An enzyme-immunoassay for myoglobin in human serum and urine. Method development, normal values and application to acute myocardial infarction.

Abstract:

:An enzyme-immunoassay has been developed for the detection of myoglobin in human serum and urine which is specific, accurate, precise, and has a sensitivity of 3 ng/ml. When compared with radioimmunoassay, the enzyme-immunoassay gives markedly similar results. Sera from normal adults had a myoglobin concentration in the range 3-65 ng/ml, and 64% of the same group had detectable myoglobinuria (range 3-11.5 ng/ml). All of 8 patients with definite acute myocardial infarction had raised serum myoglobin levels (range 200-1125 ng/ml) either at admission or 4 h later. Myoglobin concentration returned to normal in 6 patients, and in the remaining 2 patients there was evidence of infarct extension. Urinary myoglobin excretion was variable. One patient with possible acute myocardial infarction had elevated serum myoglobin (413 ng/ml 4 h post admission) and 5 patients with no evidence of infarction had normal levels (15-53 ng/ml). The results suggest that detection of serum myoglobin by enzyme-immunoassay may be a valuable test in the early diagnosis of acute myocardial infarction.

journal_name

Pathology

journal_title

Pathology

authors

Cloonan MJ,Bishop GA,Wilton-Smith PD,Carter IW,Allan RM,Wilcken DE

doi

10.3109/00313027909059049

subject

Has Abstract

pub_date

1979-10-01 00:00:00

pages

689-99

issue

4

eissn

0031-3025

issn

1465-3931

journal_volume

11

pub_type

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