Abstract:
:The human endometrium is a dynamic tissue, which undergoes extensive tissue remodelling during the menstrual cycle. Due to their involvement in such processes, several well-characterized matrix metalloproteinases (MMP) have previously been studied in the endometrium. MMP-26 is a newly described matrilysin. We studied MMP-26 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Tissue distribution and cycle variation was examined using in-situ hybridization, Northern blot analyis and real time PCR. The probes for Northern blot analysis and real time PCR recognized non-overlapping sequences. MMP-26 was localized exclusively in epithelial cells of both glands and the luminal surface. Expression increased during the proliferative phase to a maximum at mid-cycle, then decreased to non-detectable levels in the late secretory and menstrual phases. Expression of MMP-26 mRNA in endometrial tissue explants in vitro required stimulation with both estradiol and progesterone. The tissue content of c-jun mRNA was assayed, since c-jun, as part of the enhancer complex AP-1, may be involved in regulation of MMP-26 gene transcription. The pattern of c-jun expression over the menstrual cycle was similar to that of MMP-26. Epithelial expression in the peri- and post-ovulatory stages of the menstrual cycle suggests the involvement of MMP-26 in reproductive processes.
journal_name
Mol Hum Reprodjournal_title
Molecular human reproductionauthors
Pilka R,Whatling C,Domanski H,Hansson S,Eriksson P,Casslén Bdoi
10.1093/molehr/gag039subject
Has Abstractpub_date
2003-05-01 00:00:00pages
271-7issue
5eissn
1360-9947issn
1460-2407journal_volume
9pub_type
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journal_title:Molecular human reproduction
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journal_title:Molecular human reproduction
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journal_title:Molecular human reproduction
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gau059
更新日期:2014-10-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/7.7.665
更新日期:2001-07-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gal054
更新日期:2006-07-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gau075
更新日期:2014-12-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gal094
更新日期:2007-01-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/5.8.732
更新日期:1999-08-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gah092
更新日期:2004-09-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/8.5.434
更新日期:2002-05-01 00:00:00
abstract::After meiosis, during the final stages of spermatogenesis, the haploid male genome undergoes major structural changes, resulting in a shift from a nucleosome-based genome organization to the sperm-specific, highly compacted nucleoprotamine structure. Recent data support the idea that region-specific programming of the...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gar063
更新日期:2012-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:1997-09-01 00:00:00
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更新日期:1996-11-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2017-06-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:1996-07-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2014-02-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章
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更新日期:2016-04-01 00:00:00
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更新日期:1996-01-01 00:00:00
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更新日期:1999-05-01 00:00:00
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更新日期:2019-04-01 00:00:00