, methods of evaluation, and environmental implications of the findings of chemical carcinogenesis studies and related subject areas are herein critically reviewed. It is concluded that firstly, certain presently used procedures provide the best possibilities for the study of the hazardous n...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1985-09-01 00:00:00
abstract：:Dendritic cells (DCs) are professional antigen presenting cells. Two x 10(7) peripheral blood mononuclear cells (PBMCs) from each of 6 melanoma patients and 4 normal donors were incubated in 6-well plates. The adherent cells were cultured in GM-CSF (800 U/ml) and IL-4 (1000 U/ml) for 10 days. Anti-CD80, anti-CD86, ant...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
abstract：BACKGROUND:Metastatic breast cancer remains a major clinical issue despite progress achieved in recent years. Three randomised trials have demonstrated the benefit of combining bevacizumab with various taxane schedules. Herein, this study sought to investigate an alternative bevacizumab-taxane regimen as first-line tre...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2010-07-01 00:00:00
abstract：BACKGROUND/AIM:In a previous study, we showed that amentoflavone promotes sorafenib-induced apoptosis in hepatocellular carcinoma (HCC) cells in vitro. However, whether amentoflavone augments anticancer efficacy of sorafenib in HCC in vivo is unknown. The aim of the present study was to verify the anticancer effect of ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12452
更新日期：2018-04-01 00:00:00
abstract：BACKGROUND/AIM:Despite numerous studies, the etiology of chronic lymphocytic leukemia (CLL) remains unknown. A hypothesis of autoantigen stimulation in leukemic clone selection might explain 'stereotypy' of B-cell receptors. In healthy cells, cofilin-1 (CFL1) has multiple functions. Its role was described in several ma...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14653
更新日期：2020-11-01 00:00:00
abstract：:We report the synthesis of a series of merocyanines and the structure-effect relationship as cytotoxic agents for human adenocarcinoma cells in vitro. The cytotoxicity of these compounds in vitro makes them potential anticancer agents. One of these compounds was found active on human and mouse tumor models. ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1987-11-01 00:00:00
