Abstract:
:This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. Chromatin packing involves disulphide bridge covalent cross-linking. The degree of packing was determined from the accessibility of DNA to a fluorescent dye, ethidium bromide, following detergent treatment of the spermatozoa. The amount of dye bound was determined by flow cytometry in the presence or absence of heparin, a polyanion which removes only non-disulphide bridge-linked proteins. The results of a number of different sperm samples were compared with their results following in-vitro fertilization, and a relationship between disordered sperm chromatin packing and rates of embryo cleavage was observed. This study suggests that abnormal chromatin packing in spermatozoa may contribute to male fertility.
journal_name
Mol Hum Reprodjournal_title
Molecular human reproductionauthors
Filatov MV,Semenova EV,Vorob'eva OA,Leont'eva OA,Drobchenko EAdoi
10.1093/molehr/5.9.825subject
Has Abstractpub_date
1999-09-01 00:00:00pages
825-30issue
9eissn
1360-9947issn
1460-2407journal_volume
5pub_type
杂志文章abstract::An understanding of the relationship between nuclear morphology and DNA function is important in cytology and preimplantation diagnosis. In this study, direct polymerase chain reaction (PCR) amplification was used to diagnose the common delta F508 mutation of cystic fibrosis in 62 biopsied human embryo cells. The nucl...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/2.1.63
更新日期:1996-01-01 00:00:00
abstract::It is widely held that the first two blastomeres of mammalian embryos are equally totipotent and that this totipotency belongs to the group of regulative properties. However, this interpretation neglects an important aspect: evidence only came from successful monozygotic twins which can speak only for those pairs of h...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaz051
更新日期:2019-11-30 00:00:00
abstract::The epithelium lining the epididymis in the male reproductive tract maintains a luminal environment that promotes sperm cell maturation. This process is dependent on the coordinated expression of many genes that encode proteins with a role in epithelial transport. We previously generated genome-wide maps of open chrom...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gau075
更新日期:2014-12-01 00:00:00
abstract::The distribution and size of a surface membrane antigen identified by a monoclonal antibody (MAC9393) have been examined in testicular and epididymal bovine sperm preparations. Western blots indicated a substantial decrease in molecular mass of the antigen during epididymal maturation from approximately 87 kDa in the ...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/4.7.673
更新日期:1998-07-01 00:00:00
abstract:STUDY QUESTION:Does a heterozygous mutation in AMHR2, identified in whole-exome sequencings (WES) of patients with primary ovarian insufficiency (POI), cause a defect in anti-Müllerian hormone (AMH) signaling? SUMMARY ANSWER:The I209N mutation at the adenosine triphosphate binding domain of AMHR2 exerts dominant negat...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaw040
更新日期:2016-09-01 00:00:00
abstract::Previous studies have implicated receptor tyrosine kinases in progesterone-induced [Ca2+]i signalling, and consequent induction of the acrosome reaction, in human spermatozoa. We have investigated the effects of tyrosine kinase inhibition on [Ca2+]i responses in large numbers of individual human spermatozoa. Genistein...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/8.4.326
更新日期:2002-04-01 00:00:00
abstract::Ovarian surface epithelial (OSE) cells participate in the formation of the ovarian cortex and are potential targets of oestrogen action. Oestrogens typically act through nuclear oestrogen receptors (ER) of which there are two known subtypes: ERalpha and ERbeta. In view of the potential importance of oestrogen as a loc...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/4.8.811
更新日期:1998-08-01 00:00:00
abstract:STUDY QUESTION:Does A20 regulate mediators involved in the terminal processes of human labour in primary myometrial and amnion cells? SUMMARY ANSWER:A20 is a nuclear factor-kappa B (NF-κB) responsive gene that acts as a negative regulator of NF-κB-induced expression of pro-labour mediators. WHAT IS KNOWN ALREADY:Infl...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gax041
更新日期:2017-09-01 00:00:00
abstract::We report an improvement in the PGD test for fragile X syndrome (FXS). Recently, multiple displacement amplification (MDA) has been reported to yield large amounts of DNA from single cells. Taking into account this technique, we developed a new PGD test for FXS, enabling combined analysis of linked polymorphic markers...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gal069
更新日期:2006-10-01 00:00:00
abstract::No phenotypic effect is observed in most inversion heterozygotes. However, reproductive risks may occur in the form of infertility, spontaneous abortions or chromosomally unbalanced children as a consequence of meiotic recombination between inverted and non-inverted chromosomes. An odd number of crossovers within the ...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gal094
更新日期:2007-01-01 00:00:00
abstract::Success rate in human pregnancies is believed to be very low and sex-specific mechanisms may operate in prenatal loss. Assuming a sex-differential in prenatal loss exists, we examined genetic markers in biologically plausible targets in the HLA complex, other immune system-related and iron-regulatory genes in 388 heal...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaq047
更新日期:2010-10-01 00:00:00
abstract::Several laboratories have independently isolated mitotically active germ cells, termed female germline stem cells or oogonial stem cells (OSCs), from adult mouse ovaries. However, a recent study using Ddx4-Cre;Rosa26 reporter mice concluded that such germ cells do not exist. Given the disparity in conclusions drawn in...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gau071
更新日期:2015-01-01 00:00:00
abstract:STUDY QUESTION:Can RNA sequencing of human cumulus cells (CC) reveal molecular pathways involved in the physiology of reproductive aging? STUDY FINDING:Senescent but not young CC activate gene pathways associated with hypoxia and oxidative stress. WHAT IS KNOWN ALREADY:Shifts in socioeconomic norms are resulting in l...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaw038
更新日期:2016-08-01 00:00:00
abstract::Oocytes are usually the largest cells in the body and as such offer unique opportunities for single-cell analysis. Unfortunately, these cells are also some of the rarest in the mammalian female, usually necessitating single-cell analysis. In cases of infertility in humans, determining the quality of the oocyte is ofte...
journal_title:Molecular human reproduction
pub_type: 杂志文章,评审
doi:10.1093/molehr/gav064
更新日期:2016-03-01 00:00:00
abstract::Endometriosis is a gynaecological disease with a certain genetic background, but the locations of possible genomic aberrations are still poorly clarified. Intercellular adhesion molecule-1 (ICAM-1), which is a surface glycoprotein that promotes adhesion in immunological and inflammatory reactions, seems to play a role...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gag002
更新日期:2003-01-01 00:00:00
abstract::Multiple types and subtypes, including splice variants, of cyclic nucleotide phosphodiesterases (PDE) have been shown to be expressed in various tissues and organs. They control the intracellular level of cyclic nucleotides and are involved in hormonal signalling. In human spermatozoa, PDE play an important role in th...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/5.8.732
更新日期:1999-08-01 00:00:00
abstract::Regulation of germ-cell-specific transcription is essential for the differentiation and other physiological processes of germ cells. Germ-cell-specific transcription factors, Sohlh2 and Figla, play key roles in gametogenesis. To elucidate whether an epigenetic mechanism is involved in the controlled expression of Sohl...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gar017
更新日期:2011-09-01 00:00:00
abstract::Sperm are motile cells. Thus, a significant component of the spermatogenic cycle is devoted to the formation of flagellum, a process that must be coordinated to insure proper construction. To document the temporal pattern of flagellar gene expression, we employed real-time PCR to assess changes in accumulation of a co...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gah163
更新日期:2005-04-01 00:00:00
abstract::It is well known that human embryonic stem cells (hESCs) frequently acquire recurrent chromosomal abnormalities, very reminiscent of those found in cancerous cells. Given the parallels between cancer and stem cell biology, we set out to investigate the occurrence of a common form of genome instability in tumors, namel...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gau059
更新日期:2014-10-01 00:00:00
abstract::We recently identified the DPY19L2 gene as the main genetic cause of human globozoospermia. Non-genetically characterized cases of globozoospermia were associated with DNA alterations, suggesting that DPY19L2-dependent globozoospermia may be associated with poor DNA quality. However the origins of such defects have no...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gau099
更新日期:2015-02-01 00:00:00
abstract::Matrix metalloproteinases (MMP), tissue inhibitors of metalloproteinases (TIMP), and MMP-TIMP interactions may contribute to the highly programmed process of embryo implantation. The loss of the delicate MMP-TIMP balance may lead to abnormal implantation. The role of TIMP-4 in mouse implantation has not been reported....
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gag019
更新日期:2003-03-01 00:00:00
abstract:STUDY HYPOTHESIS:Does a preferential X chromosome inactivation (XCI) pattern exist in female human pluripotent stem cells (hPSCs) and does the pattern change during long-term culture or upon differentiation? STUDY FINDING:We identified two independent phenomena that lead to aberrant XCI patterns in female hPSC: a rapi...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gaw004
更新日期:2016-04-01 00:00:00
abstract::Controlling the sex of offspring by the separation of X and Y chromosome-bearing spermatozoa using flow cytometry has been reported as a clinical technique aiding prevention of X-linked diseases. Although this technique has resulted in several hundred normal births in animals and at least one human birth, there is sti...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/3.9.821
更新日期:1997-09-01 00:00:00
abstract::Estrogen and selective estrogen receptor modulators (SERMs) differentially impact endometrial cell function, however, the biological basis of these differences is not established. Deregulated cell adhesion to the extracellular matrix, cell movement and invasion are related to endometrial disorders, such as endometrios...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gap045
更新日期:2009-10-01 00:00:00
abstract::Nitric oxide (NO) is synthesized from L-arginine by a family of enzymes known as the nitric oxide synthases (NOS). We have recently shown a NOS similar to constitutive brain NOS (bNOS) and endothelial NOS (ecNOS) to be present in spermatozoa. The aim of this study is to investigate NO production by human spermatozoa a...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/3.9.755
更新日期:1997-09-01 00:00:00
abstract::The aim of the present study was to investigate the potential role of Toll-like receptor 4 (TLR4) in lipopolysaccharide (LPS)-induced preterm delivery. Intraperitoneal injection of LPS in the presence or absence of previous TLR4 blockade was performed to establish a murine model of preterm delivery. The incidences of ...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gap106
更新日期:2010-04-01 00:00:00
abstract::The non-classical major histocompatibility complex molecule, human leukocyte antigen (HLA)-G, is thought to contribute to maternal immune tolerance and successful placentation during pregnancy. Genetic polymorphisms in HLA-G are known to influence expression levels as well as the relative expression of individual prot...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gas041
更新日期:2013-03-01 00:00:00
abstract::Patients with endometriosis are characterized by the ability of the endometrium to implant and by the peritoneal response to the tissue; angiogenic factors may play a significant role in the aetiology of endometriosis supporting the implantation of ectopic endometrial cells. Vascular endothelial growth factor (VEGF) i...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/6.1.50
更新日期:2000-01-01 00:00:00
abstract::In oocytes, cytoplasmic 3' polyadenylation regulates translational activation of dormant mRNA during meiotic maturation. Thus exogenous proteins are hardly expressed after injection of conventional RNA. To circumvent this, we synthesized a long polyadenylated (approximately 250 A) tail to encode RNA with an enhanced y...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/7.11.1039
更新日期:2001-11-01 00:00:00
abstract::The identification of polymorphisms in genes encoding proinflammatory cytokines that affect transcription or the secretion rate has opened new ways to understand the variation in responses to infection during pregnancy. In this study, human amniochorion carrying hyper-responsive alleles of tumour necrosis factor-alpha...
journal_title:Molecular human reproduction
pub_type: 杂志文章
doi:10.1093/molehr/gag076
更新日期:2003-10-01 00:00:00