Abstract:
:The epithelium lining the epididymis in the male reproductive tract maintains a luminal environment that promotes sperm cell maturation. This process is dependent on the coordinated expression of many genes that encode proteins with a role in epithelial transport. We previously generated genome-wide maps of open chromatin in primary human epididymis epithelial (HEE) cells to identify potential regulatory elements controlling coordinated gene expression in the epididymis epithelium. Subsequent in silico analysis identified transcription factor-binding sites (TFBS) that were over-represented in the HEE open chromatin, including the motif for paired box 2 (PAX2). PAX2 is a critical transcriptional regulator of urogenital tract development, which has been well studied in the kidney but is unexplored in the epididymis. Due to the limited lifespan of primary HEE cells in culture, we investigated the role of PAX2 in an immortalized HEE cell line (REP). First, REP cells were evaluated by DNase I digestion followed by high-throughput sequencing and the PAX2-binding motif was again identified as an over-represented TFBS within intergenic open chromatin, though on fewer chromosomes than in the primary HEE cells. To identify PAX2-target genes in REP cells, RNA-seq analysis was performed after siRNA-mediated depletion of PAX2 and compared with that with a non-targeting siRNA. In response to PAX2-repression, 3135 transcripts were differentially expressed (1333 up-regulated and 1802 down-regulated). Novel PAX2 targets included multiple genes encoding proteins with predicted functions in the epididymis epithelium.
journal_name
Mol Hum Reprodjournal_title
Molecular human reproductionauthors
Browne JA,Yang R,Song L,Crawford GE,Leir SH,Harris Adoi
10.1093/molehr/gau075subject
Has Abstractpub_date
2014-12-01 00:00:00pages
1198-207issue
12eissn
1360-9947issn
1460-2407pii
gau075journal_volume
20pub_type
杂志文章abstract::Human chromosome deletions in Yq11 seem to occur frequently as de novo mutation events in men with idiopathic azoospermia or severe oligozoospermia. However, the molecular extensions of these deletions are variable. They can be large and therefore visible under the microscope or small, not visible under the microscope...
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更新日期:2014-09-01 00:00:00
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2010-02-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章,评审
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更新日期:2020-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2019-11-30 00:00:00
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pub_type: 杂志文章
doi:10.1093/molehr/3.5.445
更新日期:1997-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/molehr/5.11.995
更新日期:1999-11-01 00:00:00
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更新日期:2002-06-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:1997-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/molehr/gax041
更新日期:2017-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-07-01 00:00:00
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更新日期:2007-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-12-01 00:00:00
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更新日期:2001-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-11-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2013-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-08-01 00:00:00
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更新日期:1999-08-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2003-08-01 00:00:00
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更新日期:1999-01-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2017-12-01 00:00:00