Relationship between abnormal sperm chromatin packing and IVF results.

Abstract:

:This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. Chromatin packing involves disulphide bridge covalent cross-linking. The degree of packing was determined from the accessibility of DNA to a fluorescent dye, ethidium bromide, following detergent treatment of the spermatozoa. The amount of dye bound was determined by flow cytometry in the presence or absence of heparin, a polyanion which removes only non-disulphide bridge-linked proteins. The results of a number of different sperm samples were compared with their results following in-vitro fertilization, and a relationship between disordered sperm chromatin packing and rates of embryo cleavage was observed. This study suggests that abnormal chromatin packing in spermatozoa may contribute to male fertility.

journal_name

Mol Hum Reprod

authors

Filatov MV,Semenova EV,Vorob'eva OA,Leont'eva OA,Drobchenko EA

doi

10.1093/molehr/5.9.825

subject

Has Abstract

pub_date

1999-09-01 00:00:00

pages

825-30

issue

9

eissn

1360-9947

issn

1460-2407

journal_volume

5

pub_type

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