Detection of mRNA transcripts of cyclic nucleotide phosphodiesterase subtypes in ejaculated human spermatozoa.

abstract：:Mammalian sperm undergo a series of maturation steps before acquiring fertilization competence. Our previous work demonstrated the importance of binder of sperm (BSP) proteins in bovine sperm capacitation. Recent studies identified a BSP-homologous DNA sequence in the human genome (BSPH1) and mRNA expression in the ep...

journal_title：Molecular human reproduction

authors： Lefebvre J,Boileau G,Manjunath P

更新日期：2009-02-01 00:00:00

abstract：:In recent years, the impact of sperm DNA damage on fertility has become an important issue. The different technologies developed to check sperm DNA fragmentation lead to the same conclusion: DNA damage negatively impacts upon reproductive processes. Oocyte DNA repair capacity is one of the cues to understanding embryo...

journal_title：Molecular human reproduction

authors： El-Mouatassim S,Bilotto S,Russo GL,Tosti E,Menezo Y

更新日期：2007-08-01 00:00:00

abstract：STUDY QUESTION:Can RNA sequencing of human cumulus cells (CC) reveal molecular pathways involved in the physiology of reproductive aging? STUDY FINDING:Senescent but not young CC activate gene pathways associated with hypoxia and oxidative stress. WHAT IS KNOWN ALREADY:Shifts in socioeconomic norms are resulting in l...

journal_title：Molecular human reproduction

authors： Molinari E,Bar H,Pyle AM,Patrizio P

更新日期：2016-08-01 00:00:00

abstract：:Follicular development and ovulation are major processes in the reproductive system. Understanding their complexity is important to female fertility treatments and the control of reproductive processes. Wnt signaling pathway components were shown to be involved in reproduction in animal models. The secreted frizzled-r...

journal_title：Molecular human reproduction

authors： Maman E,Yung Y,Cohen B,Konopnicki S,Dal Canto M,Fadini R,Kanety H,Kedem A,Dor J,Hourvitz A

更新日期：2011-07-01 00:00:00

abstract：:During early gestation, a considerable increase in different leukocyte subsets can be observed in the decidualized endometrium concomitantly to the invasion of cytotrophoblast cells (CTB). To date, it is still in question which factors induce this accumulation of immune cells and whether it is evoked by an in situ pro...

journal_title：Molecular human reproduction

authors： Segerer SE,Martignoni F,Bogdan A,Müller N,Kapp M,Dietl J,Rieger L,Kämmerer U

更新日期：2013-06-01 00:00:00

abstract：:Before the advent of intracytoplasmic sperm injection (ICSI) semen preparation techniques focused on the need to sustain the fertilizing potential of the spermatozoa particularly by reducing oxidative stress. However, for severely oligozoospermic patients treated by ICSI, sperm preparation protocols are used which aim...

journal_title：Molecular human reproduction

authors： Twigg J,Irvine DS,Houston P,Fulton N,Michael L,Aitken RJ

更新日期：1998-05-01 00:00:00

abstract：:No phenotypic effect is observed in most inversion heterozygotes. However, reproductive risks may occur in the form of infertility, spontaneous abortions or chromosomally unbalanced children as a consequence of meiotic recombination between inverted and non-inverted chromosomes. An odd number of crossovers within the ...

journal_title：Molecular human reproduction

authors： Chantot-Bastaraud S,Ravel C,Berthaut I,McElreavey K,Bouchard P,Mandelbaum J,Siffroi JP

更新日期：2007-01-01 00:00:00

abstract：:A molecule isolated from the peritoneal fluids of women undergoing laparoscopy for in-vitro fertilization techniques has been chemically characterized and identified as 1-palmitic-3-phosphorylcholine (lysophosphatidylcholine, LPC). This lipid is able, at physiological concentrations, to completely inhibit sperm motili...

journal_title：Molecular human reproduction

authors： Keller F,Togni G,Soldati G,Balmelli T,Medici G,Rose K,Balerna M

更新日期：1997-03-01 00:00:00

abstract：:Pregnancy-associated plasma protein-A and -A2 (PAPP-A and -A2) are proteases that cleave insulin-like growth factor-binding proteins (IGFBPs), resulting in local activation of IGF signaling pathways. Here, we examined PAPP-A and -A2 mRNA and protein levels in placenta and maternal sera from women with pre-eclampsia an...

journal_title：Molecular human reproduction

authors： Nishizawa H,Pryor-Koishi K,Suzuki M,Kato T,Kogo H,Sekiya T,Kurahashi H,Udagawa Y

更新日期：2008-10-01 00:00:00

abstract：:Olfactory receptors are G protein-coupled transmembrane receptors. Genes encoding olfactory receptors constitute a large gene family of approximately 1000 total member genes. In mammals, a subset of member genes is specifically expressed in the testis (and not in the olfactory mucosa) and olfactory receptor proteins h...

journal_title：Molecular human reproduction

authors： Goto T,Salpekar A,Monk M

更新日期：2001-06-01 00:00:00

abstract：:Controlling the sex of offspring by the separation of X and Y chromosome-bearing spermatozoa using flow cytometry has been reported as a clinical technique aiding prevention of X-linked diseases. Although this technique has resulted in several hundred normal births in animals and at least one human birth, there is sti...

journal_title：Molecular human reproduction

authors： Catt SL,Sakkas D,Bizzaro D,Bianchi PG,Maxwell WM,Evans G

更新日期：1997-09-01 00:00:00

abstract：:The aim of this study was to investigate patterns of gene expression in placental samples from patients with preeclampsia (PE), persistent bilateral uterine artery notching (without PE), and normal controls. This study included placental tissue from nine women with PE, seven with uncomplicated pregnancies and five wit...

journal_title：Molecular human reproduction

authors： Hansson SR,Chen Y,Brodszki J,Chen M,Hernandez-Andrade E,Inman JM,Kozhich OA,Larsson I,Marsál K,Medstrand P,Xiang CC,Brownstein MJ

更新日期：2006-03-01 00:00:00

abstract：:In early gestation, the human placental renin-angiotensin system (RAS) is upregulated and plays a role in placental development. Among other functions, signalling through the angiotensin II type 1 receptor (AT1R) initiates proliferation. Many microRNAs (miRNAs) targeting placental RAS mRNAs are downregulated at this t...

journal_title：Molecular human reproduction

authors： Arthurs AL,Lumbers ER,Pringle KG

更新日期：2019-04-01 00:00:00

abstract：:The process of sperm chromatin decondensation occurs when a spermatozoon enters an ovum. Protamine disulphide bonds are reduced to SH and the polycationic protamines combine with the polyanionic egg protein, nucleoplasmin, thus being stripped from DNA which then combines with histones. Defective chromatin decondensati...

journal_title：Molecular human reproduction

authors： Samocha-Bone D,Lewin LM,Weissenberg R,Madgar Y,Soffer Y,Shochat L,Golan R

更新日期：1998-02-01 00:00:00

abstract：:We have developed a competitive reverse transcription-polymerase chain reaction (RT-PCR) sensitive enough to detect and quantify as little as 2-fold differences in gene expression in individual oocytes and embryos throughout human preimplantation development. This RT-PCR assay can be tailored for the examination of an...

journal_title：Molecular human reproduction

authors： Taylor DM,Handyside AH,Ray PF,Dibb NJ,Winston RM,Ao A

更新日期：2001-02-01 00:00:00

abstract：:Indoleamine 2,3-dioxygenase-1 (IDO1) is an intracellular enzyme that catalyses essential amino acid tryptophan along the kynurenine pathway. The aim of this study was to determine the impact of IDO1 expression on the biological characteristic of the endometrial stromal cells (ESCs). IDO1, cyclooxygenase-2 (COX-2) and ...

journal_title：Molecular human reproduction

authors： Mei J,Jin LP,Ding D,Li MQ,Li DJ,Zhu XY

更新日期：2012-10-01 00:00:00

abstract：:Imprinted genes are characterized by expression of only one of the two alleles according to its inheritance from the mother or the father. This mono-allelic expression must arise from primary differential epigenetic modification of the parental alleles of the imprinted gene in the spermatozoon and the oocyte. Most of ...

journal_title：Molecular human reproduction

authors： Salpekar A,Huntriss J,Bolton V,Monk M

更新日期：2001-09-01 00:00:00

abstract：:Etoposide is widely used in the treatment of patients with testicular cancer. The mechanism underlying apoptosis induction in cancer cells has been studied in different cell types, but it is not known whether the same factors participate in viable germ cells undergoing programmed cell death. Since testicular cancer pr...

journal_title：Molecular human reproduction

authors： Ortiz RJ,Lizama C,Codelia VA,Moreno RD

更新日期：2009-06-01 00:00:00

abstract：:FSH is a pituitary gonadotropin that along with LH plays a key role in the regulation of gonadal function. The gonadotropic hormones are composed of two subunits, the common alpha subunit and the hormone-specific beta subunit, which determines the binding to specific receptors and induction of biological response. Unl...

journal_title：Molecular human reproduction

authors： Lamminen T,Jokinen P,Jiang M,Pakarinen P,Simonsen H,Huhtaniemi I

更新日期：2005-08-01 00:00:00

abstract：:The aim of this study was to determine the effects of tumour necrosis factor alpha (TNF), interleukin-1 alpha (IL-1alpha), macrophage colony-stimulating factor (MCSF) and transforming growth factor beta (TGFbeta) on the secretion of matrix metalloproteinases (MMP), human chorionic gonadotrophin (HCG) and fetal fibrone...

journal_title：Molecular human reproduction

authors： Meisser A,Chardonnens D,Campana A,Bischof P

更新日期：1999-03-01 00:00:00

abstract：:Human pregnancy represents a situation of semiallograft to maternal host. Therefore, it has been reported that tolerance to the fetal allograft represents a mechanism for maintaining a pregnancy. CD4(+)CD25(bright) regulatory T cells are known to play an important role in the development and maintenance of tolerance i...

journal_title：Molecular human reproduction

authors： Sasaki Y,Sakai M,Miyazaki S,Higuma S,Shiozaki A,Saito S

更新日期：2004-05-01 00:00:00

abstract：:Several lines of evidence indicate that mammalian fertilization is initiated via a binding process that is dependent upon the recognition of oligosaccharide sequences associated with zona pellucida (ZP) glycoproteins. Here, specific chemical and enzymatic methods were employed to modify human ZP and to test their effe...

journal_title：Molecular human reproduction

authors： Ozgur K,Patankar MS,Oehninger S,Clark GF

更新日期：1998-04-01 00:00:00

abstract：:This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. Chromatin packing involves disulphide bridge covalent cross-linking. The degree of packi...

journal_title：Molecular human reproduction

authors： Filatov MV,Semenova EV,Vorob'eva OA,Leont'eva OA,Drobchenko EA

更新日期：1999-09-01 00:00:00

abstract：:Because of its distinctive ability to act as a mitogen, a mitogen and a morphogen, hepatocyte growth factor/scatter factor (HGF/SF) has all the characteristics of a molecule able to function in regulatory networks of motility, such as the spermatogenic epithelium, and this through binding of its receptor p190MET (C-ME...

journal_title：Molecular human reproduction

authors： Depuydt CE,Zalata A,de Potter CR,van Emmelo J,Comhaire FH

更新日期：1996-01-01 00:00:00

abstract：:Gonadotrophins exert a major effect on ovarian development and on the control of fertilization. By stimulating cells with forskolin (FK), it is possible to study which genes are activated by gonadotrophins via the cAMP cascade, and which by alternative pathways. Using RNA isolated from stimulated cells, we found that ...

journal_title：Molecular human reproduction

authors： Sasson R,Rimon E,Dantes A,Cohen T,Shinder V,Land-Bracha A,Amsterdam A

更新日期：2004-05-01 00:00:00

abstract：:The aetiology of cryptorchidism is for the most part unknown and appears to be multifactorial. Recently, a product of Leydig cells termed Leydig insulin-like hormone (INSL3) has been proposed as a putative trophic hormone of the first part of descent. Absence of Insl3 in male mice results in bilateral cryptorchidism a...

journal_title：Molecular human reproduction

authors： Krausz C,Quintana-Murci L,Fellous M,Siffroi JP,McElreavey K

更新日期：2000-04-01 00:00:00

abstract：:Genes containing the evolutionarily conserved homeodomain sequence encode a family of DNA-binding transcription factors whose functions are crucial for embryonic development in vertebrates, invertebrates and plants. We describe the detection and analysis of transcripts of homeobox-containing genes present in cDNA libr...

journal_title：Molecular human reproduction

authors： Adjaye J,Monk M

更新日期：2000-08-01 00:00:00

abstract：:The spindle assembly checkpoint modulates the timing of anaphase initiation in response to the improper alignment of chromosomes at the metaphase plate. If defects are detected, a signal is transduced to halt further progression of the cell cycle until correct bipolar attachment to the spindle is achieved. The mitotic...

journal_title：Molecular human reproduction

authors： Steuerwald N,Cohen J,Herrera RJ,Sandalinas M,Brenner CA

更新日期：2001-01-01 00:00:00

abstract：:Human endometrium is the major organ that produces glycodelin A (GdA). The production of endometrial GdA causes a fluctuation of the peripheral glycodelin concentrations in women during the menstrual cycle and pregnancy. It has recently been reported that the rise of plasma concentrations of glycodelin is correlated w...

journal_title：Molecular human reproduction

authors： Tseng L,Zhu HH,Mazella J,Koistinen H,Seppälä M

更新日期：1999-04-01 00:00:00

abstract：:The human egg may only be fertilized by one spermatozoon to prevent polyploidy. In most mammals, the primary block to polyspermy occurs at the zona pellucida (ZP). Little is known of the human ZP and the changes occurring following fertilization to prevent polyploidy. Using antibodies directed against synthetic peptid...

journal_title：Molecular human reproduction

authors： Bauskin AR,Franken DR,Eberspaecher U,Donner P

更新日期：1999-06-01 00:00:00