Abstract：
BACKGROUND:The purpose of the study was to test the feasibility of laser scanning cytometry (LSC) to simultaneously measure estrogen receptor (ER) and nucleolin (NU) expression in the nuclei of the same individual breast cancer cells. MATERIALS AND METHODS:Cancer cells from 64 breast tumors were labeled with anti-NU and biotinylated anti-ER antibodies, then with secondary FITC-conjugated antibody and streptavidin-APC conjugate, respectively, and measured by LSC. The expression of NU in the nucleus and NU aggregates (NUA), number of NUA, nuclear and NUA areas and ER expression were assessed for aspiration each cell. RESULTS:ER-bound APC fluorescence correlated with nuclear NU (r=0.65; p<0.001) and NUA-bound FITC fluorescence (r=0.59; p<0.001). Good correlation was found between percentages of ER-positive cells in LSC and by image analysis in paraffin-embedded sections (r=0.59, p<0.001). CONCLUSION:ER and NU expression can be measured simultaneously in the same nuclei of breast cancer cells.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Masiuk M,Urasinska E,Domagala Wsubject
Has Abstractpub_date
2004-03-01 00:00:00pages
963-6issue
2Beissn
0250-7005issn
1791-7530journal_volume
24pub_type
杂志文章abstract：AIM:To examine patterns of clinical practice in locally advanced esophageal cancer among US radiation oncologists after publication of the CROSS trial. MATERIALS AND METHODS:US radiation oncologists were surveyed on 13 questions pertaining to the management of esophageal cancer. Respondents' demographics and their cli...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12996
更新日期：2018-11-01 00:00:00
abstract：BACKGROUND/AIM:Roles for mutant (mt) KRAS in the innate immune microenvironment in colorectal cancer (CRC) were explored. MATERIALS AND METHODS:Human CRC HCT116-derived, mtKRAS-disrupted (HKe3) cells that express exogenous mtKRAS and allogenic cytokine-activated killer (CAK) cells were co-cultured in 3D floating (3DF)...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14465
更新日期：2020-08-01 00:00:00
abstract：BACKGROUND:Basal cell carcinomas (BCCs) are the most common cutaneous tumors, accounting for approximately 70% of all malignant skin tumors. A morphometric analysis was performed to evaluate whether morphometric parameters of the nuclei of BCC tumor cells may serve as predictors for biological behavior like recurrence ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：BACKGROUND:Pemetrexed-induced eyelid edema is a rare side-effect of pemetrexed treatment. PATIENTS AND METHODS:Retrospective analysis of the incidence and severity of eyelid edema was conducted in patients treated with pemetrexed in a single institution. RESULTS:Eighty-six patients received pemetrexed-containing chem...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
abstract：BACKGROUND:Dihydroceramide desaturase 1 (DES) is the enzyme responsible for converting dihydroceramide into ceramide in the de novo sphingolipid biosynthesis pathway. Dihydroceramide can inhibit ceramide channel formation to interfere with apoptosis. We have shown that following ceramide synthase knockdown, photodynami...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：BACKGROUND:Ano-genital carcinoma is a polyfactorial and polygenic disease. Certain strains of human papillomavirus (HPV) have been detected in a high percentage of patients. It has been suggested that p53 polymorphisms may be relevant for the interaction with viral proteins that inactivates p53. MATERIALS AND METHODS:...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-07-01 00:00:00
abstract：:Keratinocytes originating from adult human hair follicles, the most convenient biopsy tissue, can be serially cultured using a combination of two techniques. Primary cultures are established using plucked scalp hair follicles and the bovine eye lens capsule as a growth substrate. Subsequently, cells from these culture...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:MDL 103,323, an enclomiphene analog, was tested for binding to the estrogen receptor, inhibition of human tumor xenografts and prevention of carcinogen-induced mammary tumors. MDL 103,323 had 5-6 fold greater affinity for the human estrogen receptor than did either tamoxifen or enclomiphene. Consistent with enhanced b...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：BACKGROUND:Although in vitro and clinical studies indicate that overexpression of P-glycoprotein (Pgp), p53, or metallothionein (MT) is involved in modulating drug resistance/sensitivity of cancer cells, the clinical relevance of the overexpression remains to be elucidated. MATERIALS AND METHODS:In this paper the expr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：BACKGROUND:Different phenomena can result in enlargement of mental foramen and mandibular canal. At the foreground of diagnosis is the assessment of the biological properties of the tissue which causes such detailed lesions of the skeleton. CASE REPORT:This report describes a palpable mass at the site of the mental fo...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：10.21873/anticanres.13554
更新日期：2019-08-01 00:00:00
abstract：BACKGROUND:Adverse outcomes in breast cancer patients treated with recombinant human erythropoietin (rhEpo) have been linked to the expression of Epo-receptors (EpoR) in cancer cells, although limited data on the clinical significance of these observations are available. PATIENTS AND METHODS:Tissue samples from 107 pa...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
abstract：BACKGROUND:The effectiveness of postoperative adjuvant chemotherapy for surgically staged grade 3 endometrial cancer was investigated. PATIENTS AND METHODS:Sixty-three consecutive patients with grade 3 endometrial cancer (49 with surgical stage I-II disease and 14 with stage III disease) were treated surgically. Posto...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-05-01 00:00:00
abstract：:Initial events in the metastatic spread of tumours involve loss of cell-cell adhesion within the primary tumour mass. The integrity and morphology of epithelial tumour cell colonies is maintained primarily by cell-cell adhesions mediated by E-cadherin and its associated intracellular catenin molecules. Hepatocyte grow...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:The aim of the study was the evaluation of the detectability of treated liver metastases using one FDG-PET measurement. The study includes 42 patients (80 lesions) from different primary tumours. Standardized Uptake Values (SUV) as well as the tumour to liver Ratio (T/L) were used for evaluation. A T/L > 1.0 was consi...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：BACKGROUND:Treatment for advanced pancreatic cancer is still very unsatisfactory. Treosulfan is an alkylating agent used for conventional, as well as high-dose chemotherapy regimens, whereby plasma concentrations over 500 μg/ml can be achieved. We investigated the effects of treosulfan on pancreatic cancer cell lines. ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-04-01 00:00:00
abstract：:Two different techniques [reduction of a tetrazolium derivative (MTT) and 3HTdR uptake assays] were compared in order to evaluate the cytotoxic effects of different chemotherapeutic agents in vitro. The cytotoxicities of Melphalan, hexamethylmelamine and seven derivatives, and daunorubicin were measured on P388D1 mous...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:The active vitamin D3, 1alpha,25-dihydroxyvitamin D3 (1,25(OH)2D3), is now well recognized as a potent regulator of cell proliferation and differentiation in addition to possessing a regulatory effect on calcium and phosphorus metabolism. From research on the synthesis of 1,25(OH)2D3 analogs with the goal of separatin...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-09-01 00:00:00
abstract：BACKGROUND:Both liposomal doxorubicin (LD) and docetaxel (D) have a broad range of activity against solid tumors, including advanced pancreatic cancer (APC), as single agents, while their combination has produced encouraging response rates in the treatment of several malignancies. We have conducted a Phase-II study in ...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：:Therapy of patients with metastatic renal cell carcinoma (mRCC) requires sequential use of several agents with different mechanisms and minimal cross-resistance between the different agents. Tyrosine kinase inhibitors (TKIs) and mammalian target of rapamycin (mTOR) inhibitors prolong progression-free survival (PFS) in...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-10-01 00:00:00
abstract：BACKGROUND/AIM:Bladder cancer (BLCA, urothelial bladder cancer) is one of the most common malignancies with increasing incidence and mortality worldwide. Poor diagnosis and the limitation of treatment is still an unmet need in clinical practice. γδ T-Cells have been paid increasing attention because of their potent cyt...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.14525
更新日期：2020-09-01 00:00:00
abstract：:The single and combined antitumor effects of adriamycin and a major histocompatibility complex non-restricted human cytotoxic T cell line (TALL-104) were evaluated in severe combined immunodeficient (SCID) mice engrafted subcutaneously with a biopsy sample from a patient with a highly metastatic gastric carcinoma. Che...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
abstract：BACKGROUND:Irofulven (MGI 114) is a novel, clinically active sesquiterpene whose mechanism of action is not fully understood. We sought to identify apoptotic effectors induced by this agent in human pancreatic cancer cells. MATERIALS AND METHODS:MTT assay was used to assess IC50-Apoptosis was quantitated by flow cytom...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-05-01 00:00:00
abstract：BACKGROUND AND AIM:The Seventh American Joint Committee on Cancer (AJCC)/TNM classification defined that the classification for adenocarcinoma of the esophagogastric junction (AEG) was included in the esophageal category. However, there still remain various classifications in AEG such as the Seventh AJCC/TNM of the eso...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-08-01 00:00:00
abstract：:In a prospective study comprising 447 women with endometrial carcinoma stages I-II, the prognostic significance of clinical and flow cytometric variables was evaluated in univariate and multivariate analyses. The parameters studied included age, uterine cavity depth, clinical stage, histopathologic grade, myometrial i...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
abstract：:Recent results concerning new antitumor metallocene derivatives have shown that not only numerous titanocene dihalides and bis(pseudohalides) exhibit antiproliferative activity, but that also some titanocene complexes (C5H5)2TiX2 containing carboxylates or hydrochlorinated aminothiophenolates as ligands X, are charact...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：BACKGROUND:Tumour stage and the appropriate course of treatment in patients with breast cancer are primarily characterized by the state of metastasis in the axillary lymph nodes. In recent years, substantial research has focused on the prediction of lymph node status based on various pathological and molecular markers ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-09-01 00:00:00
abstract：:Calcitriol (1,25-dihydroxyvitamin D3), the active form of vitamin D, promotes growth inhibition and differentiation in prostate cancer (PCa) cells. To unravel the molecular pathways of calcitriol actions, cDNA microarray analysis was used to identify novel calcitriol target genes including two that play key roles in t...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2006-07-01 00:00:00
abstract：:Gross cystic disease (GCD) represents an advanced form of fibrocystic disease of the breast. Bearers of macrocysts have been reported to be at risk of developing breast cancer. Natural killer (NK) cells are a lymphocyte subset deeply involved in immunosurveillance against neoplasia. We investigated whether breast cyst...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:p73, a structural homologue of the tumor suppressor gene, p53, has recently been identified and mapped to chromosome 1p36, where genomic loss of heterozygosity (LOH) often occurs in human hepatocellular carcinoma (HCC). To determine whether p73 is involved in the development of HCC and whether there is an inverse corr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
abstract：:A series of simple alpha, beta-unsaturated carbonyl compounds (1-26) was characterized for their cytotoxic profiles against oral human normal and tumor cells. Several cycloalkenones showed potent cytotoxic activities against human oral squamous cell carcinoma HSC-2 cell line. Among them, 4,4-dimethyl-2-cyclopenten-1-o...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
