Recombinant expression and affinity purification of a novel epididymal human sperm-binding protein, BSPH1.

abstract：:During folliculogenesis, the gonadotrophin (GTH)-dependent phase begins at the small antral follicle stage and ends with Graafian follicles. In this study, pregnant mare's serum GTH was used to induce GTH-dependent folliculogenesis in mice, following which the developmental events that follicles undergo, as well as th...

journal_title：Molecular human reproduction

authors： Chen Y,Wang X,Yang C,Liu Q,Ran Z,Li X,He C

更新日期：2020-12-10 00:00:00

abstract：:Oocyte quality is one of the most important aspects of in vitro embryo development. Extensive epigenetic programming must occur during oocyte growth and maturation. A specific DNA methylation pattern of the imprinted genes must be established on differentially methylated regions (DMR). The insulin-like growth factor 2...

journal_title：Molecular human reproduction

authors： Fagundes NS,Michalczechen-Lacerda VA,Caixeta ES,Machado GM,Rodrigues FC,Melo EO,Dode MA,Franco MM

更新日期：2011-02-01 00:00:00

abstract：:Intense macrophage infiltration is observed during luteolysis in various animals including women; however, we still do not know how macrophage infiltration into the human corpus luteum (CL) during luteolysis is regulated. In this study, we examined the expression, localization and regulation of an important chemokine ...

journal_title：Molecular human reproduction

authors： Nio-Kobayashi J,Kudo M,Sakuragi N,Kimura S,Iwanaga T,Duncan WC

更新日期：2015-08-01 00:00:00

abstract：:Survivin is a novel inhibitor of apoptosis. It has been reported that survivin is expressed during fetal development and in cancer tissues, but its expression has not been reported in adult tissues. We investigated the expression of survivin in the endometria of women with regular menstrual cycles using reverse transc...

journal_title：Molecular human reproduction

authors： Konno R,Yamakawa H,Utsunomiya H,Ito K,Sato S,Yajima A

更新日期：2000-06-01 00:00:00

abstract：:Fatty acid amide hydrolase (FAAH) is involved in embryo development and implantation. Sex hormones down-modulate FAAH activity in the mouse uterus. However, the regulation of the FAAH gene in the uterus is unknown. Our results showed that FAAH mRNA is localized to uterine epithelial cells and circular myometrium durin...

journal_title：Molecular human reproduction

authors： Xiao AZ,Zhao YG,Duan EK

更新日期：2002-07-01 00:00:00

abstract：:This study assesses the role of progesterone receptor membrane component 1 (PGRMC1) in actions of progesterone (P4) on human myometrium during pregnancy and labour. Myometrial tissues were obtained from non-pregnant patients during hysterectomy or pregnant women undergoing C-section at term and preterm, before and dur...

journal_title：Molecular human reproduction

authors： Wu W,Shi SQ,Huang HJ,Balducci J,Garfield RE

更新日期：2011-04-01 00:00:00

abstract：:Several studies have demonstrated an association between polycystic ovary syndrome (PCOS) and the dinucleotide repeat microsatellite marker D19S884, which is located in intron 55 of the fibrillin-3 (FBN3) gene. Fibrillins, including FBN1 and 2, interact with latent transforming growth factor (TGF)-beta-binding protein...

journal_title：Molecular human reproduction

authors： Prodoehl MJ,Hatzirodos N,Irving-Rodgers HF,Zhao ZZ,Painter JN,Hickey TE,Gibson MA,Rainey WE,Carr BR,Mason HD,Norman RJ,Montgomery GW,Rodgers RJ

更新日期：2009-12-01 00:00:00

abstract：:Genes containing the evolutionarily conserved homeodomain sequence encode a family of DNA-binding transcription factors whose functions are crucial for embryonic development in vertebrates, invertebrates and plants. We describe the detection and analysis of transcripts of homeobox-containing genes present in cDNA libr...

journal_title：Molecular human reproduction

authors： Adjaye J,Monk M

更新日期：2000-08-01 00:00:00

abstract：:SRY directs testicular development. It has been suggested that the only high-mobility group (HMG) box of the SRY is important for the function of this protein; however, other studies have suggested that the N- and C-terminal regions are also involved in this process. Herein, we analysed and compared in vitro the DNA-b...

journal_title：Molecular human reproduction

authors： Sánchez-Moreno I,Coral-Vázquez R,Méndez JP,Canto P

更新日期：2008-06-01 00:00:00

abstract：:We have previously shown that the estrogen responsiveness of the human lactoferrin gene in a transient transfection system is mediated through an imperfect estrogen response element (ERE) and a steroidogenic factor 1 binding element (SFRE) 26 bp upstream from ERE. Reporter constructs containing SFRE and ERE respond to...

journal_title：Molecular human reproduction

authors： Teng CT,Gladwell W,Beard C,Walmer D,Teng CS,Brenner R

更新日期：2002-01-01 00:00:00

abstract：:Within the context of an oocyte in vitro maturation (IVM) program for reproductive treatment, oocyte cumulus complexes (COCs) derived from follicles <6 mm in patients with PCOS were matured in vitro. Key transcripts related to meiotic maturation (FSHR, LHCGR, EGFR, PGR) and oocyte competence (AREG, ADAMTS, HAS2, PTGS2...

journal_title：Molecular human reproduction

authors： Guzman L,Adriaenssens T,Ortega-Hrepich C,Albuz FK,Mateizel I,Devroey P,De Vos M,Smitz J

更新日期：2013-01-01 00:00:00

abstract：:The aetiology of cryptorchidism is for the most part unknown and appears to be multifactorial. Recently, a product of Leydig cells termed Leydig insulin-like hormone (INSL3) has been proposed as a putative trophic hormone of the first part of descent. Absence of Insl3 in male mice results in bilateral cryptorchidism a...

journal_title：Molecular human reproduction

authors： Krausz C,Quintana-Murci L,Fellous M,Siffroi JP,McElreavey K

更新日期：2000-04-01 00:00:00

abstract：:Virtually all testicular germ cell tumours originate from a common precursor, the carcinoma in situ (CIS) cell. The precise nature of the molecular mechanisms leading to CIS remains largely unknown. We performed the first systematic analysis of gene expression in testis with CIS compared to normal testis by the differ...

journal_title：Molecular human reproduction

authors： Hoei-Hansen CE,Nielsen JE,Almstrup K,Hansen MA,Skakkebaek NE,Rajpert-DeMeyts E,Leffers H

更新日期：2004-06-01 00:00:00

abstract：:Developmental abnormalities of human embryos can be visualized in utero using embryoscopy. Our previous embryoscopic and genetic evaluations detected developmental abnormalities in the majority of both euploid (74%) and aneuploid or polyploid (90%) miscarriages. Since we found the pattern of morphological changes to b...

journal_title：Molecular human reproduction

authors： Rajcan-Separovic E,Qiao Y,Tyson C,Harvard C,Fawcett C,Kalousek D,Stephenson M,Philipp T

更新日期：2010-02-01 00:00:00

abstract：:Gonadotrophins exert a major effect on ovarian development and on the control of fertilization. By stimulating cells with forskolin (FK), it is possible to study which genes are activated by gonadotrophins via the cAMP cascade, and which by alternative pathways. Using RNA isolated from stimulated cells, we found that ...

journal_title：Molecular human reproduction

authors： Sasson R,Rimon E,Dantes A,Cohen T,Shinder V,Land-Bracha A,Amsterdam A

更新日期：2004-05-01 00:00:00

abstract：:Mitochondrial DNA (mtDNA) deletions are present in both human oocytes and embryos. It has been found that these tissues contain a mtDNA mutation which is present in high amounts in patients with Kearns-Sayre syndrome (KSS) and progressive external ophthalmoplegia. In the present study, the frequency of this KSS deleti...

journal_title：Molecular human reproduction

authors： Brenner CA,Wolny YM,Barritt JA,Matt DW,Munné S,Cohen J

更新日期：1998-09-01 00:00:00

abstract：:Glycoforms of recombinant human follicle stimulating hormone (rhFSH) (Org 32489, Puregon) were characterized using concanavalin A lectin affinity chromatography to reveal information about the internal carbohydrate complexity (extent of carbohydrate side-chain branching) of the preparations. The rhFSH glycoforms were ...

journal_title：Molecular human reproduction

authors： Harris SD,Anobile CJ,McLoughlin JD,Mitchell R,Lambert A,Robertson WR

更新日期：1996-10-01 00:00:00

abstract：:After meiosis, during the final stages of spermatogenesis, the haploid male genome undergoes major structural changes, resulting in a shift from a nucleosome-based genome organization to the sperm-specific, highly compacted nucleoprotamine structure. Recent data support the idea that region-specific programming of the...

journal_title：Molecular human reproduction

authors： Govin J,Gaucher J,Ferro M,Debernardi A,Garin J,Khochbin S,Rousseaux S

更新日期：2012-01-01 00:00:00

abstract：:It is widely held that the first two blastomeres of mammalian embryos are equally totipotent and that this totipotency belongs to the group of regulative properties. However, this interpretation neglects an important aspect: evidence only came from successful monozygotic twins which can speak only for those pairs of h...

journal_title：Molecular human reproduction

authors： Casser E,Wdowik S,Israel S,Witten A,Schlatt S,Nordhoff V,Boiani M

更新日期：2019-11-30 00:00:00

abstract：STUDY HYPOTHESIS:Does a preferential X chromosome inactivation (XCI) pattern exist in female human pluripotent stem cells (hPSCs) and does the pattern change during long-term culture or upon differentiation? STUDY FINDING:We identified two independent phenomena that lead to aberrant XCI patterns in female hPSC: a rapi...

journal_title：Molecular human reproduction

authors： Geens M,Seriola A,Barbé L,Santalo J,Veiga A,Dée K,Van Haute L,Sermon K,Spits C

更新日期：2016-04-01 00:00:00

abstract：STUDY QUESTION:What is the acceptability of using stem cell-based fertility treatments (SCFT) for different indications according to gynaecologists and the general public? SUMMARY ANSWER:The majority of gynaecologists and the general public accept SCFT for the indications female or male infertility in young heterosexu...

journal_title：Molecular human reproduction

authors： Hendriks S,Dancet EAF,Vliegenthart R,Repping S

更新日期：2017-12-01 00:00:00

abstract：:Several laboratories have independently isolated mitotically active germ cells, termed female germline stem cells or oogonial stem cells (OSCs), from adult mouse ovaries. However, a recent study using Ddx4-Cre;Rosa26 reporter mice concluded that such germ cells do not exist. Given the disparity in conclusions drawn in...

journal_title：Molecular human reproduction

authors： Park ES,Tilly JL

更新日期：2015-01-01 00:00:00

abstract：:Fluorescent in situ hybridization (FISH) studies of human preimplantation embryos have demonstrated a high proportion of chromosomal mosaicism. To investigate the different timings and nature of chromosomal mosaicism, we developed single cell multiplex fluorescent (FL)-PCR to distinguish meiotic and mitotic cell divis...

journal_title：Molecular human reproduction

authors： Katz-Jaffe MG,Trounson AO,Cram DS

更新日期：2004-02-01 00:00:00

abstract：:Although many candidate genes have been studied in pre-eclampsia (PE), the important class of catecholamine receptors that contribute to sympathetic tone and blood pressure regulation has yet to be investigated. We therefore examined the dopamine D4 receptor (DRD4) gene. We performed a prospective family-based study i...

journal_title：Molecular human reproduction

authors： Korobochka R,Gritsenko I,Gonen R,Ebstein RP,Ohel G

更新日期：2006-02-01 00:00:00

abstract：:Zinc dynamics are essential for oocyte meiotic maturation, egg activation, and preimplantation embryo development. During fertilisation and egg activation, the egg releases billions of zinc atoms (Zn2+) in an exocytotic event termed the 'zinc spark'. We hypothesised that this zinc transport and exocytosis is dependent...

journal_title：Molecular human reproduction

authors： Lee HC,Edmonds ME,Duncan FE,O'Halloran TV,Woodruff TK

更新日期：2020-04-24 00:00:00

abstract：:Villous tissues from 30 spontaneous abortions and the same number of artificial abortions were obtained and analysed for the frequency of polyploid cells. Single cell suspensions were made from these tissues without culture and the ploidy of > 100 cells was analysed. Trisomies of chromosomes 17 and 4 have rarely been ...

journal_title：Molecular human reproduction

authors： Horiuchi I,Hashimoto T,Tsuji Y,Shimada H,Furuyama J,Koyama K

更新日期：1997-05-01 00:00:00

abstract：:A novel gene, TSG23/Tsg23, was identified by comparing the expression profiles of human adult and fetal testis using Affymetrix Genechips. RT-PCR analysis from multiple human and mouse tissues indicated TSG23/Tsg23 mRNA was mainly expressed in the testis. In situ hybridization revealed that TSG23/Tsg23 mRNA was locate...

journal_title：Molecular human reproduction

authors： Zhou Y,Qin D,Tang A,Zhou D,Qin J,Yan B,Diao R,Jiang Z,Cai Z,Gui Y

更新日期：2009-04-01 00:00:00

abstract：:Human sperm contain similar amounts of protamine-1 (P1) and protamine-2 (P2). Although an aberrant protamine ratio have been observed in subfertile men, functional evidence is provided by protamine knockout mice exhibiting male infertility. As sperm DNA integrity is known to be linked with DNA fragmentation and apopto...

journal_title：Molecular human reproduction

authors： Cavalcanti MC,Steilmann C,Failing K,Bergmann M,Kliesch S,Weidner W,Steger K

更新日期：2011-07-01 00:00:00

abstract：:The signals initiating the growth of primordial follicles are unknown. Growth factors such as neurotrophin 4/5 (NT-4/5) and brain-derived neurotrophic factor (BDNF) may play a role in this process. To investigate the expression of NT-4/5 and BDNF and their receptor tyrosine kinase B (TrkB) in the early developing foll...

journal_title：Molecular human reproduction

authors： Harel S,Jin S,Fisch B,Feldberg D,Krissi H,Felz C,Freimann S,Tan SL,Ao A,Abir R

更新日期：2006-06-01 00:00:00

abstract：:The endometrium plays a key role in reproduction, and this function is tightly regulated by endogenous and xenobiotic steroids. Sulphation, catalysed by members of the sulphotransferase (SULT) enzyme family, is a major deactivating mechanism for steroid hormones and we have investigated the expression and regulation i...

journal_title：Molecular human reproduction

authors： Rubin GL,Harrold AJ,Mills JA,Falany CN,Coughtrie MW

更新日期：1999-11-01 00:00:00