Immortalization of normal human cytotrophoblast cells by reconstitution of telomeric reverse transcriptase activity.

abstract：:We have previously reported that endometrial expression of matrix metalloproteinase (MMP)-26 mRNA comes to a maximum in the early secretory phase. Since tissue inhibitor of metalloproteinase (TIMP)-4 is a potent inhibitor of MMP-26, the objective of this study was to identify the pattern of TIMP-4 mRNA expression in t...

journal_title：Molecular human reproduction

authors： Pilka R,Domanski H,Hansson S,Eriksson P,Casslén B

更新日期：2004-09-01 00:00:00

abstract：:An understanding of the relationship between nuclear morphology and DNA function is important in cytology and preimplantation diagnosis. In this study, direct polymerase chain reaction (PCR) amplification was used to diagnose the common delta F508 mutation of cystic fibrosis in 62 biopsied human embryo cells. The nucl...

journal_title：Molecular human reproduction

authors： Cui KH,Matthews CD

更新日期：1996-01-01 00:00:00

abstract：:During pregnancy, a cervical connective tissue remodelling takes place, clinically recognized as softening, effacement and dilatation. The role of oestrogens and their receptors (ER) in this process is not clear. ERalpha is a ligand-activated transcription factor involved in many physiological processes. The identific...

journal_title：Molecular human reproduction

authors： Wang H,Stjernholm Y,Ekman G,Eriksson H,Sahlin L

更新日期：2001-03-01 00:00:00

abstract：:Charcot-Marie-Tooth (CMT) disease is the 'common' name for a range of hereditary peripheral neuropathies. CMT1 is the most common form and is transmitted in an autosomal dominant manner. CMT1A maps to chromosome 17p11.2 and is caused, in the majority of cases, by a 1.5 Mb DNA duplication, that includes the peripheral ...

journal_title：Molecular human reproduction

authors： De Vos A,Sermon K,De Rijcke M,Goossens V,Henderix P,Van Ranst N,Platteau P,Lissens W,Devroey P,Van Steirteghem A,Liebaers I

更新日期：2003-07-01 00:00:00

abstract：:Oligozoospermia (low sperm count) is a common semen deficiency. However, to date, few genetic defects have been identified to cause this condition. Moreover, even fewer molecular genetic diagnostic tests are available for patients with oligozoospermia in the andrology clinic. Based on animal and gene expression studie...

journal_title：Molecular human reproduction

authors： Yatsenko AN,Georgiadis AP,Murthy LJ,Lamb DJ,Matzuk MM

更新日期：2013-06-01 00:00:00

abstract：:Folate deficiency has been linked to a wide range of pregnancy disorders. Most research about folate-deficiency has focused on the embryo itself, little attention has been paid to possible effects on the placenta. According to our results, the morphology of the placenta, endocrine function, and the expression of genes...

journal_title：Molecular human reproduction

authors： Yin X,Gao R,Geng Y,Chen X,Liu X,Mu X,Ding Y,Wang Y,He J

更新日期：2019-06-06 00:00:00

abstract：:Oocyte quality is one of the most important aspects of in vitro embryo development. Extensive epigenetic programming must occur during oocyte growth and maturation. A specific DNA methylation pattern of the imprinted genes must be established on differentially methylated regions (DMR). The insulin-like growth factor 2...

journal_title：Molecular human reproduction

authors： Fagundes NS,Michalczechen-Lacerda VA,Caixeta ES,Machado GM,Rodrigues FC,Melo EO,Dode MA,Franco MM

更新日期：2011-02-01 00:00:00

abstract：:The epithelium lining the epididymis in the male reproductive tract maintains a luminal environment that promotes sperm cell maturation. This process is dependent on the coordinated expression of many genes that encode proteins with a role in epithelial transport. We previously generated genome-wide maps of open chrom...

journal_title：Molecular human reproduction

authors： Browne JA,Yang R,Song L,Crawford GE,Leir SH,Harris A

更新日期：2014-12-01 00:00:00

abstract：:Fatty acid amide hydrolase (FAAH) is involved in embryo development and implantation. Sex hormones down-modulate FAAH activity in the mouse uterus. However, the regulation of the FAAH gene in the uterus is unknown. Our results showed that FAAH mRNA is localized to uterine epithelial cells and circular myometrium durin...

journal_title：Molecular human reproduction

authors： Xiao AZ,Zhao YG,Duan EK

更新日期：2002-07-01 00:00:00

abstract：:Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency is the most common defect in fatty acid oxidation. The disease is inherited in an autosomal recessive fashion (carrier frequency around 1 in 70) and probably affects as many as 1 in 10000 new-borns. Affected children usually present within the two first years of li...

journal_title：Molecular human reproduction

authors： Sermon K,Henderix P,Lissens W,De Vos A,Vandervorst M,Vanderfaeillie A,Vamos E,Van Steirteghem A,Liebaers I

更新日期：2000-12-01 00:00:00

abstract：:The two estrogen receptors, ERalpha and ERbeta, are likely to have roles in the pathophysiology of fibroid development. They have been detected in myometrial and leiomyoma (fibroid) tissue, but the cell types expressing ERalpha and ERbeta have not been determined. ERs have also been detected in human endothelial cells...

journal_title：Molecular human reproduction

authors： Gargett CE,Bucak K,Zaitseva M,Chu S,Taylor N,Fuller PJ,Rogers PA

更新日期：2002-08-01 00:00:00

abstract：:Mitotic centromere-associated kinesin (MCAK) is an ATP-dependent microtubule (MT) depolymerase regulated by Aurora kinase (AURK) phosphorylation and implicated in resolution of improper MT attachments in mitosis. Distribution of MCAK was studied in oocyte maturation by anti-MCAK antibody, anti-tubulin antibody, anti-A...

journal_title：Molecular human reproduction

authors： Vogt E,Sanhaji M,Klein W,Seidel T,Wordeman L,Eichenlaub-Ritter U

更新日期：2010-09-01 00:00:00

abstract：STUDY QUESTION:Which different pathways and functions are altered in rhesus monkey oocytes that fail to mature after an ovulatory stimulus? SUMMARY ANSWER:Failed to mature (FTM) oocytes complete a large portion of the transition in transcriptome composition associated with normal maturation, but also manifest numerous...

journal_title：Molecular human reproduction

authors： Ruebel ML,Schall PZ,Midic U,Vincent KA,Goheen B,VandeVoort CA,Latham KE

更新日期：2018-10-01 00:00:00

abstract：:Maturation (M-phase) promoting factor (MPF) plays a pivotal role in oocytes during their maturation. This review concentrates on its function at three important time-points. First, its activation during meiotic progression from prophase I arrest at germinal vesicle breakdown. Second, its role during the transition fro...

journal_title：Molecular human reproduction

authors： Jones KT

更新日期：2004-01-01 00:00:00

abstract：:Interleukin (IL)-33, a newly described member of the IL-1 family, has been reported to facilitate primary tumor progression and metastatic dissemination. However, its biological function on decidual stromal cells (DSCs) remains unclear. In this study, we tested the hypothesis whether IL-33 promotes proliferation and i...

journal_title：Molecular human reproduction

authors： Hu WT,Li MQ,Liu W,Jin LP,Li DJ,Zhu XY

更新日期：2014-04-01 00:00:00

abstract：:The molecular mechanism of stress-associated reproductive dysfunction is complex and largely unknown. This study was designed to systematically analyze molecular effects of systemic in vivo blockade of α1-adrenergic receptors (α1-ADRs) on stress-induced disturbance of cAMP/cGMP signaling in testosterone-producing Leyd...

journal_title：Molecular human reproduction

authors： Stojkov NJ,Baburski AZ,Bjelic MM,Sokanovic SJ,Mihajlovic AI,Drljaca DM,Janjic MM,Kostic TS,Andric SA

更新日期：2014-01-01 00:00:00

abstract：:Etoposide is widely used in the treatment of patients with testicular cancer. The mechanism underlying apoptosis induction in cancer cells has been studied in different cell types, but it is not known whether the same factors participate in viable germ cells undergoing programmed cell death. Since testicular cancer pr...

journal_title：Molecular human reproduction

authors： Ortiz RJ,Lizama C,Codelia VA,Moreno RD

更新日期：2009-06-01 00:00:00

abstract：:Mouse cauda epididymis were in-vivo transfected using the lipid FuGENE 6 as gene vector. Two gene constructions were employed: the p-GeneGRIP which codifies for the Green Fluorescent Protein (GFP) and the pSEAP-control that expresses an alkaline phosphatase as a secretion. Transfection was detected by fluorescence and...

journal_title：Molecular human reproduction

authors： Esponda P,Carballada R

更新日期：2009-06-01 00:00:00

abstract：:A rapid fluorescence in-situ hybridization (FISH) technique was used for direct chromosomal analysis on germ cells from an infertile male with large-headed spermatozoa. The interphase chromosomes were fluorescently-labelled using an extremely bright cyanine dye during a 5-15 min FISH procedure. Germ cells were analyse...

journal_title：Molecular human reproduction

authors： Yurov YB,Saias MJ,Vorsanova SG,Erny R,Soloviev IV,Sharonin VO,Guichaoua MR,Luciani JM

更新日期：1996-09-01 00:00:00

abstract：:Tumour necrosis factor-alpha (TNF-alpha is a pleiotropic cytokine synthesized throughout the female reproductive tract. Even though evidence has accumulated that supports its role in autocrine and paracrine processes, its expression and function in the human endometrium are still not completely understood. To gain a b...

journal_title：Molecular human reproduction

authors： von Wolff M,Classen-Linke I,Heid D,Krusche CA,Beier-Hellwig K,Karl C,Beier HM

更新日期：1999-02-01 00:00:00

abstract：:This study was undertaken to evaluate the effects of gamma-aminobutyric acid (GABA) and GABAergic agonists and antagonists on sperm kinematic parameters and hyperactivation, evaluated by a computer-assisted semen analysis (CASA) system, and intracellular cAMP content in 22 normozoospermic semen samples. Because of the...

journal_title：Molecular human reproduction

authors： Calogero AE,Hall J,Fishel S,Green S,Hunter A,D'Agata R

更新日期：1996-10-01 00:00:00

abstract：:Morphological assessments are the main way in which fertility clinics select in vitro generated embryo(s) for transfer to the uterus. However, it is widely acknowledged that the microscopic appearance of an embryo is only weakly correlated with its viability. Furthermore, the extent to which morphology is affected by ...

journal_title：Molecular human reproduction

authors： Fragouli E,Alfarawati S,Spath K,Wells D

更新日期：2014-02-01 00:00:00

abstract：:Polycystic ovary syndrome (PCOS) is a heterogeneous condition with unknown etiology and is considered to be the most common endocrine disorder in women of reproductive age. Two meta-analyses are presented here concerning the association of Plasminogen Activator Inhibitor 1 (PAI-1) 4G/5G polymorphism and the methylene-...

journal_title：Molecular human reproduction

authors： Bagos PG

更新日期：2009-01-01 00:00:00

abstract：:Human pregnancy represents a situation of semiallograft to maternal host. Therefore, it has been reported that tolerance to the fetal allograft represents a mechanism for maintaining a pregnancy. CD4(+)CD25(bright) regulatory T cells are known to play an important role in the development and maintenance of tolerance i...

journal_title：Molecular human reproduction

authors： Sasaki Y,Sakai M,Miyazaki S,Higuma S,Shiozaki A,Saito S

更新日期：2004-05-01 00:00:00

abstract：:Mammalian sperm undergo a series of maturation steps before acquiring fertilization competence. Our previous work demonstrated the importance of binder of sperm (BSP) proteins in bovine sperm capacitation. Recent studies identified a BSP-homologous DNA sequence in the human genome (BSPH1) and mRNA expression in the ep...

journal_title：Molecular human reproduction

authors： Lefebvre J,Boileau G,Manjunath P

更新日期：2009-02-01 00:00:00

abstract：:This study assesses the role of progesterone receptor membrane component 1 (PGRMC1) in actions of progesterone (P4) on human myometrium during pregnancy and labour. Myometrial tissues were obtained from non-pregnant patients during hysterectomy or pregnant women undergoing C-section at term and preterm, before and dur...

journal_title：Molecular human reproduction

authors： Wu W,Shi SQ,Huang HJ,Balducci J,Garfield RE

更新日期：2011-04-01 00:00:00

abstract：:Nuclear oligomerization domains (NODs) are cytosolic pattern recognition receptors (PRRs), present in epithelial cells, monocytes and dendritic cells. This study details their expression, regulation and role in human endometrium. Real-time PCR showed that NOD1 mRNA is constitutively expressed in endometrium. NOD2 is u...

journal_title：Molecular human reproduction

authors： King AE,Horne AW,Hombach-Klonisch S,Mason JI,Critchley HO

更新日期：2009-05-01 00:00:00

abstract：:The etiology and pathogenesis of endometriosis are complex with both genetic and environmental factors contributing to disease risk. Genome-wide association studies (GWAS) have identified multiple signals in the estrogen receptor 1 (ESR1) region associated with endometriosis and other reproductive traits and diseases....

journal_title：Molecular human reproduction

authors： Marla S,Mortlock S,Houshdaran S,Fung J,McKinnon B,Holdsworth-Carson SJ,Girling JE,Rogers PAW,Giudice LC,Montgomery GW

更新日期：2021-01-22 00:00:00

abstract：:Nitric oxide (NO) is synthesized from L-arginine by a family of enzymes known as the nitric oxide synthases (NOS). We have recently shown a NOS similar to constitutive brain NOS (bNOS) and endothelial NOS (ecNOS) to be present in spermatozoa. The aim of this study is to investigate NO production by human spermatozoa a...

journal_title：Molecular human reproduction

authors： Donnelly ET,Lewis SE,Thompson W,Chakravarthy U

更新日期：1997-09-01 00:00:00

abstract：STUDY QUESTION:Can enzymatically dispersed testicular cells from adult men reassemble into seminiferous cord-like structures in vitro? SUMMARY ANSWER:Adult human testicular somatic cells reassembled into testicular cord-like structures via dynamic interactions of Sertoli and peritubular cells. WHAT IS KNOWN ALREADY:I...

journal_title：Molecular human reproduction

authors： Mincheva M,Sandhowe-Klaverkamp R,Wistuba J,Redmann K,Stukenborg JB,Kliesch S,Schlatt S

更新日期：2018-02-01 00:00:00