Abstract:
:Mitochondrial DNA (mtDNA) deletions are present in both human oocytes and embryos. It has been found that these tissues contain a mtDNA mutation which is present in high amounts in patients with Kearns-Sayre syndrome (KSS) and progressive external ophthalmoplegia. In the present study, the frequency of this KSS deletion was investigated in human oocytes and embryos. Using a nested primer polymerase chain chian reaction (PCR) strategy, the frequency of the KSS deletion in 74 human oocytes and 137 embryos was found to be 32.8 and 8.0% respectively. Using a 'long PCR-short PCR' nested primer strategy, the frequency of the KSS deletion in 181 human oocytes and 104 embryos was found to be 47.0 and 20.2% respectively. There was no statistical correlation between the age of the patients at the time of oocyte retrieval and the presence of the deleted molecules. There was a statistical difference between the presence of the deleted molecules in oocytes versus embryos using either technique (P < 0.0001). The relevance of these findings to the accumulation of low levels of deleted mtDNA in both oocytes and embryos is discussed in this study.
journal_name
Mol Hum Reprodjournal_title
Molecular human reproductionauthors
Brenner CA,Wolny YM,Barritt JA,Matt DW,Munné S,Cohen Jdoi
10.1093/molehr/4.9.887subject
Has Abstractpub_date
1998-09-01 00:00:00pages
887-92issue
9eissn
1360-9947issn
1460-2407journal_volume
4pub_type
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journal_title:Molecular human reproduction
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pub_type: 杂志文章,评审
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更新日期:2016-03-01 00:00:00
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