Abstract:
BACKGROUND/AIM:Oxysterol plays important physiological roles in diverse biological processes including apoptosis. However, the mechanisms underlying oxysterol-induced apoptosis remain unknown. 25-hydroxycholesterol (25-HC) is an oxysterol synthesized by cholesterol 25-hydroxylase from cholesterol during sterol metabolism. The aim of present study was to investigate 25-HC-induced apoptosis and associated signalling pathways in FaDu cells, which is originated form human head and neck squamous cell carcinoma cells. MATERIALS AND METHODS:25-HC-induced apoptosis was investigated by cell cytotoxicity assay using MTT, cell viability assay using cell LIVE/DEAD cell viability assay, haematoxylin & eosin staining, nuclear staining, fluorescence-activated cell sorting, western blotting using specific antibodies associated with extrinsic and intrinsic apoptosis pathways, and caspase-3/-7 activity assay in FaDu cells. RESULTS:25-HC dose-dependently decreased the viability of FaDu cells and up-regulated apoptotic events, such as alteration in morphology, and nuclear condensation. Flow cytometric analysis showed an increase in apoptotic population upon 25-HC treatment, suggesting that 25-HC induces apoptosis in FaDu cells. Moreover, 25-HC-induced apoptosis in FaDu cells was dependent on the activation of caspases by Fas antigen ligand-triggered death receptor-mediated extrinsic pathway and mitochondria-dependent intrinsic pathway via mitogen activated protein kinases. CONCLUSION:Cholesterol-derived oxysterol, 25-HC has potential anti-cancer function in FaDu cells and may have potential properties for the discovery of anti-cancer agents.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
You JS,Lim H,Kim TH,Oh JS,Lee GJ,Seo YS,Kim DK,Yu SK,Kim HJ,Kim CS,Kim JSdoi
10.21873/anticanres.14009subject
Has Abstractpub_date
2020-02-01 00:00:00pages
779-788issue
2eissn
0250-7005issn
1791-7530pii
40/2/779journal_volume
40pub_type
杂志文章abstract::Allelic loss at specific chromosomal loci was demonstrated in carcinoma of the cervix (CaCx) obtained from Hong Kong Chinese by restriction fragment length polymorphism analysis. Altogether 12 patients with CaCx, 7 with cervical intraepithelial neoplasia (CIN) and 7 with normal cervical tissues were studied. Polymorph...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1992-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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journal_title:Anticancer research
pub_type: 杂志文章,评审
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journal_title:Anticancer research
pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00
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journal_title:Anticancer research
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
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journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:1999-03-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-12-01 00:00:00
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journal_title:Anticancer research
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-08-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
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doi:
更新日期:2010-04-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-07-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
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更新日期:2001-11-01 00:00:00
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更新日期:2000-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-05-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
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更新日期:1998-11-01 00:00:00
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journal_title:Anticancer research
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doi:
更新日期:2003-05-01 00:00:00
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journal_title:Anticancer research
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doi:
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