Abstract:
:We have investigated the possible roles of oncostatin M (OSM), which is a member of the interleukin-6 family of cytokines, in endometrial and endometriotic stromal cell growth. Endometrial and endometriotic stromal cells were collected from the uterus or ovarian chocolate cysts. We observed the expression of mRNA transcripts for OSM, OSM receptor subunit beta, leukaemia inhibitory factor receptor subunit (LIFR), and glycoprotein 130 in endometrial and endometriotic stromal cells. We also examined the effects of OSM (0-50 ng/ml) and LIF (0-10 ng/ml) on endometrial and endometriotic stromal cell proliferation and evaluated the effects of OSM on endometrial stromal cell differentiation. The presence of 10-50 ng/ml OSM significantly suppressed endometrial stromal cell growth in secretory phase tissue but not in proliferative phase tissue. In contrast, stromal cells in endometriotic tissues were resistant to the inhibitory effects of OSM. Addition of LIF did not influence the growth of endometrial stromal cells. We also showed that 10 ng/ml OSM stimulated markers of differentiation causing increased prolactin secretion and cyclooxygenase-2 gene expression in endometrial stromal cells from the secretory phase. These results suggest that OSM may play a pivotal role in regulating the growth and differentiation of endometrial cells. Endometriotic cells may behave differently from normal endometrial cells in terms of the inhibitory response to OSM.
journal_name
Mol Hum Reprodjournal_title
Molecular human reproductionauthors
Ohata Y,Harada T,Fujii A,Yoshida S,Iwabe T,Terakawa Ndoi
10.1093/molehr/7.7.665subject
Has Abstractpub_date
2001-07-01 00:00:00pages
665-70issue
7eissn
1360-9947issn
1460-2407journal_volume
7pub_type
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pub_type: 杂志文章
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更新日期:2002-03-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2016-03-01 00:00:00
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journal_title:Molecular human reproduction
pub_type: 杂志文章,评审
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更新日期:2004-01-01 00:00:00
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pub_type: 杂志文章
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