Apoptotic gene expression in potentially fertile and subfertile men.


:Human sperm contain similar amounts of protamine-1 (P1) and protamine-2 (P2). Although an aberrant protamine ratio have been observed in subfertile men, functional evidence is provided by protamine knockout mice exhibiting male infertility. As sperm DNA integrity is known to be linked with DNA fragmentation and apoptosis, we investigated whether the DNA fragmentation factor 40 (DFF40) ratio or caspase (Casp4, Casp6) and tumor necrosis factor superfamily member 10 (TNFSF10) ratio together with the P1/P2 ratio represents a reliable biomarker to discriminate between fertile and subfertile men. Real-time quantitative RT-PCR was used for amplification of P1, P2 and DFF40 in 49 testicular biopsies. Casp4, Casp6 and TNFSF10 have been selected from a PCR apoptosis array and were further investigated in another group of testicular biopsies (22 subfertile men versus 11 potentially fertile men). Using Spearman's rank correlation coefficient analysis, we did not find a correlation between DFF40 and P1, P2, P1/P2, score, fertilization rate and age. In addition, logistic regression analysis demonstrated no statistically significant effect of the analyzed variables on pregnancy. A two-way analysis of variance with repeated measures of relative expression of Casp4, Casp6 and TNFSF10 versus P1 or P2 in potentially fertile men and subfertile patients demonstrated statistically significant differences between both groups, all tested gene combinations and the interaction between two genes and both groups in all cases analyzed. Furthermore, significant differences in the expression of Casp4 and TNFSF10 between the groups of potentially fertile and subfertile men could be demonstrated. In addition, the means of differences of selected gene combinations revealed that the protamine to apoptotic gene ratio is statistically different between both groups. Our data suggest that Casp4, Casp 6 and TNFSF10 are differentially expressed in potentially fertile and subfertile men and represent useful biomarkers for predicting male fertility in combination with P1 and P2.


Mol Hum Reprod


Cavalcanti MC,Steilmann C,Failing K,Bergmann M,Kliesch S,Weidner W,Steger K




Has Abstract


2011-07-01 00:00:00














  • SPAG4L/SPAG4L-2 are testis-specific SUN domain proteins restricted to the apical nuclear envelope of round spermatids facing the acrosome.

    abstract::SUN domain proteins are integral proteins of the inner nuclear membrane and functions in linkage of the nuclear lamina to the cytoskeleton. Moreover, SUN domain proteins seem to mediate the tethering of the centrosome to the nuclear membrane, and they are involved in telomere attachment to the nuclear envelope in meio...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Frohnert C,Schweizer S,Hoyer-Fender S

    更新日期:2011-04-01 00:00:00

  • Asthenoteratozoospermia in mice lacking testis expressed gene 18 (Tex18).

    abstract::Testis expressed gene 18 (Tex18) is a small gene with one exon of 240 bp, which is specifically expressed in male germ cells. The gene encodes for a protein of 80 amino acids with unknown domain. To investigate the function of (Tex18) gene, we generated mice with targeted disruption of the (Tex18) gene by homologous r...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Jaroszynski L,Dev A,Li M,Meinhardt A,de Rooij DG,Mueller C,Böhm D,Wolf S,Adham IM,Wulf G,Engel W,Nayernia K

    更新日期:2007-03-01 00:00:00

  • Methylation status in the intragenic differentially methylated region of the IGF2 locus in Bos taurus indicus oocytes with different developmental competencies.

    abstract::Oocyte quality is one of the most important aspects of in vitro embryo development. Extensive epigenetic programming must occur during oocyte growth and maturation. A specific DNA methylation pattern of the imprinted genes must be established on differentially methylated regions (DMR). The insulin-like growth factor 2...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Fagundes NS,Michalczechen-Lacerda VA,Caixeta ES,Machado GM,Rodrigues FC,Melo EO,Dode MA,Franco MM

    更新日期:2011-02-01 00:00:00

  • Nitric oxide synthase distribution during implantation in the mouse.

    abstract::The peri-implantation period is a critical time during murine development. Although the importance of nitric oxide has been demonstrated during gestation, its role in implantation has not been fully defined. The aim of this study was to quantify (by Western blotting) two prominent nitric oxide synthase (NOS) isoforms,...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Purcell TL,Given R,Chwalisz K,Garfield RE

    更新日期:1999-05-01 00:00:00

  • Fas-associated factor (FAF1) is required for the early cleavage-stages of mouse embryo.

    abstract::FAF1 was initially isolated as a Fas-associated factor and was subsequently found to interact with a subset of additional proteins that are involved in many cellular events including Fas-mediated apoptosis, heat shock signalling pathways and ubiquitin-dependent processes. Here, we describe that the 74-kDa FAF1 is ubiq...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Adham IM,Khulan J,Held T,Schmidt B,Meyer BI,Meinhardt A,Engel W

    更新日期:2008-04-01 00:00:00

  • Identification and localization of prostaglandin E2 receptors in upper and lower segment human myometrium during pregnancy.

    abstract::Prostaglandin E2 (PGE2) plays a key role in the maintenance of human pregnancy and labour onset. PGE2 can elicit diverse actions within the uterus depending on the PGE2 receptors (EP1, EP2, EP3 and EP4) expressed. By signalling through different intracellular pathways the EP receptors may inhibit or promote smooth mus...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Astle S,Thornton S,Slater DM

    更新日期:2005-04-01 00:00:00

  • In vivo blockade of α1-adrenergic receptors mitigates stress-disturbed cAMP and cGMP signaling in Leydig cells.

    abstract::The molecular mechanism of stress-associated reproductive dysfunction is complex and largely unknown. This study was designed to systematically analyze molecular effects of systemic in vivo blockade of α1-adrenergic receptors (α1-ADRs) on stress-induced disturbance of cAMP/cGMP signaling in testosterone-producing Leyd...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Stojkov NJ,Baburski AZ,Bjelic MM,Sokanovic SJ,Mihajlovic AI,Drljaca DM,Janjic MM,Kostic TS,Andric SA

    更新日期:2014-01-01 00:00:00

  • Immortalization of normal human cytotrophoblast cells by reconstitution of telomeric reverse transcriptase activity.

    abstract::Placental trophoblast cells are unique endocrine cells that play vital roles during the processes of embryonic implantation and placentation. However, research into the function of human trophoblast has been largely restrained mainly due to a lack of adequate cell models. A normal placenta-origin cytotrophoblast cell ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Wang YL,Qiu W,Feng HC,Li YX,Zhuang LZ,Wang Z,Liu Y,Zhou JQ,Zhang DH,Tsao GS

    更新日期:2006-07-01 00:00:00

  • MicroRNA mimics that target the placental renin-angiotensin system inhibit trophoblast proliferation.

    abstract::In early gestation, the human placental renin-angiotensin system (RAS) is upregulated and plays a role in placental development. Among other functions, signalling through the angiotensin II type 1 receptor (AT1R) initiates proliferation. Many microRNAs (miRNAs) targeting placental RAS mRNAs are downregulated at this t...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Arthurs AL,Lumbers ER,Pringle KG

    更新日期:2019-04-01 00:00:00

  • Induction of superoxide dismutase by decidualization in human endometrial stromal cells.

    abstract::The present study was undertaken to investigate the effect of decidualization on superoxide dismutase (SOD) expression in human endometrial stromal cells (ESC). To induce decidualization, isolated ESC were incubated with medroxyprogesterone acetate (MPA, 10(-6) mol/l) and oestradiol (10(-8) mol/l) for 23 days. Insulin...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Sugino N,Kashida S,Takiguchi S,Nakamura Y,Kato H

    更新日期:2000-02-01 00:00:00

  • Nuclear structural conditions and PCR amplification in human preimplantation diagnosis.

    abstract::An understanding of the relationship between nuclear morphology and DNA function is important in cytology and preimplantation diagnosis. In this study, direct polymerase chain reaction (PCR) amplification was used to diagnose the common delta F508 mutation of cystic fibrosis in 62 biopsied human embryo cells. The nucl...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Cui KH,Matthews CD

    更新日期:1996-01-01 00:00:00

  • Relationship between abnormal sperm chromatin packing and IVF results.

    abstract::This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. Chromatin packing involves disulphide bridge covalent cross-linking. The degree of packi...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Filatov MV,Semenova EV,Vorob'eva OA,Leont'eva OA,Drobchenko EA

    更新日期:1999-09-01 00:00:00

  • MCAK is present at centromeres, midspindle and chiasmata and involved in silencing of the spindle assembly checkpoint in mammalian oocytes.

    abstract::Mitotic centromere-associated kinesin (MCAK) is an ATP-dependent microtubule (MT) depolymerase regulated by Aurora kinase (AURK) phosphorylation and implicated in resolution of improper MT attachments in mitosis. Distribution of MCAK was studied in oocyte maturation by anti-MCAK antibody, anti-tubulin antibody, anti-A...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Vogt E,Sanhaji M,Klein W,Seidel T,Wordeman L,Eichenlaub-Ritter U

    更新日期:2010-09-01 00:00:00

  • Dysregulation of the ADAM17/Notch signalling pathways in endometriosis: from oxidative stress to fibrosis.

    abstract:STUDY QUESTION:Is oxidative stress associated with the A disintegrin and metalloproteases (ADAM) metallopeptidase domain 17 (ADAM17)/Notch signalling pathway and fibrosis in the development of endometriosis? SUMMARY ANSWER:Oxidative stress is correlated with hyperactivation of the ADAM17/Notch signalling pathway and a...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: González-Foruria I,Santulli P,Chouzenoux S,Carmona F,Chapron C,Batteux F

    更新日期:2017-07-01 00:00:00

  • Sperm fucosyltransferase-5 mediates spermatozoa-oviductal epithelial cell interaction to protect human spermatozoa from oxidative damage.

    abstract::Oxidative damage by reactive oxygen species (ROS) is a major cause of sperm dysfunction. Excessive ROS generation reduces fertilization and enhances DNA damage of spermatozoa. Interaction between spermatozoa and oviductal epithelial cells improves the fertilizing ability of and reduces chromatin damage in spermatozoa....

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Huang VW,Lee CL,Lee YL,Lam KK,Ko JK,Yeung WS,Ho PC,Chiu PC

    更新日期:2015-06-01 00:00:00

  • Genetic and gene expression analyses of the polycystic ovary syndrome candidate gene fibrillin-3 and other fibrillin family members in human ovaries.

    abstract::Several studies have demonstrated an association between polycystic ovary syndrome (PCOS) and the dinucleotide repeat microsatellite marker D19S884, which is located in intron 55 of the fibrillin-3 (FBN3) gene. Fibrillins, including FBN1 and 2, interact with latent transforming growth factor (TGF)-beta-binding protein...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Prodoehl MJ,Hatzirodos N,Irving-Rodgers HF,Zhao ZZ,Painter JN,Hickey TE,Gibson MA,Rainey WE,Carr BR,Mason HD,Norman RJ,Montgomery GW,Rodgers RJ

    更新日期:2009-12-01 00:00:00

  • Hoechst staining and exposure to UV laser during flow cytometric sorting does not affect the frequency of detected endogenous DNA nicks in abnormal and normal human spermatozoa.

    abstract::Controlling the sex of offspring by the separation of X and Y chromosome-bearing spermatozoa using flow cytometry has been reported as a clinical technique aiding prevention of X-linked diseases. Although this technique has resulted in several hundred normal births in animals and at least one human birth, there is sti...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Catt SL,Sakkas D,Bizzaro D,Bianchi PG,Maxwell WM,Evans G

    更新日期:1997-09-01 00:00:00

  • Thrombopoietin modulates the proliferation, migration and cytokine profile of decidual cell subsets during early gestation.

    abstract::During early gestation, a considerable increase in different leukocyte subsets can be observed in the decidualized endometrium concomitantly to the invasion of cytotrophoblast cells (CTB). To date, it is still in question which factors induce this accumulation of immune cells and whether it is evoked by an in situ pro...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Segerer SE,Martignoni F,Bogdan A,Müller N,Kapp M,Dietl J,Rieger L,Kämmerer U

    更新日期:2013-06-01 00:00:00

  • Expression and regulation of sFRP family members in human granulosa cells.

    abstract::Follicular development and ovulation are major processes in the reproductive system. Understanding their complexity is important to female fertility treatments and the control of reproductive processes. Wnt signaling pathway components were shown to be involved in reproduction in animal models. The secreted frizzled-r...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Maman E,Yung Y,Cohen B,Konopnicki S,Dal Canto M,Fadini R,Kanety H,Kedem A,Dor J,Hourvitz A

    更新日期:2011-07-01 00:00:00

  • Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients.

    abstract::Pregnancy-associated plasma protein-A and -A2 (PAPP-A and -A2) are proteases that cleave insulin-like growth factor-binding proteins (IGFBPs), resulting in local activation of IGF signaling pathways. Here, we examined PAPP-A and -A2 mRNA and protein levels in placenta and maternal sera from women with pre-eclampsia an...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Nishizawa H,Pryor-Koishi K,Suzuki M,Kato T,Kogo H,Sekiya T,Kurahashi H,Udagawa Y

    更新日期:2008-10-01 00:00:00

  • Differential cell death decisions in the testis: evidence for an exclusive window of ferroptosis in round spermatids.

    abstract::Oxidative stress is a major aetiology in many pathologies, including that of male infertility. Recent evidence in somatic cells has linked oxidative stress to the induction of a novel cell death modality termed ferroptosis. However, the induction of this iron-regulated, caspase-independent cell death pathway has never...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Bromfield EG,Walters JLH,Cafe SL,Bernstein IR,Stanger SJ,Anderson AL,Aitken RJ,McLaughlin EA,Dun MD,Gadella BM,Nixon B

    更新日期:2019-05-01 00:00:00

  • Large scale validation of human N-myc downstream-regulated gene (NDRG)-1 expression in endometrium during the menstrual cycle.

    abstract::A major challenge in the comprehension of the endometrial transformations leading to the completion of each menstrual cycle in humans is in the identification of specific molecular pathways underlying these monthly turnovers. Towards this goal we compared, by the differential display technique, the relative expression...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Malette B,Cherry E,Lagacé M,Bernard M,Gosselin D,Hugo P,Shazand K

    更新日期:2003-11-01 00:00:00

  • Regulation of sulphotransferase expression in the endometrium during the menstrual cycle, by oral contraceptives and during early pregnancy.

    abstract::The endometrium plays a key role in reproduction, and this function is tightly regulated by endogenous and xenobiotic steroids. Sulphation, catalysed by members of the sulphotransferase (SULT) enzyme family, is a major deactivating mechanism for steroid hormones and we have investigated the expression and regulation i...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Rubin GL,Harrold AJ,Mills JA,Falany CN,Coughtrie MW

    更新日期:1999-11-01 00:00:00

  • A 60-66 kDa protein with gonadotrophin surge attenuating factor bioactivity is produced by human ovarian granulosa cells.

    abstract::We aimed to confirm the ovarian site of gonadotrophin surge-attenuating factor (GnSAF) production and produce granulosa/luteal cell-conditioned medium (G/LCM) containing GnSAF for purification studies. Blue dye affinity chromatography followed by pseudochromatofocusing of G/LCM yielded bioactive fractions at pH 5.74 a...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Fowler PA,Sorsa-Leslie T,Cash P,Dunbar B,Melvin W,Wilson Y,Mason HD,Harris W

    更新日期:2002-09-01 00:00:00

  • Functional evidence for divergent receptor activation mechanisms of luteotrophic and luteolytic events in the human corpus luteum.

    abstract::Using a dispersed human luteal cell culture model, progesterone synthesis following treatment by incremental doses of human chorionic gonadotrophin (HCG) and the stable prostaglandin F2alpha (PGF2alpha) analogue cloprostenol, alone or in combination, was related to corpora lutea (CL) mRNA transcript abundance coding f...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Ottander U,Leung CH,Olofsson JI

    更新日期:1999-05-01 00:00:00

  • Zona pellucida-binding protein 2 (ZPBP2) and several proteins containing BX7B motifs in human sperm may have hyaluronic acid binding or recognition properties.

    abstract:STUDY QUESTION:Are there novel hyaladherins in human sperm? SUMMARY ANSWER:Zona pellucida-binding protein 2 (ZPBP2), containing a Link-like hyaluronic acid (HA)-binding domain, and several other proteins containing BX7B motifs, such as ADAM32 and Midkine, may be novel hyaladherins with HA-binding properties. WHAT IS ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Torabi F,Bogle OA,Estanyol JM,Oliva R,Miller D

    更新日期:2017-12-01 00:00:00

  • Characterization and isolation of SOB2, a human sperm protein with a potential role in oocyte membrane binding.

    abstract::G12 monoclonal antibody (mAb), one of a library of constructed mAb directed against human sperm proteins, was found by immunoperoxidase staining to label the post-acrosomal and neck regions of fixed human cauda epididymal and ejaculated spermatozoa. Epithelium and fluid of caput epididymis were strongly labelled while...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Lefèvre A,Martin Ruiz C,Chokomian S,Duquenne C,Finaz C

    更新日期:1997-06-01 00:00:00

  • Polymorphisms of genes involved in innate immunity: association with preterm delivery.

    abstract::An altered inflammatory activity due to functionally relevant polymorphisms of the innate immune system may influence pathways leading to labour and, therefore, impact on the frequency of preterm birth. We examined five polymorphisms of the innate immune system in a large cohort of preterm very-low-birth-weight (VLBW,...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Härtel Ch,Finas D,Ahrens P,Kattner E,Schaible T,Müller D,Segerer H,Albrecht K,Möller J,Diedrich K,Göpel W,Genetic Factors in Neonatology Study Group.

    更新日期:2004-12-01 00:00:00

  • High xylosyltransferase activities in human follicular fluid and cultured granulosa-lutein cells.

    abstract::Follicular fluid proteoglycans play an important role in human oocyte maturation, including the development of a fluid-filled compartment and maintenance of the hypocoagulative state of the follicular fluid. Human xylosyltransferase (EC, XT) is the key enzyme in the biosynthesis of glycosaminoglycan chains in...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Götting C,Kuhn J,Tinneberg HR,Brinkmann T,Kleesiek K

    更新日期:2002-12-01 00:00:00

  • Internal carbohydrate complexity of the oligosaccharide chains of recombinant human follicle stimulating hormone (Puregon, Org 32489): a comparison with Metrodin and Metrodin-HP.

    abstract::Glycoforms of recombinant human follicle stimulating hormone (rhFSH) (Org 32489, Puregon) were characterized using concanavalin A lectin affinity chromatography to reveal information about the internal carbohydrate complexity (extent of carbohydrate side-chain branching) of the preparations. The rhFSH glycoforms were ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Harris SD,Anobile CJ,McLoughlin JD,Mitchell R,Lambert A,Robertson WR

    更新日期:1996-10-01 00:00:00