Developmental origins of inflammatory and immune diseases.

abstract：:After meiosis, during the final stages of spermatogenesis, the haploid male genome undergoes major structural changes, resulting in a shift from a nucleosome-based genome organization to the sperm-specific, highly compacted nucleoprotamine structure. Recent data support the idea that region-specific programming of the...

journal_title：Molecular human reproduction

authors： Govin J,Gaucher J,Ferro M,Debernardi A,Garin J,Khochbin S,Rousseaux S

更新日期：2012-01-01 00:00:00

abstract：STUDY QUESTION:Can supplementation of media with a glutathione (GSH) donor, glutathione ethyl ester (GEE), prior to vitrification protect the mouse oocyte from oxidative damage and critical changes in redox homeostasis, and thereby improve cryotolerance? SUMMARY ANSWER:GEE supplementation supported redox regulation, r...

journal_title：Molecular human reproduction

authors： Trapphoff T,Heiligentag M,Simon J,Staubach N,Seidel T,Otte K,Fröhlich T,Arnold GJ,Eichenlaub-Ritter U

更新日期：2016-12-01 00:00:00

abstract：:Microdeletions within the AZF (azoospermia factor) a, b and c regions of the Y chromosome can be detected worldwide in 1-10% of infertile men. AZFc, containing genes such as DAZ, CDY, RBMY and others, is most frequently deleted and associated with oligo- or azoospermia. The function of the different genes within AZFc ...

journal_title：Molecular human reproduction

authors： Kostova E,Röttger S,Schempp W,Gromoll J

更新日期：2002-08-01 00:00:00

abstract：:Ascorbic acid serves a vital role as a pre-eminent antioxidant. In animals, it has been shown to be concentrated in granulosa and theca cells of the follicle, in luteal cells of the corpus luteum, and in the peripheral cytoplasm of the oocyte. We have previously identified hormonally-regulated ascorbic acid transporte...

journal_title：Molecular human reproduction

authors： Zreik TG,Kodaman PH,Jones EE,Olive DL,Behrman H

更新日期：1999-04-01 00:00:00

abstract：:In the interest of classifying cases of male factor infertility, we have paid particular attention to the sugar ligand binding properties of the human sperm surface and the functional capacity of the acrosome for exocytosis--key parameters for assessing sperm fertilizing ability. Zona recognition and binding involve t...

journal_title：Molecular human reproduction

authors： Benoff S,Hurley IR,Mandel FS,Cooper GW,Hershlag A

更新日期：1997-10-01 00:00:00

abstract：:Glycoforms of recombinant human follicle stimulating hormone (rhFSH) (Org 32489, Puregon) were characterized using concanavalin A lectin affinity chromatography to reveal information about the internal carbohydrate complexity (extent of carbohydrate side-chain branching) of the preparations. The rhFSH glycoforms were ...

journal_title：Molecular human reproduction

authors： Harris SD,Anobile CJ,McLoughlin JD,Mitchell R,Lambert A,Robertson WR

更新日期：1996-10-01 00:00:00

abstract：:We have investigated the possible roles of oncostatin M (OSM), which is a member of the interleukin-6 family of cytokines, in endometrial and endometriotic stromal cell growth. Endometrial and endometriotic stromal cells were collected from the uterus or ovarian chocolate cysts. We observed the expression of mRNA tran...

journal_title：Molecular human reproduction

authors： Ohata Y,Harada T,Fujii A,Yoshida S,Iwabe T,Terakawa N

更新日期：2001-07-01 00:00:00

abstract：STUDY QUESTION:Are there novel hyaladherins in human sperm? SUMMARY ANSWER:Zona pellucida-binding protein 2 (ZPBP2), containing a Link-like hyaluronic acid (HA)-binding domain, and several other proteins containing BX7B motifs, such as ADAM32 and Midkine, may be novel hyaladherins with HA-binding properties. WHAT IS ...

journal_title：Molecular human reproduction

authors： Torabi F,Bogle OA,Estanyol JM,Oliva R,Miller D

更新日期：2017-12-01 00:00:00

abstract：:We purified by fractionation on 10-40% glycerol gradients, 26S proteasomes from normal human spermatozoa. These proteasomes, which participate in the ATP-dependent degradation of ubiquitinated proteins, share a similar sedimentation coefficient to those purified from other human tissues. Fluorogenic peptide assays rev...

journal_title：Molecular human reproduction

authors： Tipler CP,Hutchon SP,Hendil K,Tanaka K,Fishel S,Mayer RJ

更新日期：1997-12-01 00:00:00

abstract：:Using a dispersed human luteal cell culture model, progesterone synthesis following treatment by incremental doses of human chorionic gonadotrophin (HCG) and the stable prostaglandin F2alpha (PGF2alpha) analogue cloprostenol, alone or in combination, was related to corpora lutea (CL) mRNA transcript abundance coding f...

journal_title：Molecular human reproduction

authors： Ottander U,Leung CH,Olofsson JI

更新日期：1999-05-01 00:00:00

abstract：:We recently identified the DPY19L2 gene as the main genetic cause of human globozoospermia. Non-genetically characterized cases of globozoospermia were associated with DNA alterations, suggesting that DPY19L2-dependent globozoospermia may be associated with poor DNA quality. However the origins of such defects have no...

journal_title：Molecular human reproduction

authors： Yassine S,Escoffier J,Martinez G,Coutton C,Karaouzène T,Zouari R,Ravanat JL,Metzler-Guillemain C,Lee HC,Fissore R,Hennebicq S,Ray PF,Arnoult C

更新日期：2015-02-01 00:00:00

abstract：:A novel gene, TSG23/Tsg23, was identified by comparing the expression profiles of human adult and fetal testis using Affymetrix Genechips. RT-PCR analysis from multiple human and mouse tissues indicated TSG23/Tsg23 mRNA was mainly expressed in the testis. In situ hybridization revealed that TSG23/Tsg23 mRNA was locate...

journal_title：Molecular human reproduction

authors： Zhou Y,Qin D,Tang A,Zhou D,Qin J,Yan B,Diao R,Jiang Z,Cai Z,Gui Y

更新日期：2009-04-01 00:00:00

abstract：:In spite of progress in diagnosis and treatment, prostate cancer has become one of the most frequent lethal cancers in males in many Western industrialized countries. Research on the molecular biology of prostate cancer is expected to reveal those aspects of Western lifestyle contributing to its high incidence with th...

journal_title：Molecular human reproduction

authors： Schulz WA,Burchardt M,Cronauer MV

更新日期：2003-08-01 00:00:00

abstract：:Success rate in human pregnancies is believed to be very low and sex-specific mechanisms may operate in prenatal loss. Assuming a sex-differential in prenatal loss exists, we examined genetic markers in biologically plausible targets in the HLA complex, other immune system-related and iron-regulatory genes in 388 heal...

journal_title：Molecular human reproduction

authors： Ucisik-Akkaya E,Davis CF,Do TN,Morrison BA,Stemmer SM,Amadio WJ,Dorak MT

更新日期：2010-10-01 00:00:00

abstract：:This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. Chromatin packing involves disulphide bridge covalent cross-linking. The degree of packi...

journal_title：Molecular human reproduction

authors： Filatov MV,Semenova EV,Vorob'eva OA,Leont'eva OA,Drobchenko EA

更新日期：1999-09-01 00:00:00

abstract：:It is well known that human embryonic stem cells (hESCs) frequently acquire recurrent chromosomal abnormalities, very reminiscent of those found in cancerous cells. Given the parallels between cancer and stem cell biology, we set out to investigate the occurrence of a common form of genome instability in tumors, namel...

journal_title：Molecular human reproduction

authors： Nguyen HT,Markouli C,Geens M,Barbé L,Sermon K,Spits C

更新日期：2014-10-01 00:00:00

abstract：:During early gestation, a considerable increase in different leukocyte subsets can be observed in the decidualized endometrium concomitantly to the invasion of cytotrophoblast cells (CTB). To date, it is still in question which factors induce this accumulation of immune cells and whether it is evoked by an in situ pro...

journal_title：Molecular human reproduction

authors： Segerer SE,Martignoni F,Bogdan A,Müller N,Kapp M,Dietl J,Rieger L,Kämmerer U

更新日期：2013-06-01 00:00:00

abstract：STUDY HYPOTHESIS:It is possible to isolate pure populations of single potential human spermatogonial stem cells without somatic contamination for down-stream applications, for example cell culture and gene expression analysis. STUDY FINDING:We isolated pure populations of single potential human spermatogonial stem cel...

journal_title：Molecular human reproduction

authors： von Kopylow K,Schulze W,Salzbrunn A,Spiess AN

更新日期：2016-04-01 00:00:00

abstract：STUDY HYPOTHESIS:Does a preferential X chromosome inactivation (XCI) pattern exist in female human pluripotent stem cells (hPSCs) and does the pattern change during long-term culture or upon differentiation? STUDY FINDING:We identified two independent phenomena that lead to aberrant XCI patterns in female hPSC: a rapi...

journal_title：Molecular human reproduction

authors： Geens M,Seriola A,Barbé L,Santalo J,Veiga A,Dée K,Van Haute L,Sermon K,Spits C

更新日期：2016-04-01 00:00:00

abstract：:Several reports demonstrating the presence of messenger RNAs for sperm-specific nucleoproteins, the protamines PRM-1, PRM-2, and the transition protein TP-1 as well as beta-actin, c-MYC, HLA1, and beta 1 integrin have challenged the accepted view of the transcriptional dormance of terminally differentiated spermatozoa...

journal_title：Molecular human reproduction

authors： Miller D

更新日期：1997-08-01 00:00:00

abstract：:Controlling the sex of offspring by the separation of X and Y chromosome-bearing spermatozoa using flow cytometry has been reported as a clinical technique aiding prevention of X-linked diseases. Although this technique has resulted in several hundred normal births in animals and at least one human birth, there is sti...

journal_title：Molecular human reproduction

authors： Catt SL,Sakkas D,Bizzaro D,Bianchi PG,Maxwell WM,Evans G

更新日期：1997-09-01 00:00:00

abstract：:The etiology and pathogenesis of endometriosis are complex with both genetic and environmental factors contributing to disease risk. Genome-wide association studies (GWAS) have identified multiple signals in the estrogen receptor 1 (ESR1) region associated with endometriosis and other reproductive traits and diseases....

journal_title：Molecular human reproduction

authors： Marla S,Mortlock S,Houshdaran S,Fung J,McKinnon B,Holdsworth-Carson SJ,Girling JE,Rogers PAW,Giudice LC,Montgomery GW

更新日期：2021-01-22 00:00:00

abstract：:Advanced glycation end products (AGEs) affect the follicular microenvironment. The close relationship between AGEs, proinflammatory cytokine production and activation of the unfolded protein response (UPR), which involves activating transcription factor 4 (ATF4), is crucial for regulation of various cellular functions...

journal_title：Molecular human reproduction

authors： Takahashi N,Harada M,Azhary JMK,Kunitomi C,Nose E,Terao H,Koike H,Wada-Hiraike O,Hirata T,Hirota Y,Koga K,Fujii T,Osuga Y

更新日期：2019-11-30 00:00:00

abstract：:The aim of the present study was to investigate the potential role of Toll-like receptor 4 (TLR4) in lipopolysaccharide (LPS)-induced preterm delivery. Intraperitoneal injection of LPS in the presence or absence of previous TLR4 blockade was performed to establish a murine model of preterm delivery. The incidences of ...

journal_title：Molecular human reproduction

authors： Li L,Kang J,Lei W

更新日期：2010-04-01 00:00:00

abstract：:Testis expressed gene 18 (Tex18) is a small gene with one exon of 240 bp, which is specifically expressed in male germ cells. The gene encodes for a protein of 80 amino acids with unknown domain. To investigate the function of (Tex18) gene, we generated mice with targeted disruption of the (Tex18) gene by homologous r...

journal_title：Molecular human reproduction

authors： Jaroszynski L,Dev A,Li M,Meinhardt A,de Rooij DG,Mueller C,Böhm D,Wolf S,Adham IM,Wulf G,Engel W,Nayernia K

更新日期：2007-03-01 00:00:00

abstract：:The preimplantation period of life in mammals encompasses a tremendous amount of restructuring and remodeling of the embryonic genome and reprogramming of gene expression. These vast changes support metabolic activation and cellular processes that drive early cleavage divisions and enable the creation of the earliest ...

journal_title：Molecular human reproduction

authors： Schall PZ,Ruebel ML,Midic U,VandeVoort CA,Latham KE

更新日期：2019-03-01 00:00:00

abstract：:The expression of insulin-like growth factor-I (IGF-I) was measured at the mRNA and protein level in myometrium and fibroids from women with and without preoperative treatment with a gonadotrophin-releasing hormone (GnRH) agonist for 3 months, from post-menopausal women, from pregnant women and in myometrium from wome...

journal_title：Molecular human reproduction

authors： Englund K,Lindblom B,Carlström K,Gustavsson I,Sjöblom P,Blanck A

更新日期：2000-10-01 00:00:00

abstract：:Nuclear oligomerization domains (NODs) are cytosolic pattern recognition receptors (PRRs), present in epithelial cells, monocytes and dendritic cells. This study details their expression, regulation and role in human endometrium. Real-time PCR showed that NOD1 mRNA is constitutively expressed in endometrium. NOD2 is u...

journal_title：Molecular human reproduction

authors： King AE,Horne AW,Hombach-Klonisch S,Mason JI,Critchley HO

更新日期：2009-05-01 00:00:00

abstract：:To mimic post-ovulatory ageing, we have extended the in vitro maturation (IVM) phase to 48 h and examined effects on (i) developmental potential, (ii) expression of a panel of developmentally important genes and (iii) gene-specific epigenetic marks. Results were compared with the 24 h IVM protocol (control) usually em...

journal_title：Molecular human reproduction

authors： Heinzmann J,Mattern F,Aldag P,Bernal-Ulloa SM,Schneider T,Haaf T,Niemann H

更新日期：2015-10-01 00:00:00

abstract：:Fluorescent in situ hybridization (FISH) studies of human preimplantation embryos have demonstrated a high proportion of chromosomal mosaicism. To investigate the different timings and nature of chromosomal mosaicism, we developed single cell multiplex fluorescent (FL)-PCR to distinguish meiotic and mitotic cell divis...

journal_title：Molecular human reproduction

authors： Katz-Jaffe MG,Trounson AO,Cram DS

更新日期：2004-02-01 00:00:00