Human FSH beta subunit gene is highly conserved.

Abstract:

:FSH is a pituitary gonadotropin that along with LH plays a key role in the regulation of gonadal function. The gonadotropic hormones are composed of two subunits, the common alpha subunit and the hormone-specific beta subunit, which determines the binding to specific receptors and induction of biological response. Unlike the LHbeta gene, shown in earlier studies to harbour several amino acid-altering polymorphisms and mutations, information about the eventual sequence variation of the human FSHbeta subunit is not available. In this study, we made sequence analysis and comparison of polymorphisms found in FSHbeta in two Caucasian populations, the Finns and the Danes. It was found that FSHbeta subunit is highly conserved in these populations. Compared with the published sequences, only three silent polymorphisms were detected in the coding regions of the gene, and the promoter sequence was completely identical with the reported sequence. Two of the polymorphisms found were novel, one in the Finnish and one in the Danish population. The results of the sequence analysis show that the human FSHbeta gene is highly conserved and amino acid changing mutations are apparently extremely rare, at least in the samples collected randomly from control populations. This may be due to the crucial role of normal FSH function in the regulation of fertility.

journal_name

Mol Hum Reprod

authors

Lamminen T,Jokinen P,Jiang M,Pakarinen P,Simonsen H,Huhtaniemi I

doi

10.1093/molehr/gah198

subject

Has Abstract

pub_date

2005-08-01 00:00:00

pages

601-5

issue

8

eissn

1360-9947

issn

1460-2407

pii

gah198

journal_volume

11

pub_type

杂志文章
  • The genotype of the NK cell receptor, KIR2DL4, influences INFgamma secretion by decidual natural killer cells.

    abstract::Natural killer (NK) cells are the predominant leukocyte in first trimester decidua and play a role in vascular remodelling through interferon gamma (IFNgamma) secretion. Membrane expression of the killer immunoglobulin-like receptor (KIR) KIR2DL4 on peripheral blood NK (pNK) cells is controlled by the 9A/10A transmemb...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gap039

    authors: Goodridge JP,Lathbury LJ,John E,Charles AK,Christiansen FT,Witt CS

    更新日期:2009-08-01 00:00:00

  • High concentrations of inhibin A and inhibin B in ovarian serous cystadenoma: relationship with oestradiol and nitric oxide metabolites.

    abstract::Inhibin production has been demonstrated in malignant epithelial ovarian tumours, but secretion of inhibins by benign cystadenoma has not yet been reported. The present study evaluated the concentrations of inhibin A and inhibin B and the relationship with oestradiol and nitric oxide metabolites in fluid collected fro...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/6.12.1079

    authors: Reis FM,Faletti A,Luisi S,Bifulco G,Cauci S,Quadrifoglio F,Dor J,Petraglia F

    更新日期:2000-12-01 00:00:00

  • Differential actions of estrogen and SERMs in regulation of the actin cytoskeleton of endometrial cells.

    abstract::Estrogen and selective estrogen receptor modulators (SERMs) differentially impact endometrial cell function, however, the biological basis of these differences is not established. Deregulated cell adhesion to the extracellular matrix, cell movement and invasion are related to endometrial disorders, such as endometrios...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gap045

    authors: Flamini MI,Sanchez AM,Goglia L,Tosi V,Genazzani AR,Simoncini T

    更新日期:2009-10-01 00:00:00

  • Internal carbohydrate complexity of the oligosaccharide chains of recombinant human follicle stimulating hormone (Puregon, Org 32489): a comparison with Metrodin and Metrodin-HP.

    abstract::Glycoforms of recombinant human follicle stimulating hormone (rhFSH) (Org 32489, Puregon) were characterized using concanavalin A lectin affinity chromatography to reveal information about the internal carbohydrate complexity (extent of carbohydrate side-chain branching) of the preparations. The rhFSH glycoforms were ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/2.10.807

    authors: Harris SD,Anobile CJ,McLoughlin JD,Mitchell R,Lambert A,Robertson WR

    更新日期:1996-10-01 00:00:00

  • Mouse staufen genes are expressed in germ cells during oogenesis and spermatogenesis.

    abstract::The Drosophila melanogaster staufen gene encodes an RNA binding protein (Dm Stau) required for the localization and translational repression of mRNAs within the Drosophila oocyte. In mammals translational repression is important for normal spermatogenesis in males and storage of mRNAs in the oocytes of females. In the...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/6.11.983

    authors: Saunders PT,Pathirana S,Maguire SM,Doyle M,Wood T,Bownes M

    更新日期:2000-11-01 00:00:00

  • Identification of the five most common cystic fibrosis mutations in single cells using a rapid and specific differential amplification system.

    abstract::We describe a rapid and specific differential amplification system which can detect five of the most common cystic fibrosis mutations from a single cell. In the first round of the polymerase chain reaction (PCR), regions of exons 4, 10 and 11 of the cystic fibrosis transmembrane conductance regulator (CFTR) gene conta...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/2.3.203

    authors: Scobie G,Woodroffe B,Fishel S,Kalsheker N

    更新日期:1996-03-01 00:00:00

  • In-vitro human spermatozoa nuclear decondensation assessed by flow cytometry.

    abstract::The process of sperm chromatin decondensation occurs when a spermatozoon enters an ovum. Protamine disulphide bonds are reduced to SH and the polycationic protamines combine with the polyanionic egg protein, nucleoplasmin, thus being stripped from DNA which then combines with histones. Defective chromatin decondensati...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/4.2.133

    authors: Samocha-Bone D,Lewin LM,Weissenberg R,Madgar Y,Soffer Y,Shochat L,Golan R

    更新日期:1998-02-01 00:00:00

  • Patterns of expression of sperm flagellar genes: early expression of genes encoding axonemal proteins during the spermatogenic cycle and shared features of promoters of genes encoding central apparatus proteins.

    abstract::Sperm are motile cells. Thus, a significant component of the spermatogenic cycle is devoted to the formation of flagellum, a process that must be coordinated to insure proper construction. To document the temporal pattern of flagellar gene expression, we employed real-time PCR to assess changes in accumulation of a co...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gah163

    authors: Horowitz E,Zhang Z,Jones BH,Moss SB,Ho C,Wood JR,Wang X,Sammel MD,Strauss JF 3rd

    更新日期:2005-04-01 00:00:00

  • Nuclear and cytoplasmic determinants involved in the regulation of mammalian oocyte maturation.

    abstract::The requisite endpoint of mammalian oocyte maturation, whether in vivo or in vitro, is a metaphase II oocyte which is able to be fertilized and which can eventually support normal embryonic development. Oocytes which have been matured in vivo basically fulfill these criteria. On the other hand, a completely different ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章,评审

    doi:10.1093/molehr/4.1.41

    authors: Fulka J Jr,First NL,Moor RM

    更新日期:1998-01-01 00:00:00

  • MicroRNA mimics that target the placental renin-angiotensin system inhibit trophoblast proliferation.

    abstract::In early gestation, the human placental renin-angiotensin system (RAS) is upregulated and plays a role in placental development. Among other functions, signalling through the angiotensin II type 1 receptor (AT1R) initiates proliferation. Many microRNAs (miRNAs) targeting placental RAS mRNAs are downregulated at this t...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gaz010

    authors: Arthurs AL,Lumbers ER,Pringle KG

    更新日期:2019-04-01 00:00:00

  • Evaluation of stimulus-induced acrosome reaction by two-colour flow cytometric analysis.

    abstract::Acrosome status in human spermatozoa from 20 normozoospermic men was evaluated by flow cytometry following the induction of the acrosome reaction with the ionophore A23187. Dual fluorescence staining of methanol fixed spermatozoa incubated with and without (control) the ionophore A23187 was performed with probes which...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/4.3.243

    authors: Nikolaeva MA,Golubeva EL,Kulakov VI,Sukhikh GT

    更新日期:1998-03-01 00:00:00

  • Mitotic errors in chromosome 21 of human preimplantation embryos are associated with non-viability.

    abstract::Fluorescent in situ hybridization (FISH) studies of human preimplantation embryos have demonstrated a high proportion of chromosomal mosaicism. To investigate the different timings and nature of chromosomal mosaicism, we developed single cell multiplex fluorescent (FL)-PCR to distinguish meiotic and mitotic cell divis...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gah017

    authors: Katz-Jaffe MG,Trounson AO,Cram DS

    更新日期:2004-02-01 00:00:00

  • Genomic imprinting: potential function and mechanisms revealed by the Prader-Willi and Angelman syndromes.

    abstract::The Prader-Willi (PWS) and Angelman (AS) syndromes are two clinically distinct syndromes which result from lack of expression of imprinted genes within chromosome 15q11-q13. These two syndromes result from 15q11-q13 deletions, chromosome 15 uniparental disomy (UPD), imprinting centre mutations and, for AS, probable mu...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章,评审

    doi:10.1093/molehr/3.4.321

    authors: Glenn CC,Driscoll DJ,Yang TP,Nicholls RD

    更新日期:1997-04-01 00:00:00

  • IL-33 enhances proliferation and invasiveness of decidual stromal cells by up-regulation of CCL2/CCR2 via NF-κB and ERK1/2 signaling.

    abstract::Interleukin (IL)-33, a newly described member of the IL-1 family, has been reported to facilitate primary tumor progression and metastatic dissemination. However, its biological function on decidual stromal cells (DSCs) remains unclear. In this study, we tested the hypothesis whether IL-33 promotes proliferation and i...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gat094

    authors: Hu WT,Li MQ,Liu W,Jin LP,Li DJ,Zhu XY

    更新日期:2014-04-01 00:00:00

  • Hoechst staining and exposure to UV laser during flow cytometric sorting does not affect the frequency of detected endogenous DNA nicks in abnormal and normal human spermatozoa.

    abstract::Controlling the sex of offspring by the separation of X and Y chromosome-bearing spermatozoa using flow cytometry has been reported as a clinical technique aiding prevention of X-linked diseases. Although this technique has resulted in several hundred normal births in animals and at least one human birth, there is sti...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/3.9.821

    authors: Catt SL,Sakkas D,Bizzaro D,Bianchi PG,Maxwell WM,Evans G

    更新日期:1997-09-01 00:00:00

  • Surfactant protein D in the female genital tract.

    abstract::Surfactant protein D (SP-D) plays a role in innate immunity against various pathogens and in vivo studies have demonstrated that SP-D also has anti-inflammatory properties. SP-D was originally demonstrated in alveolar type II cells, but recent studies have shown extrapulmonary expression of SP-D indicating a systemic ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gah022

    authors: Leth-Larsen R,Floridon C,Nielsen O,Holmskov U

    更新日期:2004-03-01 00:00:00

  • Genetic and gene expression analyses of the polycystic ovary syndrome candidate gene fibrillin-3 and other fibrillin family members in human ovaries.

    abstract::Several studies have demonstrated an association between polycystic ovary syndrome (PCOS) and the dinucleotide repeat microsatellite marker D19S884, which is located in intron 55 of the fibrillin-3 (FBN3) gene. Fibrillins, including FBN1 and 2, interact with latent transforming growth factor (TGF)-beta-binding protein...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gap072

    authors: Prodoehl MJ,Hatzirodos N,Irving-Rodgers HF,Zhao ZZ,Painter JN,Hickey TE,Gibson MA,Rainey WE,Carr BR,Mason HD,Norman RJ,Montgomery GW,Rodgers RJ

    更新日期:2009-12-01 00:00:00

  • Surface-expressed E-cadherin, and mitochondrial and microtubule distribution in rescue of mouse embryos from 2-cell block by aggregation.

    abstract::E-cadherin (uvomorulin)-mediated cell interactions are essential for preimplantation development in mammals. We observed that E-cadherin is expressed at contact sites between blastomeres of 2-cell mouse embryos of non-blocking genotype (CBA x C57BL F1) explanted at 32 h post human chorionic gonadotrophin (HCG) and cul...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/6.5.454

    authors: Neganova IE,Sekirina GG,Eichenlaub-Ritter U

    更新日期:2000-05-01 00:00:00

  • Identification and characterization of an ascorbic acid transporter in human granulosa-lutein cells.

    abstract::Ascorbic acid serves a vital role as a pre-eminent antioxidant. In animals, it has been shown to be concentrated in granulosa and theca cells of the follicle, in luteal cells of the corpus luteum, and in the peripheral cytoplasm of the oocyte. We have previously identified hormonally-regulated ascorbic acid transporte...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/5.4.299

    authors: Zreik TG,Kodaman PH,Jones EE,Olive DL,Behrman H

    更新日期:1999-04-01 00:00:00

  • Recombinant expression and affinity purification of a novel epididymal human sperm-binding protein, BSPH1.

    abstract::Mammalian sperm undergo a series of maturation steps before acquiring fertilization competence. Our previous work demonstrated the importance of binder of sperm (BSP) proteins in bovine sperm capacitation. Recent studies identified a BSP-homologous DNA sequence in the human genome (BSPH1) and mRNA expression in the ep...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gan077

    authors: Lefebvre J,Boileau G,Manjunath P

    更新日期:2009-02-01 00:00:00

  • Proteomic strategy for the identification of critical actors in reorganization of the post-meiotic male genome.

    abstract::After meiosis, during the final stages of spermatogenesis, the haploid male genome undergoes major structural changes, resulting in a shift from a nucleosome-based genome organization to the sperm-specific, highly compacted nucleoprotamine structure. Recent data support the idea that region-specific programming of the...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gar063

    authors: Govin J,Gaucher J,Ferro M,Debernardi A,Garin J,Khochbin S,Rousseaux S

    更新日期:2012-01-01 00:00:00

  • Genomic RNA profiling and the programme controlling preimplantation mammalian development.

    abstract::Preimplantation development shifts from a maternal to embryonic programme rapidly after fertilization. Although the majority of oogenetic products are lost during the maternal to embryonic transition (MET), several do survive this interval to contribute directly to supporting preimplantation development. Embryonic gen...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gan063

    authors: Bell CE,Calder MD,Watson AJ

    更新日期:2008-12-01 00:00:00

  • Methylation status in the intragenic differentially methylated region of the IGF2 locus in Bos taurus indicus oocytes with different developmental competencies.

    abstract::Oocyte quality is one of the most important aspects of in vitro embryo development. Extensive epigenetic programming must occur during oocyte growth and maturation. A specific DNA methylation pattern of the imprinted genes must be established on differentially methylated regions (DMR). The insulin-like growth factor 2...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gaq075

    authors: Fagundes NS,Michalczechen-Lacerda VA,Caixeta ES,Machado GM,Rodrigues FC,Melo EO,Dode MA,Franco MM

    更新日期:2011-02-01 00:00:00

  • Expression of survivin and Bcl-2 in the normal human endometrium.

    abstract::Survivin is a novel inhibitor of apoptosis. It has been reported that survivin is expressed during fetal development and in cancer tissues, but its expression has not been reported in adult tissues. We investigated the expression of survivin in the endometria of women with regular menstrual cycles using reverse transc...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/6.6.529

    authors: Konno R,Yamakawa H,Utsunomiya H,Ito K,Sato S,Yajima A

    更新日期:2000-06-01 00:00:00

  • Low expression of adhesion molecules and matrix proteins in patients showing poor penetration in zona-free hamster oocytes.

    abstract::The expression of adhesion molecules and matrix proteins by human spermatozoa as well as their binding to and penetration of zona-free hamster eggs were investigated in 17 patients by means of flow cytometry. Both binding and penetration of hamster oocytes and expression of alpha- and beta-chains of beta 1, beta 3 and...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/2.5.335

    authors: Henkel R,Schaller J,Glander HJ,Schill WB

    更新日期:1996-05-01 00:00:00

  • The effect of intrauterine levonorgestrel use on the expression of c-JUN, oestrogen receptors, progesterone receptors and Ki-67 in human endometrium.

    abstract::The major regulators of endometrial function are oestrogen and progesterone, which act through binding their nuclear receptors and by activating transcription of their target genes. Interactions between steroid receptors and transcription proteins, e.g. c-JUN/AP-1, can modulate steroid action at the transcriptional le...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/4.12.1110

    authors: Salmi A,Pakarinen P,Peltola AM,Rutanen EM

    更新日期:1998-12-01 00:00:00

  • Sperm fucosyltransferase-5 mediates spermatozoa-oviductal epithelial cell interaction to protect human spermatozoa from oxidative damage.

    abstract::Oxidative damage by reactive oxygen species (ROS) is a major cause of sperm dysfunction. Excessive ROS generation reduces fertilization and enhances DNA damage of spermatozoa. Interaction between spermatozoa and oviductal epithelial cells improves the fertilizing ability of and reduces chromatin damage in spermatozoa....

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gav015

    authors: Huang VW,Lee CL,Lee YL,Lam KK,Ko JK,Yeung WS,Ho PC,Chiu PC

    更新日期:2015-06-01 00:00:00

  • Symmetry breaking in the early mammalian embryo: the case for quantitative single-cell imaging analysis.

    abstract::In recent years, advances in imaging probes, cutting-edge microscopy techniques and powerful bioinformatics image analysis have markedly expanded the imaging toolbox available to developmental biologists. Apart from traditional qualitative studies, embryonic development can now be investigated in vivo with improved sp...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gav048

    authors: Welling M,Ponti A,Pantazis P

    更新日期:2016-03-01 00:00:00

  • Menstrual blood-derived stromal stem cells from women with and without endometriosis reveal different phenotypic and functional characteristics.

    abstract::Retrograde flow of menstrual blood cells during menstruation is considered as the dominant theory for the development of endometriosis. Moreover, current evidence suggests that endometrial-derived stem cells are key players in the pathogenesis of endometriosis. In particular, endometrial stromal stem cells have been s...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gau044

    authors: Nikoo S,Ebtekar M,Jeddi-Tehrani M,Shervin A,Bozorgmehr M,Vafaei S,Kazemnejad S,Zarnani AH

    更新日期:2014-09-01 00:00:00

  • Stromal cell-derived factor-1 (SDF-1) signalling regulates human placental trophoblast cell survival.

    abstract::Stromal cell-derived factor-1 (SDF-1 or CXCL12) is the physiologic ligand for the chemokine receptor CXCR4. CXCR4-mediated signalling regulates cell migration and apoptosis in certain haematopoietic and neuronal cells. Using gene profiling, we determined that CXCR4 is the only chemokine receptor for which mRNA express...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章

    doi:10.1093/molehr/gah118

    authors: Jaleel MA,Tsai AC,Sarkar S,Freedman PV,Rubin LP

    更新日期:2004-12-01 00:00:00