Abstract:
:Genes containing the evolutionarily conserved homeodomain sequence encode a family of DNA-binding transcription factors whose functions are crucial for embryonic development in vertebrates, invertebrates and plants. We describe the detection and analysis of transcripts of homeobox-containing genes present in cDNA libraries generated from human unfertilized oocytes, single cleavage stage embryos (2-cell, 4-cell, 8-cell and blastocyst) and a 10-week old whole fetus. Using degenerate primers derived from sequences within helix 1 and helix 3 of the highly conserved region of the ANTENNAPEDIA:-class homeodomain, a 166 bp band was detected in all the cDNA libraries tested. Subcloning of the oocyte-derived band revealed that it contained a heterogeneous group of 166 bp fragments. Sequence analysis of 40 independent clones demonstrated the presence of HOXA7, HOXD8, and HOXD1 sequences, the ubiquitously expressed POU family member, OCT1, and HEX, a homeotic gene expressed in haematopoietic cells.
journal_name
Mol Hum Reprodjournal_title
Molecular human reproductionauthors
Adjaye J,Monk Mdoi
10.1093/molehr/6.8.707subject
Has Abstractpub_date
2000-08-01 00:00:00pages
707-11issue
8eissn
1360-9947issn
1460-2407journal_volume
6pub_type
杂志文章abstract::Before the advent of intracytoplasmic sperm injection (ICSI) semen preparation techniques focused on the need to sustain the fertilizing potential of the spermatozoa particularly by reducing oxidative stress. However, for severely oligozoospermic patients treated by ICSI, sperm preparation protocols are used which aim...
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更新日期:2010-10-01 00:00:00
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pub_type: 临床试验,杂志文章
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更新日期:2001-10-01 00:00:00
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更新日期:2004-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/molehr/3.10.827
更新日期:1997-10-01 00:00:00
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更新日期:2008-12-01 00:00:00
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更新日期:2017-10-01 00:00:00
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更新日期:1996-08-01 00:00:00
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更新日期:2000-05-01 00:00:00
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更新日期:1996-09-01 00:00:00
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